CN102944460A - Gel silver dyeing box for gel test - Google Patents
Gel silver dyeing box for gel test Download PDFInfo
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- CN102944460A CN102944460A CN2012104905723A CN201210490572A CN102944460A CN 102944460 A CN102944460 A CN 102944460A CN 2012104905723 A CN2012104905723 A CN 2012104905723A CN 201210490572 A CN201210490572 A CN 201210490572A CN 102944460 A CN102944460 A CN 102944460A
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Abstract
The invention relates to a gel silver dyeing box for gel test, and aims to solve the problems that liquid needs to be repeatedly fed and discharged in gel dyeing test, a gel glass plate needs to be manually picked and placed and the inner wall of a box body needs to be cleaned before liquid medicines are changed, time and labor are consumed, gel is quite easily damaged when taken out of the liquid medicines, and a certain probability of injury to people in the process of pouring the liquid medicines is caused. The gel silver dyeing box comprises a box body, electric push-pull rods, a gel tray, a direct-flow water suction pump, a liquid discharging pipe, a liquid medicine recycling bottle, a leaching guide pipe, a liquid feeding pipe, another liquid discharging pipe and a plurality of sprayers, the leaching guide pipe is provided with the sprayers, the gel tray is arranged in the box body, the electric push-pull rods, a timer and the leaching guide pipe are mounted on the box body, the liquid feeding pipe is hermetically connected with a hole and is connected with the direct-flow water suction pump, the former liquid discharging pipe is arranged at the bottom of the box body, and one end of the former liquid discharging pipe is inserted into a bottle opening of the liquid medicine recycling bottle. The gel silver dyeing box is used for the gel test.
Description
Technical field
The present invention relates to a kind of gel silver and dye case, be specifically related to a kind of gel silver for gel test and dye case.
Background technology
SSR and AFLP molecular marking technique have been widely used in fields such as genetic map construction, analysis of genetic diversity, and the related article delivered of 2010 to 2012 Academic Periodicals just has 2130 pieces (statistics is from middle National IP Networks) according to incompletely statistics.Polyacrylamide gel electrophoresis is the important means that detects oligonucleotides (such as SSR, AFLP), and silver-colored dyeing technique is a kind of DNA colouring method that is widely used, and its sensitivity is very high, and concentration limit is 0.0001ug/mm
2, namely reaching the level of nanogram, the level that detects with radioactive isotope is close, can directly not do analysis so need not concentrate just the lower sample of some concentration.Silver dyes experimentation and comprises a plurality of steps such as cleaning, fixing, cleaning, dyeing, cleaning, development, there are the following problems in the operation: 1. each step operating personnel all need change liquid, and change dressings and frontly all need clean cabinet wall, require great effort very much, 2. each step operating personnel all needs to topple over liquid, spills easily and spatters, the people is caused injury, 3. each step operation glass plate of all needing operating personnel will adhere to gel takes out from liquid, not only wastes time and energy, and very easily damages gel.
Summary of the invention
The present invention needs repeatedly feed liquor and discharge opeing in order to solve in gel-colored test, change dressings and all need pick and place manually the gel glass plate and clean cabinet wall before the liquid, waste time and energy, gel taken out from liquid very easily damage gel, and in toppling over the liquid process, the people there is the problem of the injury of certain probability, and then provides a kind of gel silver for gel test to dye case.
The present invention addresses the above problem the technical scheme that adopts to be: described gel silver is dyed case and is comprised casing, electric push rod, gel pallet, direct current suction pump, discharging tube, liquid returnable bottle, drip washing conduit, feed tube, discharging tube and a plurality of shower nozzle; Be processed with a plurality of the first holes of evenly arranging on the gel pallet, the pull bar external part of electric push rod is fixedly connected with a side of gel pallet, discharging tube is body, the drip washing conduit is a square framework, one end of square framework has the second hole, drip washing conduit framework inboard evenly is equipped with a plurality of shower nozzles, and a plurality of shower nozzle all is communicated with the drip washing conduit
Gel pallet is arranged in the casing, electric push rod is fixedly mounted on the side plate of casing one side, the drip washing conduit is installed near on the madial wall at casing top, one end and second hole of feed tube are tightly connected, the outlet end of the other end of feed tube and direct current suction pump is tightly connected, and the liquid-in pipe of direct current suction pump inserts in the distillation water source, and the bottom of casing is provided with discharging tube, and the bottom of casing is communicated with discharging tube, and an end of discharging tube inserts in the bottleneck of liquid returnable bottle.
