CN102943065A - Cell strain of giant cell tumor of human bone and application thereof - Google Patents
Cell strain of giant cell tumor of human bone and application thereof Download PDFInfo
- Publication number
- CN102943065A CN102943065A CN2012104675859A CN201210467585A CN102943065A CN 102943065 A CN102943065 A CN 102943065A CN 2012104675859 A CN2012104675859 A CN 2012104675859A CN 201210467585 A CN201210467585 A CN 201210467585A CN 102943065 A CN102943065 A CN 102943065A
- Authority
- CN
- China
- Prior art keywords
- bone
- cell tumor
- giant cell
- strain
- giant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a cell strain of a giant cell tumor of a human bone and application thereof. The cell strain of the giant cell tumor of the human bone is GCT-WFCCTCCNO. C2012101, and can be applied at the cellular level for study of mechanisms of generation, development and transfer of the giant cell tumor of the bone, an animal model of the giant cell tumor can be established, and the cell strain can be used as a target screening anticancer drug.
Description
Technical field
The present invention relates to a kind of cell strain, relate in particular to the strain of a kind of people's bone giant cell tumor and application thereof.
Background technology
Giant cell tumor of bone (giant cell tumor of bone is called for short GCT) is a kind of common primary bone tumor, accounts for 20% of former bone tumor.It is generally acknowledged that it belongs to optimum, once was called as " optimum giant cell tumor of bone (Blood-good) ".But by but easily recurrence after the innocent tumour ordinary method treatment, it is placed in the position between good, pernicious in recent years, belongs to intersexes (Intermediate) or aggressive (Aggressive) tumour.The giant cell tumor of bone etiology unknown has several different sayings.Giant cell tumor of bone Main Tissues composition is the giant cells of similar osteoclast and relatively more modest fusiformis or circular stroma cell.Because giant cells resembles osteoclast very much, British scholar Stewart is referred to as " osteoclastoma ".But majority think that basilar cell (Stroma cell) is main, may be from not breaking up phoirocyte (Undif ferentiated con-nective tissue cell).Also the someone thinks the basilar cell from protoblast (Undifferen tiated mesenchymal cell) in not breaking up, and changes into giant cells.The someone thinks that they belong to the endotheliocyte source again.Somebody general's ownership fibrohistiocytic (Fibroushistiocytic cell) source.The giant cell tumor of bone biological behaviour is complicated, and is changeable.
GCT has obvious local aggressive, and recurrence rate is high, and can shift giant cell tumor of bone.Methods for the treatment of is mainly take operative treatment as main at present, and employing is cut erasion and added inactivation treatment, implant from body or homologous cancellus or bone cement, but easily recurrence, recurrence rate reaches 40～70%.And expanded resection or sections amputation art will have a strong impact on the limbs of patient function widely, and has equally relatively high recurrence rate (about 10%).It is invalid to add chemotherapy, also easy sarcomatous change after the radiotherapy, so the treatment of GCT is always more thorny, the methods for the treatment of that the doctor can select is also few.The research that quickening GCT reaches clinically on the basis is imperative.
At present, the research to giant cell tumor of bone on cell levels mainly is to turn out primary cell by clinical living specimen to be studied again, and the pertinent literature report is also fewer.And for giant cell tumor of bone this rare tumour of malignant giant-cell tumor of bone particularly, clinical living specimen is very un-come-at-able, add primary cell through will be dead after limited the going down to posterity very or lose original feature, this has limited the research to giant cell tumor of bone greatly. and at present, the international culture collection unit that confirms of budapest treaty does not also include the bone giant cell tumor strain.The pertinent literature of setting up the GCT cell strain also only has 1993 from one piece (Establishment of a Cell Line from a Human Giant Cell Tumor of Bone) of Japan, and the cell strain CG-1 that mentions in the document can't obtain now.
Summary of the invention
The strain of a kind of people's bone giant cell tumor and application thereof have been the purpose of this invention is to provide.
People's bone giant cell tumor strain behaviour bone giant cell tumor strain GCT-WF provided by the invention, deposit number is CCTCC NO.C2012101, be preserved in Chinese Typical Representative culture collection center on September 18th, 2012 and (be called for short CCTCC, address: Lopa Nationality an ancient woman's ornament mountain, wuchang, wuhan district Wuhan University).
