CN103027740A - Absorbable bone screw with slow-release biological factor and preparation method thereof - Google Patents
Absorbable bone screw with slow-release biological factor and preparation method thereof Download PDFInfo
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- CN103027740A CN103027740A CN2013100180156A CN201310018015A CN103027740A CN 103027740 A CN103027740 A CN 103027740A CN 2013100180156 A CN2013100180156 A CN 2013100180156A CN 201310018015 A CN201310018015 A CN 201310018015A CN 103027740 A CN103027740 A CN 103027740A
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- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 98
- 238000002360 preparation method Methods 0.000 title claims description 21
- 239000003181 biological factor Substances 0.000 title abstract 5
- 239000004005 microsphere Substances 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 16
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 32
- 239000002245 particle Substances 0.000 claims description 18
- 239000011780 sodium chloride Substances 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 14
- 230000017423 tissue regeneration Effects 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000000853 adhesive Substances 0.000 claims description 12
- 230000001070 adhesive effect Effects 0.000 claims description 12
- 239000006185 dispersion Substances 0.000 claims description 12
- 230000015572 biosynthetic process Effects 0.000 claims description 8
- 238000004945 emulsification Methods 0.000 claims description 8
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 claims description 7
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000001125 extrusion Methods 0.000 claims description 6
- 239000003960 organic solvent Substances 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 5
- 210000002435 tendon Anatomy 0.000 abstract description 4
- 210000000323 shoulder joint Anatomy 0.000 abstract description 2
- 102000007350 Bone Morphogenetic Proteins Human genes 0.000 abstract 1
- 108010007726 Bone Morphogenetic Proteins Proteins 0.000 abstract 1
- 229940112869 bone morphogenetic protein Drugs 0.000 abstract 1
- 239000008187 granular material Substances 0.000 description 12
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 8
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- 208000031481 Pathologic Constriction Diseases 0.000 description 3
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- 238000005213 imbibition Methods 0.000 description 2
- 229910052738 indium Inorganic materials 0.000 description 2
- 238000003760 magnetic stirring Methods 0.000 description 2
- 125000000896 monocarboxylic acid group Chemical group 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
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Abstract
The invention discloses an absorbable bone screw with a slow-release biological factor, wherein a porous structure of 1 micron to 50 microns is arranged in a thread bottom and/or a part that is 1/3 or less far away from a thread root on the thread; and the porous structure carries a slow-release biological factor rhBMP (Recombinant Human Bone Morphogenetic Protein)-2. The invention further discloses a method for preparing the absorbable bone screw with the slow-release biological factor. According to the invention, the difficulty that a tissue-regenerating biological factor loaded on the surface of the absorbable bone screw is easy to drop and lose is overcome successfully; and the practical value is strong. Absorbable bone screw-combined rhBMP-2 microspheres provided by the invention play roles of long-time slow release bone in-growth facilitation in the early stage, and can be widely used for fixing tendon interface screws and shoulder joint rivets.
Description
Technical field
The invention belongs to the composite material surface processing method that bone tissue restoration is used, particularly relate to a kind of absorbable bone screw with slow-release bio factor and preparation method thereof.
Background technology
Bone screw is used for fixing impaired osseous tissue or osseous tissue and tendon.Common bone screw is the metal bone screw, and it is present in the osseous tissue as ectosome, can only integrate by bone to be combined with osseous tissue, can't be replaced by physiological osseous tissue along with osteanagenesis.In recent years adopt that to have polylactic acid to be that the absorbed high molecular polymer of representative prepares the bone screw development very fast, its main feature is can be along with the regeneration of osseous tissue, and itself is absorbed bone screw gradually, and is finally replaced by new life's osseous tissue.Make up such as the high strength absorbable bone screw: 201120074842.3, can absorb bone screw: 200720005227.0.But in the absorbable bone screw absorption process, osteanagenesis speed is slower, easily causes the problems such as the bone defect repair is slow.
