CN102973934B - Preparation method for Newcastle disease and avian influenza bi-combined inactivated vaccine - Google Patents
Preparation method for Newcastle disease and avian influenza bi-combined inactivated vaccine Download PDFInfo
- Publication number
- CN102973934B CN102973934B CN201210501862.3A CN201210501862A CN102973934B CN 102973934 B CN102973934 B CN 102973934B CN 201210501862 A CN201210501862 A CN 201210501862A CN 102973934 B CN102973934 B CN 102973934B
- Authority
- CN
- China
- Prior art keywords
- gallus domesticus
- embryo gallus
- deactivation
- avian influenza
- venom
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 206010064097 avian influenza Diseases 0.000 title claims abstract description 71
- 208000002979 Influenza in Birds Diseases 0.000 title claims abstract description 47
- 208000010359 Newcastle Disease Diseases 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 229940031551 inactivated vaccine Drugs 0.000 title claims abstract description 16
- 241000700605 Viruses Species 0.000 claims abstract description 69
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 238000011081 inoculation Methods 0.000 claims abstract description 38
- VEZXCJBBBCKRPI-UHFFFAOYSA-N beta-propiolactone Chemical compound O=C1CCO1 VEZXCJBBBCKRPI-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229960000380 propiolactone Drugs 0.000 claims abstract description 9
- 210000003837 chick embryo Anatomy 0.000 claims abstract description 7
- 210000001161 mammalian embryo Anatomy 0.000 claims description 77
- 241000287828 Gallus gallus Species 0.000 claims description 72
- 239000002435 venom Substances 0.000 claims description 62
- 231100000611 venom Toxicity 0.000 claims description 62
- 210000001048 venom Anatomy 0.000 claims description 62
- 230000009849 deactivation Effects 0.000 claims description 49
- 239000012141 concentrate Substances 0.000 claims description 22
- 231100000614 poison Toxicity 0.000 claims description 22
- 239000002574 poison Substances 0.000 claims description 22
- 238000011218 seed culture Methods 0.000 claims description 20
- 238000002156 mixing Methods 0.000 claims description 18
- 230000001954 sterilising effect Effects 0.000 claims description 18
- 230000003760 hair shine Effects 0.000 claims description 16
- 241000271566 Aves Species 0.000 claims description 14
- 230000003612 virological effect Effects 0.000 claims description 13
- 239000008346 aqueous phase Substances 0.000 claims description 12
- 238000001816 cooling Methods 0.000 claims description 12
- 230000001804 emulsifying effect Effects 0.000 claims description 12
- 206010014599 encephalitis Diseases 0.000 claims description 12
- 230000003203 everyday effect Effects 0.000 claims description 12
- 239000012071 phase Substances 0.000 claims description 12
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 12
- 229920000053 polysorbate 80 Polymers 0.000 claims description 12
- 230000004083 survival effect Effects 0.000 claims description 12
- 238000003760 magnetic stirring Methods 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- CEGOLXSVJUTHNZ-UHFFFAOYSA-K aluminium tristearate Chemical compound [Al+3].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O CEGOLXSVJUTHNZ-UHFFFAOYSA-K 0.000 claims description 6
- 230000007062 hydrolysis Effects 0.000 claims description 6
- 238000006460 hydrolysis reaction Methods 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 238000011068 loading method Methods 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000010792 warming Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 12
- 230000000937 inactivator Effects 0.000 abstract description 9
- 230000008569 process Effects 0.000 abstract description 4
- 238000011534 incubation Methods 0.000 abstract 2
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 230000002779 inactivation Effects 0.000 abstract 1
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 18
- 239000000427 antigen Substances 0.000 description 15
- 102000036639 antigens Human genes 0.000 description 15
- 108091007433 antigens Proteins 0.000 description 15
- 238000004519 manufacturing process Methods 0.000 description 14
- 229960000190 bacillus calmette–guérin vaccine Drugs 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 6
- 238000001914 filtration Methods 0.000 description 6
- 230000000415 inactivating effect Effects 0.000 description 6
- 230000001717 pathogenic effect Effects 0.000 description 4
- 238000007086 side reaction Methods 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- 229960005486 vaccine Drugs 0.000 description 3
- 206010067482 No adverse event Diseases 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 210000002345 respiratory system Anatomy 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 230000008719 thickening Effects 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 241000711404 Avian avulavirus 1 Species 0.000 description 1
- 101710132601 Capsid protein Proteins 0.000 description 1
- 101710094648 Coat protein Proteins 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 1
- 101710125418 Major capsid protein Proteins 0.000 description 1
- 101710141454 Nucleoprotein Proteins 0.000 description 1
- 101710083689 Probable capsid protein Proteins 0.000 description 1
- 208000019802 Sexually transmitted disease Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 208000031169 hemorrhagic disease Diseases 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000037797 influenza A Diseases 0.000 description 1
- 230000000622 irritating effect Effects 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Description
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210501862.3A CN102973934B (en) | 2012-11-29 | 2012-11-29 | Preparation method for Newcastle disease and avian influenza bi-combined inactivated vaccine |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210501862.3A CN102973934B (en) | 2012-11-29 | 2012-11-29 | Preparation method for Newcastle disease and avian influenza bi-combined inactivated vaccine |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102973934A CN102973934A (en) | 2013-03-20 |
