CN102998437A - Sheath fluid used for urinary sediment analysis and preparation method thereof - Google Patents
Sheath fluid used for urinary sediment analysis and preparation method thereof Download PDFInfo
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- CN102998437A CN102998437A CN2012104860309A CN201210486030A CN102998437A CN 102998437 A CN102998437 A CN 102998437A CN 2012104860309 A CN2012104860309 A CN 2012104860309A CN 201210486030 A CN201210486030 A CN 201210486030A CN 102998437 A CN102998437 A CN 102998437A
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- sheath fluid
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Abstract
The invention discloses a sheath fluid used for urinary sediment analysis and a preparation method thereof. The sheath fluid comprises mother liquor as well as sodium chloride and glycerol substances which are dissolved in the mother liquor, wherein a buffering agent, a surfactant and a chelating agent are dissolved in the mother liquor, pH value of the mother liquor is 7-8; the mother liquor also contains a mould inhibitor and an antibacterial agent; the glycerol substances comprise glycerol and polyglycerol, wherein working concentration of the glycerol is 50-60g/L preferably; and the surfactant is a polyoxyethylene ether type surfactant and sorbitol polyoxyethylene ether preferably, and the working concentration of the surfactant is 100-2000mg/L and 300mg/L preferably. The sheath fluid disclosed by the invention is relatively stable in properties, and precipitates can be difficultly produced; the sheath fluid is applicable to an import instrument, and using effect of a domestic instrument is also considered, so that application range is wide and price is relatively lower; and corrosion to a metal part of the instrument is less, so that usage cost is greatly reduced.
Description
Technical field
The present invention relates to a kind of reagent and preparation method thereof, be specifically related to a kind of urine sediment analysis sheath fluid and preparation method thereof.
Background technology
At present, the urine sediments analyzer that uses in the hospital is divided into import instrument and domestic equipment, and import instrument is expensive, and supporting sheath fluid is also relatively expensive; Domestic equipment is relatively cheap, but the result of use of its sheath fluid and not fully up to expectations.No matter import sheath fluid or domestic sheath fluid in the use procedure, are separated out always have solid matter in the quantitative section of instrument, and these precipitates just very likely stop up sheath stream reacting part in case flow to sheath flow pool with the liquid road; In addition, when instrument does not use within a certain period of time, the sheath fluid evaporation in the sheath liquid device, device is inner just might to produce sediment, so also can stop up sheath stream reacting part.When above both of these case occurring, just need disassembling apparatus, remove tamper.And the pipeline that contacts with sheath fluid in the instrument must use corrosion-resistant material, also will regularly replace, and this has increased use cost greatly.
Summary of the invention
For solving the deficiencies in the prior art, the object of the present invention is to provide relatively stable, the difficult generation precipitation of a kind of character, instrument is corroded the lighter sheath fluid that is applicable to arena, and the method for preparation.
In order to realize above-mentioned target, the present invention adopts following technical scheme:
A kind of urine sediment analysis sheath fluid is characterized in that, comprising:
Mother liquor,
Be dissolved in sodium chloride and the glycerols material of aforementioned mother liquor;
Be dissolved with in the aforementioned mother liquor: buffering agent, surfactant, sequestrant, the pH value of mother liquor is 7-8.
Aforesaid urine sediment analysis sheath fluid is characterized in that, also comprises in the aforementioned mother liquor: mildewproof agent and antiseptic, preferred GXL and pyrithione.
Aforesaid urine sediment analysis sheath fluid is characterized in that, aforementioned glycerols material comprises: glycerine and polyglycereol, and preferably glycerine, the preferred working concentration of glycerine is 50-60g/L.
Aforesaid urine sediment analysis sheath fluid is characterized in that, aforementioned surfactants is Polyoxyethylene elher surfactant.
Aforesaid urine sediment analysis sheath fluid is characterized in that, aforementioned Polyoxyethylene elher surfactant is sorbitol polyoxyethylene ether, and working concentration is 100-2000mg/L, preferred 300 mg/L.
