CN102988526A - Method for preparing Bailing tablets and application - Google Patents
Method for preparing Bailing tablets and application Download PDFInfo
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- CN102988526A CN102988526A CN201210377391XA CN201210377391A CN102988526A CN 102988526 A CN102988526 A CN 102988526A CN 201210377391X A CN201210377391X A CN 201210377391XA CN 201210377391 A CN201210377391 A CN 201210377391A CN 102988526 A CN102988526 A CN 102988526A
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- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 10
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- 230000005764 inhibitory process Effects 0.000 claims description 3
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 abstract description 9
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 abstract description 9
- 229940114124 ferulic acid Drugs 0.000 abstract description 9
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 abstract description 9
- 235000001785 ferulic acid Nutrition 0.000 abstract description 9
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 abstract description 9
- 230000002401 inhibitory effect Effects 0.000 abstract description 3
- 244000170916 Paeonia officinalis Species 0.000 abstract 2
- 235000006484 Paeonia officinalis Nutrition 0.000 abstract 2
- 244000144730 Amygdalus persica Species 0.000 abstract 1
- 244000061520 Angelica archangelica Species 0.000 abstract 1
- 241000213006 Angelica dahurica Species 0.000 abstract 1
- 241000132012 Atractylodes Species 0.000 abstract 1
- 244000020518 Carthamus tinctorius Species 0.000 abstract 1
- 235000003255 Carthamus tinctorius Nutrition 0.000 abstract 1
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- 208000031671 Large B-Cell Diffuse Lymphoma Diseases 0.000 abstract 1
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- 244000234609 Portulaca oleracea Species 0.000 abstract 1
- 235000001855 Portulaca oleracea Nutrition 0.000 abstract 1
- 235000006040 Prunus persica var persica Nutrition 0.000 abstract 1
- 241001180876 Saposhnikovia Species 0.000 abstract 1
- 229940107666 astragalus root Drugs 0.000 abstract 1
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- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000013558 reference substance Substances 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
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- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
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- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 229920002334 Spandex Polymers 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
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- FCPVYOBCFFNJFS-LQDWTQKMSA-M benzylpenicillin sodium Chemical compound [Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 FCPVYOBCFFNJFS-LQDWTQKMSA-M 0.000 description 1
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- JWHHANVGNNWIRI-UHFFFAOYSA-N methanol phosphoric acid hydrate Chemical compound O.OC.OP(O)(O)=O JWHHANVGNNWIRI-UHFFFAOYSA-N 0.000 description 1
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- 229910052757 nitrogen Inorganic materials 0.000 description 1
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a method for preparing Bailing tablets. The Bailing tablets are prepared from 194g of Chinese angelica, 42g of pseudo-ginseng, 83g of safflower, 167g of peony bark, 83g of peach kernel, 167g of divaricate saposhnikovia root, 167g of atractylodes, 42g of angelica root, 417g of purslane, 83g of red peony root and 194g of astragalus root serving as raw medicaments by adopting supercritical extraction and microwave extraction, so that the ferulic acid content is greatly improved. The invention also provides application of the Bailing tablets in preparation of a medicament for inhibiting propagation of human histiocytic lymphoma U937 cells.
Description
Technical field
The present invention relates to the Chinese medicine preparation technical field, be specifically related to a kind of preparation method and application of BAILING PIAN.
Background technology
BAILING PIAN is recorded in Ministry of Public Health standard WS3-B-0914-91, made as crude drug by Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, Radix Astragali 194g, the energy blood circulation promoting and blood stasis dispelling increases photosensitization.Be used for vitiligo.
In the prior art, not yet there is BAILING PIAN to adopt the report of supercritical and microwave technology aspect the preparation extracting, and adopts the method that powder and decocting boil of beating, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and used clinically.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of preparation method of BAILING PIAN.
Another object of the present invention is to provide the application of a kind of BAILING PIAN in the preparation inhibition lymphoma U937 of human tissue cell cell proliferation medicine.
Technical scheme: the objective of the invention is to realize by following scheme:
A kind of preparation method of BAILING PIAN, made as crude drug by Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, Radix Astragali 194g, described method is comprised of the following step: get Radix Angelicae Sinensis, Flos Carthami, Semen Persicae, Rhizoma Atractylodis, join in the CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2Flow 1-3ml/g crude drug min, extraction time 150-180min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W extracts 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
The preparation method of above-mentioned a kind of BAILING PIAN, described CO
2The percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
The preparation method of above-mentioned a kind of BAILING PIAN, described microwave extracting power 500W extracts 6 minutes at every turn.
The preparation method of above-mentioned a kind of BAILING PIAN, described CO
2The extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2Flow 2ml/g crude drug min, extraction time 160min.
