CN102944641A - Method for detecting medicinal material of Ranunculaceae plant radix paeoniae alba - Google Patents
Method for detecting medicinal material of Ranunculaceae plant radix paeoniae alba Download PDFInfo
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- CN102944641A CN102944641A CN2012105188785A CN201210518878A CN102944641A CN 102944641 A CN102944641 A CN 102944641A CN 2012105188785 A CN2012105188785 A CN 2012105188785A CN 201210518878 A CN201210518878 A CN 201210518878A CN 102944641 A CN102944641 A CN 102944641A
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Abstract
The invention belongs to the field of medicinal material identification and particularly relates to a method for detecting a medicinal material of Ranunculaceae plant radix paeoniae alba. According to the detection method, paeoniflorin is taken as a reference substance and thin layer chromatography is adopted for qualitative detection. The method comprises the following specific steps: firstly taking dried radix of paeoniae alba, grinding the radix, and passing the radix through a 200-mesh sieve; taking 2g of ground dried radix of paeoniae alba, carrying out ultrasonic treatment for 10-20 minutes after adding 30ml of extraction buffer solution, and carrying out centrifugation to obtain filtrate used as a test solution; adding the paeoniflorin reference substance with chloroform to prepare a solution with 1mg in per ml, wherein the solution is used as a reference substance solution; and respectively dropping 3-7Mul of test sample and 1Mul of reference sample on the same aluminum substrate, carrying out development by taking chloroform-ethyl acetate-methanol-methanoic acid as a developing solvent, taking out to dry the substrate in the air, spraying a vanilline sulfuric acid solution, and increasing temperature until spots occur and show the same color in the sunshine. The detection method is simple and feasible, strong in stability and low in cost.
Description
Technical field
The invention belongs to the evaluation field of medicinal material, relate to the qualitative analysis of effective constituent natural in the medicinal material, be specially a kind of detection method of ranunculaceae plant RADIX PAEONIAE ALBA medicinal material.
Background technology
The root of herbaceous peony is a kind of of Chinese herbaceous peony, the Paeonia per nnial herb, and high 40-70cm is without hair.Root manure is large, spindle or cylindrical, pitchy.Stem is upright, and top branch, base portion have the several pieces of membranous scales of sheath shape.The leaf alternate; Petiole reaches 9cm, is positioned at stem top person's petiole shorter; The lower portion of the stem leaf is two times trifoliolate leaves, and upper leaf is trifoliolate leaf; Narrow avette, the ellipse of leaflet or lanceolar, long 7.5-12cm, wide 2-4cm, tip be point gradually, base portion wedge shape or deflection, edge tool white hyaloidin serration, the two sides is without hair, and the below dredges along vein and gives birth to pubescence, nearly keratin.The flower both sexes, several living stems push up and axil diameter 7-12cm; Bract 4-5, lanceolar differs in size; Sepal 4, width egg shape or subcircular, long 1-1.5cm, wide 1-1.7cm, green, deposit the place; Petal 9-13, obovate, long 3.5-6cm, wide 1.5-4.5cm, white, sometimes base portion tool darkviolet patch or pink, the of all kinds and tool polyphyll of cultivation product petal; Stamen is most, the long 7-12mm of filigree, and flower pesticide is yellow; Floral disc tazza shape, parcel carpel base portion, the blunt circle of tip sliver; Carpel 2-5 is from life, without hair.Avette or the oval of Follicle radish fruit, long 2.5-3cm, diameter 1.2-1.5cm, tip tool rafter, the florescence 5-6 month, the fruit phase 6-8 month.The root of herbaceous peony can be used for treating postpartum vim and vigour makes a psychological attack, stomachache, and the side of body rib that also can be used for irritability discord is had a pain, or irritability takes advantage of the epigastric pain of spleen, reaches brothers' contraction and has a pain etc.
Summary of the invention
The objective of the invention is for above technical matters, provide to reduce detecting step, specificity is stronger, the detection method of the obvious a kind of ranunculaceae plant RADIX PAEONIAE ALBA medicinal material of testing result.
