CN103014124B - Staphylococcus lugdunensis selective medium and preparation method thereof - Google Patents
Staphylococcus lugdunensis selective medium and preparation method thereof Download PDFInfo
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- CN103014124B CN103014124B CN 201310010739 CN201310010739A CN103014124B CN 103014124 B CN103014124 B CN 103014124B CN 201310010739 CN201310010739 CN 201310010739 CN 201310010739 A CN201310010739 A CN 201310010739A CN 103014124 B CN103014124 B CN 103014124B
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- selective medium
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- coetsoidin
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Abstract
The invention discloses a staphylococcus lugdunensis selective medium and a preparation method thereof. The staphylococcus lugdunensis selective medium is characterized in that the formula for preparing1000 ml selective medium contains the following materials: 15 g of malt extract powder, 150 ml of egg white liquid, 5 g of sodium chloride, 0.5 g of magnesium citrate, 15 g of agar, 8-12 mg of coetsoidin A, 0.03 g of 6-O-acetyldaidzin, 0.2 g of potassium dihydrogen phosphate and the balance being distilled water made up to 1000 ml. According to the preparation method based on an optimal formula, the malt extract powder, egg white, sodium chloride, magnesium citrate, agar, coetsoidin A, 6-O-acetyldaidzin, potassium dihydrogen phosphate, L-cysteine and copper sulfate are dissolved in a yam water decoction liquid, mixed uniformly to achieve constant volume, sterilized and packaged. Compared with the prior art, the staphylococcus lugdunensis selective medium and the preparation method thereof have the characteristics of being reliable to inspect and high in separation rate of staphylococcus lugdunensis.
Description
Technical field
The present invention relates to field of medical examination, be specifically related to a kind of road Deng staphylococcus selective medium and preparation method thereof.
Background technology
Road Deng staphylococcus equals reported first in 1988 by Freney.In recent years, the report of the invasive infection that causes of road Deng staphylococcus increases increasingly.It causes scholar's extensive concern as the pathogenic bacterium with remarkable clinical microbiology feature, more has the scholar to be referred to as Surreptitious Staphylococcus or " draping over one's shoulders the wolf of sheepskin ".Road Deng staphylococcus and various clinical disease are closely related, comprise abscess, endocarditis, peritonitis, urinary tract infection, central nervous system infection, bone joint infection etc.
Because road Deng staphylococcus is the normal microflora of human body skin, the halfway words of blood sampling operation sterilization, road Deng staphylococcus is easy to sneak in the blood, causes blood contamination and spreads disease.So efficient, reliably road Deng staphylococcus cultivation is for the basis of studying, identifying this bacterial classification.
Because previously road Deng staphylococcus often is used as the cultivation contaminated bacteria, therefore still lacks at present and be used for the substratum that road Deng staphylococcus selectivity is cultivated.
Summary of the invention
Technical assignment of the present invention is for above the deficiencies in the prior art, provides a kind of Deng of satisfying the need staphylococcus separation rate high selective medium.
The technical scheme that the present invention solves its technical problem is: a kind of road Deng staphylococcus selective medium, and its feature exists, and contains in the prescription of preparation 1000ml selective medium:
Fructus Hordei Germinatus soaks powder 15g;
Chicken Albumin liquid 150ml;
Sodium-chlor 5g;
Citrate of magnesia 0.5g;
Agar 15g;
Coetsoidin A 8 ~ 12mg;
6-O-acetyl daidzin 0.03g;
Potassium primary phosphate 0.2g;
Distilled water adds to 1000ml.
In the prioritization scheme, every 1000ml selective medium also contains copper sulfate 6mg.
In the prioritization scheme, every 1000ml selective medium also contains Cys 0.1g.
In the prioritization scheme, every 1000ml selective medium also contains Chinese yam water cooking liquid 200 ~ 300ml of concentration 200g/L.
In the optimization scheme, every 1000ml selective medium also contains copper sulfate 6mg; Cys 0.1g; Chinese yam water cooking liquid 200 ~ 300ml of concentration 200g/L.
The preparation method of a kind of road Deng staphylococcus selective medium is characterized in that may further comprise the steps:
(1) get Chinese yam and added water boil 1 hour, filter, get filtrate, adjusting volume is 200g/L to concentration, gets the Chinese yam water cooking liquid;
(2) get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; 6-O-acetyl daidzin; Potassium primary phosphate; Cys and copper sulfate are dissolved in the step 1 gained Chinese yam water cooking liquid, after mixing filters, and distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after the sterilization.