The invention has the beneficial effects as follows: the present invention can realize automatic watering and discharge opeing, need not the operator and topples over liquid, and is time saving and energy saving, avoids dangerous, can improve the efficient of work; The present invention can realize by electric push rod the automatic lifting of gel dish, has both saved manpower, also farthest avoids damaging gel; The present invention can carry out drip washing to casing by the shower nozzle that the minisize dc self-priming water pump is transported to distilled water the drip washing conduit, greatly saved manpower, make experimental implementation more convenient, therefore use apparatus of the present invention and will greatly alleviate experimenter's muscle power input, improve conventional efficient and security, simple in structure, the satisfied high characteristics of requirement, convenient operation, usability that design that the present invention also has.
Description of drawings
Fig. 1 is one-piece construction synoptic diagram of the present invention, Fig. 2 is the structural representation of box cover of the present invention, Fig. 3 is the structural representation that electric push rod of the present invention is installed in gel pallet, and Fig. 4 is the structural representation that box cover of the present invention, the timer with quarter-bell, discharging tube, drip washing conduit and shower nozzle are installed in box house.
Embodiment
Embodiment one: present embodiment is described in conjunction with Fig. 1-Fig. 4, a kind of gel silver for gel test is dyed case, and described gel silver is dyed case and comprised casing 1, electric push rod 3, gel pallet 4, direct current suction pump 6, discharging tube 7, liquid returnable bottle 8, drip washing conduit 9, feed tube 11, discharging tube 12 and a plurality of shower nozzle 10; Be processed with a plurality of the first hole 4-1 that evenly arrange on the gel pallet 4, the pull bar external part of electric push rod 3 is fixedly connected with a side of gel pallet 4, discharging tube 7 is body, drip washing conduit 9 is a square framework, one end of square framework has the second hole 9-1, drip washing conduit 9 framework inboards evenly are equipped with a plurality of shower nozzles 10, and a plurality of shower nozzle 10 all is communicated with drip washing conduit 9
Gel silver is dyed step and need be cleaned, fixing, clean, dyeing, clean, the steps such as development, utilize the present invention only to need gel is put into gel pallet 4, finish rear the taking-up wait developing, picking and placeing gel glass plate number of times has reduced 10 times, the experiment of one clotting glue can be saved manpower 83.3%, the muscle power that has greatly alleviated the experimenter drops into, improve conventional efficient, utilize the present invention can realize automatically cleaning and automatically advancing discharge opeing, so that cause the probability of injury almost nil owing to topple over liquid to the people, promote the security of experiment, utilized liquid returnable bottle of the present invention that first round liquid is recovered in the corresponding bottle, can realize easily the utilization of liquid.
Embodiment two: present embodiment is described in conjunction with Fig. 1 and Fig. 4, a kind of gel silver for gel test is dyed case, described gel silver is dyed case and is also comprised timer 5 with quarter-bell, timer 5 with quarter-bell is installed on the sidewall of casing 1, utilize the timer 5 with quarter-bell among the present invention can remind the experimenter that certain step finishes, can greatly alleviate the burden that the experimenter records a plurality of step required times, improve conventional efficient, other is identical with embodiment one.
Embodiment three: present embodiment is described in conjunction with Fig. 1, Fig. 2 and Fig. 4, a kind of gel silver for gel test is dyed case, described gel silver is dyed case and is also comprised box cover 2, be processed with respectively two bayonet socket 2-1 on the dual-side of box cover 2, box cover 2 is arranged on the casing 1, prevent that the liquid in the casing from splashing to the casing outside, other is identical with embodiment one.
Embodiment four: present embodiment is described in conjunction with Fig. 1 and Fig. 4, a kind of gel silver for gel test is dyed case, described gel silver is dyed case and is also comprised feed tube joint 12, and an end of feed tube 11 is tightly connected with hole 9-1 by feed tube joint 12, and other is identical with embodiment one.
Embodiment five: present embodiment is described in conjunction with Fig. 1 and Fig. 3, a kind of gel silver for gel test is dyed case, there is the gap of 10mm-15mm distance on four limits of the gel pallet 4 respectively madial wall corresponding with casing 1, and other is identical with embodiment one.
Embodiment six: present embodiment is described in conjunction with Fig. 1 and Fig. 3, a kind of gel silver for gel test is dyed case, described gel pallet 4 is that rectangle is tabular, the length of gel pallet 4 is 450mm, wide is 400mm, the span of the diameter of hole 4-1 is 8mm-10mm on the gel pallet 4, and other is identical with embodiment five.
Embodiment seven: present embodiment is described in conjunction with Fig. 1, Fig. 3 and Fig. 4, a kind of gel silver for gel test is dyed case, described electric push rod 3 is two, two the electric push rod 3 pull bar external parts respectively dual-side relative with gel pallet 4 are fixedly connected with, two electric push rods 3 are installed in respectively on the relative two side of casing 1, and two electric push rods 3 are separately positioned in the bayonet socket 2-1, electric push rod 3 has been realized the automatic lifting of gel dish, both saved manpower, also farthest avoid damaging gel, other is identical with embodiment six.
