CN102914611A - Temperature balancer with multiple chromatographic columns integrated - Google Patents
Temperature balancer with multiple chromatographic columns integrated Download PDFInfo
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- CN102914611A CN102914611A CN2012103852201A CN201210385220A CN102914611A CN 102914611 A CN102914611 A CN 102914611A CN 2012103852201 A CN2012103852201 A CN 2012103852201A CN 201210385220 A CN201210385220 A CN 201210385220A CN 102914611 A CN102914611 A CN 102914611A
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Abstract
The invention discloses a temperature balancer with multiple chromatographic columns integrated. An upper metal heat conduction block and a lower metal heat conduction block are respectively provided with a plurality of semi-circular chromatographic column grooves and a plurality of semi-circular chromatographic column grooves, and the semi-circular chromatographic column grooves and the semi-circular chromatographic column grooves are identical in number and correspond in positions. The length of each of the upper metal heat conduction block and the lower metal heat conduction block is equal to that of each chromatographic column in a chromatographic column thermostat of a chromatograph instrument. When the temperature balancer is used with the chromatographic column thermostat of the high-performance liquid chromatograph instrument, the temperature balancer with the chromatographic columns integrated can eliminate limits in the aspects of uniform working temperature, stability and uniformity by using the chromatographic column thermostat of the high-performance liquid chromatograph instrument independently since the temperature balancer has good heat conductivity of metal and tightly contacts with the chromatographic column thermostat of the high-performance liquid chromatograph instrument, and efficiency of multi-dimensional high-performance liquid chromatograph analysis is improved.
Description
Technical field
The present invention relates to the temperature homogenizer that a kind of many root chromatogram columns that use are integrated in High Performance Liquid Chromatography post constant temperature oven.
Background technology
The multidimensional high performance liquid chromatography is to utilize the chromatographic column of two or many different in kinds that the material composition in the complex sample is separated.In the detection of multidimensional high performance liquid chromatography, sample first carries out pre-separation in the first dimension chromatographic column, then will through all or part of compositional selecting of pre-separation change in other one or more dissimilar chromatographic column and further separate.The multidimensional high-efficient liquid phase chromatogram technology has sample is carried out pre-service, and the function of separation and concentration can improve separating power and the selectivity of chromatographic system; Can from the various ingredients of complexity, get rid of interfering material, analyze for required component selectively.
In the analytical test of multidimensional high performance liquid chromatography, the working temperature of chromatographic column correctly is set, and keep the post consistent, that stablize and evenly can improve chromatographic column of many root chromatogram columns working temperature to imitate, improve and respectively measure the degree of separation of material chromatographic peak and the symmetry of peak shape, shorten the retention time of measured matter in chromatographic column, reduce the chromatographic column post and press, reduce the wearing and tearing of high performance liquid chromatograph pump, improve sample analysis result's accuracy and repeatability.
In the analytical test of multidimensional high performance liquid chromatography, people set with the chromatographic column constant temperature oven of high performance liquid chromatograph and the working temperature of stable chromatographic column usually.Yet its principle of heating of chromatographic column constant temperature oven of high performance liquid chromatography is to rely on the bottom electric-heating-wire-heating, and air heat conduction is slow.The chromatographic column constant temperature oven of fan-free, hot-air can't circulative convections, and bottom temp is higher when causing heating, and chromatographic column constant temperature oven head temperature is then lower, causes heating of chromatographic column inhomogeneous.Independent place of each chromatographic column in constant temperature oven also can be caused the inconsistent of these chromatographic column working temperatures.In addition, in the high performance liquid chromatography testing process, mobile phase ceaselessly flows in chromatographic system, and this will make the temperature of chromatographic column mobile phase porch and the temperature in exit produce certain temperature difference.Above-mentioned these cause inconsistent, the unstable and inhomogeneous factor of chromatographic column working temperature can affect accuracy and the repeatability of multidimensional efficient liquid phase chromatographic analysis.
Summary of the invention
The object of the present invention is to provide a kind of in High Performance Liquid Chromatography post constant temperature oven, use rational in infrastructure, can effectively keep the chromatographic column working temperature consistent, stable and evenly, and improve the temperature homogenizer that many root chromatogram columns of efficient liquid phase chromatographic analysis efficient are integrated.
