CN103041381A - Recombinant inhibin vaccine and preparation method and application of recombinant inhibin vaccine - Google Patents
Recombinant inhibin vaccine and preparation method and application of recombinant inhibin vaccine Download PDFInfo
- Publication number
- CN103041381A CN103041381A CN2013100072630A CN201310007263A CN103041381A CN 103041381 A CN103041381 A CN 103041381A CN 2013100072630 A CN2013100072630 A CN 2013100072630A CN 201310007263 A CN201310007263 A CN 201310007263A CN 103041381 A CN103041381 A CN 103041381A
- Authority
- CN
- China
- Prior art keywords
- inhibin
- restructuring
- vaccine
- preparation
- protein subunit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention relates to a recombinant inhibin vaccine which is characterized in that the recombinant inhibin vaccine is oil emulsion prepared by mixing an oleic preparation and an aqueous preparation at a volume ratio of 2:1, wherein the oleic preparation comprises 4% of span 80, 2% of span 85 and No. 10 mineral oil; the aqueous preparation comprises sterilization tween 80, recombinant inhibin alpha subunit protein and sterile distilled water; the recombinant inhibin alpha subunit protein is obtained after an expression vector pREST-INH (Probable Recombinant Protein Epitope Signature Tag-Iso Nicotinyl Hydrazide) is expressed by an escherichia coli expressed strain BL21 (DE3) and purified; the content of the recombinant inhibin alpha subunit protein in the aqueous preparation is 0.5-10mg/mL; and the content of the sterilization tween 80 in the aqueous preparation is 4%. The recombinant inhibin vaccine is obtained by mixing the oleic preparation and the standby aqueous preparation at the volume ratio of 2:1. Female livestock undergoes intramuscular injection during application, and the reproductive capacity of the livestock is improved by the immunity of the recombinant inhibin alpha subunit protein.
Description
Technical field
The present invention relates to a kind of inhibin vaccine and its preparation method and application, relate to a kind of promotion domestic animal and oestrus and improve livestock reproduction power vaccine and its preparation method and application, belong to the animal thremmatology field.
Background technology
The height of livestock reproduction power directly has influence on the economic benefit of cultivation and animal husbandry.The part domestic animal be subject to summer high temperature stress, the impact of the factor such as lactogenic, drylot feeding environment, season and management, cause the regulative mechanism of hypothalamic-pituitary-gonadal regulating shaft disorderly, make hypophysis can not secrete enough promoting sexual gland hormone to promote the ripe and ovulation of follicular development, cause ovary to be in relative static conditions, and cause the decline of reproductive performance so that it shows as weary feelings.At present, in actual production, adopt exogenous hormone (such as promoting sexual gland hormone, estrogen and prostaglandin etc.) to process more, oestrus to promote female animal follicular development and performance, then breeding makes it to finish breeding activity.Yet above three kinds of application are hormone more widely, its mechanism of action is simple or the effect link is less, as short gonad parahormone can only affect follicular development and because the half-life is short the persistent period short and to the raising of animal conception rate or keep less, estrogen and prostaglandins be several functions without promoting breeding activities then.Therefore, the animal reproduction research worker is constantly studying to develop novel genital regulating new technique and new product, hope with comparatively simple program promote that well gonad is movable, oestrus, breeding, fetal development, reproductive tract grow embryo nidation be impregnated, thereby improve the reproductive performance of animal.
Studies show that in a large number the follicular development of female animal is promoted by promoting sexual gland hormone, also is subject to but the impact of some local modulation factors simultaneously.For example, inhibin (INH)-activin (ACT)-follistatin (FS) system participates in the interior local regulatory factor of important ovary that follicular development is regulated.The preovulatory phase dominant follicle secretion a large amount of inhibin (INH) suppress the secretion of hypophysis FSH by negative feedback mechanism, inhibin and follistatin also have the growth of the little follicle of direct inhibition or laggard follicle simultaneously.By initiatively or in the passive immunity inhibin and the biological activity of endogenous inhibin, then can promote on the one hand the secretion of FSH, remove again on the other hand inhibin to inhibition or the infringement of follicle in growing.
