CN102988665A - Method for preparing gynecological menstruation regulating tablets and application - Google Patents
Method for preparing gynecological menstruation regulating tablets and application Download PDFInfo
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- 230000001105 regulatory effect Effects 0.000 title abstract description 5
- 238000002360 preparation method Methods 0.000 claims abstract description 23
- 239000003814 drug Substances 0.000 claims abstract description 21
- 235000021419 vinegar Nutrition 0.000 claims abstract description 19
- 239000000052 vinegar Substances 0.000 claims abstract description 19
- 238000000874 microwave-assisted extraction Methods 0.000 claims abstract description 11
- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 10
- 241000218176 Corydalis Species 0.000 claims abstract description 7
- 208000002454 Nasopharyngeal Carcinoma Diseases 0.000 claims abstract description 5
- 206010061306 Nasopharyngeal cancer Diseases 0.000 claims abstract description 5
- 201000011216 nasopharynx carcinoma Diseases 0.000 claims abstract description 5
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- 229920002472 Starch Polymers 0.000 claims description 5
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- 229940126678 chinese medicines Drugs 0.000 claims description 5
- 230000006837 decompression Effects 0.000 claims description 5
- 239000003480 eluent Substances 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 5
- 239000008187 granular material Substances 0.000 claims description 5
- 238000004064 recycling Methods 0.000 claims description 5
- 229920005989 resin Polymers 0.000 claims description 5
- 239000011347 resin Substances 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
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- 230000005764 inhibitory process Effects 0.000 claims description 3
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 abstract description 9
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 abstract description 9
- 229940114124 ferulic acid Drugs 0.000 abstract description 9
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 abstract description 9
- 235000001785 ferulic acid Nutrition 0.000 abstract description 9
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 abstract description 9
- 230000002401 inhibitory effect Effects 0.000 abstract description 3
- 241000213006 Angelica dahurica Species 0.000 abstract 1
- 241000132012 Atractylodes Species 0.000 abstract 1
- 240000004670 Glycyrrhiza echinata Species 0.000 abstract 1
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 abstract 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 abstract 1
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 abstract 1
- 241000212322 Levisticum officinale Species 0.000 abstract 1
- 241000736199 Paeonia Species 0.000 abstract 1
- 244000236658 Paeonia lactiflora Species 0.000 abstract 1
- 235000008598 Paeonia lactiflora Nutrition 0.000 abstract 1
- 235000006484 Paeonia officinalis Nutrition 0.000 abstract 1
- 241000405414 Rehmannia Species 0.000 abstract 1
- 244000126002 Ziziphus vulgaris Species 0.000 abstract 1
- 239000001645 levisticum officinale Substances 0.000 abstract 1
- 229940010454 licorice Drugs 0.000 abstract 1
- 229960004756 ethanol Drugs 0.000 description 21
- 238000002474 experimental method Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000013558 reference substance Substances 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
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- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 3
- 229960000935 dehydrated alcohol Drugs 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
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- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- 208000004998 Abdominal Pain Diseases 0.000 description 1
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- 208000019255 Menstrual disease Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 229920002334 Spandex Polymers 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
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- FCPVYOBCFFNJFS-LQDWTQKMSA-M benzylpenicillin sodium Chemical compound [Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 FCPVYOBCFFNJFS-LQDWTQKMSA-M 0.000 description 1
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- JWHHANVGNNWIRI-UHFFFAOYSA-N methanol phosphoric acid hydrate Chemical compound O.OC.OP(O)(O)=O JWHHANVGNNWIRI-UHFFFAOYSA-N 0.000 description 1
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- 229910052757 nitrogen Inorganic materials 0.000 description 1
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 description 1
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- 238000010561 standard procedure Methods 0.000 description 1
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Abstract
The invention provides a method for preparing gynecological menstruation regulating tablets. The menstruation regulating tablets are prepared from 144g of Chinese angelica, 16g of Szechuan lovage rhizome, 400g of vinegar baked rhizoma cyperi, 23g of bran baked bighead atractylodes rhizome, 12g of white peony root, 12g of red peony root, 32g of vinegar baked corydalis tuber, 48g of prepared rehmannia root, 80g of jujube and 11g of licorice serving as raw medicaments by adopting supercritical extraction and microwave extraction, so that the ferulic acid content is greatly improved. The invention also provides application of the gynecological menstruation regulating tablets in preparation of a medicament for inhibiting propagation of human nasopharyngeal carcinoma CNE-2Z cells.
