CN102965406B - Biosynthesis method of cadmium sulfide quantum dot - Google Patents
Biosynthesis method of cadmium sulfide quantum dot Download PDFInfo
- Publication number
- CN102965406B CN102965406B CN201210524623.XA CN201210524623A CN102965406B CN 102965406 B CN102965406 B CN 102965406B CN 201210524623 A CN201210524623 A CN 201210524623A CN 102965406 B CN102965406 B CN 102965406B
- Authority
- CN
- China
- Prior art keywords
- quantum dot
- source
- mycelium pellet
- phanerochaete chrysosporium
- synthesis process
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Luminescent Compositions (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a Biosynthesis method of a cadmium sulfide quantum dot. The method comprises the following steps of: on the basis of taking thioacetamide as an S source, water soluble cadmium salt as a Cd source, a sulfhydryl compound as a stabilizer and a phanerochaete chrysosporium mycelium pellet as an adsorbent, adding the S source, the Cd source and the sulfhydryl compound into a culture solution which contains the phanerochaete chrysosporium mycelium pellet to carry out shake cultivation on a shaking table, and carrying out filtration, cleaning in deionized water, ultrasonication, centrifuging and purification after the cultivation to obtain the cadmium sulfide quantum dot. The method provided by the invention is simple in operation, low in cost and good in reproducibility; and the prepared cadmium sulfide quantum dot has the characteristics of low toxicity, good biocompatibility and excellent optical property.
Description
Technical field
The present invention relates to the preparation of nano material, be specifically related to a kind of biological synthesis process of cadmiumsulfide quantum dot.
Background technology
Quantum dot (quantum dots, QDs), claims again semiconductor nano, is elementary composition by II～VI family, III～V family, Si, Ge etc., and size with interior nano particle, has obvious quantum confined effect at its exciton Bohr radius.In nearly decades, quantum dot is owing to having unique electricity and optical property, in research fields such as biological chemistry, molecular biology and photocells, shown extremely wide application prospect, compare with traditional organic fluorescent dye, quantum dot has the adjustable fluorescent emission of size, narrow and symmetrical emmission spectrum, wide and continuous absorption spectrum and the fabulous advantages such as light stability, makes the synthetic method of quantum dot be subject to exploring more and more widely and studying.
Early stage quantum dot synthetic method mostly is metal organic phase method, even if organometallics is grown in having the organic solvent environment of Coordinate property.As 1993, the use dimethyl cadmiums such as Murray and trioctylphosphine selenizing phosphine are made precursor, it is injected to trioctyl phosphine oxide (the trioctylphosphine oxide of 350 ℃ of vigorous stirring successively, TOPO) in solution, the CdSe QDs that has synthesized high fluorescent yield, adopts size Selection intermediate processing can obtain monodispersed CdSe QDs.Quantum dot prepared by metal organic phase method has very outstanding advantage, as controlled in granularity, fluorescence emission peak is narrow etc., but the highly toxic chemicals of the many employings of metal organic phase method, as tri octyl phosphine and trioctylphosphine oxide (TOPO), synthetic quantum dot toxicity is large, and biocompatibility is low.The synthetic great majority of cadmiumsulfide quantum dot are to adopt chemical method at present, and chemical method has and itself has advantages of, such as effectively controlling size and the pattern of quantum dot.But also there are a lot of drawbacks in chemical method, as large in preparation toxicity used, expense is high, and great majority need at high temperature react, and the biocompatibility that makes quantum dot is not high.Hydrothermal synthesis method is the more method of using at present, and this method is simple to operate, cost is lower, but the monochromaticity of synthetic quantum dot is not good, peak width at half height is narrow not, and needs high temperature, 2005, Wang Yong first waits and adopts the synthetic bare quantum spot Cadmium Sulfide of hydrothermal method, and its peak width at half height is nearly 100nm.