The Orthopedic Journal of China, 2 phases of calendar year 2001 disclose: rhBMP-2 (rhBMP-2) skeletonization research on mechanism and application present situation thereof during bone is repaired.Adopt Microspheres Technique, BMP is prepared into dosage form activity stabilized, long-time slow release.Yet rhBMP-is made in the existing report of slow releasing agent, the envelop rate of slow releasing agent is low, is generally 84%, and its slow release effect does not reach patient's pathology demand yet.In addition, can introduce tissue regeneration active factors layer by coating way on the bone screw surface, but because the friction in the screw screw-in process, tissue regeneration active factors coating is easy to come off, and is difficult to play regularly the effect that localization discharges.
Summary of the invention
In order to solve the absorbable bone screw area load tissue regeneration biotic factor difficult problem of loss that easily comes off, but the object of the present invention is to provide the bone screw of the load slow release tissue regeneration active factors on biological absorbable bone screw surface.
Another object of the present invention is to provide a kind of preparation method for preparing above-mentioned bone screw.
In order to realize purpose of the present invention, technical scheme of the present invention is as follows:
A kind of absorbable bone screw with slow-release bio factor, comprise screw rod, be provided with screw thread in the screw rod periphery, described screw thread comprises at the bottom of ridge and the tooth, wherein, being provided with 1um ~ 50um cavernous structure apart from ridge root 1/3rd following positions on the described Bottom of Thread and/or on the described ridge, be loaded with slow-release bio factor rhBMP-2 in the described cavernous structure.
Preferably, described rhBMP-2 is that granularity is 1um, and envelop rate is the microsphere greater than 98.86%.
Further, the invention provides the above-mentioned method with absorbable bone screw of the slow-release bio factor of preparation, may further comprise the steps:
(1) the preparation table mask has the bone screw of 1um ~ 50um cavernous structure;
(2) prepared sizes are 1um, and envelop rate is the rhBMP-2 microsphere greater than 98.86%;
(3) the rhBMP-2 microsphere with step (2) gained pours in the bone screw of step (1) gained.
Wherein, step (1) preparation table mask has the bone screw of 1um ~ 50um cavernous structure, specifically may further comprise the steps:
1a) sodium chloride particle is ground screening, obtains required corresponding with hole dimension, granularity at 1 micron 50 micron particle, and be dispersed in the organic solvent dioxane formation dispersion liquid A in the ratio of 50 ~ 200g/L;
1b) bone screw is immersed in step 1a) among the gained dispersion liquid A, lift out solution with the speed of 10 ~ 30 cm per minute, and oven dry;
1c) with step 1b) the gained bone screw deposits in 130 ~ 180 ℃ baking oven after 2 ~ 20 minutes and to take out, with consistent with the Bottom of Thread shape, and Breadth Maximum is not more than the metallic roll of ridge 1/3rd At The Height width along Bottom of Thread and the roll extrusion of screw thread pressure tooth root;
1d) blow away the not bulged-in sodium chloride particle in bone screw surface with compressed air, bone screw is immersed remove sodium chloride particle in the deionized water solution afterwards, namely get the bone screw that the tissue regeneration active factors can be carried in described surface after the oven dry.
Wherein, described (2) prepared sizes are 1 μ m, and envelop rate is greater than 98.86% rhBMP-2 microsphere, and is preferred, adopts the ultrasonic multi-emulsion method of W/O/W (W/O/W).
Described ultrasonic multi-emulsion method refers to the ultrasonic multi-emulsion method that list of references Diederik etc. mentions in the Retention of in vitro and in vivo BMP-2 bioactivities in sustained delivery vehicles for bone tissue engineering that Biomaterials 29 (2008) 3245 ~ 3252 delivers.
Concrete, ultrasonic multi-emulsion method preparation process is as follows among the present invention:
2a) 50 μ g rhBMP-2 add 150 μ l PBS liquid (PH7.40.01), imbibition rifle sucking-off 1 μ l places in the 1mL glass centrifuge tube, wait until ELISA and measure (wherein containing rhBMP-20.33 μ g), 400mg PLGA (molecular weight 10,000,75/25 end is COOH) is dissolved in 1mL CH
2CL
2In, as oil phase; With liquid-transfering gun interior water is added in the oil phase, ultrasonic 30 seconds, form uniformly W/O one-level breast of white, immediately it is sucked with No. 16 syringe needles of 1mL syringe, changing No. 9 syringe needles adds to 60mL and stirred among the 2%PVA (ice bath) 1 minute under high speed homogenizer 5600rpm condition, formation secondary breast, magnetic stirring apparatus low speed volatilization 3h.Centrifugal, leave and take supernatant (totally 4 supernatant).
