CN103012071A - Method for extracting and purifying resveratrol from peanut meal - Google Patents
Method for extracting and purifying resveratrol from peanut meal Download PDFInfo
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- CN103012071A CN103012071A CN 201210512622 CN201210512622A CN103012071A CN 103012071 A CN103012071 A CN 103012071A CN 201210512622 CN201210512622 CN 201210512622 CN 201210512622 A CN201210512622 A CN 201210512622A CN 103012071 A CN103012071 A CN 103012071A
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Abstract
The invention relates to a method for extracting and purifying resveratrol from peanut meal. The peanut meal resources are abundant and easier to obtain, and the extracting and purifying method is simple. The method for extracting and purifying the resveratrol from the peanut meal provided by the invention comprises the following operation steps of: crushing and refining the peanut meal; adding ethanol solvent to the crushed and refined peanut meal for soaking, and carrying out ultrasonic wave extraction; carrying out vacuum filtration on the extracting solution to obtain crude extracting solution; mixing the filter residue with the ethanol solvent, repeatedly extracting the mixture, combining the crude extracting solution; and using polyamide resin to purify the combined crude extruding solution, and drying the purified crude extruding solution to obtain the resveratrol. The highest purity of the resveratrol produced by the invention can reach 98.7%.
Description
Technical field
The present invention relates to a kind of method of from peanut meal, extracting purified resveratrol.
Background technology
Trans-resveratrol has the preventive and therapeutic effect that suppresses tumour, anti-oxidant, Green Tea Extract, antithrombotic, antianaphylaxis, atherosclerosis and have coronary heart disease, ischemic heart disease, hyperlipidemia.Mainly be present in the plants such as giant knotweed, grape, peanut, pine tree.Trans-resveratrol has been listed in one of anti-cardiovascular, anticancer the most promising medicine.Trans-resveratrol is widely used in the world at present, has caused the significantly increase of trans-resveratrol demand.
Peanut meal be the peanut that shells be raw material, the byproduct after oil plant is refined in squeezing, multiplex fish and the cattle food done effectively do not utilize the trans-resveratrol that contains in the peanut meal, caused the significant wastage of resource.
Extraction process complexity and the extraction yield of current trans-resveratrol are low, and can use acetone and the virose reagent of methyl alcohol tool in the purge process, not only bring harm to operator, also cause in the extract objectionable impurities residual; Use the concentrated purification of vacuum-drying can not guarantee higher trans-resveratrol purity behind the wash-out.
Summary of the invention
For solving above technical problem, the invention provides a kind of method of from peanut meal, extracting purified resveratrol, the method is easy, extraction yield is high, environmental protection, production cost is low and can obtain highly purified trans-resveratrol.
Purpose of the present invention is achieved through the following technical solutions: a kind of method of extracting purified resveratrol from peanut meal may further comprise the steps:
(1) peanut meal dispersion and fining, 95% ethanol of 10 times of weight of adding soaked 2 ~ 4 hours;
(2) after the immersion mixture is carried out ultrasonic extraction;
(3) extracting solution is carried out vacuum filtration, gets crude extract, further filter residue is mixed with 75% ~ 95% alcohol solvent again, repeat to extract 2 times after, merge crude extract and concentrate;
(4) with polyamide resin on the concentrated extracting solution, use 25% alcohol solvent removal of impurities; Use 75%-95% alcohol solvent wash-out; Use the tlc tracking monitor in the process, collect and be rich in the merging of trans-resveratrol section elutriant; With the concentrated coarse crystallization that obtains of elutriant; With coarse crystallization ethyl alcohol recrystallization 4 ~ 5 times, vacuum lyophilization obtains trans-resveratrol.
Ultrasonic extraction described in the above-mentioned steps (2), its ultrasonic frequency are that 2750MHz, power are 300W ~ 400W, and extraction time is 10 minutes.
Concentrated condition described in the above-mentioned steps (3) is that rotary evaporation in vacuo is concentrated.
The invention has the beneficial effects as follows:
(1) to have adopted peanut meal be that raw material extracts trans-resveratrol in the present invention, and the method for utilizing the ultrasonic wave Assisted Ethanol to extract is extracted from peanut meal and isolated trans-resveratrol, has greatly improved the comprehensive utilization value of peanut meal.
(2) the present invention is by polyamide resin, and ethanol elution comes purified resveratrol, avoided the application of the toxic agent such as acetone, methyl alcohol, and the trans-resveratrol highest purity that obtains can reach 98.7%.
Embodiment
Embodiment 1
To get the 1Kg peanut meal, dispersion and fining, 95% ethanol of 10 times of weight of adding soaked 2 hours, and then mixture carries out ultrasonic extraction, and its ultrasonic frequency is that 2750MHz, power are 400W, and extraction time is 10 minutes.Extracting solution is carried out vacuum filtration, gets crude extract, further filter residue is mixed with 75% alcohol solvent again, repeat to extract 2 times after, merging crude extract, to carry out rotary evaporation in vacuo concentrated.With polyamide resin on the concentrated extracting solution, use 25% alcohol solvent removal of impurities, use 75% alcohol solvent wash-out; Use the tlc tracking monitor in the process, collect and be rich in the merging of trans-resveratrol section elutriant; With the concentrated coarse crystallization that obtains of elutriant; With coarse crystallization ethyl alcohol recrystallization 5 times, vacuum lyophilization obtains the 22.6g trans-resveratrol, and detecting its content through HPLC is 97.9%.