In view of quantum dot has slowly stepped into the field of life science, synthetic toxicity quantum dot low, that biocompatibility is high and monochromaticity is good becomes a kind of active demand.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, provide a kind of simple to operate, preparation condition is gentle, the biological synthesis process of the cadmiumsulfide quantum dot of with low cost, favorable reproducibility, the standby cadmiumsulfide quantum dot toxicity of this legal system is low, biocompatibility is high and monochromaticity good.
In order to solve the problems of the technologies described above, the present invention proposes a kind of biological synthesis process of cadmiumsulfide quantum dot, described method is to take thioacetamide as S source, take water-soluble cadmium salt as Cd source, take sulfhydryl compound as stablizer, Phanerochaete chrysosporium (Phanerochaete chrysosporium) mycelium pellet of take is adsorbent, by described S source, Cd source and sulfhydryl compound add containing carrying out shaking table shaking culture in the nutrient solution of Phanerochaete chrysosporium mycelium pellet, cultivation completes by filtration, washed with de-ionized water, ultrasonication, centrifugal and purifying, obtain cadmiumsulfide quantum dot.
In aforesaid method, the ratio of the amount of substance of described S source, Cd source and sulfhydryl compound is 1: 2～10: 100～200.
In aforesaid method, described water-soluble cadmium salt is CdCl
2, Cd (NO
3)
24H
2o, Cd (ClO
4)
2, CdSO
4in a kind of.
In aforesaid method, described sulfhydryl compound is one or more in Cys, mercaptosuccinic acid, halfcystine, Thiovanic acid, thiohydracrylic acid, sulfydryl butyric acid, thioglycerin.
In aforesaid method, described nutrient solution is Kirk minimal media liquid, and its pH value is 9～12.
In aforesaid method, the culture temperature of described shaking table shaking culture is 30 ℃～40 ℃, and hunting speed is 120r/min～150r/min, and incubation time is 10h～15h.
In aforesaid method, the preparation of described Phanerochaete chrysosporium mycelium pellet comprises the following steps:
(1) from the conidium of Phanerochaete chrysosporium described in the surperficial scraping of nutrient agar, conidium is evenly suspended in distilled water, regulates turbidity, make to contain 10 in every milliliter of suspension
4～2.5 * 10
6individual conidium, obtains spore suspension;
(2) described spore suspension is inoculated in described nutrient solution with 1%～3% volume ratio, in temperature, be that 30 ℃～40 ℃, hunting speed are to carry out shaking table shaking culture under 120r/min～150r/min, the pH value condition that is 6.5～7.5, until form Phanerochaete chrysosporium mycelium pellet.
Compared with prior art, the invention has the advantages that: the present invention has utilized the synthetic technology of biological auxiliary absorption, simple to operate, preparation condition is gentle, with low cost, favorable reproducibility.What biological synthesis process of the present invention was introduced is the chemicals of low toxicity, thereby the cadmiumsulfide quantum dot of preparation has that toxicity is low, good biocompatibility, feature that stability is high.In addition, the cadmiumsulfide quantum dot geomery homogeneous that utilizes the present invention to prepare, monochromaticity good (peak width at half height is less than 20nm), has excellent optical property.
accompanying drawing explanation
Fig. 1 is the embodiment of the present invention 1 is respectively the CdS quantum dot of preparing under 9,10,11 condition ultraviolet-visible absorption spectroscopy figure in pH value.
Fig. 2 is the embodiment of the present invention 1 is respectively the CdS quantum dot of preparing under 9,10,11 condition fluorescence emission spectrogram in pH value.
Fig. 3 is that in the embodiment of the present invention 1, pH value is the transmission electron microscope photo of the supernatant liquor that contains equally distributed CdS quantum dot in the step (4) of 9 o'clock.