2b) in vacuum desiccator, add first anhydrous P
2O
5As desiccant, evacuation is placed on 4 ℃ of refrigerators, gets the rhBMP-2 microsphere of homogeneous powder powder.
The present invention adopts the ultrasonic multi-emulsion method of W/O/W (W/O/W), its rotating speed is 5600rpm, prepares granularity at 1 micron left and right sides rhBMP-2 microsphere, and previous literature report rhBMP-2 particle diameter is the 20-30 micron, 1 micron microsphere of the present invention preparation is more suitable for the coating of nail porous surface.Select glass container (preventing that plastics are to the absorption of albumen), omnidistance ice bath in the preparation process of the present invention, adjust suitable rotating speed etc., adopt enzyme connection sandwich assay, record envelop rate up to 98.86%, all high than the previous literature report.
Wherein, step (3) also comprises in the rhBMP-2 microsphere of gained and adds medical adhesive; Preferably, the mass volume ratio of described rhBMP-2 microsphere and described medical adhesive is 5:1mg/mL.Wherein said medical adhesive is the Kang Paite medical adhesive, utilizes the good flowability of this medical adhesive, in proportion the rhBMP-2 microsphere is added in this medical adhesive, is prepared into suspension, and utilizes its good flowability, this suspension is poured in the micropore of nail.Have loose structure and very strong adhesion after the Kang Paite medical adhesive that the present invention uses becomes glue, it can be controlled the prominent of rhBMP-2 microsphere and release, and makes release about 20% in the rhBMP-2 microsphere 8 hours, and total slow-release time reaches 1 month.
Beneficial effect of the present invention is as follows:
The present invention utilizes the sodium chloride particle plunging to comprise below 1/3rd that under the absorbable bone screw thread depth surface preparation of the bottom of thread makes loose structure, the method is simple, convenient, the sodium chloride of its use to nail without any negative effect, the loose structure of gained is even, hole is little, can carry and slow release tissue regeneration active factors.Simultaneously, because pore structure is in the bottom of thread, lessly in screw screw-in process, produce friction with tissue, can effectively introduce the tissue regeneration active factors, carry out slow release.In addition, screw thread top still keeps original smooth state, is easy to the screw-in of bone screw.
In addition, rhBMP-2 microspherulite diameter of the present invention is little only to be about 1 micron, and envelop rate is more suitable for the coating of nail porous surface up to more than 98.86%.The present invention uses prominent the releasing of Kang Paite medical adhesive control rhBMP-2 microsphere, discharges about 20% in 8 hours, and reaches long-time slow release, approximately 1 month, makes absorbable bone screw of the present invention play better repair to fixing osseous tissue or tendon.
In a word, the present invention has successfully solved the difficult problem that absorbable bone screw area load tissue regeneration biotic factor easily comes off and loses, and has very strong practical value.The compound rhBMP-2 microsphere of absorbable bone screw provided by the invention has long-time slow release, and the effect that the early promotion bone is grown into can be widely used in fixedly interference screw, the shoulder joint rivet of tendon.
Description of drawings
Fig. 1 is the screw thread partial cutaway schematic (wherein h represents the height of ridge, and d is the micropore position from ridge root 1/3rd following positions) of bone screw of the present invention.
Number in the figure is as follows:
Ridge 1, at the bottom of the tooth 2, cavernous structure 3, ridge root 11, ridge top 12.
The specific embodiment
The below is clearly and completely described the technical scheme in the embodiment of the invention, and obviously, described embodiment only is the present invention's part embodiment, rather than whole embodiment.Based on the embodiment among the present invention, those of ordinary skills belong to the scope of protection of the invention not making the every other embodiment that obtains under the creative work prerequisite.
The medicine that arrives involved in the present invention such as dioxane etc., is commercial the purchase; Used instrument such as Ultrasound Instrument, baking oven, liquid-transfering gun, homogenizer etc., is the normal experiment instrument that buys on the market; Test involved method, such as grinding, stirring, czochralski method etc., if all be the conventional usings method of those skilled in the art without specified otherwise.