Technical solution of the present invention is:
The temperature homogenizer that a kind of many root chromatogram columns are integrated, it is characterized in that: comprise a metal heat-conducting piece, lower metal heat-conducting piece, upper slice metal heat-conducting piece, lower metal heat-conducting piece passes through hinge links, upper slice metal heat-conducting piece, many first round chromatographic column grooves and many second round chromatographic column grooves are set respectively on the lower metal heat-conducting piece, and first round chromatographic column groove is identical with the quantity of second round chromatographic column groove, the position is corresponding to be arranged, on many, second round chromatographic column groove is at upper metal heat-conducting piece, during lower metal heat-conducting piece obvolvent, consist of the column type space of a plurality of placement chromatographic column cylinders; The length of upper slice metal heat-conducting piece, lower metal heat-conducting piece is identical with the chromatographic column cylinder length in the High Performance Liquid Chromatography post constant temperature oven.
The identical length of upper slice metal heat-conducting piece, lower metal heat-conducting piece, many first round chromatographic column grooves, many second round chromatographic column grooves with.
Set temperature homogenizer upper slice has contact with on upper metal heat-conducting piece, sheet has contact with post under set temperature homogenizer on the lower metal heat-conducting piece, lower have contact with post and upper slice have contact with and be used, obvolvent temperature homogenizer, make a metal heat-conducting piece, lower metal heat-conducting piece consist of respectively the column type space of a plurality of placement chromatographic column cylinders by a plurality of first round chromatographic column grooves, a plurality of second round chromatographic column grooves, and with the whole cylinder close contact of a plurality of chromatographic columns.
Described chromatographic column cylinder does not comprise the column cap part at chromatographic column two ends.
The present invention is rational in infrastructure, and its main structure is that it can be fixed on many root chromatogram columns cylinder in the same metal heat-conducting piece, makes these chromatographic column cylinders and metal heat-conducting agllutination synthetic whole with many metal heat-conducting pieces to semi-circular chromatographic column groove that can obvolvent.The temperature homogenizer that is integrated when many root chromatogram columns and High Performance Liquid Chromatography post constant temperature oven merge when using, because the thermal conductive resin of its metal of temperature homogenizer of being integrated of many root chromatogram columns and with the close contact of High Performance Liquid Chromatography post constant temperature oven, can promote High Performance Liquid Chromatography post constant temperature oven heat transfer efficiency, shorten its thermally equilibrated time, and keep the consistent, stable and even of chromatographic column working temperature.
Description of drawings
The invention will be further described below in conjunction with drawings and Examples.
Fig. 1 is the structural representation of one embodiment of the invention.
Among the figure:
The temperature homogenizer upper slice have contact with: have contact with post with sheet under the temperature homogenizer and be used, obvolvent temperature homogenizer.
2. upper slice metal heat-conducting piece of temperature homogenizer: quick conductive, evenly by the temperature of obvolvent chromatographic column.
3. upper slice semi-circular chromatographic column groove of temperature homogenizer: when the obvolvent of temperature homogenizer, form the column type space of placing the first chromatographic column cylinder with the semi-circular chromatographic column groove 8 of sheet under the temperature homogenizer.
4. upper slice semi-circular chromatographic column groove of temperature homogenizer: when the obvolvent of temperature homogenizer, form the column type space of placing the second chromatographic column cylinder with the semi-circular chromatographic column groove 9 of sheet under the temperature homogenizer.
5. upper slice semi-circular chromatographic column groove of temperature homogenizer: when the obvolvent of temperature homogenizer, form the column type space of placing the 3rd chromatographic column cylinder with the semi-circular chromatographic column groove 10 of sheet under the temperature homogenizer.
The temperature homogenizer upper slice, lower metal heat-conducting block chaining hinge: chain sheet, lower metal heat-conducting piece, and make it free-open-close.
7. sheet metal heat-conducting piece under the temperature homogenizer: quick conductive, evenly by the temperature of obvolvent chromatographic column.
8. lower of the temperature homogenizer semi-circular chromatographic column groove: when the obvolvent of temperature homogenizer, form the column type space of placing the first chromatographic column cylinder with the semi-circular chromatographic column groove 3 of temperature homogenizer upper slice.
9. lower of the temperature homogenizer semi-circular chromatographic column groove: when the obvolvent of temperature homogenizer, form the column type space of placing the second chromatographic column cylinder with the semi-circular chromatographic column groove 4 of temperature homogenizer upper slice.
10. the semi-circular chromatographic column groove of sheet under the temperature homogenizer: when the obvolvent of temperature homogenizer, form the column type space of placing the 3rd chromatographic column cylinder with the semi-circular chromatographic column groove 5 of temperature homogenizer upper slice.
11. lower of the temperature homogenizer is had contact with post: have contact with temperature homogenizer upper slice and be used obvolvent temperature homogenizer.
