CN102954907A - Method for preparing color development reagent for detecting ligustrazine spots - Google Patents
Method for preparing color development reagent for detecting ligustrazine spots Download PDFInfo
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- CN102954907A CN102954907A CN 201110255139 CN201110255139A CN102954907A CN 102954907 A CN102954907 A CN 102954907A CN 201110255139 CN201110255139 CN 201110255139 CN 201110255139 A CN201110255139 A CN 201110255139A CN 102954907 A CN102954907 A CN 102954907A
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- China
- Prior art keywords
- reagent
- solid
- add
- weighing
- ligustrazine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- FINHMKGKINIASC-UHFFFAOYSA-N Tetramethylpyrazine Chemical compound CC1=NC(C)=C(C)N=C1C FINHMKGKINIASC-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000007787 solid Substances 0.000 claims abstract description 18
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims abstract description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 12
- 238000005303 weighing Methods 0.000 claims abstract description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 8
- QGWDKKHSDXWPET-UHFFFAOYSA-E pentabismuth;oxygen(2-);nonahydroxide;tetranitrate Chemical compound [OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[O-2].[Bi+3].[Bi+3].[Bi+3].[Bi+3].[Bi+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O QGWDKKHSDXWPET-UHFFFAOYSA-E 0.000 claims abstract description 8
- 229960000583 acetic acid Drugs 0.000 claims abstract description 4
- 239000012362 glacial acetic acid Substances 0.000 claims abstract description 4
- RXPAJWPEYBDXOG-UHFFFAOYSA-N hydron;methyl 4-methoxypyridine-2-carboxylate;chloride Chemical compound Cl.COC(=O)C1=CC(OC)=CC=N1 RXPAJWPEYBDXOG-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- 238000003828 vacuum filtration Methods 0.000 claims abstract description 4
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 230000001376 precipitating effect Effects 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 239000000052 vinegar Substances 0.000 abstract description 22
- 235000021419 vinegar Nutrition 0.000 abstract description 22
- 239000013049 sediment Substances 0.000 abstract 4
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 235000011837 pasties Nutrition 0.000 abstract 1
- 229910052709 silver Inorganic materials 0.000 abstract 1
- 239000004332 silver Substances 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 2
- 238000002211 ultraviolet spectrum Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000002537 thrombolytic effect Effects 0.000 description 1
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a method for preparing a color development reagent for detecting ligustrazine spots. The method comprises two steps of: 1, weighing 1.0 gram of bismuth nitrate solid, adding enough amount of water to dissolve the solid, adding more than 15 mL of 0.2 mol/L sodium hydroxide solution, separating out a large amount of silver pasty sediments from the solution, filtering, cleaning the sediments by using pure water, and carrying out vacuum filtration on the sediments to dry the sediments as much as possible, thus obtaining a basic bismuth nitrate solid; and 2, weighing 8.0 grams of potassium iodide solid, adding enough amount of pure water to dissolve the solid to be in constant volume of 20 mL, weighing 0.85 gram of the basic bismuth nitrate solid, sequentially adding 10 mL of glacial acetic acid, 40 mL of water and 20 mL of potassium iodide, and uniformly stirring to obtain a Dragendoff reagent, wherein the Dragendoff reagent stored in darkness at room temperature can be used for a long time. During use, 1 mL of the Dragendoff reagent is measured, and 2 mL of 0.6 mol/L hydrochloric acid solution is added; and the Dragendoff reagent and the hydrochloric acid solution are uniformly mixed, and water is added to be in the volume to 10 mL, so that the color development agent is prepared for standby use. The color development agent is suitable for quick detection of ligustrazine in vinegar, so that the quality of the vinegar can be quickly determined.
Description
Technical field
The invention belongs to a kind of preparation method of developer, be specifically related to a kind of method that detects the ligustrazine location reagent for preparing.
Technical background
Vinegar is that production history is long, widely used important flavouring.Make vinegar and already belong to the traditional handicraft industry, because historical reasons, the less stable of edible vinegar quality, and some lawless persons mingle in vinegar or produce false vinegar for reaping staggering profits in recent years, fill in proper order excellent, pass a fake product off as a genuine one, this has not only reduced product quality, and the infringement consumer gets interests, and seriously endangers the healthy of the people.The quality standard of existing vinegar is fairly simple, and the method for commonly using on the market is exactly to differentiate the true and false of vinegar according to information such as the physical and chemical index of prescribed by standard and external packings, but the difficult quality quality of judging accordingly vinegar.Also there is research to think by reappearance, stability and specificity analyses to the vinegar in ultraviolet spectrum curve, can obtain vinegar in ultraviolet spectrum and have its fingerprint characteristic, the fast detecting that can effectively be used for the vinegar kind, but this technical requirement personnel equipment and instrument is with high content of technology, and corresponding testing cost also increases thereupon.Ligustrazine is as one of health-care components important in the vinegar, have the pressure-lowering and blood-activing silt, improve coronary heart disease, thrombolysis presses down bolt, remove the health-care effect of free radical, and relative safety is higher, is one of main effective constituent of chuanxiong.In up-to-date Shanxi mature vinegar quality standard, strengthened in the physical and chemical index requirement to ligustrazine.Wherein, require in the Shanxi mature vinegar ligustrazine content more than or equal to 40mg/mL in the Shanxi mature vinegar physical and chemical index.There is complex procedures in the preparation method of traditional ligustrazine developer, and preparation cost is high, can not differentiate fast the defective of vinegar quality quality.