Therefore, before people's research is found, behind the active immunity inhibin, by promoting follicular development to promote follicle secretion more estradiol (E2), thereby make female animal oestrus the performance more vigorous, well-developed follicle is converted into the progesterone (P4) of high-quality corpus luteum and secreting high levels after ovulation.The E2 of master's level affects environment in the female reproductive tract when oestrusing, and helps the operation of sperm and the combination of smart ovum.Corpus luteum is grown the progesterone of secretion after the ovulation, then further by the impact on intrauterine secretion environment, promotes embryo's growth.And our research finds that also it is the higher embryo of quality that anti-inhibin antibody can promote the ovum of maturation in vitro to make it to grow after fertilization.And above each side event all helps to improve the conception rate of animal breeding, also namely improves the reproductive performance of animal.
Summary of the invention
The object of the invention is to, provide a kind of and can promote female domestic animal to oestrus and improve restructuring inhibin vaccine of livestock reproduction power and its preparation method and application.
The object of the present invention is achieved like this: a kind of restructuring inhibin vaccine, it is characterized in that: it is the oil emulsion that is mixed according to the volume ratio of 2:1 by oil phase formulation and water preparation, oil phase formulation by department this 80, this 85 and No. 10 mineral oil of department form, wherein contain this department of 80,2% of department of 4% this 85; The water preparation is comprised of sterilization Tween 80, restructuring inhibin α protein subunit and aquesterilisa, described restructuring inhibin α protein subunit is by expression vector pREST-INH, behind escherichia coli expression bacterial strain BL21 (DE3) expression and purification, obtain, contain 4% sterilization Tween 80 in the water preparation.
In the present invention: the content of restructuring inhibin α protein subunit is 2mg/mL or 1mg/mL or 0.5mg/mL or 0.25mg/mL in the vaccine.
A kind of preparation method of above-mentioned restructuring inhibin vaccine is characterized in that:
A) this department of 80,2% of 4% department this 85 with No. 10 mineral oil refiners on 3000rpm mixed 20 minutes, autoclaving under 121 ℃ of conditions, the acquisition oil phase formulation, for subsequent use;
B) by expression vector pREST-INH, express and purification acquisition restructuring inhibin α protein subunit through escherichia coli expression bacterial strain BL21 (DE3), Tween 80 was sterilized 15 minutes under 121 ℃ of conditions, get Tween 80 and restructuring inhibin α protein subunit 3000rpm on refiner after sterile distilled water, the sterilization, form the water preparation behind 20 minutes mixings, for subsequent use; The content of restructuring inhibin α protein subunit is 0.5 ~ 10mg/mL in the water preparation, and the content of sterilization Tween 80 is 4%;
C) oil phase formulation for subsequent use and water preparation for subsequent use are mixed according to the volume ratio of 2:1, concrete steps are: first oil phase formulation is placed refiner 3000rpm mixing, progressively add water preparation for subsequent use in the blending process, after whole water preparations add, continued mixing 20 minutes; Obtain restructuring inhibin vaccine, the content of restructuring inhibin α protein subunit is 2mg/mL or 1mg/mL or 0.5mg/mL or 0.25mg/mL in this vaccine.
A kind of application of above-mentioned restructuring inhibin vaccine is characterized in that: after female domestic animal intramuscular injection, by the immunity raising livestock reproduction power of restructuring inhibin α protein subunit.
In the application of described restructuring inhibin vaccine: described female domestic animal is sow or ewe or water cow.
The invention has the advantages that: adopt method provided by the present invention, after utilizing the restructuring inhibitant immunization, can effectively treat the kind female animal of obstinate non-estrus, can impel oestrusing of female animal, improve significantly female animal oestrus rate and the performance of oestrusing, be used for solving that oestrusing of female animal postponed or the problem of non-estrus, utilize the technology of the present invention can significantly reduce drain on manpower and material resources, reduce production costs, and produced great economic benefit and social effect.
Description of drawings
Fig. 1 is the restructuring inhibin vaccine-induced Anestrus sows sketch map of oestrusing.
Among the figure, solid line is immune group, and dotted line is matched group; The arrow of top represents the time of intramuscular injection, and expression experiment beginning is the 1st day, the 21st day and the 42nd day respectively.
The specific embodiment
Embodiment 1
The preparation of restructuring inhibin vaccine
Material:
Department's basis 80, these 85, No. 10 mineral oil of department, Tween 80 (the equal outsourcing of above material), sterile distilled water.
Restructuring inhibin α protein subunit: by expression vector pREST-INH, express and purification through escherichia coli expression bacterial strain BL21 (DE3).