Embodiment
The temperature homogenizer that a kind of many root chromatogram columns are integrated, comprise a metal heat-conducting piece 2, lower metal heat-conducting piece 7, upper slice metal heat-conducting piece, lower metal heat-conducting piece is by hinge 6 links, upper slice metal heat-conducting piece, many first round chromatographic column grooves 3 are set respectively on the lower metal heat-conducting piece, 4,5 and many second round chromatographic column grooves 8,9,10, and first round chromatographic column groove is identical with the quantity of second round chromatographic column groove, the position is corresponding to be arranged, on many, second round chromatographic column groove is at upper metal heat-conducting piece, during lower metal heat-conducting piece obvolvent, consist of the column type space of a plurality of placement chromatographic column cylinders; The length of upper slice metal heat-conducting piece, lower metal heat-conducting piece is identical with the chromatographic column cylinder length in the high performance chromatograph chromatographic column constant temperature oven.
The identical length of upper slice metal heat-conducting piece, lower metal heat-conducting piece, many first round chromatographic column grooves, many second round chromatographic column grooves with.
Set temperature homogenizer upper slice has contact with 1 on upper metal heat-conducting piece, sheet has contact with post 11 under set temperature homogenizer on the lower metal heat-conducting piece, lower have contact with post and upper slice have contact with and be used, obvolvent temperature homogenizer, make a metal heat-conducting piece, lower metal heat-conducting piece consist of respectively the column type space of a plurality of placement chromatographic column cylinders by a plurality of first round chromatographic column grooves, a plurality of second round chromatographic column grooves, and with the whole cylinder close contact of a plurality of chromatographic columns.
Described chromatographic column cylinder does not comprise the column cap part at chromatographic column two ends.
The course of work of this equipment
(1) connects chromatographic column
Choose used chromatographic column 1, turn on the post lid at chromatographic column two ends, chromatographic column is linked to each other with the mobile phase inlet/outlet pipe respectively according to the direction of arrow, with special spanner outlet and the porch of chromatographic column are tightened.Connect successively chromatographic column 2 and chromatographic column 3 with above-mentioned identical method.
(2) chromatographic column is installed in the chromatogram column temperature homogenizer that many root chromatogram columns are integrated (hereinafter to be referred as the temperature homogenizer)
After temperature homogenizer upper slice had contact with (1) sheet under the temperature homogenizer and have contact with post (11) and remove, upwards open temperature homogenizer upper slice metal heat-conducting piece (2), successively chromatographic column 1 cylinder that connects is put into the semi-circular chromatographic column groove of sheet (8) under the temperature homogenizer, chromatographic column 2 cylinders that connect are put into the semi-circular chromatographic column groove of sheet (9) under the temperature homogenizer, chromatographic column 3 cylinders that connect are put into the semi-circular chromatographic column groove of sheet (10) under the temperature homogenizer.Move down temperature homogenizer upper slice metal heat-conducting piece (2), make sheet metal heat-conducting piece (7) closure under temperature homogenizer upper slice metal heat-conducting piece and the temperature homogenizer.The semi-circular chromatographic column groove of sheet (8) under the semi-circular chromatographic column groove of formation temperature homogenizer upper slice (3) and the temperature homogenizer, the semi-circular chromatographic column groove of sheet (9) under the semi-circular chromatographic column groove of temperature homogenizer upper slice (4) and the temperature homogenizer, the semi-circular chromatographic column groove of sheet (10) is distinguished 3 cylinder type spaces of obvolvent under the semi-circular chromatographic column groove of temperature homogenizer upper slice (5) and the temperature homogenizer, and forms close contact with the whole cylinder of chromatographic column.Mobile temperature homogenizer upper slice has contact with (1), makes it to catch on that sheet has contact with post (11) under the temperature homogenizer, forms the integral body that many root chromatogram columns cylinder and metal heat-conducting piece are combined closely.
(3) the chromatogram column temperature homogenizer that many root chromatogram columns is integrated is put into High Performance Liquid Chromatography post constant temperature oven
After finishing above-mentioned action, the bottom of sheet metal heat-conducting piece (7) under the temperature homogenizer of this many root chromatogram columns of obvolvent cylinder is placed on the bottom of High Performance Liquid Chromatography post constant temperature oven, loam cake and the fastening of the High Performance Liquid Chromatography of closing post constant temperature oven.The top of the temperature homogenizer upper slice metal heat-conducting piece (2) that many root chromatogram columns are integrated is contacted with chromatographic column constant temperature case lid.
(4) start detects
Then open routinely high performance liquid chromatograph (opening successively general supply, voltage stabilizer, pump, detecting device, chromatographic column constant temperature oven, computer monitor and main frame), working temperature and other parameter of chromatographic column is set, behind the preheating certain hour, begin to detect.