Embodiment
Below in conjunction with Figure of description and specific embodiment, the present invention is described in further detail.
embodiment 1:
A biological synthesis process for cadmiumsulfide quantum dot of the present invention, comprises the following steps:
(1) by the lip-deep Phanerochaete chrysosporium BKMF-1767(CCTCC of nutrient agar AF96007) conidium of (other bacterial strain of the same race is all applicable to the present invention) scrapes in distilled water, the turbidity to 60 that regulates suspension, makes to contain 10 in every milliliter of suspension
4～2.5 * 10
6individual conidium, forms spore suspension;
(2) 3ml spore suspension is inoculated in the Erlenmeyer flask of the 500ml capacity that 200mlKirk minimal media liquid is housed, in temperature, be that 37 ℃, hunting speed are that 150r/min, pH carry out shaking table shaking culture under 7.0 condition, after 3 days, in nutrient solution, automatically formed the Phanerochaete chrysosporium mycelium pellet of 2mm～3mm;
(3) to adding 10ml mass concentration in above-mentioned nutrient solution, be thioacetamide, the 0.138gCd (NO of 0.668g/l
3)
24H
2o and 1.325g Thiovanic acid, be adjusted to 9 by pH, and temperature is that 37 ℃, hunting speed are 150r/min, continues after shaking table shaking culture 12h, makes the mycelia absorption on mycelium pellet synthesize CdS quantum dot;
(4) with common filter paper filtering nutrient solution, collection obtains mycelium pellet, with washed with de-ionized water mycelium pellet three times, adopt cell Ultrasonic Cell Disruptor that mycelium pellet is carried out to ultrasonication, the CdS quantum dot that makes to be adsorbed on mycelium pellet is scattered in solution, after CdS quantum dot disperses, adopt whizzer centrifugal this solution under the rotating speed of 6000r/min, after 10min, at the bottom of broken thalline is sunken to the pipe of centrifuge tube, CdS quantum dot is dispersed in supernatant liquor, collects supernatant liquor, after purified, obtain CdS quantum dot, particle diameter is 2nm～5nm.
For contrasting, repeat aforesaid operations step, be only that the pH in step (3) is adjusted to respectively to 10 and 11, prepare the CdS quantum dot under condition of different pH.
Adopt ultraviolet-visual spectrometer to test above-mentioned pH and be respectively the CdS quantum dot of preparing under 9,10,11 condition, the absorption spectrum obtaining as shown in Figure 1.Adopt fluorescence emission spectrometer to test above-mentioned pH and be respectively the CdS quantum dot of preparing under 9,10,11 condition, excitation wavelength is 397nm, and the fluorescence emission spectrum obtaining as shown in Figure 2.From Fig. 1 and Fig. 2, the absorb light spectrum width of CdS quantum dot and continuously, fluorescence emission spectrum is narrow and symmetrical, and peak width at half height is less than 20nm, illustrates that thus the monochromaticity of CdS quantum dot is good, has excellent spectrum property.
Adopting pH in transmissioning electric mirror test above-mentioned steps (3) is the supernatant liquor that contains equally distributed CdS quantum dot in step (4) at 9 o'clock, and magnification ratio is 5,000,000 times.As shown in Figure 3, be that pH is the microscopic appearance of 9 o'clock CdS quantum dots, as can be seen from the figure, the lattice fringe of CdS quantum dot is very clear, and the diameter of sample is 2nm～5nm, and CdS quantum dot size homogeneous is described thus, has good crystalline network.
