CN103014082B - Biological preparation method of (R)-4-cyano-hydroxybutanoate - Google Patents
Biological preparation method of (R)-4-cyano-hydroxybutanoate Download PDFInfo
- Publication number
- CN103014082B CN103014082B CN201210556375.7A CN201210556375A CN103014082B CN 103014082 B CN103014082 B CN 103014082B CN 201210556375 A CN201210556375 A CN 201210556375A CN 103014082 B CN103014082 B CN 103014082B
- Authority
- CN
- China
- Prior art keywords
- ethyl butyrate
- cyano
- substrate
- reaction
- add
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention relates to a biological preparation method of (R)-4-cyano-hydroxybutanoate. According to the biological preparation method, (S)-4-chlorine-3-hydroxybutanoate is used as a substrate; under the action of a biocatalyst, the substrate reacts with sodium cyanide to generate a target product (R)-4-cyano-hydroxybutanoate; the biocatalyst is recombination halohydrin dehalogenase; and the reaction is carried out in a water phase with the pH of 6 to 8. According to the invention, by adopting the recombination halohydrin dehalogenase, the concentration of the substrate is improved and the enzyme dosage is reduced, so that production cost is reduced. Moreover, by optimizing the process, both purity and yield of the product are improved.
Description
Technical field
The invention belongs to bio-pharmaceuticals and Green Chemistry field, be specifically related to the biological preparation method of one (R)-4-cyano-3-hydroxy ethyl butyrate
Background technology
Atorvastatin (atorvastatin, trade(brand)name Lipitor, Lipitor) is that first annual sales amount exceedes the medicine of 10,000,000,000 dollars in the world.It is by suppressing hydroxymethyl glutaryl CoA reductase enzyme, and the rate-limiting step that hinders cholesterol biosynthesis in liver reduces the content of Blood Cholesterol.
The committed step of technique is to utilize synthetic (the R)-4-cyano-3-hydroxy ethyl butyrate (A5) (Angew.Chem.Int.Ed.2005,44:362-365) of (S)-4-chloro-3-hydroxyl ethyl butyrate (A4) single stage method now.
In this step reaction, under the alkaline condition of having avoided existing in chemical method due to biological process, form by product too much, the shortcoming of separation and purification difficulty, therefore utilizes halohydrin dehalogenase (halohydrin dehalogenase, HHDH) studied from the technique of the synthetic A5 of A4.US Patent No. 7125693 and US 7132267 have announced the research of Codexis company for this project, 1.5% (w/w) that its enzyme charging capacity is substrate, and thick yield is 93%.Domestic patent CN 102168117A improves this technique afterwards, has realized the recycling of sodium cyanide, but its enzyme charging capacity up to 8.0% (w/w) of substrate, thick yield is only 83.8%.
The defects such as comprehensive above literature search information, in the technique that we find to exist at present, exists enzyme dosage too high, and reaction conditions is inappropriate.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of biological preparation method of improved (R)-4-cyano-3-hydroxy ethyl butyrate.
For solving above technical problem, the present invention adopts following technical scheme:
A kind of biological preparation method of (R)-4-cyano-3-hydroxy ethyl butyrate, it is taking (S)-4-chloro-3-hydroxyl ethyl butyrate as substrate, this substrate is under the effect of biological catalyst, react with sodium cyanide and generate target product (R)-4-cyano-3-hydroxy ethyl butyrate, described biological catalyst is restructuring halohydrin dehalogenase, the preparation method of described restructuring halohydrin dehalogenase is: by halohydrin dehalogenase list colony inoculation to containing in the liquid LB substratum of amicillin resistance, shaking table activation 8-12 hour at 30-40 DEG C, the culture obtaining after activation is transferred in the liquid LB substratum containing amicillin resistance, at 30-40 DEG C, enlarged culturing to OD600 value reaches 0.6-0.8, add inductor, continue to cultivate 8-12 hour in 25-35 DEG C, centrifugal collecting precipitate, add phosphate buffered saline buffer to obtain suspension, suspension is placed in to ice bath ultrasonic disruption 8-12 minute, centrifugal again, separation of supernatant, it is-10 ~-25 DEG C by supernatant liquor pre-freeze to temperature, and then freeze-drying 24-48 hour, obtain restructuring halohydrin dehalogenase, in the water that described reaction is 6-8 at pH, carry out.