The application of above-mentioned BAILING PIAN in the preparation inhibition lymphoma U937 of human tissue cell cell proliferation medicine.
In the prior art, every 0.5g of BAILING PIAN, each 4,3 times on the one, the every 0.5g of BAILING PIAN that adopts the present invention to be prepared into only needs 2 at every turn, takes 3 times in 1st, has greatly reduced dose having under the condition of more active component.This conclusion can be by following evidence.
The comparison of ferulaic acid content in the BAILING PIAN of test one, distinct methods preparation
1, instrument and reagent BAILING PIAN of the present invention: press the preparation of embodiment 3 methods, use the 1639g crude drug, make 1000 through extraction, every heavy 0.5g.Former BAILING PIAN by the preparation of ministry standard method, is used the 1639g crude drug, makes 1000 through extraction, every heavy 0.5g.Agilent 1200 high performance liquid chromatographs; METTLER AE240 electronic analytical balance; Ferulic acid reference substance (Nat'l Pharmaceutical ﹠ Biological Products Control Institute).
2, method
Chromatographic condition and system suitability: be filler with octadecylsilane chemically bonded silica; Methanol-water-phosphoric acid (40:60:0.2) is mobile phase; The detection wavelength is 280nm.Number of theoretical plate is pressed the ferulic acid peak and is calculated, and should be not less than 3000.
The preparation of reference substance solution: precision takes by weighing at 4 hours ferulic acid reference substance of 60 ℃ of drying under reduced pressure an amount of, adds methanol and makes the solution that every 1ml contains 18 μ g, and get final product.
The preparation of need testing solution: get BAILING PIAN of the present invention and former BAILING PIAN, porphyrize, mixing is got 1g, and is accurately weighed, the accurate 70% ethanol 20ml that adds, close plug, supersound process 10 minutes, centrifugal, get supernatant, and get final product.
Algoscopy is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
3, result
The result shows that the content of ferulic acid is the 1.53mg/ sheet in the BAILING PIAN of the present invention; And the content of ferulic acid is the 0.28mg/ sheet in the former BAILING PIAN, and in the situation that dose reduces, ferulaic acid content improves a lot.
Above-mentioned studies show that, the BAILING PIAN that adopts the present invention to prepare, active constituent content is higher than the standby BAILING PIAN of ministry standard legal system.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following example, all technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, the Radix Astragali 194, with Radix Angelicae Sinensis, Flos Carthami, Semen Persicae, Rhizoma Atractylodis, join in the CO2 supercritical extraction device, ethanol is as entrainer, the percent by volume that entrainer accounts for total extractant is 4%, extracting pressure 15MPa, 30 ℃ of temperature, CO
2Flow 1ml/g crude drug min, extraction time 150min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 400W extracts 2 times, each 4 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
After testing, the content of ferulic acid is the 1.64mg/ sheet in the finished product.
Embodiment 2
Get Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, the Radix Astragali 194, with Radix Angelicae Sinensis, Flos Carthami, Semen Persicae, Rhizoma Atractylodis, join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 6%, extracting pressure 30MPa, temperature 50 C, CO
2Flow 3ml/g crude drug min, extraction time 180min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 600W extracts 2 times, each 8 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
After testing, the content of ferulic acid is the 1.61mg/ sheet in the finished product.
Embodiment 3
Get Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, the Radix Astragali 194, with Radix Angelicae Sinensis, Flos Carthami, Semen Persicae, Rhizoma Atractylodis, join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 20MPa, 40 ℃ of temperature, CO
2Flow 2m1/g crude drug min, extraction time 160min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 500W extracts 2 times, each 6 minutes, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
After testing, the content of ferulic acid is the 1.53mg/ sheet in the finished product.
Embodiment 4: BAILING PIAN suppresses the experimentation data of U937 cell proliferation
1 experiment material
1.1 experiment cell strain
The lymphoma U937 of human tissue cell cell, Nanjing Zhengkuan Pharmaceutical Technology Co., Ltd.'s laboratory cell bank, DMEM+10% FBS cellar culture.
1.2 Experimental agents
Drugs: BAILING PIAN of the present invention: press the preparation of embodiment 3 methods.
The medicinal liquid liquid storage: take by weighing the 100mg BAILING PIAN, be dissolved in the 5ml dehydrated alcohol, 0.2 μ m filter filters, and 500 μ l doff manage packing ,-20 ℃ of storages, and 0.2 μ m filter filters dehydrated alcohol in order to the usefulness of matched group simultaneously.