Aforesaid urine sediment analysis sheath fluid is characterized in that the working concentration of aforementioned sodium chloride is less than 16g/L.
Aforesaid urine sediment analysis sheath fluid is characterized in that, also comprises: sodium sulphate, working concentration is less than 14g/L.
Prepare aforesaid urine sediment analysis with the method for sheath fluid, it is characterized in that, may further comprise the steps:
(1), buffering agent, surfactant, sequestrant are dissolved in the deionized water, make the pH value and be the mother liquor of 7-8;
(2), sodium chloride is dissolved in the aforementioned mother liquor, obtain intermediate solution;
(3), the glycerols material is dissolved in the aforementioned intermediate solution, obtain sheath fluid.
The aforesaid urine sediment analysis for preparing is characterized in that with the method for sheath fluid, in step (1), continues to add mildewproof agent and antiseptic in aforementioned mother liquor.
The aforesaid urine sediment analysis for preparing is characterized in that with the method for sheath fluid, in step (2), continues to add sodium sulphate in intermediate solution.
Usefulness of the present invention is: sheath fluid character of the present invention is relatively stable, is difficult for producing precipitation; Be not only applicable to import instrument, and taken into account the result of use of domestic equipment, the scope of application is large, and price is relatively low; Metal partial corrosion to instrument is lighter, greatly reduces use cost.
Embodiment
Specifically introduce reagent of the present invention and preparation method thereof below in conjunction with specific embodiment.
At first, according to the ratio shown in the table 1, prepare three minutes mother liquors.
Table 1 mother liquor proportioning
| ? | Trishydroxymethylaminomethane | Hydrochloric acid 5N | Sorbitol polyoxyethylene ether | EDTAK 2 | GXL | Pyrithione |
| Mother liquor one | 1.5g | 3ml | 0.3g | 0.3g | 0.2g | 0.3g |
| Mother liquor two | 1.5g | 3ml | 1.0g | 0.3g | 0.2g | 0.3g |
| Mother liquor three | 1.5g | 3ml | 2.0g | 0.3g | 0.2g | 0.3g |
Compound method: after above-mentioned each component is ready to, be dissolved in the 1L deionized water, adjust the pH value of solution in the 7-8 scope, namely obtain mother liquor.
Need to prove, 1. the effect of buffering agent is that pH with sheath fluid is stabilized between the 7-8, so the kind to buffering agent has no particular limits, both can be that biological buffer also can be the inorganic salts buffering agent, both can use separately also and can be used in combination, in the present invention, preferred trishydroxymethylaminomethane-hydrochloric acid and trishydroxymethylaminomethane-maleic acid, and only describe take trishydroxymethylaminomethane-hydrochloric acid as representative, other buffers repeats no more; 2. in mother liquor, add surfactant, it mainly is the bubble that produces when sheath fluid flows in instrument in order to eliminate, so the kind of Surfactant is also without any restriction, as long as can suppress Bubble formation, it can be cationic surfactant, anionic surfactant, non-ionic surfactant, it also can be amphoteric surfactant, in the present invention, preferred Polyoxyethylene elher surfactant, sorbitol polyoxyethylene ether type for example, the APES type, the NPE type, the AEO type, because their character is very similar, so only describe take sorbitol polyoxyethylene ether as representative, other surfactants repeat no more; 3. complexing agent mainly is to promote complex compound to generate, thus to its kind also without any restriction, consumption is moderate to be got final product, the present invention is only with EDTAK
2For representative describes, other complexing agents repeat no more; 4. antiseptic and mildewproof agent are as auxiliary additive, it mainly is to play the generation of anti-bacteria and mould and the effect of breeding, so its kind is unrestricted, consumption is moderate to be got final product, the present invention only describes take GXL and pyrithione as representative, and other antiseptics and mildewproof agent repeat no more.
Next, according to the ratio shown in the table 2, the preparation sheath fluid.