Further, in reaction system when initial, the concentration of described substrate (S)-4-chloro-3-hydroxyl ethyl butyrate is 150-200mg/ml, and described restructuring halohydrin dehalogenase is the 0.4-1%(w/w of substrate (S)-4-chloro-3-hydroxyl ethyl butyrate).
Further, in the reaction system when initial, the mol ratio of described sodium cyanide and described substrate (S)-4-chloro-3-hydroxyl ethyl butyrate is 1.5-2.5:1.
Further, the transformation efficiency that reaction finishes rear substrate is 98-100%, and product optical purity is greater than 99%, and purity is greater than 97%.
Preferably, in the water that described reaction is 7.0 at pH, carry out.
Preferably, the restructuring halohydrin dehalogenase described in described reaction is the 0.5%(w/w of substrate (S)-4-chloro-3-hydroxyl ethyl butyrate).
Preferably, in described reaction, the concentration of substrate (S)-4-chloro-3-hydroxyl ethyl butyrate is 200mg/ml.
Preferably, described inductor is isopropyl-β-D-thiogalactoside(IPTG) or lactose.
Further, described preparation method's implementation process is as follows: in reaction vessel, add successively water, substrate (S)-4-chloro-3-hydroxyl ethyl butyrate, after stirring, slowly drip sodium cyanide solution, add again restructuring halohydrin dehalogenase, stirring reaction at 40-60 DEG C, utilize GC detection reaction process, when rate to be transformed is greater than 98%, add sulphur acid for adjusting pH to 2-3, add ethyl acetate extraction, merge organic phase, rotary evaporation obtains (R)-4-cyano-3-hydroxy ethyl butyrate product.
According to the present invention, except restructuring halohydrin dehalogenase, all the other raw materials all can be commercially available by general channel.
Beneficial effect of the present invention is:
The present invention recombinates halohydrin dehalogenase catalyzed reaction also by the optimization to technique by employing, and concentration of substrate is improved, and enzyme dosage reduces, thereby has reduced production cost, and the purity of product and yield are all increased.
Embodiment
Reaction formula of the present invention is as follows:
The following examples can make the present invention of professional and technical personnel's comprehend, but do not limit the present invention in any way.
Embodiment mono-
From glycerine pipe or transform dull and stereotyped single colony inoculation to 4mL containing the liquid LB substratum activation of amicillin resistance spend the night (37 DEG C, 200rpm).From overnight culture with 1/100(v/v) inoculum size switching 100mL is containing the liquid LB substratum of amicillin resistance, 37 DEG C, 200rpm shaking culture to OD600 value reach 0.6-0.8, add IPTG in 30 DEG C of continuation overnight incubation.Centrifugal collecting cell, with 10mL phosphoric acid buffer (2mM, pH7.0) suspension cell.Cell suspending liquid is placed in ice bath ultrasonic disruption 10 minutes.Centrifugal, supernatant liquor pre-freeze is spent the night.Freeze-drying 24h-48h.
Embodiment bis-
In 50mL there-necked flask, add successively deionized water 23mL, substrate 7g, after stirring, slowly splash in 30% NaCN solution 5mL(dropping process with 20% sulphuric acid soln maintenance system pH 7.2～7.3), halohydrin dehalogenase powder 35mg, in 50 ° of C, pH 7.0(30%NaCN solution titration), under 800rpm magnetic agitation condition, reaction 24h, GC detects transformation efficiency >98%, add sulphur acid for adjusting pH to 2-3, add equal-volume ethyl acetate, diatomite filtration, collect organic phase, water adds equal-volume ethyl acetate extracting twice, merge organic phase, rotary evaporation obtains product 6.3g, purity >98%, optical purity >99%.