Embodiment 2
To get the 1Kg peanut meal, dispersion and fining, 95% ethanol of 10 times of weight of adding soaked 3 hours, and then mixture carries out ultrasonic extraction, and its ultrasonic frequency is that 2750MHz, power are 350W, and extraction time is 10 minutes.Extracting solution is carried out vacuum filtration, gets crude extract, further filter residue is mixed with 85% alcohol solvent again, repeat to extract 2 times after, merging crude extract, to carry out rotary evaporation in vacuo concentrated.With polyamide resin on the concentrated extracting solution, use 25% alcohol solvent removal of impurities, use 85% alcohol solvent wash-out; Use the tlc tracking monitor in the process, collect and be rich in the merging of trans-resveratrol section elutriant; With the concentrated coarse crystallization that obtains of elutriant; With coarse crystallization ethyl alcohol recrystallization 4 times, vacuum lyophilization obtains the 21.1g trans-resveratrol, and detecting its content through HPLC is 98.7%.
Embodiment 3
To get the 1Kg peanut meal, dispersion and fining, 95% ethanol of 10 times of weight of adding soaked 4 hours, and then mixture carries out ultrasonic extraction, and its ultrasonic frequency is that 2750MHz, power are 300W, and extraction time is 10 minutes.Extracting solution is carried out vacuum filtration, gets crude extract, further filter residue is mixed with 95% alcohol solvent again, repeat to extract 2 times after, merging crude extract, to carry out rotary evaporation in vacuo concentrated.With polyamide resin on the concentrated extracting solution, use 25% alcohol solvent removal of impurities, use 95% alcohol solvent wash-out; Use the tlc tracking monitor in the process, collect and be rich in the merging of trans-resveratrol section elutriant; With the concentrated coarse crystallization that obtains of elutriant; With coarse crystallization ethyl alcohol recrystallization 4 times, vacuum lyophilization obtains the 23.6g trans-resveratrol, and detecting its content through HPLC is 96.5%.
Above-described embodiment is not as a limitation of the invention, all any modifications of doing within the scope of the invention, is equal to replacement, improvement etc., all belongs to protection scope of the present invention.
Claims (3)
1. a method of extracting the purifying resveratrol from peanut meal is characterized in that, adopts following working method:
(1) peanut meal dispersion and fining, 95% ethanol of 10 times of weight of adding soaked 2 ~ 4 hours;
(2) after the immersion mixture is carried out ultrasonic extraction;
(3) extracting solution is carried out vacuum filtration, gets crude extract, further filter residue is mixed with 75% ~ 95% alcohol solvent again, repeat to extract 2 times after, merge crude extract and concentrate;
(4) with polyamide resin on the concentrated extracting solution, use 25% alcohol solvent removal of impurities; Use 75%-95% alcohol solvent wash-out; Use the tlc tracking monitor in the process, collect and be rich in the merging of trans-resveratrol section elutriant; With the concentrated coarse crystallization that obtains of elutriant; With coarse crystallization ethyl alcohol recrystallization 4 ~ 5 times, vacuum lyophilization obtains trans-resveratrol.
2. the method for extracting the purifying resveratrol from peanut meal according to claim 1, it is characterized in that: the ultrasonic extraction described in the step (2), its ultrasonic frequency are that 2750MHz, power are 300W ~ 400W, and extraction time is 10 minutes.
3. the method for extracting the purifying resveratrol from peanut meal according to claim 1, it is characterized in that: the concentrated condition described in the step (3) is that rotary evaporation in vacuo is concentrated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210512622 CN103012071A (en) | 2012-12-05 | 2012-12-05 | Method for extracting and purifying resveratrol from peanut meal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210512622 CN103012071A (en) | 2012-12-05 | 2012-12-05 | Method for extracting and purifying resveratrol from peanut meal |
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| CN103012071A true CN103012071A (en) | 2013-04-03 |
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| CN 201210512622 Pending CN103012071A (en) | 2012-12-05 | 2012-12-05 | Method for extracting and purifying resveratrol from peanut meal |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766913A (en) * | 2014-01-10 | 2014-05-07 | 河南科技大学 | Method for extracting total stilbene compound from penoy seeds |
| CN104163754A (en) * | 2014-07-18 | 2014-11-26 | 苏州市职业大学 | Method for extraction and separating of high purity resveratrol from peanut root |
| CN111454242A (en) * | 2020-05-14 | 2020-07-28 | 湖南华诚生物资源股份有限公司 | Method for separating multiple active ingredients from peanut coat |
-
2012
- 2012-12-05 CN CN 201210512622 patent/CN103012071A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766913A (en) * | 2014-01-10 | 2014-05-07 | 河南科技大学 | Method for extracting total stilbene compound from penoy seeds |
| CN103766913B (en) * | 2014-01-10 | 2016-08-17 | 河南科技大学 | A kind of method extracting total stilbene compound from seed of Flos Moutan |
| CN104163754A (en) * | 2014-07-18 | 2014-11-26 | 苏州市职业大学 | Method for extraction and separating of high purity resveratrol from peanut root |
| CN111454242A (en) * | 2020-05-14 | 2020-07-28 | 湖南华诚生物资源股份有限公司 | Method for separating multiple active ingredients from peanut coat |
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Application publication date: 20130403 |