Summary of the invention
The present invention is exactly that the preparation method that will solve traditional ligustrazine developer exists complex procedures, and preparation cost is high, can not differentiate fast the problem of vinegar quality quality.
Technical scheme of the present invention: a kind of method that detects the ligustrazine location reagent for preparing is prepared in two steps:
1. take by weighing bismuth nitrate solid 1.0g, add the dissolving of capacity pure water, add again more than the 0.2mol/L sodium hydroxide solution 15mL, have a large amount of silvery white starchiness Precipitations in the solution this moment, filter and with pure water washing and precipitating thing, then the moisture during vacuum filtration will precipitate is drained as far as possible, obtains the basic bismuth nitrate solid
2. take by weighing potassium iodide solid 8.0g, add the dissolving of capacity pure water and be settled to 20mL, take by weighing above-mentioned basic bismuth nitrate solid 0.85g, add successively glacial acetic acid 10 mL, water 40 mL, above-mentioned liquor kalii iodide 20 mL and stir, obtain Dragendorff's reagent (Dragendoff reagent), the lucifuge room temperature preservation can be used for a long time.Measure Dragendorff's reagent 1 mL during use, add 0.6 mol/L hydrochloric acid solution, 2 mL, add water to 10 mL volumes behind the mixing, be mixed with developer stand-by.
Developer of the present invention is applicable to the fast detecting of ligustrazine in the vinegar, and specificity of the present invention is good, and is easy and simple to handle, easy to carry, and the easy interpretation of result is cheap, is applicable to the fast detecting of ligustrazine in the vinegar.Thereby can differentiate fast the quality of vinegar.
Embodiment:
1, a kind of method that detects the ligustrazine location reagent for preparing is prepared in two steps:
1. take by weighing bismuth nitrate solid 1.0g, add the dissolving of capacity pure water, add again more than the 0.2mol/L sodium hydroxide solution 15mL, have a large amount of silvery white starchiness Precipitations in the solution this moment, filter and with pure water washing and precipitating thing, then the moisture during vacuum filtration will precipitate is drained as far as possible, obtains the basic bismuth nitrate solid.
2. take by weighing potassium iodide solid 8.0g, add the dissolving of capacity pure water and be settled to 20mL, take by weighing above-mentioned basic bismuth nitrate solid 0.85g, add successively glacial acetic acid 10 mL, water 40 mL, above-mentioned liquor kalii iodide 20 mL and stir, obtain Dragendorff's reagent (Dragendoff reagent), the lucifuge room temperature preservation can be used for a long time.Measure Dragendorff's reagent 1 mL during use, add 0.6 mol/L hydrochloric acid solution, 2 mL, add water to 10 mL volumes behind the mixing, be mixed with developer.
Claims (1)
1. one kind prepares the method that detects the ligustrazine location reagent, it is characterized in that: be prepared in two steps:
1. take by weighing bismuth nitrate solid 1.0g, add the dissolving of capacity pure water, add again more than the 0.2mol/L sodium hydroxide solution 15mL, have a large amount of silvery white starchiness Precipitations in the solution this moment, filter and with pure water washing and precipitating thing, then the moisture during vacuum filtration will precipitate is drained as far as possible, obtains the basic bismuth nitrate solid
2. take by weighing potassium iodide solid 8.0g, add the dissolving of capacity pure water and be settled to 20mL, take by weighing above-mentioned basic bismuth nitrate solid 0.85g, add successively glacial acetic acid 10 mL, water 40 mL, above-mentioned liquor kalii iodide 20 mL and stir, obtain Dragendorff's reagent (Dragendoff reagent), the lucifuge room temperature preservation can be used for a long time, measure Dragendorff's reagent 1 mL during use, add 0.6 mol/L hydrochloric acid solution, 2 mL, add water to 10 mL volumes behind the mixing, be mixed with developer stand-by.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110255139 CN102954907A (en) | 2011-08-31 | 2011-08-31 | Method for preparing color development reagent for detecting ligustrazine spots |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110255139 CN102954907A (en) | 2011-08-31 | 2011-08-31 | Method for preparing color development reagent for detecting ligustrazine spots |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102954907A true CN102954907A (en) | 2013-03-06 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110255139 Pending CN102954907A (en) | 2011-08-31 | 2011-08-31 | Method for preparing color development reagent for detecting ligustrazine spots |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102954907A (en) |
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2011
- 2011-08-31 CN CN 201110255139 patent/CN102954907A/en active Pending
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130306 |