Embodiment tri-
In reactor, add successively deionized water 2.3L, substrate 700g, after stirring, slowly splash in 30% NaCN solution 0.5L(dropping process with 20% sulphuric acid soln maintenance system pH 7.2～7.3), halohydrin dehalogenase powder 3.5g, in 50 ° of C, pH 7.0(30%NaCN solution titration), under mechanical stirring condition, reaction 24h, GC detects transformation efficiency >98%, add sulphur acid for adjusting pH to 2-3, add equal-volume ethyl acetate, diatomite filtration, collect organic phase, water adds equal-volume ethyl acetate extracting twice, merge organic phase, pass into nitrogen by alkali lye to slough HCN gas, rotary evaporation obtains product 630g left and right, purity >98%, optical purity >99%.
Above-described embodiment is only explanation technical conceive of the present invention and feature, and its object is to allow person skilled in the art can understand content of the present invention and implement according to this, can not limit the scope of the invention with this.All equivalences that spirit is done according to the present invention change or modify, within all should being encompassed in protection scope of the present invention.
Claims (5)
1. the biological preparation method of (R)-4-cyano-3-hydroxy ethyl butyrate, it is taking (S)-4-chloro-3-hydroxyl ethyl butyrate as substrate, this substrate is under the effect of biological catalyst, react with sodium cyanide and generate target product (R)-4-cyano-3-hydroxy ethyl butyrate, it is characterized in that: described biological catalyst is restructuring halohydrin dehalogenase, the preparation method of described restructuring halohydrin dehalogenase is: by halohydrin dehalogenase list colony inoculation to containing in the liquid LB substratum of amicillin resistance, shaking table activation 8-12 hour at 30-40 DEG C, the culture obtaining after activation is transferred in the liquid LB substratum containing amicillin resistance, at 30-40 DEG C, enlarged culturing to OD600 value reaches 0.6-0.8, add inductor, continue to cultivate 8-12 hour in 25-35 DEG C, centrifugal collecting precipitate, add phosphate buffered saline buffer to obtain suspension, suspension is placed in to ice bath ultrasonic disruption 8-12 minute, centrifugal again, separation of supernatant, it is-10 ~-25 DEG C by supernatant liquor pre-freeze to temperature, and then freeze-drying 24-48 hour, obtain restructuring halohydrin dehalogenase, in the water that described reaction is 6-8 at pH, carry out, in reaction system when initial, the concentration of described substrate (S)-4-chloro-3-hydroxyl ethyl butyrate is 150-200 mg/ml, and described restructuring halohydrin dehalogenase is the 0.4-1%(w/w of substrate (S)-4-chloro-3-hydroxyl ethyl butyrate).
2. the biological preparation method of (R)-4-cyano-3-hydroxy ethyl butyrate according to claim 1, it is characterized in that: in the reaction system when initial, the mol ratio of described sodium cyanide and described substrate (S)-4-chloro-3-hydroxyl ethyl butyrate is 1.5-2.5:1.
3. the biological preparation method of (R)-4-cyano-3-hydroxy ethyl butyrate according to claim 1, is characterized in that: in the water that described reaction is 7.0 at pH, carry out.
4. the biological preparation method of (R)-4-cyano-3-hydroxy ethyl butyrate according to claim 1, is characterized in that: described inductor is isopropyl-β-D-thiogalactoside(IPTG) or lactose.