Claims (3)
1. the temperature homogenizer that is integrated of root chromatogram column more than a kind, it is characterized in that: comprise a metal heat-conducting piece, lower metal heat-conducting piece, upper slice metal heat-conducting piece, lower metal heat-conducting piece passes through hinge links, upper slice metal heat-conducting piece, many first round chromatographic column grooves and many second round chromatographic column grooves are set respectively on the lower metal heat-conducting piece, and first round chromatographic column groove is identical with the quantity of second round chromatographic column groove, the position is corresponding to be arranged, on many, second round chromatographic column groove is at upper metal heat-conducting piece, during lower metal heat-conducting piece obvolvent, consist of the column type space of a plurality of placement chromatographic column cylinders; The length of upper slice metal heat-conducting piece, lower metal heat-conducting piece is identical with the chromatographic column cylinder length in the chromatograph constant temperature oven.
2. the temperature homogenizer that is integrated of many root chromatogram columns according to claim 1 is characterized in that: the identical length of upper slice metal heat-conducting piece, lower metal heat-conducting piece, many first round chromatographic column grooves, many second round chromatographic column grooves with.
3. the temperature homogenizer that is integrated of many root chromatogram columns according to claim 1 and 2, it is characterized in that: set temperature homogenizer upper slice has contact with on upper metal heat-conducting piece, sheet has contact with post under set temperature homogenizer on the lower metal heat-conducting piece, lower have contact with post and upper slice have contact with and be used, obvolvent temperature homogenizer, make a metal heat-conducting piece, lower metal heat-conducting piece consist of respectively the column type space of a plurality of placement chromatographic column cylinders by a plurality of first round chromatographic column grooves, a plurality of second round chromatographic column grooves, and with the whole cylinder close contact of a plurality of chromatographic columns.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103852201A CN102914611B (en) | 2012-10-12 | 2012-10-12 | Temperature balancer with multiple chromatographic columns integrated |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103852201A CN102914611B (en) | 2012-10-12 | 2012-10-12 | Temperature balancer with multiple chromatographic columns integrated |
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| CN102914611A true CN102914611A (en) | 2013-02-06 |
| CN102914611B CN102914611B (en) | 2013-12-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2012103852201A Expired - Fee Related CN102914611B (en) | 2012-10-12 | 2012-10-12 | Temperature balancer with multiple chromatographic columns integrated |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021120609A1 (en) * | 2020-07-13 | 2021-06-24 | 南通大学 | Chromatography column temperature equalizer for ensuring temperature uniformity |
| WO2021120610A1 (en) * | 2020-07-13 | 2021-06-24 | 南通大学 | Highly-adjustable chromatography column temperature equalizer |
Citations (5)
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| US5983710A (en) * | 1997-11-24 | 1999-11-16 | Brinkman Instruments, Inc. | Modular chromatography column heating system |
| EP1430300A1 (en) * | 2001-09-28 | 2004-06-23 | Systec Inc. | Apparatus for conducting high-temperature liquid chromatography analysis |
| CN1877319A (en) * | 2005-06-06 | 2006-12-13 | 张西咸 | Minisize chromatographic column box with high precision temperature control system |
| CN201666893U (en) * | 2010-04-12 | 2010-12-08 | 青岛盛瀚色谱技术有限公司 | Constant temperature device for ion chromatographic column |
| CN202033335U (en) * | 2011-03-04 | 2011-11-09 | 青岛盛瀚色谱技术有限公司 | Thermostat of ion chromatography conductivity detector |
-
2012
- 2012-10-12 CN CN2012103852201A patent/CN102914611B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5983710A (en) * | 1997-11-24 | 1999-11-16 | Brinkman Instruments, Inc. | Modular chromatography column heating system |
| EP1430300A1 (en) * | 2001-09-28 | 2004-06-23 | Systec Inc. | Apparatus for conducting high-temperature liquid chromatography analysis |
| EP1430300A4 (en) * | 2001-09-28 | 2005-01-26 | Systec Inc | Apparatus for conducting high-temperature liquid chromatography analysis |
| CN1877319A (en) * | 2005-06-06 | 2006-12-13 | 张西咸 | Minisize chromatographic column box with high precision temperature control system |
| CN201666893U (en) * | 2010-04-12 | 2010-12-08 | 青岛盛瀚色谱技术有限公司 | Constant temperature device for ion chromatographic column |
| CN202033335U (en) * | 2011-03-04 | 2011-11-09 | 青岛盛瀚色谱技术有限公司 | Thermostat of ion chromatography conductivity detector |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021120609A1 (en) * | 2020-07-13 | 2021-06-24 | 南通大学 | Chromatography column temperature equalizer for ensuring temperature uniformity |
| WO2021120610A1 (en) * | 2020-07-13 | 2021-06-24 | 南通大学 | Highly-adjustable chromatography column temperature equalizer |
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| CN102914611B (en) | 2013-12-11 |
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