embodiment 2:
A biological synthesis process for cadmiumsulfide quantum dot of the present invention, comprises the following steps:
(1) by the lip-deep Phanerochaete chrysosporium BKMF-1767(CCTCC of nutrient agar AF96007) conidium scrape in distilled water, regulate the turbidity to 60 of suspension, make to contain 10 in every milliliter of suspension
4～2.5 * 10
6individual conidium, forms spore suspension;
(2) 3ml spore suspension is inoculated in the Erlenmeyer flask of the 500ml capacity that 200mlKirk minimal media liquid is housed, in temperature, be that 35 ℃, hunting speed are that 120r/min, pH carry out shaking table shaking culture under 7.0 condition, after 3 days, in nutrient solution, automatically formed the Phanerochaete chrysosporium mycelium pellet of 2mm～3mm;
(3) to adding 10ml mass concentration in above-mentioned nutrient solution, be thioacetamide, the 0.082gCdCl of 0.668g/l
2with 2.096g mercaptosuccinic acid, pH is adjusted to 9, temperature is that 35 ℃, hunting speed are 120r/min, continues after shaking table shaking culture 10h, makes the mycelia absorption on mycelium pellet synthesize CdS quantum dot;
(4) with common filter paper filtering nutrient solution, collection obtains mycelium pellet, with washed with de-ionized water mycelium pellet three times, adopt cell Ultrasonic Cell Disruptor that mycelium pellet is carried out to ultrasonication, the CdS quantum dot that makes to be adsorbed on mycelium pellet is scattered in solution, after CdS quantum dot disperses, adopt whizzer centrifugal this solution under the rotating speed of 6000r/min, after 10min, at the bottom of broken thalline is sunken to the pipe of centrifuge tube, CdS quantum dot is dispersed in supernatant liquor, collects supernatant liquor, obtains CdS quantum dot after purified.
embodiment 3:
A biological synthesis process for cadmiumsulfide quantum dot of the present invention, comprises the following steps:
(1) by the lip-deep Phanerochaete chrysosporium BKMF-1767(CCTCC of nutrient agar AF96007) conidium scrape in distilled water, regulate the turbidity to 60 of suspension, make to contain 10 in every milliliter of suspension
4～2.5 * 10
6individual conidium, forms spore suspension;
(2) 3ml spore suspension is inoculated in the Erlenmeyer flask of the 500ml capacity that 200mlKirk minimal media liquid is housed, in temperature, be that 37 ℃, hunting speed are that 150r/min, pH carry out shaking table shaking culture under 7.0 condition, after 3 days, in nutrient solution, automatically formed the Phanerochaete chrysosporium mycelium pellet of 2mm～3mm;
(3) to adding 10ml mass concentration in above-mentioned nutrient solution, be thioacetamide, the 0.139gCd (ClO of 0.668g/l
4)
2with 1.077g halfcystine, pH is adjusted to 10, temperature is that 37 ℃, hunting speed are 150r/min, continues after shaking table shaking culture 12h, makes the mycelia absorption on mycelium pellet synthesize CdS quantum dot;
(4) with common filter paper filtering nutrient solution, collection obtains mycelium pellet, with washed with de-ionized water mycelium pellet three times, adopt cell Ultrasonic Cell Disruptor that mycelium pellet is carried out to ultrasonication, the CdS quantum dot that makes to be adsorbed on mycelium pellet is scattered in solution, after CdS quantum dot disperses, adopt whizzer centrifugal this solution under the rotating speed of 6000r/min, after 10min, at the bottom of broken thalline is sunken to the pipe of centrifuge tube, CdS quantum dot is dispersed in supernatant liquor, collects supernatant liquor, obtains CdS quantum dot after purified.
embodiment 4:
A biological synthesis process for cadmiumsulfide quantum dot of the present invention, comprises the following steps:
(1) by the lip-deep Phanerochaete chrysosporium BKMF-1767(CCTCC of nutrient agar AF96007) conidium scrape in distilled water, regulate the turbidity to 60 of suspension, make to contain 10 in every milliliter of suspension
4～2.5 * 10
6individual conidium, forms spore suspension;
(2) 3ml spore suspension is inoculated in the Erlenmeyer flask of the 500ml capacity that 200mlKirk minimal media liquid is housed, in temperature, be that 30 ℃, hunting speed are that 150r/min, pH carry out shaking table shaking culture under 7.0 condition, after 3 days, in nutrient solution, automatically formed the Phanerochaete chrysosporium mycelium pellet of 2mm～3mm;
(3) to adding 10ml mass concentration in above-mentioned nutrient solution, be thioacetamide, the 0.093gCdSO of 0.668g/l
4with 1.132g thiohydracrylic acid, pH is adjusted to 11, temperature is that 30 ℃, hunting speed are 150r/min, continues after shaking table shaking culture 15h, makes the mycelia absorption on mycelium pellet synthesize CdS quantum dot;
(4) with common filter paper filtering nutrient solution, collection obtains mycelium pellet, with washed with de-ionized water mycelium pellet three times, adopt cell Ultrasonic Cell Disruptor that mycelium pellet is carried out to ultrasonication, the CdS quantum dot that makes to be adsorbed on mycelium pellet is scattered in solution, after CdS quantum dot disperses, adopt whizzer centrifugal this solution under the rotating speed of 6000r/min, after 10min, at the bottom of broken thalline is sunken to the pipe of centrifuge tube, CdS quantum dot is dispersed in supernatant liquor, collects supernatant liquor, obtains CdS quantum dot after purified.