5. according to the biological preparation method of (the R)-4-cyano-3-hydroxy ethyl butyrate described in any one in claim 1-2, it is characterized in that: described preparation method's implementation process is as follows: in reaction vessel, add successively water, substrate (S)-4-chloro-3-hydroxyl ethyl butyrate, after stirring, slowly drip sodium cyanide solution, add again restructuring halohydrin dehalogenase, stirring reaction at 40-60 DEG C, utilize GC detection reaction process, when rate to be transformed is greater than 98%, add sulphur acid for adjusting pH to 2-3, add ethyl acetate extraction, merge organic phase, rotary evaporation obtains (R)-4-cyano-3-hydroxy ethyl butyrate product.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210556375.7A CN103014082B (en) | 2012-12-20 | 2012-12-20 | Biological preparation method of (R)-4-cyano-hydroxybutanoate |
| PCT/CN2013/077813 WO2014075447A1 (en) | 2012-11-19 | 2013-06-24 | Biological preparation method of ethyl (r)-4-cyano-hydroxybutanoate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210556375.7A CN103014082B (en) | 2012-12-20 | 2012-12-20 | Biological preparation method of (R)-4-cyano-hydroxybutanoate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103014082A CN103014082A (en) | 2013-04-03 |
| CN103014082B true CN103014082B (en) | 2014-08-27 |
Family
ID=47963197
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210556375.7A Active CN103014082B (en) | 2012-11-19 | 2012-12-20 | Biological preparation method of (R)-4-cyano-hydroxybutanoate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103014082B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014075447A1 (en) * | 2012-11-19 | 2014-05-22 | 苏州汉酶生物技术有限公司 | Biological preparation method of ethyl (r)-4-cyano-hydroxybutanoate |
| CN104372038A (en) * | 2013-08-12 | 2015-02-25 | 南京朗恩生物科技有限公司 | Two-step catalytic preparation method of (R)-4-cyan-3-hydroxyvinyl butyrate |
| CN105567655B (en) * | 2014-10-14 | 2020-11-20 | 上海弈柯莱生物医药科技有限公司 | Halogen alcohol dehalogenase and application thereof in synthesis of statin drug intermediate |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7132267B2 (en) * | 2002-08-09 | 2006-11-07 | Codexis, Inc. | Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives and vicinal cyano, hydroxy substituted carboxylic acid esters |
-
2012
- 2012-12-20 CN CN201210556375.7A patent/CN103014082B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7132267B2 (en) * | 2002-08-09 | 2006-11-07 | Codexis, Inc. | Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives and vicinal cyano, hydroxy substituted carboxylic acid esters |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103014082A (en) | 2013-04-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103014081A (en) | Biological preparation method of 3-amino-4-(2,4,5-trifluorophenyl)methyl butyrate | |
| CN103014082B (en) | Biological preparation method of (R)-4-cyano-hydroxybutanoate | |
| CN102605011B (en) | (S)-4-chloride-3-ethyl 3-hydroxybutyrate biological preparation method | |
| CN110003151B (en) | Process for the preparation of furanonic acids | |
| CN102925501A (en) | Biological preparation method of (S)-4-chloro-3-hydroxybutyrate ethyl | |
| CN104830922A (en) | Enzymatic conversion method of L-ornithine | |
| CN102827912A (en) | Technology for preparing medicine intermediate D-7-ACA by two enzyme carriers one-step method | |
| CN103695379B (en) | Restructuring ketoreductase and use it to prepare the method for oxiracetam intermediate | |
| WO2014094462A1 (en) | Biological preparation method of (s)-3-methylamino-1-(2-thienyl)-1-propyl alcohol | |
| CN102286602A (en) | Method for resolving DL-arginine by microbial enzyme method for preparing D-arginine hydrochloride and L-ornithine hydrochloride | |
| CN102234313A (en) | Method for synthesizing pidotimod | |
| CN101560532A (en) | L-theanine enzymatic transformation preparation method | |
| CN105349583A (en) | Method for preparing (R)-o-chloromandelic acid through enzyme and application of enzyme | |
| CN104372038A (en) | Two-step catalytic preparation method of (R)-4-cyan-3-hydroxyvinyl butyrate | |
| CN110257448B (en) | Method for converting arginine into agmatine by utilizing thallus whole cell catalysis | |
| CN104072369B (en) | A kind of technique preparing Diisopropyl malonate | |
| CN101886096B (en) | Method for preparing 2-amino-2, 3-dimethyl butamide by microbial catalysis method and strain | |
| CN112852656B (en) | Escherichia coli engineering bacterium for producing glycollic acid by using glucose and acetic acid dual-carbon source | |
| CN104263772B (en) | A kind of method that acetyl phosphate is prepared based on biology enzyme | |
| CN101863801A (en) | Preparation method of optical activity citrulline or homocitrulline | |
| CN101864370A (en) | Yeast strain converting quinuclidone into R-3-quinuclidinol and conversion method thereof | |
| CN101348808B (en) | Double enzyme coupling preparation of ornithine hydrochloride | |
| CN104178540A (en) | Method for synthesizing ademetionine by biological catalytic process | |
| CN104529838A (en) | Synthetic method of haloxyfop intermediate | |
| CN102226208B (en) | Preparation method of D-asparagine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |