CN102978116A - Microcystis culture medium formula - Google Patents
Microcystis culture medium formula Download PDFInfo
- Publication number
- CN102978116A CN102978116A CN2012104357536A CN201210435753A CN102978116A CN 102978116 A CN102978116 A CN 102978116A CN 2012104357536 A CN2012104357536 A CN 2012104357536A CN 201210435753 A CN201210435753 A CN 201210435753A CN 102978116 A CN102978116 A CN 102978116A
- Authority
- CN
- China
- Prior art keywords
- substratum
- microcystis
- culture medium
- microcystis aeruginosa
- aeruginosa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides a microcystis culture medium. The formula of the microcystis culture medium comprises 1-1.2g/L of NaNO3, 100-105mg/L of NH4NO3, 450-455mg/L of KNO3, 500-510mg/L of CaCl2.2H2O, 170-175mg/L of MgSO4.7H2O, 200-205mg/L of K2HPO4, 0.5-0.55mg/L of KI, 9.1-9.3mg/L of H3BO3, 20-20.5mg/L of MnSO4.4H2O, 5.3-5.5mg/L of ZnSO4.7H2O, 0.02-0.03mg/L of CuSO4.5H2O, 0.02-0.03mg/L of CoCl2.6H2O, 6-10mg/L of ferric citrate, 15-20mg/L of Na2-EDTA, 15-20mg/L of inositol, 0.2-0.3mg/L of nicotinic acid, 0.7-0.8mg/L of vitamin B12, 0.1-0.15mg/L of NNA and 0.05-0.08mg/L of IBA. The microcystis culture medium has the characteristics of high survival rate and fast growth rate when microcystis is cultured by the medium, and cannot be polluted by other bacteria.
Description
Technical field
The present invention relates to a kind of algae media, relate to especially the culture medium prescription of a kind of Microcystis aeruginosa.
Background technology
Microcystin (MCs) is a kind of ring seven peptide compound, its basic structure is ring (D-alanine-L-X-D-red-the different aspartic acid of methyl-β-D--L-Z-Adda-D-isoglutamic acid-N-methyl dehydroalanine), wherein N-methyl dehydroalanine is a kind of special amino acid, contain α, β unsaturated double-bond, the Adda structure is 3-amino-9-methoxyl group-2,6,8-trimethylammonium-10-phenyl-4, the 6-diolefinic acid.X, Y are respectively two kinds of variable L amino acid, and its amino acid whose replacement or other amino acid whose demethylation derive numerous Type of toxins, and wherein the MC-LR(molecular formula is C49H75N13O12) the toxicity maximum.
In recent years, the tap water seedbed blue-green alga blooms such as China Taihu Lake, Song Hua River, Huaihe River are seriously polluted, discharge a large amount of MC-LR to water body, the health problem that is caused by MC-LR comes into one's own day by day, and a large amount of scientific and technical research personnel are furtheing investigate the various toxicitys of MC-LR and resolution characteristic.Because MC-LR sample famine in the research process can only dependence on import.The MC-LR import standard substance price that specification is 10ug is up to 3500 yuan, so that the laboratory study cost is higher, general medium and small research institution is difficult to carry out correlative study work.
Therefore need in the laboratory, cultivate Microcystis aeruginosa so that its physiological property is studied, and be used for the extraction Microcystin.But the present rare report of cultural method of Microcystis aeruginosa, and be specifically designed to Microcystis aeruginosa substratum report just still less.
Summary of the invention
The invention provides a kind of substratum for Microcystis aeruginosa, the prescription of this substratum is as follows:
NaNO
31～1.2g/L，NH
4NO
3100～105mg/L，KNO
3450～455mg/L，CaCl
2.2H
2O500～510mg/L，MgSO
4.7H
2O 170～175mg/L，K
2HPO
4 200～205mg/L，KI0.5～0.55mg/L，H
3BO
3 9.1～9.3mg/L，MnSO
4.4H
2O 20～20.5mg/L，ZnSO
4.7H
2O 5.3～5.5mg/L，CuSO
4.5H
2O 0.02～0.03mg/L，CoCl
2.6H
2O 0.02～0.03mg/L；
Ironic citrate 6～10mg/L, Na
2-EDTA.2H
2O 15～20mg/L, inositol 15～20mg/L, nicotinic acid 0.2～0.3mg/L, vitamins B
120.7～0.8mg/L, NNA 0.1～0.15mg/L, IBA 0.05～0.08mg/L.
