CN102978104B - Power-free self-flowing type directed evolution device for hydrogen-production microorganisms - Google Patents
Power-free self-flowing type directed evolution device for hydrogen-production microorganisms Download PDFInfo
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- CN102978104B CN102978104B CN201210528498.XA CN201210528498A CN102978104B CN 102978104 B CN102978104 B CN 102978104B CN 201210528498 A CN201210528498 A CN 201210528498A CN 102978104 B CN102978104 B CN 102978104B
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Abstract
The invention discloses a power-free self-flowing type directed evolution device for hydrogen-production microorganisms, and belongs to the fields of environmental engineering and energy and chemical industry. The power-free self-flowing type directed evolution device is characterized in that reactors are customized; three holes are formed on caps of the reactors and are respectively connected with a water inlet pipe, a pressure gage and a water outlet pipe; sampling ports are formed at the bottoms of reacting bottles; and different reactors are connected by silicone hoses. According to the power-free self-flowing type directed evolution device for the hydrogen-production microorganisms, hydrogen production culture mediums with the same volume are filled into all reaction bottles during starting the hydrogen generation through anaerobe, wherein only the concentration of the carbon source substrates in the hydrogen production culture mediums is gradually increased, and hydrogen-production microorganism flora is inoculated in the first reactor. After the hydrogen production reaction is started, and the pressure of hydrogen in the reactors reaches a certain degree, the fermentation broth in the bottle is pressed into the following reactor, and the hydrogen production reaction liquid can sequentially flow in different reactors in the same way, so that the hydrogen production capacity of microorganisms is gradually improved.
Description
Technical field
The present invention relates to a kind of hydrogen microorganism orthogenesis device, particularly a kind of unpowered gravity flow of producing and produce hydrogen microorganism orthogenesis device.
Background technology
Along with rapid development of economy, environmental pollution and energy shortage have become the two large problems that affects current social development.Thereby, develop reproducible green energy resource extremely urgent, wherein hydrogen is high with its energy density, can be considered to one of optimal substitute energy by cleanburning.In the preparation method of various hydrogen, the advantage such as, environmental protection efficient because having is extensively concerned again for biological hydrogen production.Biological hydrogen production can be divided into anaerobism photosynthetic-hydrogen-production and anaerobically fermenting hydrogen manufacturing, compared with photosynthetic-hydrogen-production, anaerobically fermenting hydrogen production process have specific hydrogen production rate high, be not subject to the advantages such as light application time restriction, available organism scope be wide.Thereby, utilize the anaerobic hydrogen production technology that organic waste etc. is carbon source, can in reducing production costs, realize disposal of resources and the recovery energy of organic waste.
In anaerobe hydrogen production process, being accompanied by the organic acids such as acetic acid that the hydrolysis of carbon source in substrate, acidifying produce, butyric acid, propionic acid, lactic acid excessively accumulates, can make anaerobically fermenting system pH decline and will suppress to produce hydrogen microorganism active, so seriously restrict anaerobe hydrogen production process continue carry out.For this reason, people conventionally adopt and improve the design of producing hydrogen reactor, the parameters of H-H reaction is produced in regulation and control and optimization, and promote to produce the measures such as hydrogen substrate hydrolysis to improve hydrogen generation efficiency by the even biological method of physics, chemistry, but these methods can not be removed the inhibition that in bio-hydrogen process, Accumulation of Organic Acids causes microorganism at all.Therefore, how to improve the acid resistance that produces hydrogen microorganism, be more and more subject to the attention of all circles of industries, universities and research institutors from alleviating in essence or removing the caused feedback inhibition of Accumulation of Organic Acids.So far, not yet there is the report that can improve the device that produces hydrogen microorganism acid resistance.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of product hydrogen microorganism orthogenesis device of unpowered gravity flow, and this device produces the unpowered gravity flow of H-H reaction liquid in each stage reactor by the increase of hydrogen dividing potential drop to realize; Meanwhile, also by increasing step by step the concentration of producing carbon source in hydrogen substratum in each reactor, produce hydrogen microorganism to organic acid tolerance and hydrogen production potential to improve.