Preparation process:
This department of 80,2% of 4% department this 85 with No. 10 mineral oil refiners on 3000rpm mixed 20 minutes, autoclaving is 15 minutes under 121 ℃ of conditions, the acquisition oil phase formulation is for subsequent use; By expression vector pREST-INH, express and purification acquisition restructuring inhibin α protein subunit through escherichia coli expression bacterial strain BL21 (DE3), Tween 80 was sterilized 15 minutes under 121 ℃ of conditions, get Tween 80 and restructuring inhibin α protein subunit 3000rpm on refiner after sterile distilled water, the sterilization, mixing formed the water preparation in 20 minutes, restructuring inhibin α protein subunit content in the water preparation is 0.5 ~ 10mg/mL, and the content of sterilization Tween 80 is 4%, and is for subsequent use.
Oil phase formulation for subsequent use and water preparation for subsequent use are mixed according to the volume ratio of 2:1, concrete steps are: first oil phase formulation is placed refiner 3000rpm mixing, progressively add water preparation for subsequent use in the blending process, after whole waters add, continued mixing 20 minutes.Obtain restructuring inhibin vaccine.In the restructuring inhibin vaccine content of restructuring inhibin α protein subunit to be 2mg/mL or 1mg/mL or 0.5mg/mL or 0.25mg/mL(change content in the restructuring inhibin vaccine by the content that changes the restructuring inhibin α protein subunit in the water preparation).
Embodiment 2
The restructuring inhibin induces Anestrus sows to oestrus
Immune group
Reagent chemicals: restructuring inhibin vaccine (content of restructuring inhibin α protein subunit is 0.5mg/mL and 0.25mg/mL in the restructuring inhibin vaccine, is provided by embodiment 1)
Matched group
Reagent chemicals: injection normal saline (outsourcing)
Experimentation
Select the body condition developmental state similar because summer high temperature weather causes 18 of the adult Anestrus sows of long-time obstinate non-estrus, be divided at random two groups, 9 every group.Design every three all intramuscular injection inhibin vaccine of once recombinating, is used the vaccine 1mL of 0.5mg/mL to the sow of immune group for the first time, for the second time and use for the third time the vaccine 1mL of 0.25mg/mL to measure identical.Matched group is every three all intramuscular injection shot normal saline, each 1mL.After the injection, the individuality of oestrusing appears in immune group and matched group, namely breeds, and residue non-estrus colony proceeds vaccine injection.Induce the Anestrus sows process of oestrusing to see Fig. 1, the arrow of top represents the time of intramuscular injection among the figure, is respectively experiment beginning the 1st day, the 21st day and the 42nd day.As seen from Figure 1,1 ~ 2 restructuring of 9 sow intramuscular injection of immune group inhibin vaccine, the rate of oestrusing just reaches 100%.After finishing three intramuscular injection in 9 pigs of matched group, only have 3 hair feelings, the rate of oestrusing only is 33.3%.The number born of sow of oestrusing is carried out tracing record and statistics, and the immune group average litter size is 12.25 ± 1.03, and the matched group average litter size is 9.3 ± 0.67, and both compare, and significant difference (P＜0.05) is such as table 1.
Table 1: immune IHN oestruses on Anestrus sows and the impact of the litter size of breeding
| Group | Quantity n | The sow number of oestrusing | The rate of oestrusing (%) | The average subnumber that produces |
| Immune group | 9 | 9 | 100 | 12.25±1.03 a |
| Matched group | 9 | 3 | 33.3 | 9.30±0.67 b |
Embodiment 3
The restructuring inhibin induces weary feelings ewe to oestrus
Immune group
Reagent chemicals: restructuring inhibin vaccine (content of restructuring inhibin α protein subunit is 1mg/mL in the restructuring inhibin vaccine, is provided by embodiment 1); Progesterone slow release vaginal suppository (outsourcing).
Matched group
Reagent chemicals: progesterone slow release vaginal suppository (outsourcing).
Experimentation
Select 30 ewes of the same terms to be divided at random two groups, one group is immune group, and another group is matched group.
Immune group was on the same day of vagina heeling-in progesterone vaginal suppository, and 1 milliliter of intramuscular injection restructuring inhibin vaccine removes vagina and fastens when the 12nd day of heeling-in progesterone vaginal suppository, then observed ewe oestrus performance and conception rate.
Matched group removes vagina and fastens only according to traditional approach heeling-in progesterone vaginal suppository when the 12nd day of heeling-in progesterone vaginal suppository, then observe ewe oestrus the performance and conception rate.