Description
Technical field
The present invention relates to the Chinese medicine preparation technical field, be specifically related to a kind of preparation method and application of Fuke Tiaojing tablets.
Background technology
Fuke Tiaojing tablets is recorded in Ministry of Public Health standard WS3-B-0937-91, made as crude drug by Radix Angelicae Sinensis 144g, Rhizoma Chuanxiong 16g, Rhizoma Cyperi (processed with vinegar) 400g, Rhizoma Atractylodis Macrocephalae (parched with bran) 23g, Radix Paeoniae Alba 12g, Radix Paeoniae Rubra 12g, Rhizoma Corydalis (processed with vinegar) 32g, Radix Rehmanniae Preparata 48g, Fructus Jujubae 80g, Radix Glycyrrhizae 11g, can nourish blood, regulating menstruation, pain relieving.Be used for menoxenia, abdominal pain in menstruation.
In the prior art, not yet there is Fuke Tiaojing tablets to adopt the report of supercritical and microwave technology aspect the preparation extracting, and adopts the method that powder and decocting boil of beating, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and used clinically.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of preparation method of Fuke Tiaojing tablets.
Another object of the present invention is to provide the application of a kind of Fuke Tiaojing tablets in preparation inhibition Human Nasopharyngeal Carcinoma CNE-2 Z Cells propagation medicine.
Technical scheme: the objective of the invention is to realize by following scheme:
A kind of preparation method of Fuke Tiaojing tablets, by Radix Angelicae Sinensis 144g, Rhizoma Chuanxiong 16g, Rhizoma Cyperi (processed with vinegar) 400g, Rhizoma Atractylodis Macrocephalae (parched with bran) 23g, Radix Paeoniae Alba 12g, Radix Paeoniae Rubra 12g, Rhizoma Corydalis (processed with vinegar) 32g, Radix Rehmanniae Preparata 48g, Fructus Jujubae 80g, Radix Glycyrrhizae 11g makes as crude drug, described method is comprised of the following step: get Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi (processed with vinegar), join in the CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO2 flow 1-3m1/g crude drug min, extraction time 150-180min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W extracts 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
The preparation method of above-mentioned a kind of Fuke Tiaojing tablets, described CO
2The percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
The preparation method of above-mentioned a kind of Fuke Tiaojing tablets, described microwave extracting power 500W extracts 6 minutes at every turn.
The preparation method of above-mentioned a kind of Fuke Tiaojing tablets, described CO
2The extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2Flow 2ml/g crude drug min, extraction time 160min.
The application of above-mentioned Fuke Tiaojing tablets in preparation inhibition Human Nasopharyngeal Carcinoma CNE-2 Z Cells propagation medicine.
In the prior art, every 0.5g of Fuke Tiaojing tablets, each 4,3 times on the one, the every 0.5g of Fuke Tiaojing tablets that adopts the present invention to be prepared into only needs 2 at every turn, takes 3 times in 1st, has greatly reduced dose having under the condition of more active component.This conclusion can be by following evidence.
The comparison of ferulaic acid content in the Fuke Tiaojing tablets of test one, distinct methods preparation
1, instrument and reagent Fuke Tiaojing tablets of the present invention: press the preparation of embodiment 3 methods, use the 1669g crude drug, make 1000 through extraction, every heavy 0.5g.Former Fuke Tiaojing tablets by the preparation of ministry standard method, uses the 1669g crude drug, makes 1000 through extraction, every heavy 0.5g.Agilent 1200 high performance liquid chromatographs; METTLER AE240 electronic analytical balance; Ferulic acid reference substance (Nat'l Pharmaceutical ﹠ Biological Products Control Institute).
2, method
Chromatographic condition and system suitability: be filler with octadecylsilane chemically bonded silica; Methanol-water-phosphoric acid (40:60:0.2) is mobile phase; The detection wavelength is 280nm.Number of theoretical plate is pressed the ferulic acid peak and is calculated, and should be not less than 3000.
