CN103033482A - Full-automatic determining instrument of red blood cell osmotic fragility - Google Patents
Full-automatic determining instrument of red blood cell osmotic fragility Download PDFInfo
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- CN103033482A CN103033482A CN2012105649993A CN201210564999A CN103033482A CN 103033482 A CN103033482 A CN 103033482A CN 2012105649993 A CN2012105649993 A CN 2012105649993A CN 201210564999 A CN201210564999 A CN 201210564999A CN 103033482 A CN103033482 A CN 103033482A
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a full-automatic determining instrument of red blood cell osmotic fragility. The full-automatic determining instrument is based on the principle of light-proof turbidimetry and consists of a sample absorbing device, an automatic electromechanical device, a light detector and a circuit system, wherein the sample absorbing device is used for absorbing a blood sample through the instrument; the automatic electromechanical device is used for accomplishing various control actions in the automatic measurement of the instrument; the light detector is used for radiating reaction liquid to generate a permeable light signal; the circuit system is used for controlling various stepper motors, electromagnetic valves and peristaltic pumps, converting the light signal into an electric signal, amplifying the electric signal and converting the electric signal into a digital signal so as to carry out data processing analysis, data display and communication between the instrument and a PC (Personal Computer). The full-automatic determining instrument is convenient to operate, rapid in measuring speed, high in precision and good in repeatability, and the information of the red blood cell osmotic fragility of the blood sample can be obtained through full-automatic detection.
Description
Technical field
The present invention relates to a kind of full-automatic determining instrument of osmotic fragility, this instrument can be used for the examination of the small cell anemias such as clinical thalassemia.
Background technology
Human erythrocyte accounts for 40% of whole blood cumulative volume, mainly is comprised of 64% moisture and 32% haemoglobin.Red blood cell is in hypotonic salt solusion, and when water permeated its inside and reaches to a certain degree, red blood cell expanded and breaks, and haemoglobin overflows and namely is called hypo-osmoticity haemolysis in the cell.Erythrocyte membrane is called osmotic fragility to the resistibility of hypotonic salt solusion haemocylolysis.Haemoglobin generates and reduces in thalassemia, the Patients with iron deficiency anemia red blood cell, cell volume is less, osmotic fragility calibration ordinary person obviously reduces, so osmotic fragility is usually used in the small cell anemias such as examination thalassemia, hypoferric anemia clinically.
At present the osmotic fragility assay method is mainly contained two kinds:
(1) erythrocyte osmotic fragility test: this method is to measure the conventional method of osmotic fragility, its principle be red blood cell in hypotonic salt solusion, moisture enters in the cell by cell membrane, makes it swelling fracture and haemolysis.This test is to measure red blood cell to the resistibility of the hypotonic salt solusion of variable concentrations, and red blood cell is relevant with the ratio of its surface area and volume to the resistibility of hypotonic salt solusion, and ratio is larger, and then resistibility is larger, and fragility is lower; Otherwise then resistibility is less, and fragility is higher.The method of judged result is during mensuration: leave standstill 2h in room temperature after, upper solution begins to occur transparent redness and the pipe end person that has the red blood cell is beginning haemolysis pipe; Solution is transparent redness, and the pipe end is the complete hemolysis pipe without the red blood cell person fully.
The quantitative examination test of (2) one pipes: this method is the determination method that improves on the basis of conventional erythrocyte osmotic fragility test, is the topmost detection method of present sanitation system.Its principle is that red blood cell is in a certain specific hypotonic salt solusion, under the uniform temperature condition, after the certain hour effect, measure the percent (being hemolysis rate) that the concentration of haemoglobin in solution that produces behind the red blood cell generation haemolysis accounts for full haemolysis hemoglobin concentration, reflect osmotic fragility.The hemolysis rate that mensuration draws is higher, and erythrocyte number reduces more, shows that osmotic fragility is higher, otherwise lower.The method of judged result was when this method was measured: use spectrophotometer and carry out colorimetric analysis, and with haemoglobin maximum absorption wavelength 530nm, the absorbance of assaying reaction liquid, the size of this absorbance is directly proportional with the concentration of haemoglobin in the reactant liquor.