Unpowered gravity flow provided by the invention produces hydrogen microorganism orthogenesis device and is formed by connecting by silicone tube (10) by the independent product hydrogen reactor (1) of level Four, every grade is produced hydrogen reactor (1) and comprises a reaction flask (11), on its bottle cap (4), have three holes, connect respectively water inlet pipe (3), pressure warning unit (2) and rising pipe (6), reaction flask bottom has thief hole (9), each stage reactor is connected to a whole set of orthogenesis device by silicone tube (10), the product hydrogen substratum of different volumes is housed in each reactor reaction interval (5), in first step reactor, inoculation is produced hydrogen microorganism through anaerobic grain sludge (8) conduct of high-temperature sterilization.
In this device, pass into nitrogen to maintain anaerobic environment in reaction unit, and to keep hydrogen production bioreactor rotating speed by constant-temperature table be 110rpm, temperature is 37 ° of C.For realizing unpowered gravity flow process, in first step reactor, rising pipe is lower than producing hydrogen substratum liquid level, and in the second stage, the third stage, fourth stage reactor, rising pipe is all higher than producing hydrogen substratum liquid level.Reaction flask is that volume is the glass reaction bottle of 500mL customization, and reaction flask external diameter is 9cm, is highly 18cm, and bottleneck external diameter is 4cm, and described silicone tube external diameter is 7mm, and internal diameter is 4mm.
Beneficial effect of the present invention: as the unpowered gravity flow microorganism of one orthogenesis device, reaction unit of the present invention can and reach after certain pressure by the increase of hydrogen dividing potential drop in each reactor, promotes fermented liquid and flows between different reactor; Meanwhile, by improving step by step carbon source concentration of substrate in each reactor, more can tolerate higher concentration organic acid hydrogenogens to tame out, and then realize microorganism orthogenesis.
Brief description of the drawings
Fig. 1 is that the unpowered gravity flow of indication of the present invention produces hydrogen microorganism orthogenesis apparatus structure schematic diagram
1, produce hydrogen reactor; 2, pressure warning unit; 3, water inlet pipe; 4, perforate plastic bottle closure; 5, reaction zone; 6, rising pipe; 7, pressure warning unit (pH meter) interface; 8, anaerobic sludge (producing hydrogen microorganism); 9, thief hole; 10, silicone tube; 11, reaction flask Fig. 2 is hydrogen dividing potential drop distribution in each reactor in orthogenesis device
Fig. 3 is organic acid and hydrogen component content distribution in each stage reactor
Embodiment
1,2,3 be described as follows by reference to the accompanying drawings:
As shown in Figure 1, the present invention adopts by level Four product hydrogen reactor (A/B/C/D) and joins end to end and connect the microorganism orthogenesis device forming via silicone tube (10), wherein every A reactor is by water inlet pipe (3), pressure warning unit (2), rising pipe (6), pressure tap (7), water outlet (9) composition, wherein, except rising pipe import in first step reactor (A) there was not product hydrogen substratum, in remaining reaction device (B/C/D), rising pipe import is all higher than producing hydrogen substratum liquid level.
First, in A reactor reaction bottle, inoculate into 10% anaerobic grain sludge (8), and in 37 ° of C, under 110rpm reaction conditions, start device of the present invention.After product hydrogen process starts, as shown in Figure 2, when hydrogen dividing potential drop in first step reactor (A) runs up to after certain pressure, promotion is wherein produced to H-H reaction liquid and be pressed into next stage reactor second stage reactor (B); Secondly, in the fermented liquid due to inflow B reactor, contain and produce hydrogen microorganism, therefore will start the product hydrogen process of B reactor; In addition, less owing to comprising organic acid in the reaction solution flowing into and wherein produce hydrogen microbe population from A reactor, in B reactor, carbon source concentration is higher simultaneously, only have after the hydrogen dividing potential drop in B reactor reaches relevant pressure, just can promote wherein reaction solution and flow to next stage, thereby B reactor will form the product hydrogen microorganism species that organic acid is more had to tolerance producing in hydrogen process; In like manner, in C/D reactor, will carry out similar reaction process; Finally, as shown in Figure 3, from organic acid concentration and hydrogen component content distribution in the each stage reactor of A/B/C/D, produce after hydrogen end of processing, in B, C reactor, not only organic acid concentration is high compared with A, and in B reactor, hydrogen dividing potential drop is higher, thereby compared with remaining reaction device (A/D), can conclude that in B, C reactor, producing hydrogen microorganism has better tolerance to organic acid, thereby will there is higher product hydrogen performance.
In sum, adopt unpowered gravity flow of the present invention to produce hydrogen microorganism orthogenesis device, can in realization response device, produce significantly improving of hydrogen microorganism acid resistance and hydrogen production potential, use orienting device provided by the invention rapidly and efficiently to screen and produce hydrogen microorganism.