embodiment 5:
A biological synthesis process for cadmiumsulfide quantum dot of the present invention, comprises the following steps:
(1) by the lip-deep Phanerochaete chrysosporium BKMF-1767(CCTCC of nutrient agar AF96007) conidium scrape in distilled water, regulate the turbidity to 60 of suspension, make to contain 10 in every milliliter of suspension
4～2.5 * 10
6individual conidium, forms spore suspension;
(2) 3ml spore suspension is inoculated in the Erlenmeyer flask of the 500ml capacity that 200mlKirk minimal media liquid is housed, in temperature, be that 37 ℃, hunting speed are that 120r/min, pH carry out shaking table shaking culture under 7.0 condition, after 3 days, in nutrient solution, automatically formed the Phanerochaete chrysosporium mycelium pellet of 2mm～3mm;
(3) to adding 10ml mass concentration in above-mentioned nutrient solution, be thioacetamide, the 0.138gCd (NO of 0.668g/l
3)
24H
2o and 1.616gL-halfcystine, be adjusted to 12 by pH, and keeping temperature is that 37 ℃, hunting speed are 120r/min, continues after shaking table shaking culture 12h, makes the mycelia absorption on mycelium pellet synthesize CdS quantum dot;
(4) with common filter paper filtering nutrient solution, collection obtains mycelium pellet, with washed with de-ionized water mycelium pellet three times, adopt cell Ultrasonic Cell Disruptor that mycelium pellet is carried out to ultrasonication, the CdS quantum dot that makes to be adsorbed on mycelium pellet is scattered in solution, after CdS quantum dot disperses, adopt whizzer centrifugal this solution under the rotating speed of 6000r/min, after 10min, at the bottom of broken thalline is sunken to the pipe of centrifuge tube, CdS quantum dot is dispersed in supernatant liquor, collects supernatant liquor, obtains CdS quantum dot after purified.
embodiment 6:
A biological synthesis process for cadmiumsulfide quantum dot of the present invention, comprises the following steps:
(1) by the lip-deep Phanerochaete chrysosporium BKMF-1767(CCTCC of nutrient agar AF96007) conidium scrape in distilled water, regulate the turbidity to 60 of suspension, make to contain 10 in every milliliter of suspension
4～2.5 * 10
6individual conidium, forms spore suspension;
(2) 3ml spore suspension is inoculated in the Erlenmeyer flask of the 500ml capacity that 200mlKirk minimal media liquid is housed, in temperature, be that 37 ℃, hunting speed are that 120r/min, pH carry out shaking table shaking culture under 7.0 condition, after 3 days, in nutrient solution, automatically formed the Phanerochaete chrysosporium mycelium pellet of 2mm～3mm;
(3) to adding 10ml mass concentration in above-mentioned nutrient solution, be thioacetamide, the 0.082gCdCl of 0.668g/l
2with 1.510g thiohydracrylic acid, pH is adjusted to 10, keeping temperature is that 37 ℃, hunting speed are 120r/min, continues after shaking table shaking culture 10h, makes the mycelia absorption on mycelium pellet synthesize CdS quantum dot;
(4) with common filter paper filtering nutrient solution, collection obtains mycelium pellet, with washed with de-ionized water mycelium pellet three times, adopt cell Ultrasonic Cell Disruptor that mycelium pellet is carried out to ultrasonication, the CdS quantum dot that makes to be adsorbed on mycelium pellet is scattered in solution, after CdS quantum dot disperses, adopt whizzer centrifugal this solution under the rotating speed of 6000r/min, after 10min, at the bottom of broken thalline is sunken to the pipe of centrifuge tube, CdS quantum dot is dispersed in supernatant liquor, collects supernatant liquor, obtains CdS quantum dot after purified.