The result shows that what performance was oestrused in 15 ewes of immune group is 12, and the estrus synchronization rate reaches 80%; And in 15 ewes of matched group the performance oestrus only have 5, the estrus synchronization rate is 33%.In addition, the performance of oestrusing of immune group ewe is all vigorous especially and obvious, significantly is better than the oestrus performance of ewe of matched group.The result shows that immunosuppressant can significantly improve the estrus synchronization rate of ewe.
Embodiment 4
The restructuring inhibin induces weary feelings water cow to oestrus
Immune group
Reagent chemicals: restructuring inhibin vaccine (content of restructuring inhibin α protein subunit is 2mg/mL in the restructuring inhibin vaccine, is provided by embodiment 1); Cloprostenol PGF2 α (outsourcing); Gonadotropin releasing hormone GnRH(or No. 3 LRH-A3 of induced ovulation element) (outsourcing).
Matched group
Reagent chemicals: Cloprostenol PGF2 α (outsourcing); Gonadotropin releasing hormone GnRH(or No. 3 LRH-A3 of induced ovulation element) (outsourcing).
Experimentation
Select 24 water cows of the same terms to be divided at random two groups, wherein immune group is 11,13 of matched groups.
Immune group was on same day that Estrus synchronization begins, intragluteal injection content is the restructuring inhibin vaccine of 2mg/mL, and dosage is 2mg/ml/head, simultaneously intramuscular injection gonadotropin releasing hormone GnRH, dosage is 2ml/ head (or No. 3 LRH-A3 of induced ovulation element, dosage is 100 μ g/ heads); Inject Cloprostenol PGF2 α after 7 days, dosage is the 2ml/ head; After 2 days, intramuscular injection gonadotropin releasing hormone GnRH again, dosage are 2ml/ head (or No. 3 LRH-A3 of induced ovulation element, dosage is 100 μ g/ heads); Observe the situation of the oestrusing pedestrian worker's fertilization of going forward side by side after 15-22 hour.
Matched group was on the same day that Estrus synchronization begins, and intragluteal injection gonadotropin releasing hormone GnRH, dosage are 2ml/ head (or No. 3 LRH-A3 of induced ovulation element, dosage is 100 μ g/head); Inject Cloprostenol PGF2 α after 7 days, dosage is the 2ml/ head; After 2 days, intramuscular injection gonadotropin releasing hormone GnRH again, dosage are 2ml/ head (or No. 3 LRH-A3 of induced ovulation element, dosage is 100 μ g/head); Observe the situation of the oestrusing pedestrian worker's fertilization of going forward side by side during 15-22 hour.
Oestrus and identify and the artificial fertilization is opportunity, find cow vaginal orifice congested swelling, vaginal mucosa flushing, have mucus to flow out and mutually during mounting, the artificial fertilization carried out twice at 72 hours and 96 hours respectively.Motility rate is more than 30% after the used thawing of semen, and semen dosage is 1 (0.25ml).The semen deposition mode is that rectum is the semen deposition of hold deep.Nonoperative method rushes embryo after 7 days, the record number of eggs ovulated.Breed and carried out conception rate with B ultrasonic in rear 90 days and detect.Experimental result is recorded in the table 2, can obviously be found out by table 2, and restructuring inhibin vaccine can significantly improve ovulation rate and the conception rate behind the water cow estrus synchronization.
Table 2: immunosuppressant significantly improves ovulation rate and the conception rate behind the water cow estrus synchronization
| Group | Quantity | Number of eggs ovulated (%) | Breeding was impregnated in rear 90 days counts (%) |
| Matched group | 13 | 7（53%） | 2（15.4%） |
| Immune group | 11 | 10（90.9%） | 5(45.5%) |
More than each embodiment be not to concrete restriction of the present invention, as long as the disclosed content in the description according to the present invention, in conjunction with basic general knowledge or the customary means of this area, the extension of the unsubstantiality that the present invention is made does not all exceed the category that claim of the present invention limits.
Claims (6)
1. restructuring inhibin vaccine, it is characterized in that: it is the oil emulsion that is mixed according to the volume ratio of 2:1 by oil phase formulation and water preparation, oil phase formulation by department this 80, this 85 and No. 10 mineral oil of department form, wherein contain this department of 80,2% of department of 4% this 85; The water preparation is comprised of sterilization Tween 80 and restructuring inhibin α protein subunit and sterile distilled water, described restructuring inhibin α protein subunit is by expression vector pREST-INH, behind escherichia coli expression bacterial strain BL21 (DE3) expression and purification, obtain, the content of restructuring inhibin α protein subunit is 0.5 ~ 10mg/mL in the water preparation, and the content of sterilization Tween 80 is 4%.