Be understandable that, for those of ordinary skills, can be equal to replacement or change according to technical scheme of the present invention and inventive concept thereof, and all these changes or replacement all should belong to the protection domain of the appended claim of the present invention.
Claims (4)
1. a unpowered gravity flow produces hydrogen microorganism orthogenesis device, it is characterized in that this device is formed by connecting by silicone tube (10) by the independent product hydrogen reactor (1) of level Four, every grade is produced hydrogen reactor (1) and comprises a reaction flask (11), on its bottle cap (4), have three holes, connect respectively water inlet pipe (3), pressure warning unit (2) and rising pipe (6), reaction flask bottom has thief hole (9), each stage reactor is connected to a whole set of orthogenesis device by silicone tube (10), the product hydrogen substratum of different volumes is housed in each reactor reaction interval (5), in first step reactor, inoculation is produced hydrogen microorganism through anaerobic grain sludge (8) conduct of high-temperature sterilization, in described first step reactor, rising pipe is lower than producing hydrogen substratum liquid level, in the second stage, the third stage, fourth stage reactor, rising pipe is all higher than producing hydrogen substratum liquid level.
2. product hydrogen microorganism orthogenesis device according to claim 1, is characterized in that passing into nitrogen to maintain anaerobic environment in reaction unit in this device, and to keep hydrogen production bioreactor rotating speed by constant-temperature table be 110rpm, and temperature is 37 DEG C.
3. product hydrogen microorganism orthogenesis device according to claim 1, is characterized in that reaction flask is that volume is the glass reaction bottle of 500mL customization, and reaction flask external diameter is 9cm, is highly 18cm, and bottleneck external diameter is 4cm.
4. product hydrogen microorganism orthogenesis device according to claim 1, is characterized in that described silicone tube external diameter is 7mm, and internal diameter is 4mm.
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| CN201210528498.XA CN102978104B (en) | 2012-12-10 | 2012-12-10 | Power-free self-flowing type directed evolution device for hydrogen-production microorganisms |
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| CN201210528498.XA CN102978104B (en) | 2012-12-10 | 2012-12-10 | Power-free self-flowing type directed evolution device for hydrogen-production microorganisms |
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| CN102978104B true CN102978104B (en) | 2014-09-17 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103771590A (en) * | 2014-02-24 | 2014-05-07 | 陈贤芳 | Method and device for processing sewage by virtue of multistage alternative microbial generator |
| CN112266848B (en) * | 2020-11-09 | 2022-08-02 | 河南农业大学 | Novel no pump formula inner loop formula photosynthetic biological hydrogen production reactor |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201224726Y (en) * | 2008-04-30 | 2009-04-22 | 昆明理工大学 | Biofermentation continuous hydrogen production plant |
| CN101831463A (en) * | 2010-06-04 | 2010-09-15 | 中国科学院青岛生物能源与过程研究所 | Method for preparing biogas by two-phase anaerobic fermentation of oil-extracted algae slag |
| CN101993896A (en) * | 2010-11-11 | 2011-03-30 | 江南大学 | Method for continuously producing hydrogen and polyhydroxyalkanoates by taking blue-green algae as substrate through coupling fermentation |
| CN102212837A (en) * | 2011-05-10 | 2011-10-12 | 北京博莱特威能源技术有限公司 | Hydrogen production technology and generation device with high efficiency |
| CN102765699A (en) * | 2012-08-22 | 2012-11-07 | 吴仁政 | Hydrogen generating system |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201224726Y (en) * | 2008-04-30 | 2009-04-22 | 昆明理工大学 | Biofermentation continuous hydrogen production plant |
| CN101831463A (en) * | 2010-06-04 | 2010-09-15 | 中国科学院青岛生物能源与过程研究所 | Method for preparing biogas by two-phase anaerobic fermentation of oil-extracted algae slag |
| CN101993896A (en) * | 2010-11-11 | 2011-03-30 | 江南大学 | Method for continuously producing hydrogen and polyhydroxyalkanoates by taking blue-green algae as substrate through coupling fermentation |
| CN102212837A (en) * | 2011-05-10 | 2011-10-12 | 北京博莱特威能源技术有限公司 | Hydrogen production technology and generation device with high efficiency |
| CN102765699A (en) * | 2012-08-22 | 2012-11-07 | 吴仁政 | Hydrogen generating system |
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