Above-mentioned two kinds of methods all need manual operations, and complex operation is consuming time, is not suitable for clinical mass detection.Conventional method needs the naked eyes judged result, affected by artificial subjective factor, causes result's poor accuracy; The quantitative examination test of one pipe is owing to other composition that exists simultaneously in the reactant liquor in undissolved red blood cell and free hemoglobin and the blood, could carry out accurately colorimetric estimation to the result after must be centrifugal, so can't realize course of reaction is done dynamically to measure and full-automatic the analysis.Therefore, have not yet to see the instrument of automatic assay osmotic fragility.
Summary of the invention
The object of the invention is to invent that a kind of measuring speed is fast, precision is high, the full-automatic osmotic fragility determining instrument of good reproducibility.For achieving the above object, the present invention adopts light tight kinetic turbidimetric assay to measure: with the hypotonic salt solusion mixing of blood sample and specific osmotic pressure, with lyse red blood cell not as the main particle that consists of this mixed liquor turbidity, select a kind of not lyse red blood cell that it is had higher absorbance, from the solubility coloring matters such as free hemoglobin of red blood cell stripping to its specific wavelength that weak absorbance is arranged as measuring wavelength, the variation of transmittance before and after the free haemoglobin stripping calculates the relative hemolysis rate value that can judge osmotic fragility in the assaying reaction liquid.Just be based on above-mentioned principle, the present invention just is achieved full-automatic the detection.
The present invention includes a kind of full-automatic determining instrument of osmotic fragility, it is characterized in that: comprise a base, the base front end is furnished with two rightabout heparin tube conveyer mechanisms for delivery of support of blood collecting pipe, its middle being provided with is inhaled the sample position; Base postmedian both sides are provided with for delivery of the cuvette conveyer mechanism of multi-joint cuvette and useless cuvette and collect the position, the centre is the gear train bearing support, the gear train bearing support is provided with initial bit, sample absorbing device is installed on the described gear train bearing support, described sample absorbing device comprises a suction tube and is used for the suction sample support piece of its outer wall washing, the plane motion of form before and after this sample absorbing device can be done up and down by the control of stepper motor; The place of pressing close to inhale the sample position under the gear train bearing support is provided for shining the photodetector that reactant liquor produces the permeability light signal.
The Main Function of gear train bearing support is to carry sample absorbing device to move.Suction tube is preferably steel, and for ease of the puncture blood collecting pipe, its tip designs is circle inclined-plane piercing needle; For reducing to greatest extent the possibility of the sticking blood of outside surface, its outside surface is covered with the teflon anti-stick coating.Inhale sample and support the block-shaped rectangular parallelepiped that is preferably, the center has the vertical passageway, the internal diameter of described vertical passageway is slightly larger than the suction tube external diameter, the side, position of its top about 20% has interconnection, described interconnection links to each other with solenoid valve and peristaltic pump by pipeline, both can play the vertical effect of suction tube that keeps, and can introduce the outside surface that pure water cleans suction tube by the interconnection on it again.Suction tube passes inhales the vertical passageway that sample is supported piece, and the upper end links to each other with solenoid valve and peristaltic pump by rubber tubing, can realize inhaling the effect of cleaning in sample and the pipe.
Photodetector comprises for generation of the light source of light signal and the receiver of receiving optical signals, both relative spacings are slightly larger than the position of a cuvette external diameter, the pushing block of cuvette conveyer mechanism is advanced into multi-joint cuvette between described light source and the described receiver first during use, light source starts after the reaction beginning, and received device was collected after light signal passed cuvette light face.According to architectural feature and the experiment screening of human erythrocyte, the wavelength of light signal can be chosen to be 840～1200nm.