Preferably, in above-mentioned culture medium prescription, add again Mo (NO
3)
3.5H
2O 2～3mg/L.
Substratum of the present invention has the survival rate height when cultivating Microcystis aeruginosa, the characteristics that growth velocity is fast, and be difficult for by other bacterial contamination.The following examples and contrast experiment can clearly react the characteristics of substratum of the present invention.
Embodiment
Embodiment 1
Be formulated as follows the substratum of prescription:
NaNO
31g/L，NH
4NO
3100mg/L，KNO
3450mg/L，CaCl
2.2H
2O 500mg/L，MgSO
4.7H
2O 170mg/L，K
2HPO
4200mg/L，KI 0.5mg/L，H
3BO
39.1mg/L，MnSO
4.4H
2O 20mg/L ，ZnSO
4.7H
2O 5.3mg/L，Mo(NO
3)
3.5H
2O2mg/LCuSO
4.5H
2O 0.02mg/L，CoCl
2.6H
2O 0.02mg/L；
Ironic citrate 6mg/L, Na
2-EDTA.2H
2O 15mg/L, inositol 15mg/L, nicotinic acid 0.2mg/L, vitamins B
120.7mg/L, NNA 0.1mg/L, IBA 0.05mg/L.
The microcystic aeruginosa of taking the logarithm vegetative period is inoculated in this substratum and cultivates, illumination every day 8 hours, and the incubator temperature is controlled at about 25 degrees centigrade, the cell density of Microcystis aeruginosa in the per 3 days record substratum.
Embodiment 2
NaNO
31.2g/L，NH
4NO
3105mg/L，KNO
3455mg/L，CaCl
2.2H
2O 510mg/L，MgSO
4.7H
2O 175mg/L，K
2HPO
4205mg/L，KI 0.55mg/L，H
3BO
39.3mg/L，MnSO
4.4H
2O 20.5mg/L，ZnSO
4.7H
2O 5.5mg/L，Mo(NO
3)
3.5H
2O 3mg/L，CuSO
4.5H
2O 0.03mg/L，CoCl
2.6H
2O 0.03mg/L；
Ironic citrate 10mg/L, Na
2-EDTA.2H
2O 20mg/L, inositol 20mg/L, nicotinic acid 0.3mg/L, vitamins B
120.8mg/L, NNA 0.15mg/L, IBA 0.08mg/L.
The microcystic aeruginosa of taking the logarithm vegetative period is inoculated in this substratum and cultivates, illumination every day 8 hours, and the incubator temperature is controlled at about 25 degrees centigrade, the cell density of Microcystis aeruginosa in the per 3 days record substratum.
Embodiment 3
NaNO
31.1g/L，NH
4NO
3105mg/L，KNO
3455mg/L，CaCl
2.2H
2O 510mg/L，MgSO
4.7H
2O 175mg/L，K
2HPO
4205mg/L，KI 0.55mg/L，H
3BO
39.3mg/L，MnSO
4.4H
2O 20.5mg/L，ZnSO
4.7H
2O 5.5mg/L，Mo(NO
3)
3.5H
2O 2.5mg/L，CuSO
4.5H
2O 0.025mg/L，CoCl
2.6H
2O 0.025mg/L；
Ironic citrate 8mg/L, Na
2-EDTA.2H
2O 18mg/L, inositol 18mg/L, nicotinic acid 0.25mg/L, vitamins B
120.75mg/L, NNA 0.12mg/L, IBA 0.06mg/L.
The microcystic aeruginosa of taking the logarithm vegetative period is inoculated in this substratum and cultivates, illumination every day 8 hours, and the incubator temperature is controlled at about 25 degrees centigrade, the cell density of Microcystis aeruginosa in the per 3 days record substratum.