embodiment 7:
A biological synthesis process for cadmiumsulfide quantum dot of the present invention, comprises the following steps:
(1) by the lip-deep Phanerochaete chrysosporium BKMF-1767(CCTCC of nutrient agar AF96007) conidium scrape in distilled water, regulate the turbidity to 60 of suspension, make to contain 10 in every milliliter of suspension
4～2.5 * 10
6individual conidium, forms spore suspension;
(2) 3ml spore suspension is inoculated in the Erlenmeyer flask of the 500ml capacity that 200mlKirk minimal media liquid is housed, in temperature, be that 35 ℃, hunting speed are that 130r/min, pH carry out shaking table shaking culture under 7.0 condition, after 3 days, in nutrient solution, automatically formed the Phanerochaete chrysosporium mycelium pellet of 2mm～3mm;
(3) to adding 10ml mass concentration in nutrient solution, be thioacetamide, the 0.093gCdSO of 0.668g/l
4with 1.325g Thiovanic acid, pH is adjusted to 11, keeping temperature is that 35 ℃, hunting speed are 130r/min, continues after shaking table shaking culture 12h, makes the mycelia absorption on mycelium pellet synthesize CdS quantum dot;
(4) with common filter paper filtering nutrient solution, collection obtains mycelium pellet, with washed with de-ionized water mycelium pellet three times, adopt cell Ultrasonic Cell Disruptor that mycelium pellet is carried out to ultrasonication, the CdS quantum dot that makes to be adsorbed on mycelium pellet is scattered in solution, after CdS quantum dot disperses, adopt whizzer centrifugal this solution under the rotating speed of 6000r/min, after 10min, at the bottom of broken thalline is sunken to the pipe of centrifuge tube, CdS quantum dot is dispersed in supernatant liquor, collects supernatant liquor, obtains CdS quantum dot after purified.
The above is only the preferred embodiment of the present invention, and protection scope of the present invention is also not only confined to above-described embodiment, and all technical schemes belonging under thinking of the present invention all belong to protection scope of the present invention.Should propose, for those skilled in the art, improvements and modifications without departing from the principles of the present invention, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (4)
1. the biological synthesis process of a cadmiumsulfide quantum dot, it is characterized in that, described biological synthesis process is to take thioacetamide as S source, take water-soluble cadmium salt as Cd source, take sulfhydryl compound as stablizer, take Phanerochaete chrysosporium mycelium pellet as adsorbent, described S source, Cd source and sulfhydryl compound are added containing carrying out shaking table shaking culture in the nutrient solution of Phanerochaete chrysosporium mycelium pellet, cultivated by filtration, washed with de-ionized water, ultrasonication, centrifugal and purifying and obtained cadmiumsulfide quantum dot;
The ratio of the amount of substance of described S source, Cd source and sulfhydryl compound is 1: 2～10: 100～200;
Described nutrient solution is Kirk minimal media liquid, and its pH value is 9～12;
The culture temperature of described shaking table shaking culture is 30 ℃～40 ℃, and hunting speed is 120r/min～150r/min, and incubation time is 10h～15h.
2. the biological synthesis process of cadmiumsulfide quantum dot according to claim 1, is characterized in that, described water-soluble cadmium salt is CdCl
2, Cd (NO
3)
24H
2o, Cd (ClO
4)
2, CdSO
4in a kind of.