The heparin tube number that the ware number of multi-joint cuvette and support of blood collecting pipe arrange is corresponding, and the equal built-in an amount of NaCl-PBS damping fluid of each ware of multi-joint cuvette.
The present invention also comprises: be used for various stepper motors, sample presentation belt and the sample presentation pushing block of transmission cuvette, test tube rack and sample absorbing device, be used for the various solenoid valves of pipeline-cleaning, air blowing, the peristaltic pump of be used for suction, blowing, taking a sample.For guaranteeing test result reliability, corresponding outer cover also can be installed with the protection instrument.
The present invention also has a perfect control, data processing and analysis, demonstration, communication circuitry, be used for finishing the various stepper motors of control, solenoid valve, peristaltic pump, convert light signal to electric signal, amplify, be converted to again digital signal, carry out communicating by letter between Data Management Analysis, data demonstration, instrument and PC or the miscellaneous equipment.
Determination step of the present invention is as follows: the heparin tube conveyer mechanism is transported to suction sample position by the support of blood collecting pipe that pushing block will carry heparin tube, the cuvette conveyer mechanism is transported to the photodetector position with cuvette simultaneously, suction tube moves to inhaling the sample position from initial bit, from moving to again first ware top of multi-joint cuvette after first heparin tube is inhaled sample, quantitative blood sample is injected cuvette, and air blowing mixing (hybrid mode is not limited to blow); The liquid turbidity that photodetector begins to detect in the cuvette changes, and microprocessor calculates the relative hemolysis rate in the setting-up time, and meanwhile suction tube returns initial bit and cleans; After detect finishing, each moves the position of a unit to pushing block with multi-joint cuvette and support of blood collecting pipe, begins next test; After whole row's heparin tube and the test of multi-joint cuvette were finished, the heparin tube conveyer mechanism was taken support of blood collecting pipe output away, and pushing block is pushed into useless cuvette with multi-joint cuvette and collects the position.
The present invention has following beneficial effect: adopt the turbidity that not molten red blood cell produces in the light tight kinetic turbidimetric assay detection reaction liquid, need not centrifugally operated, therefore can do dynamically to measure to whole course of reaction, and can obtain more information about osmotic fragility by the dynamic response tracing analysis, thereby whole mensuration flow process can realize full-automation, detection speed is fast, degree of accuracy is high, good reproducibility, shows in real time to detect data; Required blood sample amount is few, can reduce blood sampling volume; Use the photoelectric detective circuit testing result, need not naked eyes and judge, avoid to greatest extent artificial subjective factor impact.
Description of drawings
Fig. 1 is the one-piece construction synoptic diagram of instrument of the present invention.
Fig. 2 is the diagrammatic cross-section of photodetector of the present invention.
Fig. 3 is that the present invention inhales the diagrammatic cross-section that sample is supported piece.
Embodiment
The invention will be further described below in conjunction with drawings and Examples.
See Fig. 1 to shown in Figure 3, a kind of full-automatic determining instrument of osmotic fragility comprises a base 1, and the base front end is furnished with two rightabout heparin tube conveyer mechanisms 2 and 3 for delivery of support of blood collecting pipe 9, its middle being provided with is inhaled sample position 4; Base postmedian both sides are provided with for delivery of the cuvette conveyer mechanism 5 of multi-joint cuvette 10 and useless cuvette and collect position 6, the centre is gear train bearing support 7, gear train bearing support 7 is provided with initial bit 11, sample absorbing device 8 is installed on the described gear train bearing support 7, described sample absorbing device 8 comprises a suction tube 17 and is used for the suction sample support piece 16 of its outer wall washing, the plane motion of form before and after this sample absorbing device can be done up and down by the control of stepper motor; The place of pressing close to inhale sample position 4 under the gear train bearing support 7 is provided for shining the photodetector that reactant liquor produces the permeability light signal.