1.3 experiment reagent
The Cat.No.12100-061 Lot.No.758137 of DMEM(GIBCO company); Hyclone (Hangzhoupro, sky, Zhejiang bio tech ltd Lot.No.100419); NaHCO3(Shanghai hundred million chemical reagent company limited Cat.No.11810-033 Lot.No.1088387 of a specified duration); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt(AMRESCO company lot number: 2010242); Streptomycin Sulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); The autogamy of PBS(laboratory);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); As seen-ultraviolet light microwell plate detector (U.S. MD company model: SPECTRA MAX 190); CO2 incubator (FORMA model: 3111); (safe and sound company of Su Jing group makes model to super-clean bench: SW-CJ-ZFD); Pure water instrument (U.S. Spring company model: S/N 020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (the accurate experimental facilities in Shanghai company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) the U937 cell carries out cellar culture (10cm culture dish) with DMEM+10% FBS in 37 ℃, 5% CO2, when Growth of Cells during to logarithmic (log) phase, collecting cell discards culture fluid, PBS fine laundering 3 times, add 3ml 0.25% trypsin-0.04% EDTA, behind 37 ℃ of digestion 2min, to wherein adding 5ml complete medium neutralization reaction, behind the piping and druming cell it is changed in the centrifuge tube, the centrifugal 5min of 1000rpm adjusts 3 * 104/ml of concentration of cell suspension.
2) the cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, and culture plate is put into cell culture incubator (37 ℃, 5% CO2) cellar culture.
3) according to the Growth of Cells situation, generally grow to 50%-70%, add BAILING PIAN solution, continue to cultivate 24h.
4) add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT) behind the 24h, continue to cultivate 4h.
5) the buckle method is removed supernatant behind the 4h, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on the shaking table, and crystal is fully dissolved.Measure the light absorption value in each hole at enzyme-linked immunosorbent assay instrument 490nm place.6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide) is set 6 multiple holes for every group.
7) result represents with the suppression ratio of medicine to cell:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value * 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel 2003 softwares, data represent with mean ± S.D..
4 experimental results
Statistical result showed after the mtt assay experiment, compare with matched group, when dosage reaches 5mg/ml, to U937 cell inhibitory effect variant (P＜0.05), dosage this difference when 10mg/ml has significance (P＜0.01), and utmost point significant difference (P＜0.001) is arranged when dosage reaches 15-20mg/ml.
Table 1 BAILING PIAN is on U937 cell inhibitory effect impact research
Annotate: compare * P＜0.01 with matched group; * P＜0.001
5 experiment conclusion
BAILING PIAN can suppress the U937 cell proliferation, reduces the Growth of Cells number of U937 cell, and this effect is dose dependent.
Claims (5)
1. the preparation method of a BAILING PIAN, made as crude drug by Radix Angelicae Sinensis 194g, Radix Notoginseng 42g, Flos Carthami 83g, Cortex Moutan 167g, Semen Persicae 83g, Radix Saposhnikoviae 167g, Rhizoma Atractylodis 167g, Radix Angelicae Dahuricae 42g, Herba Portulacae 417g, Radix Paeoniae Rubra 83g, Radix Astragali 194g, it is characterized in that described method is comprised of the following step: get Radix Angelicae Sinensis, Flos Carthami, Semen Persicae, Rhizoma Atractylodis, join CO
2In the supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 ℃, CO
2Flow 1-3m1/g crude drug min, extraction time 150-180min gets supercritical extract, and is for subsequent use; Get all the other Chinese medicines, pulverize, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W extracts 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on the D101 macroporous adsorptive resins, 50% ethanol elution is collected 5 times of amount column volume eluents, decompression recycling ethanol, concentrated and dry, get the microwave extraction thing, for subsequent use; Above-mentioned supercritical extract and microwave extraction thing are mixed, add starch, 70% ethanol granule processed, drying, tabletting is made 1000, every heavy 0.5g.
2. the preparation method of described a kind of BAILING PIAN according to claim 1 is characterized in that described CO
2The percent by volume that the supercritical extraction entrainer accounts for total extractant is 5%.
3. the preparation method of described a kind of BAILING PIAN according to claim 1 is characterized in that described microwave extracting power 500W, extracts 6 minutes at every turn.
4. the preparation method of described a kind of BAILING PIAN according to claim 1 is characterized in that described CO
2The extracting pressure 20MPa of supercritical extraction, 40 ℃ of temperature, CO
2Flow 2ml/g crude drug min, extraction time 160min.