Embodiment 1
(1) the preparation table mask has the bone screw of 10um cavernous structure;
1a) sodium chloride particle is ground screening, obtains required corresponding with hole dimension, the granule of particle mean size about 10 microns, and get 25g sodium chloride and join in the 500mL organic solvent dioxane dispersion liquid A of formation 50g/L;
1b) bone screw is immersed in step 1a) among the gained dispersion liquid A, lift out solution with the speed of 10 cm per minute, and oven dry;
1c) with step 1b) the gained bone screw deposits in 130 ℃ baking oven after 10 minutes and to take out, and with consistent with Bottom of Thread 2 shapes, and Breadth Maximum is not more than the metallic roll of ridge 1/1st three At The Height width along Bottom of Thread 2 and 11 roll extrusion of ridge root; (as shown in Figure 1)
1d) blow away the not bulged-in sodium oxide granule in bone screw surface with compressed air, afterwards bone screw is immersed deionized water and dissolve the sodium oxide granule, namely get described surface apertures after the oven dry in 10 microns left and right sides cavernous structures 3 (as shown in Figure 1) and the bone screw that can carry the tissue regeneration active factors.
(2) prepared sizes are 1um, and envelop rate is the rhBMP-2 microsphere greater than 98.86%;
2a) 50 μ g rhBMP-2 add 150 μ l PBS liquid (PH7.4 0.01), imbibition rifle sucking-off 1 μ l places in the 1mL glass centrifuge tube, wait until ELISA and measure (wherein containing rhBMP-2 0.33 μ g), 400mgPLGA (molecular weight 10,000,75/25 end is COOH) is dissolved in 1mL CH
2CL
2In, as oil phase; With liquid-transfering gun interior water is added in the oil phase, ultrasonic 30 seconds, form uniformly W/O one-level breast of white, immediately it is sucked with No. 16 syringe needles of 1mL syringe, changing No. 9 syringe needles adds to 60mL and stirred among the 2%PVA (ice bath) 1 minute under high speed homogenizer 5600rpm condition, formation secondary breast, magnetic stirring apparatus low speed volatilization 3h.Centrifugal, leave and take supernatant (totally 4 supernatant).
2b) in vacuum desiccator, add first anhydrous P
2O
5As desiccant, evacuation is placed on 4 ℃ of refrigerators, gets the rhBMP-2 microsphere of homogeneous powder powder.
The ultrasonic multi-emulsion method of W/O/W (W/O/W) that the present invention adopts, its rotating speed is 5600rpm, prepares granularity at 1 micron left and right sides rhBMP-2 microsphere.Select glass container (preventing that plastics are to the absorption of albumen), omnidistance ice bath in the preparation process of the present invention, adjust suitable rotating speed etc., adopt enzyme connection sandwich assay, record envelop rate up to 98.86%.
(3) the rhBMP-2 microsphere with step (2) gained pours in the bone screw of step (1) gained.
Utilize the good flowability of Kang Paite medical adhesive, get 5mg and adopt the rhBMP-2 microsphere of above-mentioned preparation to add in the 1ml medical adhesive, be prepared into suspension, utilize its good flowability, pour in the micropore of nail.
The bone screw that the present embodiment obtains, as shown in Figure 1, it comprises screw rod, be provided with screw thread in the screw rod periphery, described screw thread comprises at the bottom of ridge 1 and the tooth 2, wherein, being provided with 10um cavernous structure 3 apart from ridge root 1/3rd following positions on the described Bottom of Thread 2 and/or on the described ridge 1, be loaded with slow-release bio factor rhBMP-2 in the described cavernous structure.Experiment showed, the bone screw of this compound rhBMP-2, discharge about 20% in 8 hours, and slow-release time can reach long 1 month.
Embodiment 2
(1) the preparation table mask has the bone screw of 20um cavernous structure;
1a) sodium chloride particle is ground screening, obtains required corresponding with hole dimension, the granule of particle mean size about 20 microns, and get 25g sodium chloride and join in the 300mL organic solvent dioxane dispersion liquid A of formation 83.3g/L;
1b) bone screw is immersed in step 1a) among the gained dispersion liquid A, lift out solution with the speed of 15 cm per minute, and oven dry;
1c) with step 1b) the gained bone screw deposits in 150 ℃ baking oven after 15 minutes and to take out, and with consistent with bottom of thread shape, and Breadth Maximum is not more than the metallic roll of screw thread 1/3rd At The Height width along stricture of vagina bottom roll extrusion.