The Main Function of gear train bearing support 7 is to carry sample absorbing device 8 to move.Suction tube 17 is preferably steel, and for ease of the puncture blood collecting pipe, its tip designs is circle inclined-plane piercing needle; For reducing to greatest extent the possibility of the sticking blood of outside surface, its outside surface is covered with the teflon anti-stick coating.Inhaling sample supports piece 16 shapes to be preferably rectangular parallelepiped, the center has vertical passageway 14, the internal diameter of described vertical passageway 14 is slightly larger than suction tube 17 external diameters, the side, position of its top about 20% has interconnection 15, described interconnection 15 links to each other with solenoid valve and peristaltic pump by pipeline, both can play the effect that keeps suction tube 17 vertical, and can introduce the outside surface that pure water clean suction tubes 17 by the interconnection 15 on it again.Suction tube 17 passes inhales the vertical passageway 14 that sample is supported piece 16, and the upper end links to each other with solenoid valve and peristaltic pump by rubber tubing, can realize inhaling the effect of cleaning in sample and the pipe.
Photodetector comprises for generation of the light source 12 of light signal and the receiver 13 of receiving optical signals, both relative spacings are slightly larger than the position of a cuvette external diameter, the pushing block of cuvette conveyer mechanism 5 is advanced into multi-joint cuvette 17 between described light source 12 and the described receiver 13 first during use, light source 12 starts after the reaction beginning, and received device 13 was collected after light signal passed cuvette light face.According to architectural feature and the experiment screening of human erythrocyte, the wavelength of light signal can be chosen to be 840～1200nm.
The heparin tube number that the ware number of multi-joint cuvette 10 and support of blood collecting pipe 9 arranges is corresponding, and the equal built-in an amount of NaCl-PBS damping fluid of each ware of multi-joint cuvette 10.
The present invention also comprises: be used for various stepper motors, sample presentation belt and the sample presentation pushing block of transmission cuvette, test tube rack and sample absorbing device, be used for the various solenoid valves of pipeline-cleaning, air blowing, the peristaltic pump of be used for suction, blowing, taking a sample.For guaranteeing test result reliability, corresponding outer cover also can be installed with the protection instrument.
The present invention also has a perfect control, data processing and analysis, demonstration, communication circuitry, be used for finishing the various stepper motors of control, solenoid valve, peristaltic pump, convert light signal to electric signal, amplify, be converted to again digital signal, carry out communicating by letter between Data Management Analysis, data demonstration, instrument and PC or the miscellaneous equipment.
Determination step of the present invention is as follows: heparin tube conveyer mechanism 2 is transported to suction sample position 4 by the support of blood collecting pipe 9 that pushing block will carry heparin tube, cuvette conveyer mechanism 5 is transported to the photodetector position with cuvette simultaneously, suction tube 17 moves to from initial bit 11 inhales sample position 4, from moving to again first ware top of multi-joint cuvette 10 after first heparin tube is inhaled sample, quantitative blood sample is injected cuvette, and air blowing mixing (hybrid mode is not limited to blow); The liquid turbidity that photodetector begins to detect in the cuvette changes, and microprocessor calculates the relative hemolysis rate in the setting-up time, and meanwhile suction tube 17 returns initial bit and cleans; After detect finishing, each moves the position of a unit to pushing block with multi-joint cuvette 10 and support of blood collecting pipe 9, begins next test; After whole row's heparin tube and 10 tests of multi-joint cuvette were finished, heparin tube conveyer mechanism 3 was taken support of blood collecting pipe 9 outputs away, and pushing block is pushed into useless cuvette with multi-joint cuvette 10 and collects position 6.