5. the according to claim 1 application of described a kind of BAILING PIAN in the preparation inhibition lymphoma U937 of human tissue cell cell proliferation medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210377391 CN102988526B (en) | 2012-10-08 | 2012-10-08 | Method for preparing Bailing tablets and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210377391 CN102988526B (en) | 2012-10-08 | 2012-10-08 | Method for preparing Bailing tablets and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103655840A (en) * | 2013-12-11 | 2014-03-26 | 山东中大药业有限公司 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse leukaemia cell L1210 proliferation |
| CN103655842A (en) * | 2013-12-11 | 2014-03-26 | 山东中大药业有限公司 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lymphomata cell EL4. IL-2 proliferation |
| CN103655841A (en) * | 2013-12-11 | 2014-03-26 | 山东中大药业有限公司 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lymphoid cancer cell P388D1 proliferation |
| CN103655843A (en) * | 2013-12-11 | 2014-03-26 | 山东中大药业有限公司 | Yikangbuyuan tablet, and preparation method and application thereof |
| CN103655838A (en) * | 2013-12-11 | 2014-03-26 | 山东中大药业有限公司 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lymphomata cell L5178Y proliferation |
| CN103655839A (en) * | 2013-12-11 | 2014-03-26 | 山东中大药业有限公司 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lung cancer cell LLC proliferation |
| CN103690646A (en) * | 2013-12-11 | 2014-04-02 | 山东中大药业有限公司 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicine for inhibiting mouse lymphoma cell YAC-1 cell proliferation |
| CN104306726A (en) * | 2014-11-13 | 2015-01-28 | 山东中大药业有限公司 | Preparation method of nine-herb false Chinese swertia herb tablets and application of nine-herb false Chinese swertia herb tablets in preparation of medicament for inhibiting cell proliferation of myeloma cell FO |
| CN104383282A (en) * | 2014-11-13 | 2015-03-04 | 山东中大药业有限公司 | Preparation method of nine-ingredient false Chinese swertia herb tablets and application of nine-ingredient false Chinese swertia herb tablets to preparation of medicines for inhibiting proliferation of hepatoma cell Hepa1-6 |
| CN104398815A (en) * | 2014-11-13 | 2015-03-11 | 山东中大药业有限公司 | Preparation method of nine flavor swertia bimaculata tablet and application of nine flavor swertia bimaculata tablet in preparation of drugs for inhibition of cell proliferation of melanoma cell B16 |
| CN104398818A (en) * | 2014-11-13 | 2015-03-11 | 山东中大药业有限公司 | Preparation method of nine flavor swertia bimaculata tablet and application of nine flavor swertia bimaculata tablet in preparation of drugs for inhibition of cell proliferation of myeloma cell SP2/0 |
| CN109329940A (en) * | 2018-12-14 | 2019-02-15 | 洛阳采方医药科技有限公司 | A kind of food grade antioxidants and preparation method thereof |
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| CN103655842B (en) * | 2013-12-11 | 2015-06-03 | 冯向英 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lymphomata cell EL4. IL-2 proliferation |
| CN103655838B (en) * | 2013-12-11 | 2015-06-24 | 邵文博 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lymphomata cell L5178Y proliferation |
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| CN103655840B (en) * | 2013-12-11 | 2015-07-15 | 周武元 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse leukaemia cell L1210 proliferation |
| CN103655842A (en) * | 2013-12-11 | 2014-03-26 | 山东中大药业有限公司 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lymphomata cell EL4. IL-2 proliferation |
| CN103655839B (en) * | 2013-12-11 | 2015-07-15 | 周武元 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lung cancer cell LLC proliferation |
| CN103655840A (en) * | 2013-12-11 | 2014-03-26 | 山东中大药业有限公司 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse leukaemia cell L1210 proliferation |
| CN103655841B (en) * | 2013-12-11 | 2015-05-20 | 刘伟伟 | Preparation method of Yikangbuyuan tablet and application of Yikangbuyuan tablet in medicines for inhibiting mouse lymphoid cancer cell P388D1 proliferation |
| CN104383282A (en) * | 2014-11-13 | 2015-03-04 | 山东中大药业有限公司 | Preparation method of nine-ingredient false Chinese swertia herb tablets and application of nine-ingredient false Chinese swertia herb tablets to preparation of medicines for inhibiting proliferation of hepatoma cell Hepa1-6 |
| CN104398818A (en) * | 2014-11-13 | 2015-03-11 | 山东中大药业有限公司 | Preparation method of nine flavor swertia bimaculata tablet and application of nine flavor swertia bimaculata tablet in preparation of drugs for inhibition of cell proliferation of myeloma cell SP2/0 |
| CN104398815A (en) * | 2014-11-13 | 2015-03-11 | 山东中大药业有限公司 | Preparation method of nine flavor swertia bimaculata tablet and application of nine flavor swertia bimaculata tablet in preparation of drugs for inhibition of cell proliferation of melanoma cell B16 |
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| CN109329940A (en) * | 2018-12-14 | 2019-02-15 | 洛阳采方医药科技有限公司 | A kind of food grade antioxidants and preparation method thereof |
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