Principle of work
In test gel silver being dyed case places on the shaking table, the latch of two electric push rods connects power supply, after electrophoresis finishes, the upper groove damping fluid in electrophoresis hole is poured out, take off the electrophoresis glass plate, gently glass plate is pried open to remove with scuppit and scribbled the recessed glass plate of peeling off silane, the glass plate gel that then will adhere to gel is put in the gel pallet facing up, and the switch of pressing electric push rod drops to bottom half with gel pallet, open the switch of automatic cleaning system, distilled water namely sprays from the shower nozzle of drip washing conduit, gel pallet is cleaned 2 times, with the immobile liquid (glacial acetic acid of 1% quality that has prepared in advance, the absolute ethyl alcohol of 10% quality) joins in the liquid holding box, by electric push rod pallet is fallen, set required time at the timer with quarter-bell, sound and can the prompting experimenter is fixing finish with the timer of the quarter-bell quarter-bell that makes zero, then, immobile liquid is entered the liquid returnable bottle from discharging tube, clean casing and gel pallet, by electric push rod gel pallet is lifted, with the dyeing liquor (silver nitrate of 0.2% quality that has prepared in advance, the glacial acetic acid of 1% quality, the absolute ethyl alcohol of 10% quality) adds in the casing, by electric push rod gel pallet is fallen and to be dyeed, after dyeing is finished, by electric push rod gel pallet is lifted, the discharging tube that will dye enters in the liquid returnable bottle, then clean casing and gel pallet 2 times, with developer solution (the anhydrous Na OH of 3% quality that has prepared in advance, the formaldehyde of 0.5% quality) join in the liquid holding box, by electric push rod gel pallet is fallen, carry out development treatment, after finishing developer solution is entered in the liquid returnable bottle by discharging tube, then clean casing, by electric push rod gel pallet is lifted, go out offset plate with the special gel gripping, and the offset plate after will developing is put on the lamp box, with Digital Video gel taken pictures.
Claims (7)
1. a gel silver that is used for gel test is dyed case, and it is characterized in that: described gel silver is dyed case and comprised casing (1), electric push rod (3), gel pallet (4), direct current suction pump (6), discharging tube (7), liquid returnable bottle (8), drip washing conduit (9), feed tube (11), discharging tube (12) and a plurality of shower nozzle (10); Gel pallet, (4) be processed with a plurality of the first holes of evenly arranging on, (4-1), electric push rod, (3) pull bar external part and gel pallet, (4) a side is fixedly connected with, discharging tube, (7) be body, the drip washing conduit, (9) be a square framework, one end of square framework has the second hole, (9-1), the drip washing conduit, (9) the framework inboard evenly is equipped with a plurality of shower nozzles, (10), and a plurality of shower nozzles, (10) all with the drip washing conduit, (9) be communicated with
Gel pallet (4) is arranged in the casing (1), electric push rod (3) is fixedly mounted on the side plate of casing 1 one sides, drip washing conduit (9) is installed near on the madial wall at casing (1) top, one end of feed tube (11) and the second hole (9-1) are tightly connected, the outlet end of the other end of feed tube (11) and direct current suction pump (6) is tightly connected, the liquid-in pipe of direct current suction pump (6) inserts in the distillation water source, the bottom of casing (1) is provided with discharging tube (7), and the bottom of casing (1) is communicated with discharging tube (7), and an end of discharging tube (7) inserts in the bottleneck of liquid returnable bottle (8).
2. described a kind of gel silver for gel test is dyed case according to claim 1, it is characterized in that: described gel silver is dyed case and is also comprised timer (5) with quarter-bell, is installed on the sidewall of casing (1) with the timer (5) of quarter-bell.
3. described a kind of gel silver for gel test is dyed case according to claim 1, it is characterized in that: described gel silver is dyed case and is also comprised box cover (2), be processed with respectively two bayonet sockets (2-1) on the dual-side of box cover (2), box cover (2) is arranged on the casing (1).
4. described a kind of gel silver for gel test is dyed case according to claim 1, it is characterized in that: described gel silver is dyed case and is also comprised feed tube joint (12), and an end of feed tube (11) is tightly connected by feed tube joint (12) and the second hole (9-1).
5. described a kind of gel silver for gel test is dyed case according to claim 1, it is characterized in that: there is the gap of 10mm-15mm distance on four limits of gel pallet (4) the respectively madial wall corresponding with casing (1).