The present invention adopts light tight dynamic photoextinction to measure osmotic fragility, determine that through lot of experiments test parameter is as follows: with blood sample and pH7.4, concentration 2.5～5g/L NaCl-PBS damping fluid (preferred 3.5g/L NaCl-PBS damping fluid) is in the preferred 1:75 of 1:50～100() mixed the arriving in the 1cm optical path cuvette of ratio, with fill gas foam method formula rapid mixing, near infrared light (preferred 940nm wavelength) with wavelength 840～1200nm, measure behind the mixing first second and end second (is 30～60 seconds according to test situation, preferred the 40th second) time mixed liquor absorbance, the number percent of absorbance when calculating the first second drop-out value to end second absorbance with first second, can draw relative hemolysis rate value, be lower than critical value with relative hemolysis rate value, for fragility reduces; Be equal to or greater than critical value with relative hemolysis rate value, be fragility normal (also may be unusual for fragility if the hemolysis rate value is too high relatively).
The computing method of the relative hemolysis rate of the used light tight dynamic photoextinction of the present invention:
The below is further described the present invention and running thereof.
One, prepare: the support of blood collecting pipe 9 that will carry heparin tube is placed on the heparin tube conveyer mechanism 2, the multi-joint cuvette 10 of the built-in 3.5g/L NaCl-PBS of every lattice damping fluid 1.5mL is placed on the cuvette conveyer mechanism 5, press the starting switch on the instrument, instrument brings into operation.
Two, instrument resets: sample absorbing device 8, sample presentation pushing block, travelling belt, various pump and valve are realized initialization.
Three, send into blood sample and hypotonic salt solusion: heparin tube conveyer mechanism 2 is delivered to Kao Bichu with support of blood collecting pipe along the P direction and is stopped, send blood sample to enter pushing block support of blood collecting pipe is delivered to suction sample position (the 1st heparin tube just in time arrives and inhale sample position central authorities) along the M direction, cuvette conveyer mechanism 5 is delivered to Kao Bichu with the multi-joint cuvette 10 of the hypotonic salt solusion of splendid attire along the E direction and is stopped, send cuvette to enter pushing block cuvette is delivered to photodetector place (the 1st cuvette just in time arrives in the middle of the photodetector) along the L direction, sample absorbing device 8 runs to initial bit 11, opens peristaltic pump and solenoid valve outer wall and the inwall of suction tube 17 cleaned.
Four, detect: sample absorbing device 8 operations arrive inhales place, sample position, and suction tube 17 inserts downwards along the B direction and draws blood sample 20 μ l in first heparin tube, and then suction tube moves up along the A direction and arrives the position, top; Sample absorbing device 8 arrives place, photodetector top from inhaling 4 operations of sample position, this moment, first cuvette was in the middle of the photodetector, suction tube inserts in the cuvette downwards along the B direction, blood sample injected in the cuvette and turn on pump continues to blow to cuvette and made the reactant liquor mixing in two seconds, then suction tube moves up along the A direction and arrives the position, top, and photoelectric detective circuit begins to detect the variation of reactant liquor turbidity in the cuvette; Simultaneously sample absorbing device 8 moves and returns initial bit 11, opens peristaltic pump and solenoid valve outer wall and the inwall of suction tube 17 cleaned; Send blood sample to enter pushing block with the distance of support of blood collecting pipe 9 along a heparin tube of M direction propelling, send cuvette to enter pushing block with the distance of multi-joint cuvette 10 along a cuvette of L direction propelling, so repeatedly 7 blood samples are detected.
Five, detect to finish: send blood sample to enter pushing block and shift support of blood collecting pipe 9 onto end place along the M direction and stop, heparin tube conveyer mechanism 3 is exported support of blood collecting pipe 9 along the O direction, send cuvette to enter pushing block and shift multi-joint cuvette 10 onto end place along the L direction, multi-joint cuvette 10 will automatically slide into useless cuvette collection position 6 and collect, system calculates the relative hemolysis rate value of tested blood sample automatically, and display screen shows 7 blood examination results.
Above-described embodiment can not be thought limiting the scope of the invention; change or improvement that those skilled in the art make based on technical scheme of the present invention or means; simple transformation such as suction tube material or shape; the simple replacement of critical piece or position transposing; measure the simple change of the condition determinations such as wavelength, buffer concentration, detection time, all should fall within the protection domain of claim of the present invention.