Embodiment 4
NaNO
31.1g/L，NH
4NO
3105mg/L，KNO
3455mg/L，CaCl
2.2H
2O 510mg/L，MgSO
4.7H
2O 175mg/L，K
2HPO
4205mg/L，KI 0.55mg/L，H
3BO
39.3mg/L，MnSO
4.4H
2O 20.5mg/L，ZnSO
4.7H
2O 5.5mg/L，CuSO
4.5H
2O 0.025mg/L，CoCl
2.6H
2O 0.025mg/L；
Ironic citrate 8mg/L, Na
2-EDTA.2H
2O 18mg/L, inositol 18mg/L, nicotinic acid 0.25mg/L, vitamins B
120.75mg/L, NNA 0.12mg/L, IBA 0.06mg/L.
The microcystic aeruginosa of taking the logarithm vegetative period is inoculated in this substratum and cultivates, illumination every day 8 hours, and the incubator temperature is controlled at about 25 degrees centigrade, the cell density of Microcystis aeruginosa in the per 3 days record substratum.
The substratum contrast experiment
Use BG11, DM, these three kinds of substratum of JM are substratum in contrast, compares with embodiment 1-4, and the result of contrast is as shown in the table:
Can find out from this table, use substratum provided by the present invention than using the contrast substratum can make more quickly Microcystis aeruginosa propagation, the time that reaches Microcystis aeruginosa density peak is faster 3 days than the substratum that uses contrast, and the maximum concentration that Microcystis aeruginosa can reach also is much higher than control medium.Illustrated that substratum provided by the invention has clear superiority.
In addition, can notice in the substratum of embodiment 1-3 and all added Mo (NO
3)
3.5H
2O, and do not add Mo (NO in the substratum among the embodiment 4
3)
3.5H
2O.From upper table, when being in the rise period, microcystic aeruginosa adds Mo (NO
3)
3.5H
2The O DeGrain.But we can see that by experiment therefore the speed of the Microcystis aeruginosa death after reaching the concentration peak of the Microcystis aeruginosa among the embodiment 4 add Mo (NO far away faster than embodiment 1-3
3)
3.5H
2O can make Microcystis aeruginosa keep the time of concentration peak level greatly to increase in substratum, can increase about 2 days.
For Mo (NO
3)
3.5H
2The interpolation metering of O, we have done following experiment.Directly get among the embodiment 4 the 9th day nutrient solution, add therein the Mo (NO of different meterings
3)
3.5H
2Then O continues to cultivate, thereby determines suitable Mo (NO
3)
3.5H
2The interpolation metering of O.Data by following table can be found out, Mo (NO
3)
3.5H
2Effect was best when the interpolation of O was measured in the scope of 2～3mg/L.
Those skilled in the art can make replacement or modification to content of the present invention according to content disclosed by the invention and the art technology of grasping; but these replacements or modification should not be considered as breaking away from the present invention's design, and these replacements or modification are all in the claimed interest field of the present invention.
Claims (2)
1. substratum that is used for Microcystis aeruginosa, the prescription of this substratum is as follows:
NaNO
31～1.2g/L，NH
4NO
3100～105mg/L，KNO
3450～455mg/L，CaCl
2.2H
2O500～510mg/L，MgSO
4.7H
2O 170～175mg/L，K
2HPO
4200～205mg/L，KI0.5～0.55mg/L，H
3BO
39.1～9.3mg/L，MnSO
4.4H
2O 20～20.5mg/L，ZnSO
4.7H
2O 5.3～5.5mg/L，CuSO
4.5H
2O 0.02～0.03mg/L，CoCl
2.6H
2O 0.02～0.03mg/L；
Ironic citrate 6～10mg/L, Na
2-EDTA.2H
2O 15～20mg/L, inositol 15～20mg/L, nicotinic acid 0.2～0.3mg/L, vitamins B
120.7～0.8mg/L, NNA 0.1～0.15mg/L, IBA 0.05～0.08mg/L.