Table 2 sheath fluid proportioning
| ? | Mother liquor one | Mother liquor two | Mother liquor three | Sodium chloride | Sodium sulphate | Glycerine | Three polyglycereol |
| Embodiment 1 | 1L | —— | —— | 3g | —— | 60g | —— |
| Embodiment 2 | —— | 1L | —— | 3g | —— | 60g | —— |
| Embodiment 3 | —— | —— | 1L | 3g | —— | 60g | —— |
| Embodiment 4 | 1L | —— | —— | 6.5g | —— | 56g | —— |
| Embodiment 5 | 1L | —— | —— | 16g | —— | 56g | —— |
| Embodiment 6 | 1L | —— | —— | —— | 6.5g | 56g | —— |
| Embodiment 7 | 1L | —— | —— | 6.5g | 13.5g | 56g | —— |
| Embodiment 8 | 1L | —— | —— | 6.5g | 13.5g | —— | 56g |
Compound method: in mother liquor, add sodium chloride or/and sodium sulphate obtains intermediate solution; In intermediate solution, continue to add glycerine or three polyglycereol again, mix and to obtain sheath fluid.
Measure the conductivity of sheath fluid and the amount of precipitate
(1), measure the sheath fluid conductivity: the DDSJ-318 type electric conductivity instrument that utilizes Hangzhou thunder magnetic experimental apparatus factory to produce is measured the mS/m of unit;
(2), measure the amount of precipitate: get the 100ml sheath fluid and divide several times to fall in double dish, 50 ℃ of lower oven dry, observe salt after the oven dry and analyze situation, the g of unit.
The measurement result of conductivity and the amount of separating out sees Table 3.
The conductivity of table 3 sheath fluid and the amount of precipitate
| ? | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | Embodiment 7 | Embodiment 8 |
| Conductivity | 6.05 | 6.23 | 6.46 | 11.98 | 23.03 | 5.85 | 29.93 | 30.17 |
| The amount of separating out | 0.3208 | 0.3285 | 0.3327 | 0.6423 | 1.6239 | 0.6774 | 1.8302 | 1.8316 |
Result by above-mentioned specific embodiment and mensuration can find out:
1, the surfactant sorbitol polyoxyethylene ether is in the concentration range of 100-2000mg/L, conductivity is less less than 40mS/m, the salt amount of analyzing, therefore, even there is sheath fluid residual in the instrument, also can not affect the detection of next sample, and lighter to the corrosion of instrument metal part;
When 2, the working concentration of sodium chloride was less than 16g/L in the sheath fluid, the salt amount of analyzing was all less, was difficult for corrosion instrument metal part;
3, the sodium chloride in the sheath fluid can be replaced by sodium sulphate, but the preferred sodium chloride that uses;
4, sheath fluid can use sodium chloride and sodium sulphate simultaneously, and conductivity is also less less than 40mS/m, the salt amount of analyzing;
5, replace glycerine with three polyglycereol, the conductivity of sheath fluid is also less less than 40mS/m, the salt amount of analyzing, and therefore infers, all can replace glycerine to use with a polyglycereol, two polyglycereol or five polyglycereol.
The sheath fluid Operability Testing
The sheath fluid of embodiment 8 is used respectively Japanese Sysmex UF series full automatic urine analyzer, the auspicious serial urine sediments analyzer of Changchun enlightening, Changsha to be liked to use in the instruments such as little urine sediments analyzer, Hangzhou Longxin urine sediments analyzer, find through using, observing, sheath fluid of the present invention both had been applicable to import instrument, also taken into account the result of use of domestic equipment, compare use cost with the import matched reagent and obtained effective control, compare with domestic reagent and have good result of use; In addition, sheath fluid of the present invention, lighter to the corrosion of instrument, greatly reduce the use and maintenance cost of equipment.
Need to prove that above-described embodiment does not limit the present invention in any form, all employings are equal to replaces or technical scheme that the mode of equivalent transformation obtains, all drops in protection scope of the present invention.