CN102973934B true CN102973934B (en) | 2015-04-08 |
Family
ID=47848467
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201210501862.3A Active CN102973934B (en) | 2012-11-29 | 2012-11-29 | Preparation method for Newcastle disease and avian influenza bi-combined inactivated vaccine |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102973934B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104928259A (en) * | 2015-05-22 | 2015-09-23 | 杨凌绿方生物工程有限公司 | H9 subtype of avian influenza virus inactivating vaccine and preparation method thereof |
CN104906570A (en) * | 2015-05-22 | 2015-09-16 | 杨凌绿方生物工程有限公司 | Newcastle disease and H9 subtype bird flu combined inactivate vaccine and preparing method thereof |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101607084A (en) * | 2008-06-18 | 2009-12-23 | 洛阳普莱柯生物工程有限公司 | The preparation method of newcastle disease, H9 subtype avian influenza bivalent inactivated vaccine |
CN101491672A (en) * | 2009-02-24 | 2009-07-29 | 乾元浩生物股份有限公司 | Chicken new castle disease-bird influenza dual oil-emulsion inactivated vaccine and preparation method thereof |
CN102302775B (en) * | 2011-07-06 | 2013-06-05 | 普莱柯生物工程股份有限公司 | Combined inactivated vaccine of Newcastle disease and H9 subtype avian influenza and preparation method thereof |
-
2012
- 2012-11-29 CN CN201210501862.3A patent/CN102973934B/en active Active
Also Published As
Publication number | Publication date |
---|---|
CN102973934A (en) | 2013-03-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103374547B (en) | A kind of preparation method of foot and mouth disease purified vaccine | |
CN101612397B (en) | Duck virus hepatitis inactivated vaccine and preparation method thereof | |
CN104922663A (en) | New castle disease and H9 subtype bird flu bivalent vaccine | |
CN104946600B (en) | A kind of H9 subtype avian influenza virus strain | |
CN104162154A (en) | Bivalent inactivated vaccine against bovine viral diarrhea and infectious bovine rhinotracheitis, and preparation method and application thereof | |
CN106729690A (en) | The preparation method of newcastle disease, avian influenza virus and aviadenovirus triple inactivated vaccine | |
CN102178945B (en) | Preparation method of inactivated vaccine for iridovirus of grouper | |
CN106119212A (en) | Aviadenovirus strain, inactivated vaccine and preparation method | |
CN102727879B (en) | Spinner bottle production method of inactivated singapore grouper iridovirus (SGIV) vaccine | |
CN102038949A (en) | Method for producing newcastle disease, infectious bronchitis, egg drop syndrome and avian influenza (H9 subtype) combined inactivated vaccine | |
CN102973934B (en) | Preparation method for Newcastle disease and avian influenza bi-combined inactivated vaccine | |
CN104212771A (en) | Avian influenza virus proliferation method and avian influenza vaccine prepared by use of avian influenza virus proliferation method | |
CN102688485A (en) | Preparation method of porcine parvovirus inactivated vaccine | |
CN107050448A (en) | A kind of preparation method of avian influenza virus, aviadenovirus bivalent inactivated vaccine | |
CN104548088A (en) | Method for preparing bivalent vaccine of newcastle disease virus La Sota strain and infectious bronchitis virus N-S multi-epitope protein | |
CN103830724B (en) | Muscovy duck parvovirus inactivation vaccine and application thereof | |
CN101745106A (en) | Porcine parvnvirus living vaccine and preparation method thereof | |
CN102965344B (en) | Production of infectious bronchitis virus and vaccine from cell line | |
CN104725503A (en) | Fugu rubripes nervous necrosis virus capsid protein egg yolk antibody and application thereof | |
CN102886043B (en) | Binary inactivated vaccine against Japanese encephalitis virus and porcine parvovirus and preparation method thereof | |
CN106177938A (en) | Duck viral hepatitis bivalent inactivated vaccine and preparation method thereof | |
CN102716478A (en) | Combined Newcastle disease and infectious bronchitis inactivated vaccine and method for preparing same | |
CN104984337A (en) | Newcastle disease and avian flu antigen-antibody complex inactivated vaccine and preparation method | |
CN112143714B (en) | Method for producing H7 subtype avian influenza virus inactivated vaccine by using low-immunity chick embryo | |
CN112080478B (en) | Efficient propagation method and application of H5 subtype avian influenza virus |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C53 | Correction of patent of invention or patent application | ||
CB03 | Change of inventor or designer information |
Inventor after: Jiang Xinghua Inventor after: Lin Zhikun Inventor after: Chen Yuwen Inventor after: Lu Yibin Inventor before: Jiang Xinghua Inventor before: Lin Zhikun Inventor before: Chen Yuwen Inventor before: Chen Yibin |
|
COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: JIANG XINGHUA LIN ZHIKUN CHEN YUWEN CHEN YIBIN TO: JIANG XINGHUA LIN ZHIKUN CHEN YUWEN LU YIBIN |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CP01 | Change in the name or title of a patent holder | ||
CP01 | Change in the name or title of a patent holder |
Address after: Gushan Town Park in Jinan District of Fuzhou city in Fujian province village 110 350000 Patentee after: Zhaofenghua Biotechnology (Fuzhou) Co.,Ltd. Address before: Gushan Town Park in Jinan District of Fuzhou city in Fujian province village 110 350000 Patentee before: FUZHOU DA BEI NONG BIOTECH Co.,Ltd. |
|
TR01 | Transfer of patent right |
Effective date of registration: 20200618 Address after: 211102, 999 Ting Dong Road, Jiangning economic and Technological Development Zone, Nanjing, Jiangsu Patentee after: Zhaofenghua Biotechnology (Nanjing) Co.,Ltd. Address before: Gushan Town Park in Jinan District of Fuzhou city in Fujian province village 110 350000 Patentee before: Zhaofenghua Biotechnology (Fuzhou) Co.,Ltd. |
|
TR01 | Transfer of patent right |