Described people's bone giant cell tumor strain GCT-WF CCTCC NO.C2012101 can be used as the cell model that the research giant cell tumor of bone occurs, giant cell tumor of bone develops or giant cell tumor of bone shifts.
Described people's bone giant cell tumor strain GCT-WF CCTCC NO.C2012101 can be used for setting up the giant cell tumor of bone animal model.
Described people's bone giant cell tumor strain GCT-WF CCTCC NO.C2012101 can be applicable to screening and/or development treatment giant cell tumor of bone medicine.
People's giant cell tumor of bone GCT-WF provided by the invention can be used for the mechanism in generation, development and the transfer of cell levels research giant cell tumor of bone, set up the giant cell tumor of bone animal model, and with it as target sieving or development cancer therapy drug.
Description of drawings
The cellular form figure that Fig. 1 examines under a microscope for inventor's giant cell tumor of bone GCT-WF cell.
Embodiment
With reference to the accompanying drawings, in conjunction with specific embodiments people's bone giant cell tumor strain GCT-WF CCTCC NO.C2012101 of the present invention is described in detail and describes, understand the present invention with better, but should be understood that following embodiment does not limit the scope of the invention.
Method among the following embodiment if no special instructions, is ordinary method.
1. the acquisition of giant cell tumor of bone sample
Giant cell tumor of bone sample of the present invention comes from a giant cell tumor of bone patient, and this patient is the male sex, 35 years old, carries out right femur upper segment giant cell tumor of bone resection operation.From the excision sample, choose active good tumor tissues several piece as giant cell tumor of bone sample of the present invention.
2. former culture
Adopt the tissue block adherent method to carry out the former culture of bone giant cell tumor, use the DMEM substratum that contains 10% foetal calf serum, 37 ℃, CO
2Concentration is 5%, cultivates under the saturated humidity, until the cell in the culture dish grows to about 80%.
3. the cultivation of going down to posterity
Adopt the cell dissociation method to carry out the cultivation of going down to posterity of bone giant cell tumor: the nutrient solution in the culture dish of former culture to be discarded, add 0.25% pancreatin solution, make abundant infiltration; The visual inspection cellular layer when seeing when space, fine needle hole occurring, discards pancreatin solution, and adds the DMEM substratum that contains 10% foetal calf serum and stop digestion, stable be passaged to for the 5th generation after, obtain the bone giant cell tumor strain.
The bone giant cell tumor strain name that obtains is people's giant cell tumor of bone GCT-WF, and be preserved in Chinese Typical Representative culture collection center on September 18th, 2012 and (be called for short CCTCC, the address: Lopa Nationality an ancient woman's ornament mountain, wuchang, wuhan district Wuhan University), deposit number is CCTCC NO. C2012101.
Examine under a microscope people's giant cell tumor of bone GCT-WF cellular form of acquisition, the result as shown in Figure 1, cell is the shuttle type, is similar to the shape of giant cell tumor of bone mesostroma cell.
The doubling time of people's giant cell tumor of bone GCT-WF is about 48 hours.
In the cell cycle of people's giant cell tumor of bone GCT-WF during each the ratio of shared cycle time be: G
0And G
160%, S 17%, G
2With M 23%, wherein G
0Resting stage, G
1The pre-synthesis phase of for DNA, S is the DNA synthesis phase, G
2Be DNA post-synthesis phase, M is division stage.
People's giant cell tumor of bone GCT-WF can be used for the mechanism of generation, development and the transfer of research giant cell tumor of bone, set up the giant cell tumor of bone animal model, and with it as target sieving or development cancer therapy drug.
More than specific embodiments of the invention are described in detail, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, not breaking away from impartial conversion and the modification of doing under the spirit and scope of the present invention, all should contain within the scope of the invention.
Claims (4)
1. people's bone giant cell tumor strain GCT-WF is characterized in that described cell strain deposit number is CCTCC NO.C2012101.
2. the application of the described people's bone giant cell tumor of claim 1 strain GCT-WF CCTCC NO.C2012101 in setting up the cell model that giant cell tumor of bone occurs, giant cell tumor of bone develops or giant cell tumor of bone shifts.
3. the application of the described people's bone giant cell tumor of claim 1 strain GCT-WF CCTCC NO.C2012101 in setting up the giant cell tumor of bone animal model.