2. restructuring inhibin vaccine according to claim 1 is characterized in that: the content of restructuring inhibin α protein subunit is 2mg/mL or 1mg/mL or 0.5mg/mL or 0.25mg/mL in the restructuring inhibin vaccine.
3. the preparation method of the described restructuring inhibin of claim 1 vaccine is characterized in that:
A) this department of 80,2% of 4% department this 85 with No. 10 mineral oil refiners on 3000rpm mixed 20 minutes, autoclaving is 15 minutes under 121 ℃ of conditions, the acquisition oil phase formulation is for subsequent use;
B) by expression vector pREST-INH, express and purification acquisition restructuring inhibin α protein subunit through escherichia coli expression bacterial strain BL21 (DE3), Tween 80 was sterilized 15 minutes under 121 ℃ of conditions, get Tween 80 and restructuring inhibin α protein subunit 3000rpm on refiner after sterile distilled water, the sterilization, form the water preparation behind 20 minutes mixings, for subsequent use; Restructuring inhibin α protein subunit content in the water preparation is 0.5 ~ 10mg/mL, and the content of sterilization Tween 80 is 4%;
C) oil phase formulation for subsequent use and water preparation for subsequent use are mixed according to the volume ratio of 2:1, concrete steps are: first oil phase formulation is placed refiner 3000rpm mixing, progressively add water preparation for subsequent use in the blending process, after whole water preparations add, continued mixing 20 minutes; Obtain restructuring inhibin vaccine.
4. the preparation method of described restructuring inhibin vaccine according to claim 3, it is characterized in that: the content that obtains restructuring inhibin α protein subunit in the restructuring inhibin vaccine is 2mg/mL or 1mg/mL or 0.5mg/mL or 0.25mg/mL.
5. the application of a claim 1 or 2 described restructuring inhibin vaccines is characterized in that: after female domestic animal intramuscular injection, the immunity by restructuring inhibin α protein subunit improves livestock reproduction power.
6. the according to claim 5 application of described restructuring inhibin vaccine is characterized in that: described female domestic animal is sow or ewe or water cow.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100072630A CN103041381A (en) | 2013-01-09 | 2013-01-09 | Recombinant inhibin vaccine and preparation method and application of recombinant inhibin vaccine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100072630A CN103041381A (en) | 2013-01-09 | 2013-01-09 | Recombinant inhibin vaccine and preparation method and application of recombinant inhibin vaccine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103041381A true CN103041381A (en) | 2013-04-17 |
Family
ID=48054390
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100072630A Pending CN103041381A (en) | 2013-01-09 | 2013-01-09 | Recombinant inhibin vaccine and preparation method and application of recombinant inhibin vaccine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103041381A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111134084A (en) * | 2020-01-06 | 2020-05-12 | 浙江省农业科学院 | Method for optimizing timing insemination effect of replacement gilts |
| CN114948325A (en) * | 2021-12-31 | 2022-08-30 | 江苏省农业科学院 | Follicle development treatment method for improving cow mating conception rate |
| CN116375844A (en) * | 2023-02-22 | 2023-07-04 | 四川农业大学 | Inhibin antigen peptide, vaccine and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000025831A1 (en) * | 1998-11-03 | 2000-05-11 | Federman & Sons (Holdings) Ltd. | A new composition and method for increasing production yield of agriculturally useful species |
| CN1569230A (en) * | 2003-11-05 | 2005-01-26 | 华南农业大学 | Preparation and application of a gene engineering vaccine of muscle growth retarder |
| CN101423833A (en) * | 2008-10-21 | 2009-05-06 | 华南农业大学 | Method for preparing inhibin genetic engineering regulation antigen |
| CN102166348A (en) * | 2010-12-31 | 2011-08-31 | 华南农业大学 | Application of inhibin recombinant fusion protein to preparing medicines for promoting oestrus and hybridization of sows |
-
2013
- 2013-01-09 CN CN2013100072630A patent/CN103041381A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000025831A1 (en) * | 1998-11-03 | 2000-05-11 | Federman & Sons (Holdings) Ltd. | A new composition and method for increasing production yield of agriculturally useful species |