1d) blow away the not bulged-in sodium oxide granule in bone screw surface with compressed air, afterwards bone screw is immersed deionized water and dissolve the sodium oxide granule, namely get described surface apertures after the oven dry in 20 microns left and right sides cavernous structures 3 (as shown in Figure 1) and the bone screw that can carry the tissue regeneration active factors.
(2) prepared sizes are 1um, and envelop rate is the rhBMP-2 microsphere greater than 98.86%;
(3) the rhBMP-2 microsphere with step (2) gained pours in the bone screw of step (1) gained.
Step (2), (3) are with embodiment 1.
The bone screw that the present embodiment obtains, as shown in Figure 1, it comprises screw rod, be provided with screw thread in the screw rod periphery, described screw thread comprises at the bottom of ridge 1 and the tooth 2, wherein, being provided with 20um cavernous structure 3 apart from ridge root 1/3rd following positions on the described Bottom of Thread 2 and/or on the described ridge 1, be loaded with slow-release bio factor rhBMP-2 in the described cavernous structure.Experiment showed, the bone screw of this compound rhBMP-2, discharge about 20% in 8 hours, and slow-release time can reach long 1 month.
(1) the preparation table mask has the bone screw of 2um cavernous structure;
1a) sodium chloride particle is ground screening, obtains required corresponding with hole dimension, the granule of particle mean size about 2 microns, and get 20g sodium chloride and join in the 200mL organic solvent dioxane dispersion liquid A of formation 100g/L;
1b) bone screw is immersed in step 1a) among the gained dispersion liquid A, lift out solution with the speed of 20 cm per minute, and oven dry;
1c) with step 1b) the gained bone screw deposits in 140 ℃ baking oven after 8 minutes and to take out, and with consistent with bottom of thread shape, and Breadth Maximum is not more than the metallic roll of screw thread 1/3rd At The Height width along stricture of vagina bottom roll extrusion.
1d) blow away the not bulged-in sodium oxide granule in bone screw surface with compressed air, afterwards bone screw is immersed deionized water and dissolve the sodium oxide granule, namely get described surface apertures after the oven dry in 2 microns left and right sides cavernous structures 3 (as shown in Figure 1) and the bone screw that can carry the tissue regeneration active factors.
(2) prepared sizes are 1um, and envelop rate is the rhBMP-2 microsphere greater than 98.86%;
(3) the rhBMP-2 microsphere with step (2) gained pours in the bone screw of step (1) gained.
Step (2), (3) are with embodiment 1.
The bone screw that the present embodiment obtains, as shown in Figure 1, it comprises screw rod, be provided with screw thread in the screw rod periphery, described screw thread comprises at the bottom of ridge 1 and the tooth 2, wherein, being provided with 2um cavernous structure 3 apart from ridge root 1/3rd following positions on the described Bottom of Thread 2 and/or on the described ridge 1, be loaded with slow-release bio factor rhBMP-2 in the described cavernous structure.Experiment showed, the bone screw of this compound rhBMP-2, discharge about 20% in 8 hours, and slow-release time can reach long 1 month.
Embodiment 4
(1) the preparation table mask has the bone screw of 35um cavernous structure;
1a) sodium chloride particle is ground screening, obtains required corresponding with hole dimension, the granule of particle mean size about 35 microns, and get 40g sodium chloride and join in the 500mL organic solvent dioxane dispersion liquid A of formation 80g/L;
1b) bone screw is immersed in step 1a) among the gained dispersion liquid A, lift out solution with the speed of 30 cm per minute, and oven dry;
1c) with step 1b) the gained bone screw deposits in 180 ℃ baking oven after 5 minutes and to take out, and with consistent with bottom of thread shape, and Breadth Maximum is not more than the metallic roll of screw thread 1/3rd At The Height width along stricture of vagina bottom roll extrusion.
1d) blow away the not bulged-in sodium oxide granule in bone screw surface with compressed air, afterwards bone screw is immersed deionized water and dissolve the sodium oxide granule, namely get described surface apertures after the oven dry in 35 microns left and right sides cavernous structures 3 (as shown in Figure 1) and the bone screw that can carry the tissue regeneration active factors.
(2) prepared sizes are 1um, and envelop rate is the rhBMP-2 microsphere greater than 98.86%;
(3) the rhBMP-2 microsphere with step (2) gained pours in the bone screw of step (1) gained.
Step (2), (3) are with embodiment 1.
The bone screw that the present embodiment obtains, as shown in Figure 1, it comprises screw rod, be provided with screw thread in the screw rod periphery, described screw thread comprises at the bottom of ridge 1 and the tooth 2, wherein, being provided with 35um cavernous structure 3 apart from ridge root 1/3rd following positions on the described Bottom of Thread 2 and/or on the described ridge 1, be loaded with slow-release bio factor rhBMP-2 in the described cavernous structure.Experiment showed, the bone screw of this compound rhBMP-2, discharge about 20% in 8 hours, and slow-release time can reach long 1 month.
To the above-mentioned explanation of the disclosed embodiments, make this area professional and technical personnel can realize or use the present invention.Multiple modification to these embodiment will be apparent concerning those skilled in the art, and General Principle as defined herein can be in the situation that do not break away from the spirit or scope of the present invention, in other embodiments realization.Therefore, the present invention will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (8)
1. absorbable bone screw with slow-release bio factor, comprise screw rod, be provided with screw thread in the screw rod periphery, described screw thread comprises at the bottom of ridge and the tooth, it is characterized in that, being provided with 1um ~ 50um cavernous structure apart from ridge root 1/3rd following positions on the described Bottom of Thread and/or on the described ridge, be loaded with slow-release bio factor rhBMP-2 in the described cavernous structure.
2. bone screw according to claim 1 is characterized in that, described rhBMP-2 is that granularity is 1um, and envelop rate is the microsphere greater than 98.86%.
3. one kind prepares claim 1 or 2 described methods with absorbable bone screw of the slow-release bio factor, it is characterized in that, may further comprise the steps:
(1) the preparation table mask has the bone screw of 1um ~ 50um cavernous structure;
(2) prepared sizes are 1um, and envelop rate is the rhBMP-2 microsphere greater than 98.86%;
(3) the rhBMP-2 microsphere with step (2) gained pours in the bone screw of step (1) gained.
4. described method according to claim 3 is characterized in that, step (1) preparation table mask has the bone screw of 1um ~ 50um cavernous structure, specifically may further comprise the steps:
1a) sodium chloride particle is ground screening, obtains required corresponding with hole dimension, granularity at 1 micron 50 micron particle, and be dispersed in the organic solvent dioxane formation dispersion liquid A in the ratio of 50 ~ 200g/L;
1b) bone screw is immersed in step 1a) among the gained dispersion liquid A, lift out solution with the speed of 10 ~ 30 cm per minute, and oven dry;
1c) with step 1b) the gained bone screw deposits in 130 ~ 180 ℃ baking oven after 2 ~ 20 minutes and to take out, with consistent with the Bottom of Thread shape, and Breadth Maximum is not more than the metallic roll of ridge 1/3rd At The Height width along Bottom of Thread and the roll extrusion of screw thread pressure tooth root;
1d) blow away the not bulged-in sodium chloride particle in bone screw surface with compressed air, bone screw is immersed remove sodium chloride particle in the deionized water solution afterwards, namely get the bone screw that the tissue regeneration active factors can be carried in described surface after the oven dry.
5. described method according to claim 3 is characterized in that, preparation method described in the step (2) adopts the ultrasonic multi-emulsion method of W/O/W (W/O/W).
6. described method according to claim 5 is characterized in that, selecting glass container, omnidistance ice bath, adjustment rotating speed in the preparation process of described ultrasonic multi-emulsion method is 5600rpm.
7. described method according to claim 3 is characterized in that, step (3) also comprises in the rhBMP-2 microsphere of gained and adds medical adhesive.
8. described method according to claim 7 is characterized in that, the mass volume ratio of described rhBMP-2 microsphere and described medical adhesive is 5:1mg/mL.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN111616787A (en) * | 2020-05-23 | 2020-09-04 | 徐州长风生物科技有限公司 | Prestress anti-back bone screw assembly |
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| CN103027740B (en) | 2014-12-24 |
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