3. the biological synthesis process of cadmiumsulfide quantum dot according to claim 1, it is characterized in that, described sulfhydryl compound is one or more in Cys, mercaptosuccinic acid, halfcystine, Thiovanic acid, thiohydracrylic acid, sulfydryl butyric acid, thioglycerin.
4. the biological synthesis process of cadmiumsulfide quantum dot according to claim 1, is characterized in that, the preparation of described Phanerochaete chrysosporium mycelium pellet comprises the following steps:
(1) from the conidium of Phanerochaete chrysosporium described in the surperficial scraping of nutrient agar, conidium is evenly suspended in distilled water, regulates turbidity, make to contain 10 in every milliliter of suspension
4～2.5 * 10
6individual conidium, obtains spore suspension;
(2) described spore suspension is inoculated in described nutrient solution with 1%～3% volume ratio, in temperature, be that 30 ℃～40 ℃, hunting speed are to carry out shaking table shaking culture under 120r/min～150r/min, the pH value condition that is 6.5～7.5, until form Phanerochaete chrysosporium mycelium pellet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210524623.XA CN102965406B (en) | 2012-12-10 | 2012-12-10 | Biosynthesis method of cadmium sulfide quantum dot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210524623.XA CN102965406B (en) | 2012-12-10 | 2012-12-10 | Biosynthesis method of cadmium sulfide quantum dot |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102965406A CN102965406A (en) | 2013-03-13 |
| CN102965406B true CN102965406B (en) | 2014-04-02 |
Family
ID=47795842
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210524623.XA Expired - Fee Related CN102965406B (en) | 2012-12-10 | 2012-12-10 | Biosynthesis method of cadmium sulfide quantum dot |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102965406B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103316692A (en) * | 2013-06-24 | 2013-09-25 | 江苏大学 | Preparation method and application of CdS/CNTs composite photocatalyst |
| CN104911215A (en) * | 2015-05-28 | 2015-09-16 | 中国科学院海洋研究所 | Method for synthesizing CdS quantum dots based on microorganism metabolic activity |
| CN107574139B (en) * | 2017-08-16 | 2020-06-12 | 湖南大学 | Method for relieving biological toxicity of CdSe/ZnS quantum dots by cysteine |
| CN108866104B (en) * | 2018-04-16 | 2021-10-01 | 中山大学附属第五医院 | Biosynthesis method of cadmium sulfide quantum dots, prepared cadmium sulfide quantum dots and application thereof |
| CN112973794A (en) * | 2021-03-08 | 2021-06-18 | 中国科学技术大学 | tetrahymena-CdS quantum dot intracellular hybridization system, construction method thereof and application thereof in visible light catalysis |
| CN113249246B (en) * | 2021-04-02 | 2022-09-13 | 江苏大学 | Cadmium sulfide quantum dot/clostridium thermocellum hybrid system and construction method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096687A (en) * | 2007-07-11 | 2008-01-02 | 武汉大学 | Method for preparing cadmium selenide quantum dots by using microbial cells |
| CN102134579A (en) * | 2010-12-31 | 2011-07-27 | 杭州师范大学 | Biosynthesizing method of cadmium telluride quantum dots |
-
2012
- 2012-12-10 CN CN201210524623.XA patent/CN102965406B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096687A (en) * | 2007-07-11 | 2008-01-02 | 武汉大学 | Method for preparing cadmium selenide quantum dots by using microbial cells |
| CN102134579A (en) * | 2010-12-31 | 2011-07-27 | 杭州师范大学 | Biosynthesizing method of cadmium telluride quantum dots |
Non-Patent Citations (6)
| Title |
|---|
| Heavy Metal Removal by Biosorption Using Phanerochaete chrysosporium;M.Gopal, et al.;《Applied Biochemistry and Biotechnology》;20021231;第102-103卷(第1-6期);第227-236页 * |