| CN1569230A (en) * | 2003-11-05 | 2005-01-26 | 华南农业大学 | Preparation and application of a gene engineering vaccine of muscle growth retarder |
| CN101423833A (en) * | 2008-10-21 | 2009-05-06 | 华南农业大学 | Method for preparing inhibin genetic engineering regulation antigen |
| CN102166348A (en) * | 2010-12-31 | 2011-08-31 | 华南农业大学 | Application of inhibin recombinant fusion protein to preparing medicines for promoting oestrus and hybridization of sows |
Non-Patent Citations (1)
| Title |
|---|
| 王世凯: "广西巴马小型猪Myostatin和Leptin基因的克隆 、表达及动物免疫实验", 《广西大学硕士学位论文》, 10 December 2008 (2008-12-10) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111134084A (en) * | 2020-01-06 | 2020-05-12 | 浙江省农业科学院 | Method for optimizing timing insemination effect of replacement gilts |
| CN114948325A (en) * | 2021-12-31 | 2022-08-30 | 江苏省农业科学院 | Follicle development treatment method for improving cow mating conception rate |
| CN116375844A (en) * | 2023-02-22 | 2023-07-04 | 四川农业大学 | Inhibin antigen peptide, vaccine and preparation method thereof |
| CN116375844B (en) * | 2023-02-22 | 2024-03-29 | 四川农业大学 | Inhibin antigen peptide, vaccine and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| de Oliveira Marques et al. | Influence of category-heifers, primiparous and multiparous lactating cows-in a large-scale resynchronization fixed-time artificial insemination program | |
| CN104012466B (en) | Promote that ox oestruses and improves the method for ox conception rate | |
| Abdalla et al. | Effect of progestagen, PGF2α, PMSG and GnRH on estrus synchronization and some reproductive and productive traits in Barki ewes | |
| Dogan et al. | Estrous synchronization during the natural breeding season in Anatolian black does | |
| Ryan et al. | Pregnancy rates in dairy cows following the administration of a GnRH analogue at the time of artificial insemination or at mid-cycle post insemination | |
| CN103041381A (en) | Recombinant inhibin vaccine and preparation method and application of recombinant inhibin vaccine | |
| Vikrama et al. | Use of assisted reproductive technologies for livestock development | |
| Zhao et al. | Efficiency of methods applied for goat estrous synchronization in subtropical monsoonal climate zone of Southwest China | |
| CN105248368B (en) | A method of promoting the double calves of beef cattle production | |
| Gümen et al. | Effects of GnRH, PGF2α and oxytocin treatments on conception rate at the time of artificial insemination in lactating dairy cows. | |
| Pangestuningrum et al. | Etawa goat estrus quality with estrus synchronization | |
| Malik et al. | Effects of timed artificial insemination following estrus synchronization in postpartum beef cattle | |
| Hussein et al. | Effect of different doses of FSH on superovulation, production and quality of embryo in North Omani Cattle breed | |
| Omontese | Estrus synchronization and artificial insemination in goats | |
| Nayak et al. | Induction of oestrus in true anestrus buffaloes using Crestar implant alone and in combination with PMSG | |
| Ngula et al. | A novel semen supplement (SuinFort) improves sow fertility after artificial insemination | |
| Berean et al. | Comparative data about estrus induction and pregnancy rate on Lacaune ewes in non-breeding season after melatonin implants and intravaginal progestagen | |
| Dhaka et al. | Efficacy of modified co-synch plus protocol with or without progesterone device for estrus induction and conception rate in Murrah buffaloes under field conditions during summer season | |
| Găvan et al. | Age of first calving and subsequent fertility and survival in Holstein Friesian cattle | |
| Stoycheva et al. | Induction of synchronic oestrus, impregnancy and fertility of female lambs at 18 months of age and female lambs at 7-8 months of age, after treatment with PMSG | |
| Savani et al. | Estrus Induction and fertility response following ovsynch alone and ovsynch plus prid protocol in postpartum anestrus Surti buffaloes | |
| Turk et al. | Effects of semen sexing kits (HeiferplusTM and BullplusTM) supplemented to frozen-thawed bull semen on pregnancy rates, foetal sex ratios and selected reproductive parameters in cows. | |
| Bhattacharyya et al. | Treatment of delayed ovulation in dairy cattle | |
| Peltoniemi et al. | Feeding affects reproductive performance and reproductive endocrinology in the gilt and sow | |
| Karikari et al. | Reproductive response of West African dwarf does to prostaglandin administration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130417 |