4. the application of the described people's bone giant cell tumor of claim 1 strain GCT-WF CCTCC NO.C2012101 in screening treatment giant cell tumor of bone medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104675859A CN102943065A (en) | 2012-11-19 | 2012-11-19 | Cell strain of giant cell tumor of human bone and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104675859A CN102943065A (en) | 2012-11-19 | 2012-11-19 | Cell strain of giant cell tumor of human bone and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102943065A true CN102943065A (en) | 2013-02-27 |
Family
ID=47726055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012104675859A Pending CN102943065A (en) | 2012-11-19 | 2012-11-19 | Cell strain of giant cell tumor of human bone and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102943065A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109749998A (en) * | 2018-12-26 | 2019-05-14 | 中国人民解放军第二军医大学第二附属医院 | A kind of primary bone giant cell tumor strain GCTB28-luc of people and its construction method and application |
-
2012
- 2012-11-19 CN CN2012104675859A patent/CN102943065A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 卢平等: "骨巨细胞瘤中基质细胞成分的探讨", 《北京医学院学报》 * |
| 谢丹等: "骨巨细胞瘤细胞成分和细胞因子在溶骨中的作用", 《中山医科大学学报》 * |
| 赵宁侠等: "骨巨细胞瘤中成纤维样基质细胞体外钙化能力的研究", 《北京医科大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109749998A (en) * | 2018-12-26 | 2019-05-14 | 中国人民解放军第二军医大学第二附属医院 | A kind of primary bone giant cell tumor strain GCTB28-luc of people and its construction method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102266585B (en) | Biological composite patch for female pelvic floor and manufacturing method thereof | |
| CN109517791B (en) | Preparation of conditioned medium for enhancing autophagy umbilical cord mesenchymal stem cells and application of conditioned medium in angiogenesis | |
| MXPA04004534A (en) | Augmentation of organ function. | |
| CN103966158B (en) | A kind of preparation method and applications of periodontal tissue specific cell epimatrix ECM | |
| Wang et al. | Endoprosthetic reconstruction of the proximal humerus after tumour resection with polypropylene mesh | |
| CN104212762A (en) | Method for culture of urine-derived pluripotent stem cells by virtue of in vitro small molecule induction | |
| Hou et al. | Transplantation of human adipose-derived mesenchymal stem cells on a bladder acellular matrix for bladder regeneration in a canine model | |
| Ji et al. | Construction of tissue‐engineered corpus cavernosum with muscle‐derived stem cells and transplantation in vivo | |
| CN102051344A (en) | Human osteosarcoma cell line and mouse in-vivo transplantation model | |
| CN105395571A (en) | New application of exosome secreted by mesenchymal stem cells in treatment of myocardial infarction | |
| Hennes et al. | Endometrial SUSD2+ mesenchymal stem/stromal cells in tissue engineering: advances in novel cellular constructs for pelvic organ prolapse | |
| Cheng et al. | Biocompatibility of polypropylene mesh scaffold with adipose-derived stem cells | |
| Lee et al. | A novel 3D histotypic cartilage construct engineered by supercritical carbon dioxide decellularized porcine nasal cartilage graft and chondrocytes exhibited chondrogenic capability in vitro | |
| CN106367393A (en) | Mouse prostate cancer circulating tumor cell line and prostate cancer circulating tumor cell isolating and culturing method | |
| CN103609519B (en) | The construction process of BALB/C mice primary cutaneous type animal model | |
| CN101831405B (en) | Lung-targeting metastatic human hepatoma cell strain and establishing method thereof | |
| CN102943065A (en) | Cell strain of giant cell tumor of human bone and application thereof | |
| CN101775369A (en) | High-lung-metastasis human breast cancer cell line | |
| ES2778029T3 (en) | Magnetic extracellular matrix | |
| Su et al. | Decellularized extracellular matrix scaffold seeded with adipose-derived stem cells promotes neurorestoration and functional recovery after spinal cord injury through Wnt/β-catenin signaling pathway regulation | |
| CN103013910A (en) | Human degenerative intervertebral disc cartilage endplate stem cell, preparation method and application thereof | |
| CN105343897B (en) | Rhesus liver cancer model, rhesus liver cancer cell strain and application thereof | |
| CN102552997A (en) | Pericardium with stem cell, preparation method and application thereof | |
| CN101831404B (en) | Lymph gland targeted metastatic human hepatoma cell strain and establishing method thereof | |
| CN107446024B (en) | Polypeptide DIP-13 capable of antagonizing RNA binding activity of DDX3 protein and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130227 |