6. described a kind of gel silver for gel test is dyed case according to claim 5, it is characterized in that: described gel pallet (4) is tabular for rectangle, the length of gel pallet (4) is 450mm, wide is 400mm, and the span of the diameter in the first hole (4-1) on the gel pallet (4) is 8mm-10mm.
7. described a kind of gel silver for gel test is dyed case according to claim 6, it is characterized in that: described electric push rod (3) is two, two electric push rods (3) the pull bar external part respectively dual-side relative with gel pallet (4) is fixedly connected with, two electric push rods (3) are installed in respectively on the relative two side of casing (1), and two electric push rods (3) are separately positioned in the bayonet socket (2-1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104905723A CN102944460A (en) | 2012-11-27 | 2012-11-27 | Gel silver dyeing box for gel test |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104905723A CN102944460A (en) | 2012-11-27 | 2012-11-27 | Gel silver dyeing box for gel test |
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| CN102944460A true CN102944460A (en) | 2013-02-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2012104905723A Pending CN102944460A (en) | 2012-11-27 | 2012-11-27 | Gel silver dyeing box for gel test |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103308369A (en) * | 2013-05-07 | 2013-09-18 | 华中农业大学 | Full-automatic silver staining developing instrument |
| CN103575584A (en) * | 2013-08-12 | 2014-02-12 | 北京林业大学 | Gel electrophoresis dyeing device |
| CN108760449A (en) * | 2018-07-17 | 2018-11-06 | 暨南大学 | The double-deck running gel dyeing apparatus, automation running gel dyeing apparatus and application method |
| CN108871914A (en) * | 2018-09-10 | 2018-11-23 | 河北省农林科学院粮油作物研究所 | A kind of post-processing approach and device of polyacrylamide gel electrophoresis product |
| CN113567219A (en) * | 2021-07-22 | 2021-10-29 | 甘肃农业大学 | Protein gel electrophoresis dyeing device and method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62167431A (en) * | 1985-11-30 | 1987-07-23 | Shimadzu Corp | Gel dyeing apparatus |
| JPH07120367A (en) * | 1993-10-21 | 1995-05-12 | J T Sci:Kk | Dyeing tank |
| CN2586964Y (en) * | 2002-11-27 | 2003-11-19 | 重庆医科大学 | Slide specimen dyeing machine |
| CN202119665U (en) * | 2011-07-12 | 2012-01-18 | 申允德 | Full-automatic gel dyeing instrument |
| CN202337743U (en) * | 2011-12-15 | 2012-07-18 | 齐齐哈尔大学 | Silver staining device for polyacrylamide gel |
| CN202420935U (en) * | 2012-01-13 | 2012-09-05 | 唐超 | Dual-purpose polyacrylamide gel electrophoresis dyeing and decolorizing tank |
-
2012
- 2012-11-27 CN CN2012104905723A patent/CN102944460A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62167431A (en) * | 1985-11-30 | 1987-07-23 | Shimadzu Corp | Gel dyeing apparatus |
| JPH07120367A (en) * | 1993-10-21 | 1995-05-12 | J T Sci:Kk | Dyeing tank |
| CN2586964Y (en) * | 2002-11-27 | 2003-11-19 | 重庆医科大学 | Slide specimen dyeing machine |
| CN202119665U (en) * | 2011-07-12 | 2012-01-18 | 申允德 | Full-automatic gel dyeing instrument |
| CN202337743U (en) * | 2011-12-15 | 2012-07-18 | 齐齐哈尔大学 | Silver staining device for polyacrylamide gel |
| CN202420935U (en) * | 2012-01-13 | 2012-09-05 | 唐超 | Dual-purpose polyacrylamide gel electrophoresis dyeing and decolorizing tank |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103308369A (en) * | 2013-05-07 | 2013-09-18 | 华中农业大学 | Full-automatic silver staining developing instrument |
| CN103575584A (en) * | 2013-08-12 | 2014-02-12 | 北京林业大学 | Gel electrophoresis dyeing device |
| CN103575584B (en) * | 2013-08-12 | 2016-02-03 | 北京林业大学 | A kind of gel electrophoresis dyeing apparatus |
| CN108760449A (en) * | 2018-07-17 | 2018-11-06 | 暨南大学 | The double-deck running gel dyeing apparatus, automation running gel dyeing apparatus and application method |
| CN108871914A (en) * | 2018-09-10 | 2018-11-23 | 河北省农林科学院粮油作物研究所 | A kind of post-processing approach and device of polyacrylamide gel electrophoresis product |
| CN113567219A (en) * | 2021-07-22 | 2021-10-29 | 甘肃农业大学 | Protein gel electrophoresis dyeing device and method |
| CN113567219B (en) * | 2021-07-22 | 2024-08-06 | 甘肃农业大学 | Protein gel electrophoresis staining device and method |
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Application publication date: 20130227 |