Claims (10)
1. the full-automatic determining instrument of an osmotic fragility, it is characterized in that: comprise a base (1), the base front end is furnished with two rightabout heparin tube conveyer mechanisms (2,3) for delivery of support of blood collecting pipe (9), its middle being provided with is inhaled sample position (4); Base postmedian both sides are provided with for delivery of the cuvette conveyer mechanism (5) of multi-joint cuvette (10) and useless cuvette and collect position (6), the centre is gear train bearing support (7), gear train bearing support (7) is provided with initial bit (11), sample absorbing device (8) is installed on the described gear train bearing support (7), described sample absorbing device (8) comprises a suction tube (17) and is used for the suction sample support piece (16) of its outer wall washing, the plane motion of form before and after this sample absorbing device can be done up and down by the control of stepper motor; The place of pressing close to inhale sample position (4) under the gear train bearing support (7) is provided for shining the photodetector that reactant liquor produces the permeability light signal.
2. the full-automatic determining instrument of osmotic fragility as claimed in claim 1, it is characterized in that: described suction sample supports piece (16) to be shaped as rectangular parallelepiped, inhaling sample supports piece (16) center to have vertical passageway (14), the internal diameter of described vertical passageway (14) is greater than suction tube (17) external diameter, inhale sample and support the side, position on piece (16) top to have interconnection (15), described interconnection (15) links to each other with solenoid valve and peristaltic pump by pipeline.
3. the full-automatic determining instrument of osmotic fragility as claimed in claim 1, it is characterized in that: described suction tube (17) is firm matter, terminally is circle inclined-plane piercing needle.
4. the full-automatic determining instrument of osmotic fragility as claimed in claim 1, it is characterized in that: described suction tube (17) outside surface is covered with the teflon anti-stick coating.
5. the full-automatic determining instrument of osmotic fragility as claimed in claim 2, it is characterized in that: described suction tube (17) passes inhales the vertical passageway (14) that sample is supported piece (16), and suction tube (17) upper end links to each other with solenoid valve and peristaltic pump by pipeline.
6. the full-automatic determining instrument of osmotic fragility as claimed in claim 1, it is characterized in that: described photodetector comprises light source (12) for generation of light signal and the receiver (13) of receiving optical signals, both relative spacings are greater than the position of a cuvette external diameter, the pushing block of cuvette conveyer mechanism (5) pushes multi-joint cuvette (10) between described light source (12) and the described receiver (13) first during use, light source (12) starts after the reaction beginning, and received device was collected after light signal passed cuvette light face.
7. such as the full-automatic determining instrument of osmotic fragility as described in claim 1 or 6, it is characterized in that: the wavelength of described photodetector light signal is 840～1200nm.
8. assay method of the full-automatic determining instrument of osmotic fragility as claimed in claim 1, it is characterized in that: comprise following determination step: heparin tube conveyer mechanism (2) is transported to suction sample position (4) by the support of blood collecting pipe (9) that pushing block will carry heparin tube, cuvette conveyer mechanism (5) is transported to the photodetector position with cuvette simultaneously, suction tube (17) moves to from initial bit (11) inhales sample position (4), from moving to again first ware top of multi-joint cuvette (10) after first heparin tube is inhaled sample, quantitative blood sample is injected cuvette, and the air blowing mixing; The liquid turbidity that photodetector begins to detect in the cuvette changes, and microprocessor calculates the relative hemolysis rate in the setting-up time, and meanwhile suction tube (17) returns initial bit (11) and cleans; After detect finishing, each moves the position of a unit to pushing block with multi-joint cuvette (10) and support of blood collecting pipe (9), begins next test; After whole row's heparin tube and multi-joint cuvette (10) test were finished, heparin tube conveyer mechanism (3) was taken support of blood collecting pipe (9) output away, and pushing block is pushed into useless cuvette with multi-joint cuvette (10) and collects position (6).