Wherein said Fructus Hordei Germinatus soaks powder, Chicken Albumin liquid provides carbon nitrogen source and energy; Citrate of magnesia and potassium primary phosphate are buffer reagent, and can suppress varied bacteria growing; Cys belongs to amino acids, is nutrition agent; Agar is the peptizer of selective medium; Coetsoidin A has special bacteriostatic action for golden Portugal bacterium, and is invalid for road Deng staphylococcus; Chicken Albumin liquid contains each peptide species, fat, somatomedin, hormone etc., and these materials are to promoting that the staphylococcic Growth and Reproduction of road Deng all is very important factor, and Chicken Albumin liquid also has the constant effect of selective medium pH value of keeping in addition.6-O-acetyl daidzin is for the staphylococcic specificity nutrition agent of road Deng, can specific increase road Deng staphylococcus reproduction speed.Cupric ion belongs to heavy metal ion, under this concentration dose of 6mg/L, it is inner that cupric ion is deposited on golden Portugal bacterium, causes cell wall to disappear or attenuation, and rrna is gathered into agglomerate, finally cause bacterium distortion and break, present the property damage of heavy metal source, and road Deng staphylococcus is lower than golden Portugal bacterium to transportcapacity and the penetrating power of heavy metal, also is that the resistance of heavy metal is higher, even this concentration of 6mg/L also can tolerate, can effectively suppress golden Portugal bacteria growing so add copper sulfate.Chinese yam is for being the dry rhizome of Dioscoreaceae plant Chinese yam Dioscorea opposita Thunb., contain the Multiple components such as protein, fat, starch, also first sugar, amino acid, energy metabolism, cell development, the metabolic factor can be provided, especially the Chinese yam stem tuber contains diosgenin (diosgenin) and polyphenoloxidase, help lend some impetus to road Deng's aureus growth, the especially growth of bacterium colony under the heavy metal environment.
The present invention has reliable, the high characteristics of road Deng staphylococcus separation rate of detecting compared with prior art.
Embodiment
Below in conjunction with practical situation, the specific embodiment of the present invention is elaborated.
Embodiment 1, and contain in the prescription of preparation 1000ml selective medium: Fructus Hordei Germinatus soaks powder 15g; Chicken Albumin liquid 150ml; Sodium-chlor 5g; Citrate of magnesia 0.5g; Agar 15g; Coetsoidin A 8mg; 6-O-acetyl daidzin 0.03g; Potassium primary phosphate
0.2G; Distilled water adds to 1000ml.Preparation method: get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; 6-O-acetyl daidzin; Potassium primary phosphate is dissolved in the distilled water, after mixing filters, and distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after the sterilization.
Embodiment 2, and contain in the prescription of preparation 1000ml selective medium: Fructus Hordei Germinatus soaks powder 15g; Chicken Albumin liquid 150ml; Sodium-chlor 5g; Citrate of magnesia 0.5g; Agar 15g; Coetsoidin A 12mg; 6-O-acetyl daidzin 0.03g; Potassium primary phosphate
0.2G; Copper sulfate 6mg; Distilled water adds to 1000ml.Preparation method: get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; 6-O-acetyl daidzin; Potassium primary phosphate; Copper sulfate is dissolved in the distilled water, after mixing filters, and distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after the sterilization.
Embodiment 3, and contain in the prescription of preparation 1000ml selective medium: Fructus Hordei Germinatus soaks powder 15g; Chicken Albumin liquid 150ml; Sodium-chlor 5g; Citrate of magnesia 0.5g; Agar 15g; Coetsoidin A 10mg; 6-O-acetyl daidzin 0.03g; Potassium primary phosphate
0.2G; Cys
0.1G; Distilled water adds to 1000ml.Preparation method: get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; 6-O-acetyl daidzin; Potassium primary phosphate; Cys is dissolved in the distilled water, after mixing filters, and distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after the sterilization.
Embodiment 4, and contain in the prescription of preparation 1000ml selective medium: Fructus Hordei Germinatus soaks powder 15g; Chicken Albumin liquid 150ml; Sodium-chlor 5g; Citrate of magnesia 0.5g; Agar 15g; Coetsoidin A 9mg; 6-O-acetyl daidzin 0.03g; Potassium primary phosphate
0.2G; The Chinese yam water cooking liquid 200ml of concentration 200g/L; Distilled water adds to 1000ml.The preparation method:
(1) get Chinese yam and added water boil 1 hour, filter, get filtrate, adjusting volume is 200g/L to concentration, gets the Chinese yam water cooking liquid;
(2) get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; 6-O-acetyl daidzin; Potassium primary phosphate; Be dissolved in the step 1 gained Chinese yam water cooking liquid, after mixing filters, distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after the sterilization.
Embodiment 5, and contain in the prescription of preparation 1000ml selective medium: Fructus Hordei Germinatus soaks powder 15g; Chicken Albumin liquid 150ml; Sodium-chlor 5g; Citrate of magnesia 0.5g; Agar 15g; Coetsoidin A 11mg; 6-O-acetyl daidzin 0.03g; Potassium primary phosphate
0.2G; Copper sulfate 6mg; Cys
0.1G; The Chinese yam water cooking liquid 300ml of concentration 200g/L; Distilled water adds to 1000ml.The preparation method is:
(1) get Chinese yam and added water boil 1 hour, filter, get filtrate, adjusting volume is 200g/L to concentration, gets the Chinese yam water cooking liquid;
(2) get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; 6-O-acetyl daidzin; Potassium primary phosphate; Cys and copper sulfate are dissolved in the step 1 gained Chinese yam water cooking liquid, after mixing filters, and distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after the sterilization.
Described Chinese yam water cooking liquid concentration is 200g/L, refers in every liter of Chinese yam water cooking liquid and contains crude drug 200g.
Gained of the present invention road Deng staphylococcus selective medium has and detects reliable, the high characteristics of road Deng staphylococcus separation rate, is the clinical data sufficient proof, and pertinent data is as follows.
1 object and method.
1.1 selective medium preparation: establish altogether 3 groups, be respectively control group, embodiment 1 scheme group, embodiment 5 scheme groups.Control group is staphylococcus selective agar 110(CHAPMAN agar), filling a prescription is: pancreas casein peptone, yeast soak powder, gelatin, PEARLITOL 25C, lactose, sodium-chlor, dipotassium hydrogen phosphate, agar.Prescription and the preparation method of embodiment 1 scheme group, embodiment 5 scheme groups see embodiment.
1.2 method: with sneaking into gold-coloured staphylococci in 40 parts of samples of determining road Deng staphylococcus positive via PCR, be inoculated on the substratum, under 37 ℃, hatched 24 hours.
1.3 statistical analysis: carry out statistical study with SPSS 13.0, P＜0.05 expression has significant.
2 results: in 40 parts of inoculation samples, microbial culture is separated and to be accredited as road Deng staphylococcic, and embodiment 1 scheme group is 32 examples, and embodiment 5 scheme groups are 36 examples.Control group CHAPMAN nutrient agar bacterium colony is golden Portugal bacterium through identifying to be proved at last, learns by statistics and processes, and various embodiments of the present invention group and control group relatively have notable difference (P＜0.001).The result shows, embodiment of the invention road Deng staphylococcus separation rate is apparently higher than existing selective medium.
Claims (1)
1. road Deng staphylococcus selective medium, its feature exists, and contains in the prescription of preparation 1000ml selective medium:
Fructus Hordei Germinatus soaks powder 15g;
Chicken Albumin liquid 150ml;
Sodium-chlor 5g;
Citrate of magnesia 0.5g;
Agar 15g;
Coetsoidin A 8 ~ 12mg;
6-O-acetyl daidzin 0.03g;
Potassium primary phosphate 0.2g;
Distilled water adds to 1000ml.
2A kind of road according to claim 1 Deng staphylococcus selective medium, its feature exists: every 1000ml selective medium also contains copper sulfate 6mg; Cys 0.1g; Chinese yam water cooking liquid 200 ~ 300ml of concentration 200g/L.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101566622A (en) * | 2008-04-21 | 2009-10-28 | 于维森 | Fast check paper of Staphylococcus aureus causing food poisoning |
| CN102286611A (en) * | 2011-07-22 | 2011-12-21 | 卢家镇 | Staphylococcus list identification method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| TWI415572B (en) * | 2011-06-17 | 2013-11-21 | Univ Chang Gung | Inhibition of biofilm formation by 1,2,3,4,6-penta-o-galloyl-d-glucopyranose |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101566622A (en) * | 2008-04-21 | 2009-10-28 | 于维森 | Fast check paper of Staphylococcus aureus causing food poisoning |
| CN102286611A (en) * | 2011-07-22 | 2011-12-21 | 卢家镇 | Staphylococcus list identification method |
Non-Patent Citations (3)
| Title |
|---|
| 王沛等.荧光原位杂交快速鉴定金黄色葡萄球菌和凝固酶阴性葡萄球菌.《中华检验医学杂志》.2004,第27卷(第7期),434-436. |
| 王茜，吴超.路邓葡萄球菌感染的研究进展.《中华医院感染学杂志》.2010,第20卷(第15期),2358-2360. * |
| 荧光原位杂交快速鉴定金黄色葡萄球菌和凝固酶阴性葡萄球菌;王沛等;《中华检验医学杂志》;20040730;第27卷(第7期);434-436 * |
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