Concrete technical scheme of the present invention is as follows:
A kind of detection method of ranunculaceae plant RADIX PAEONIAE ALBA medicinal material, this detection method adopt thin-layered chromatography to carry out qualitative detection take Paeoniflorin as reference substance, and detection method may further comprise the steps:
(1) gets the dry root of cohosh Chinese herbaceous peony, then pulverize, pulverized 200 mesh sieves;
(2) get the powder 2g of the dry root of Chinese herbaceous peony after the pulverizing, add the quality percentage composition and be 45% methanol solution as Extraction buffer 30ml, then carry out ultrasonic processing 10-20min, then carry out the centrifugal filtrate that obtains, with filtrate as need testing solution; The speed of the hydro-extractor of its centrifugal employing is 3500r/min
(3) preparation of control sample solution: get the Paeoniflorin reference substance, chlorination is copied into the solution that every 1ml contains 1mg, in contrast product solution;
Getting thin-layered chromatography identifies: get test sample 3-7 μ l, control sample 1 μ l, put respectively on same aluminium substrate, chloroform-ethyl acetate take volume ratio as 30:4:8:0.2-methyl alcohol-formic acid is developping agent, launches, and takes out, dry, the vanillic aldehyde sulfuric acid solution of spray take the quality percentage composition as 3%-6% heated to the spot appearance, is placed on aobvious identical bluish violet spot under the daylight.
Good effect of the present invention is embodied in: detection method is simple, stability is stronger, and is with low cost, and operability is stronger, and testing result is obviously outstanding, is convenient to observe, reappearance is strong.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, the present invention is described in further detail below in conjunction with embodiment, but this should be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following embodiment.
Embodiment 1:
The detection method of gelsemium section plant RADIX PAEONIAE ALBA medicinal material take Paeoniflorin as reference substance, adopts thin-layered chromatography to carry out qualitative detection, and concrete steps are: get first the dry root of cohosh Chinese herbaceous peony, then pulverize, pulverized 200 mesh sieves; Then get the powder 2g of the dry root of Chinese herbaceous peony after the pulverizing, add the quality percentage composition and be 45% methanol solution as Extraction buffer 30ml, then carry out ultrasonic processing 15min, then carry out the centrifugal filtrate that obtains, with filtrate as need testing solution; The speed of the hydro-extractor of its centrifugal employing is 3500r/min, gets the Paeoniflorin reference substance, and chlorination is copied into the solution that every 1ml contains 1mg, in contrast product solution; Get test sample 5 μ l, control sample 1 μ l, put respectively on same aluminium substrate, chloroform-ethyl acetate take volume ratio as 30:4:8:0.2-methyl alcohol-formic acid is developping agent, launches, and takes out, dry, spray is take the vanillic aldehyde sulfuric acid solution of quality percentage composition as 4%, heats to spot to occur, and is placed on aobvious identical bluish violet spot under the daylight.
Embodiment 2:
The detection method of gelsemium section plant RADIX PAEONIAE ALBA medicinal material take Paeoniflorin as reference substance, adopts thin-layered chromatography to carry out qualitative detection, and concrete steps are: get first the dry root of cohosh Chinese herbaceous peony, then pulverize, pulverized 200 mesh sieves; Then get the powder 2g of the dry root of Chinese herbaceous peony after the pulverizing, add the quality percentage composition and be 45% methanol solution as Extraction buffer 30ml, then carry out ultrasonic processing 18min, then carry out the centrifugal filtrate that obtains, with filtrate as need testing solution; The speed of the hydro-extractor of its centrifugal employing is 3500r/min, gets the Paeoniflorin reference substance, and chlorination is copied into the solution that every 1ml contains 1mg, in contrast product solution; Get test sample 5 μ l, control sample 1 μ l, put respectively on same aluminium substrate, chloroform-ethyl acetate take volume ratio as 30:4:8:0.2-methyl alcohol-formic acid is developping agent, launches, and takes out, dry, spray is take the vanillic aldehyde sulfuric acid solution of quality percentage composition as 4%, heats to spot to occur, and is placed on aobvious identical bluish violet spot under the daylight.
Embodiment 3:
The dry root of the dry root of sample thief rose white, the dry root of white peony and the root of herbaceous peony is all crossed 200 mesh sieves, respectively get again each 2g of its powder, add respectively the quality percentage composition and be 45% methanol solution as Extraction buffer 30ml, then carry out ultrasonic processing 16min, then in the hydro-extractor of 3500r/min, carry out the centrifugal filtrate that obtains, filtrate as need testing solution, is got the Paeoniflorin reference substance, chlorination is copied into the solution that every 1ml contains 1mg, in contrast product solution;
Get test sample 5 μ l, control sample 1 μ l, put respectively on same aluminium substrate, chloroform-ethyl acetate take volume ratio as 30:4:8:0.2-methyl alcohol-formic acid is developping agent, launch, take out, dry, spray is take the vanillic aldehyde sulfuric acid solution of quality percentage composition as 4%, heat to spot and occur, be placed under the daylight and develop the color, in the sample, the root of herbaceous peony shows identical bluish violet with control sample, prove that it is the ripening fruits of gelsemium section plant RADIX PAEONIAE ALBA medicinal material, rose white, white peony is not under equal conditions all developed the color after this same operation, proves that namely it is not the ripening fruits of gelsemium section plant RADIX PAEONIAE ALBA medicinal material.