The preparation of reference substance solution: precision takes by weighing at 4 hours ferulic acid reference substance of 60 ℃ of drying under reduced pressure an amount of, adds methanol and makes the solution that every 1ml contains 18 μ g, and get final product.
The preparation of need testing solution: get Fuke Tiaojing tablets of the present invention and former Fuke Tiaojing tablets, porphyrize, mixing is got 1g, and is accurately weighed, the accurate 70% ethanol 20ml that adds, close plug, supersound process 10 minutes, centrifugal, get supernatant, and get final product.
Algoscopy is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
3, result
The result shows that the content of ferulic acid is the 1.56mg/ sheet in the Fuke Tiaojing tablets of the present invention; And the content of ferulic acid is the 0.28mg/ sheet in the former Fuke Tiaojing tablets, and in the situation that dose reduces, ferulaic acid content improves a lot.
Above-mentioned studies show that, the Fuke Tiaojing tablets that adopts the present invention to prepare, active constituent content is higher than the standby Fuke Tiaojing tablets of ministry standard legal system.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following example, all technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Radix Angelicae Sinensis 144g, Rhizoma Chuanxiong 16g, Rhizoma Cyperi (processed with vinegar) 400g, Rhizoma Atractylodis Macrocephalae (parched with bran) 23g, Radix Paeoniae Alba 12g, Radix Paeoniae Rubra 12g, Rhizoma Corydalis (processed with vinegar) 32g, Radix Rehmanniae Preparata 48g, Fructus Jujubae 80g, Radix Glycyrrhizae 11g, with Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi (processed with vinegar), join in the CO2 supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4%, extracting pressure 15MPa, 30 ℃ of temperature, CO2 flow 1m1/g crude drug min, extraction time 150min, get supercritical extract, for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 400W extracts 2 times, each 4 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
After testing, the content of ferulic acid is the 1.59mg/ sheet in the finished product.
Embodiment 2
Get Radix Angelicae Sinensis 144g, Rhizoma Chuanxiong 16g, Rhizoma Cyperi (processed with vinegar) 400g, Rhizoma Atractylodis Macrocephalae (parched with bran) 23g, Radix Paeoniae Alba 12g, Radix Paeoniae Rubra 12g, Rhizoma Corydalis (processed with vinegar) 32g, Radix Rehmanniae Preparata 48g, Fructus Jujubae 80g, Radix Glycyrrhizae 11g, with Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi (processed with vinegar), join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 6%, extracting pressure 30MPa, temperature 50 C, CO
2Flow 3m1/g crude drug min, extraction time 180min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 600W extracts 2 times, each 8 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
After testing, the content of ferulic acid is the 1.67mg/ sheet in the finished product.
Embodiment 3
Get Radix Angelicae Sinensis 144g, Rhizoma Chuanxiong 16g, Rhizoma Cyperi (processed with vinegar) 400g, Rhizoma Atractylodis Macrocephalae (parched with bran) 23g, Radix Paeoniae Alba 12g, Radix Paeoniae Rubra 12g, Rhizoma Corydalis (processed with vinegar) 32g, Radix Rehmanniae Preparata 48g, Fructus Jujubae 80g, Radix Glycyrrhizae 11g, with Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi (processed with vinegar), join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 20MPa, 40 ℃ of temperature, CO
2Flow 2m1/g crude drug min, extraction time 160min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 500W extracts 2 times, each 6 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
After testing, the content of ferulic acid is the 1.56mg/ sheet in the finished product.
Embodiment 4: Fuke Tiaojing tablets suppresses the experimentation data of CNE-2Z cell proliferation
1 experiment material
1.1 experiment cell strain
Human Nasopharyngeal Carcinoma CNE-2 Z Cells, Nanjing Zhengkuan Pharmaceutical Technology Co., Ltd.'s laboratory cell bank, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: Fuke Tiaojing tablets of the present invention: press the preparation of embodiment 3 methods.
The medicinal liquid liquid storage: take by weighing the 100mg Fuke Tiaojing tablets, be dissolved in the 5ml dehydrated alcohol, 0.2 μ m filter filters, and 500 μ ldoff manage packing ,-20 ℃ of storages, and 0.2 μ m filter filters dehydrated alcohol in order to the usefulness of matched group simultaneously.