9. the assay method of the full-automatic determining instrument of osmotic fragility as claimed in claim 8, it is characterized in that: the ware number of described multi-joint cuvette (10) is corresponding with the heparin tube number that support of blood collecting pipe (9) arranges.
10. the assay method of the full-automatic determining instrument of osmotic fragility as claimed in claim 8, it is characterized in that: each ware of described multi-joint cuvette (10) is built-in an amount of NaCl-PBS damping fluid all.
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| CN201210564999.3A CN103033482B (en) | 2012-12-24 | 2012-12-24 | Full-automatic determining instrument of red blood cell osmotic fragility |
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| CN201210564999.3A CN103033482B (en) | 2012-12-24 | 2012-12-24 | Full-automatic determining instrument of red blood cell osmotic fragility |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103344589A (en) * | 2013-07-05 | 2013-10-09 | 深圳奥萨制药有限公司 | Quasi-automatic in-vitro detection device and method for measuring HCY (homocysteine) by colorimetric method |
| CN103344638A (en) * | 2013-07-05 | 2013-10-09 | 深圳奥萨制药有限公司 | Semi-automatic in-vitro detection equipment and method for measuring HCY (Homocysteine) by adopting colorimetric method |
| CN104048920A (en) * | 2014-06-24 | 2014-09-17 | 深圳市锦瑞电子有限公司 | Turbidimeter and liquid analysis equipment |
| CN105928907A (en) * | 2016-05-17 | 2016-09-07 | 深圳普门科技有限公司 | Erythrocyte-osmotic-fragility measuring method based on scattering turbidimetric technology |
| CN107290294A (en) * | 2017-08-21 | 2017-10-24 | 杭州金域医学检验所有限公司 | Osmotic fragility detects screening technique and its application |
| CN107796776A (en) * | 2017-09-23 | 2018-03-13 | 南京律智诚专利技术开发有限公司 | A kind of ultraviolet spectrometer automatic sample handling system |
| CN109694810A (en) * | 2019-01-10 | 2019-04-30 | 祁光荣 | Full automatic serum separator |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103344589A (en) * | 2013-07-05 | 2013-10-09 | 深圳奥萨制药有限公司 | Quasi-automatic in-vitro detection device and method for measuring HCY (homocysteine) by colorimetric method |
| CN103344638A (en) * | 2013-07-05 | 2013-10-09 | 深圳奥萨制药有限公司 | Semi-automatic in-vitro detection equipment and method for measuring HCY (Homocysteine) by adopting colorimetric method |
| CN103344638B (en) * | 2013-07-05 | 2016-06-29 | 深圳奥萨制药有限公司 | A kind of semi-automatic external adopting colorimetry to measure HCY detection equipment |
| CN103344589B (en) * | 2013-07-05 | 2016-06-29 | 深圳奥萨制药有限公司 | A kind of accurate vitro detection equipment automatically adopting colorimetry to measure HCY |
| CN104048920A (en) * | 2014-06-24 | 2014-09-17 | 深圳市锦瑞电子有限公司 | Turbidimeter and liquid analysis equipment |
| CN104048920B (en) * | 2014-06-24 | 2016-09-07 | 深圳市锦瑞生物科技有限公司 | A kind of scopometer and fluid analysis equipment |
| CN105928907A (en) * | 2016-05-17 | 2016-09-07 | 深圳普门科技有限公司 | Erythrocyte-osmotic-fragility measuring method based on scattering turbidimetric technology |
| CN107290294A (en) * | 2017-08-21 | 2017-10-24 | 杭州金域医学检验所有限公司 | Osmotic fragility detects screening technique and its application |
| CN107796776A (en) * | 2017-09-23 | 2018-03-13 | 南京律智诚专利技术开发有限公司 | A kind of ultraviolet spectrometer automatic sample handling system |
| CN109694810A (en) * | 2019-01-10 | 2019-04-30 | 祁光荣 | Full automatic serum separator |
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