Claims (10)
1. the urine sediment analysis sheath fluid is characterized in that, comprising: mother liquor is dissolved in sodium chloride and the glycerols material of above-mentioned mother liquor; Be dissolved with in the above-mentioned mother liquor: buffering agent, surfactant, sequestrant, the pH value of mother liquor is 7-8.
2. urine sediment analysis sheath fluid according to claim 1 is characterized in that, also comprises in the above-mentioned mother liquor: mildewproof agent and antiseptic, preferred GXL and pyrithione.
3. urine sediment analysis sheath fluid according to claim 1 is characterized in that, above-mentioned glycerols material comprises: glycerine and polyglycereol, and preferably glycerine, the preferred working concentration of glycerine is 50-60g/L.
4. urine sediment analysis sheath fluid according to claim 1 is characterized in that, above-mentioned surfactant is Polyoxyethylene elher surfactant.
5. urine sediment analysis sheath fluid according to claim 4 is characterized in that, above-mentioned Polyoxyethylene elher surfactant is sorbitol polyoxyethylene ether, and working concentration is 100-2000mg/L, preferred 300 mg/L.
6. urine sediment analysis sheath fluid according to claim 1 is characterized in that, the working concentration of above-mentioned sodium chloride is less than 16g/L.
7. urine sediment analysis sheath fluid according to claim 1 is characterized in that, also comprises: sodium sulphate, working concentration is less than 14g/L.
8. prepare the described urine sediment analysis of claim 1 to 7 any one with the method for sheath fluid, it is characterized in that, may further comprise the steps:
(1), buffering agent, surfactant, sequestrant are dissolved in the deionized water, make the pH value and be the mother liquor of 7-8;
(2), sodium chloride is dissolved in the above-mentioned mother liquor, obtain intermediate solution;
(3), the glycerols material is dissolved in the above-mentioned intermediate solution, obtain sheath fluid.
9. the urine sediment analysis for preparing according to claim 8 is characterized in that with the method for sheath fluid, in step (1), continues to add mildewproof agent and antiseptic in above-mentioned mother liquor.
10. the urine sediment analysis for preparing according to claim 8 is characterized in that with the method for sheath fluid, in step (2), continues to add sodium sulphate in intermediate solution.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9470618B2 (en) | 2013-03-15 | 2016-10-18 | Iris International, Inc. | Sheath fluid systems and methods for particle analysis in blood samples |
| CN111781128A (en) * | 2020-06-15 | 2020-10-16 | 迪瑞医疗科技股份有限公司 | Sheath fluid for urine analyzer and preparation method thereof |
| US11525766B2 (en) | 2013-03-15 | 2022-12-13 | Iris International, Inc. | Dynamic range extension systems and methods for particle analysis in blood samples |
| CN116448990A (en) * | 2023-05-09 | 2023-07-18 | 中夏医学科技(青岛)有限公司 | Sheath liquid for analyzing urinary sediment |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9470618B2 (en) | 2013-03-15 | 2016-10-18 | Iris International, Inc. | Sheath fluid systems and methods for particle analysis in blood samples |
| US10451612B2 (en) | 2013-03-15 | 2019-10-22 | Iris International, Inc. | Sheath fluid systems and methods for particle analysis in blood samples |
| US11525766B2 (en) | 2013-03-15 | 2022-12-13 | Iris International, Inc. | Dynamic range extension systems and methods for particle analysis in blood samples |
| US11543340B2 (en) | 2013-03-15 | 2023-01-03 | Iris International, Inc. | Autofocus systems and methods for particle analysis in blood samples |
| CN111781128A (en) * | 2020-06-15 | 2020-10-16 | 迪瑞医疗科技股份有限公司 | Sheath fluid for urine analyzer and preparation method thereof |
| CN116448990A (en) * | 2023-05-09 | 2023-07-18 | 中夏医学科技(青岛)有限公司 | Sheath liquid for analyzing urinary sediment |
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Application publication date: 20130327 |