1.3 experiment reagent
The Cat.No.12100-061 Lot.No.758137 of DMEM(GIBCO company); Hyclone (Hangzhoupro, sky, Zhejiang bio tech ltd Lot.No.100419); NaHCO3(Shanghai hundred million chemical reagent company limited Cat.No.11810-033Lot.No.1088387 of a specified duration); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt(AMRESCO company lot number: 2010242); Streptomycin Sulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); The autogamy of PBS(laboratory);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); As seen-ultraviolet light microwell plate detector (U.S. MD company model: SPECTRAMAX 190); CO2 incubator (FORMA model: 3111); (safe and sound company of Su Jing group makes model to super-clean bench: SW-CJ-ZFD); Pure water instrument (U.S. Spring company model: S/N 020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (the accurate experimental facilities in Shanghai company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) the CNE-2Z cell carries out cellar culture (10cm culture dish) with DMEM+10%FBS in 37 ℃, 5%CO2, when Growth of Cells during to logarithmic (log) phase, collecting cell discards culture fluid, PBS fine laundering 3 times, add 3ml 0.25% trypsin-0.04%EDTA, behind 37 ℃ of digestion 2min, to wherein adding 5ml complete medium neutralization reaction, behind the piping and druming cell it is changed in the centrifuge tube, the centrifugal 5min of 1000rpm adjusts 3 * 104/ml of concentration of cell suspension.
2) the cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, culture plate put into cell culture incubator (37 ℃, 5%CO2) cellar culture.
3) according to the Growth of Cells situation, generally grow to 50%-70%, add Fuke Tiaojing tablets solution, continue to cultivate 24h.
4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) behind the 24h, continue to cultivate 4h.
5) the buckle method is removed supernatant behind the 4h, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on the shaking table, and crystal is fully dissolved.Measure the light absorption value in each hole at enzyme-linked immunosorbent assay instrument 490nm place.
6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide) is set 6 multiple holes for every group.
7) result represents with the suppression ratio of medicine to cell:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value * 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel 2003 softwares, data represent with mean ± S.D..
4 experimental results
Statistical result showed after the mtt assay experiment, compare with matched group, when dosage reaches 5mg/ml, to CNE-2Z cell inhibitory effect variant (P＜0.05), dosage this difference when 10mg/ml has significance (P＜0.01), and utmost point significant difference (P＜0.001) is arranged when dosage reaches 15-20mg/ml.
Table 1 Fuke Tiaojing tablets affects the CNE-2Z cell inhibitory effect
(X ± SD)
Annotate: compare * P＜0.01 with matched group; * P＜0.001
5 experiment conclusion
Fuke Tiaojing tablets can suppress the CNE-2Z cell proliferation, reduces the Growth of Cells number of CNE-2Z cell, and this effect is dose dependent.
Claims (5)
1. the preparation method of a Fuke Tiaojing tablets, made as crude drug by Radix Angelicae Sinensis 144g, Rhizoma Chuanxiong 16g, Rhizoma Cyperi (processed with vinegar) 400g, Rhizoma Atractylodis Macrocephalae (parched with bran) 23g, Radix Paeoniae Alba 12g, Radix Paeoniae Rubra 12g, Rhizoma Corydalis (processed with vinegar) 32g, Radix Rehmanniae Preparata 48g, Fructus Jujubae 80g, Radix Glycyrrhizae 11g, it is characterized in that described method is comprised of the following step: get Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi (processed with vinegar), join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2Flow 1-3m1/g crude drug min, extraction time 150-180min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W extracts 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
2. the preparation method of described a kind of Fuke Tiaojing tablets according to claim 1 is characterized in that described CO
2The percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
3. the preparation method of described a kind of Fuke Tiaojing tablets according to claim 1 is characterized in that described microwave extracting power 500W, extracts 6 minutes at every turn.
4. the preparation method of described a kind of Fuke Tiaojing tablets according to claim 1 is characterized in that described CO
2The extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2Flow 2ml/g crude drug min, extraction time 160min.
5. the according to claim 1 application of described a kind of Fuke Tiaojing tablets in preparation inhibition Human Nasopharyngeal Carcinoma CNE-2 Z Cells propagation medicine.
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