2. the substratum that is used for Microcystis aeruginosa described in the claim 1 is characterized in that adding Mo (NO again in above-mentioned culture medium prescription
3)
3.5H
2O 2～3mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104357536A CN102978116A (en) | 2012-11-05 | 2012-11-05 | Microcystis culture medium formula |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104357536A CN102978116A (en) | 2012-11-05 | 2012-11-05 | Microcystis culture medium formula |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102978116A true CN102978116A (en) | 2013-03-20 |
Family
ID=47852458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012104357536A Pending CN102978116A (en) | 2012-11-05 | 2012-11-05 | Microcystis culture medium formula |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102978116A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563344A (en) * | 2004-03-22 | 2005-01-12 | 中国科学院水生生物研究所 | Method for cultivating micro capsule alga of producing microcysin in high yield |
| CN101955904A (en) * | 2010-10-25 | 2011-01-26 | 北京大学 | Algicidal bacteria separation method in natural water environment |
| CN102494986A (en) * | 2011-11-22 | 2012-06-13 | 南京大学 | Method for determining floating percentage of microcystis |
-
2012
- 2012-11-05 CN CN2012104357536A patent/CN102978116A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563344A (en) * | 2004-03-22 | 2005-01-12 | 中国科学院水生生物研究所 | Method for cultivating micro capsule alga of producing microcysin in high yield |
| CN101955904A (en) * | 2010-10-25 | 2011-01-26 | 北京大学 | Algicidal bacteria separation method in natural water environment |
| CN102494986A (en) * | 2011-11-22 | 2012-06-13 | 南京大学 | Method for determining floating percentage of microcystis |
Non-Patent Citations (1)
| Title |
|---|
| 卢蒙: "富营养化池塘中微囊藻毒素的生态学变化及毒素对植物生长的影响", 《中国优秀博硕士学位论文全文数据库（硕士） 农业科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104745513B (en) | One plant production PQQ Hyphomicrobium and its application | |
| CN102154170B (en) | Microcystin degrading strain and method for degrading MC-LR (microcystins-LR) by same | |
| CN101705243B (en) | Application of method for smearing and transforming agrobacterium rhizogene-mediated hypocotyl to soybean transformation | |
| Gibor | The culture of brine algae | |
| CN204291868U (en) | Artificial medium is adopted to carry out the locellus culture apparatus of bush mycorrhizal fungi pure culture | |
| CN104818236B (en) | A kind of Rhizosphere Soils in Tea Garden growth-promoting bacterial slime Serratieae and its application | |
| CN105284620A (en) | Method for rescuing grown-up seedlings of special precocious peach hybrid embryos | |
| CN106187505A (en) | A kind of Chinese cabbage growth-promoting root mill water culture nutrient solution and preparation method thereof | |
| Shukia et al. | Antarctic cyanobacteria as a source of phycocyanin: an assessment | |
| CN105543145B (en) | One plant of removing magnesium ion, phosphate anion and bacterium of ammonium ion and application thereof | |
| CN101748093B (en) | Streptomyces spectabilis SpLE-16 and application thereof to production of spectinomycin | |
| CN104862232B (en) | A kind of improved culture medium and compound method suitable for haematococcus pluvialis nutrient growth | |
| CN106635866A (en) | Thiobacillus thioparus and high-activity culture method | |
| CN102978116A (en) | Microcystis culture medium formula | |
| CN106676055A (en) | Method for reducing culture medium surface contamination of plant tissue culture | |
| CN106359085A (en) | E.urophylla*E.saligna rooting culture medium and application thereof | |
| Budinoff et al. | Ecophysiology of a Mono Lake picocyanobacterium | |
| CN102676444B (en) | Culture medium for promoting growth of bacillus and application thereof | |
| CN102978117A (en) | Chroococcus culture medium formula | |
| CN105368756B (en) | The bacterium and application thereof of one plant of removing calcium ion and magnesium ion | |
| CN106591138A (en) | Chlorella pyrenoidosa culture medium composition | |
| CN105199988B (en) | One plant of root telogenesis film bacterium RS2 with luxuriant and rich with fragrance degradation function and its application | |
| CN105217799A (en) | A kind of industrial fermentation method of molten algae streptomycete active substance | |
| CN112662583A (en) | Improved culture medium suitable for vegetative growth of nostoc sphaeroides and preparation method thereof | |
| CN106520662A (en) | Synthetic medium used for herba cistanche cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130320 |