In the control sample that adopts among the embodiment 1 to embodiment 3, the purity of Paeoniflorin is greater than 95%.
Claims (7)
1. the detection method of a ranunculaceae plant RADIX PAEONIAE ALBA medicinal material is characterized in that this detection method take Paeoniflorin as reference substance, adopts thin-layered chromatography to carry out qualitative detection.
2. the detection method of RADIX PAEONIAE ALBA medicinal material according to claim 1 is characterized in that may further comprise the steps:
Get the dry root of cohosh Chinese herbaceous peony, then pulverize, pulverized 200 mesh sieves;
Get the powder 2g of the dry root of Chinese herbaceous peony after the pulverizing, add Extraction buffer 30ml, then carry out ultrasonic processing 10-20min, then carry out the centrifugal filtrate that obtains, with filtrate as need testing solution;
The preparation of control sample solution: get the Paeoniflorin reference substance, chlorination is copied into the solution that every 1ml contains 1mg, in contrast product solution;
Getting thin-layered chromatography identifies: get test sample 3-7 μ l, control sample 1 μ l puts respectively on same aluminium substrate, take chloroform-ethyl acetate-methyl alcohol-formic acid as developping agent, launch, take out, dry, spray is heated to the spot appearance with the vanillic aldehyde sulfuric acid solution, is placed on aobvious same color under the daylight.
3. the detection method of RADIX PAEONIAE ALBA medicinal material according to claim 2, it is characterized in that: the developping agent chloroform-ethyl acetate described in the step (4)-methyl alcohol-formic acid volume ratio is 30:4:8:0.2.
4. the detection method of RADIX PAEONIAE ALBA medicinal material according to claim 2, it is characterized in that: the Extraction buffer described in the step (2) refers to methanol solution, its quality percentage composition is 45%.
5. the detection method of RADIX PAEONIAE ALBA medicinal material according to claim 2 is characterized in that: centrifugal described in the step (2) obtains filtrate, and the speed of the hydro-extractor of its centrifugal employing is 3500r/min.
6. the detection method of RADIX PAEONIAE ALBA medicinal material according to claim 2 is characterized in that: be placed on the identical color of demonstration under the daylight lamp described in the step (4), be specially and show the bluish violet spot.
7. the detection method of RADIX PAEONIAE ALBA medicinal material according to claim 2 is characterized in that: step (4)
Described in the quality percentage composition of vanillic aldehyde sulfuric acid solution be 3%-6%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109541099A (en) * | 2018-11-27 | 2019-03-29 | 山东省食品药品检验研究院 | A kind of discrimination method of Radix Paeoniae Alba rhizome or its extract |
| CN113759067A (en) * | 2021-08-06 | 2021-12-07 | 北京康仁堂药业有限公司 | Thin-layer chromatography construction method, identification method and application thereof |
Citations (3)
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| CN1895378A (en) * | 2006-06-16 | 2007-01-17 | 贵州益佰制药股份有限公司 | Quality control for aqueous donkey-hide gelatin and Chinese angelica preparation |
| JP2011033591A (en) * | 2009-08-06 | 2011-02-17 | Naraken Chusho Kigyo Sien Center | Peony grade identification method |
| JP2011174899A (en) * | 2010-02-26 | 2011-09-08 | Naraken Chusho Kigyo Sien Center | Method for judging quality of peony |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1895378A (en) * | 2006-06-16 | 2007-01-17 | 贵州益佰制药股份有限公司 | Quality control for aqueous donkey-hide gelatin and Chinese angelica preparation |
| JP2011033591A (en) * | 2009-08-06 | 2011-02-17 | Naraken Chusho Kigyo Sien Center | Peony grade identification method |
| JP2011174899A (en) * | 2010-02-26 | 2011-09-08 | Naraken Chusho Kigyo Sien Center | Method for judging quality of peony |
Non-Patent Citations (2)
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| 国家药典委员会: "《中华人民共和国药典2000年版一部》", 31 January 2000 * |
| 王瑞: "川赤芍化学成分与芍药质量控制方法研究", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109541099A (en) * | 2018-11-27 | 2019-03-29 | 山东省食品药品检验研究院 | A kind of discrimination method of Radix Paeoniae Alba rhizome or its extract |
| CN113759067A (en) * | 2021-08-06 | 2021-12-07 | 北京康仁堂药业有限公司 | Thin-layer chromatography construction method, identification method and application thereof |
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Application publication date: 20130227 |