| M.Gopal, et al..Heavy Metal Removal by Biosorption Using Phanerochaete chrysosporium.《Applied Biochemistry and Biotechnology》.2002,第102-103卷(第1-6期),第227-236页. |
| 张晓蓉.微生物应用于纳米生物合成技术研究进展.《微生物学报》.2011,第51卷(第3期),第297-第304页. |
| 微生物应用于纳米生物合成技术研究进展;张晓蓉;《微生物学报》;20110304;第51卷(第3期);第297-第304页 * |
| 柠檬酸稳定的水溶性CdSe和CdSe/CdS量子点的荧光特性;邓大伟等;《无机化学学报》;20080510;第24卷(第5期);第701-707页 * |
| 邓大伟等.柠檬酸稳定的水溶性CdSe和CdSe/CdS量子点的荧光特性.《无机化学学报》.2008,第24卷(第5期),第701-707页. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102965406A (en) | 2013-03-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102965406B (en) | Biosynthesis method of cadmium sulfide quantum dot | |
| Wang et al. | Vertically aligned ZnO nanowire arrays tip-grafted with silver nanoparticles for photoelectrochemical applications | |
| Boelee et al. | Nitrogen and phosphorus removal from municipal wastewater effluent using microalgal biofilms | |
| Bhadwal et al. | Biogenic synthesis and photocatalytic activity of CdS nanoparticles | |
| Lam et al. | High-sensitive ultraviolet photodetectors based on ZnO nanorods/CdS heterostructures | |
| CN105883909B (en) | A kind of CsPbBrxI3‑xThe preparation method of nanometer rods | |
| CN110237802A (en) | Bismuth-bismuth tungstate-bismuth oxybromide ternary complex and its preparation method and application | |
| CN103241959A (en) | Preparation method of Na-doped ZnO nanorod array | |
| CN101747890B (en) | Synthesis and purification method of quantum dots wrapped by different ligands and product thereof | |
| Chang et al. | CuSZnS1− xOx/g-C3N4 heterostructured photocatalysts for efficient photocatalytic hydrogen production | |
| Li et al. | Co-assembly of photosystem II in nanotubular indium–tin oxide multilayer films templated by cellulose substance for photocurrent generation | |
| Yang et al. | Synthesis, growth mechanism and photocatalytic property of CdS with different kinds of surfactants | |
| Ridwan et al. | Fabrication of dye-sensitized solar cell using chlorophylls pigment from sargassum | |
| Fu et al. | Morphology‐and Size‐Controlled Fabrication of CdS from Flower‐Like to Spherical Structures and their Application for High‐Performance Photoactivity | |
| CN103897700B (en) | The preparation method of the CdTe quantum that a kind of Cys/thiohydracrylic acid is modified | |
| Noorasid et al. | Synthesis of ZnO nanorod using hydrothermal technique for dye-sensitized solar cell application | |
| Wang et al. | Seeded growth of ZnO nanowires in dye-containing solution: The submerged plant analogy and its application in photodegradation of dye pollutants | |
| Shen et al. | Large scale synthesis of stable tricolor Zn 1− x Cd x Se core/multishell nanocrystals via a facile phosphine-free colloidal method | |
| CN110803710B (en) | Method for preparing zinc oxide material based on surfactant-free microemulsion | |
| CN108037104A (en) | Oil content of microalgae quick determination method based on fat-soluble quantum dot | |
| CN109705862A (en) | Nanocrystal and preparation method thereof | |
| CN106206828A (en) | A kind of self assembly nucleocapsid SnO2the preparation method of ultraviolet detector | |
| CN107311217B (en) | Magnetic/fluorescent dual-functional EuCl2Eucl of nanocrystalline and superlattice structure2Nanocrystal | |
| Gautam et al. | Surfactant assisted self-assembly of zinc 5, 10-bis (4-pyridyl)-15, 20-bis (4-octadecyloxyphenyl) porphyrin into supramolecular nanoarchitectures | |
| Li et al. | Structural, optical, photocurrent and mechanism-induced photocatalytic properties of surface-modified ZnS thin films by chemical bath deposition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140402 Termination date: 20141210 |
|
| EXPY | Termination of patent right or utility model |