CN102914466B - A kind of method to cell pretreatment to be checked and agents useful for same thereof - Google Patents
A kind of method to cell pretreatment to be checked and agents useful for same thereof Download PDFInfo
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- CN102914466B CN102914466B CN201110217769.5A CN201110217769A CN102914466B CN 102914466 B CN102914466 B CN 102914466B CN 201110217769 A CN201110217769 A CN 201110217769A CN 102914466 B CN102914466 B CN 102914466B
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- body fluid
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- stabilizing agent
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Abstract
The invention provides a kind of method to cell pretreatment to be checked and agents useful for same thereof, the method protects following concrete steps: step 1, be separated: in the container filling the sample gathering or collect, add body fluid solubilising reagent, by the mode of vibrating or stir, sample is fully dissolved; Step 2, protection: the cytoprotection configured and stabilizing agent are joined in above-mentioned body fluid solubilising reagent, by the mode of vibrating or stir, body fluid solubilising reagent and cytoprotection and stabilizing agent are fully merged, complete pre-service; The aqueous solution of this body fluid solubilising reagent to be solution concentration be sodium chloride of 0.5% ~ 25%; This cytoprotection and stabilizing agent are made up of vinegar and edible wine, and the concentration of acid is 0.3 ~ 5%.Method to cell pretreatment to be checked provided by the invention is more effective, safety; pretreated cell to be checked is easier to extract, process and detect, and testing result is more accurate, and; raw material can conveniently obtain, and is very beneficial for environmental protection and the protection to user of service's personal safety.
Description
Technical field
The present invention relates to medical detection method, particularly one carries out pretreated method and agents useful for same thereof to cell to be checked.
Background technology
Medically, in the check processing to cell to be checked such as come off oncocyte, inflammatory cell etc., before detecting cell to be checked, need first to carry out dilution process to sample to be collected and human body fluid (as Pleural effusions, urine etc.), human body containing goo (as phlegm, woman uterus sampling sample, stool etc.).Traditional processing mode is all that the solution such as alcohol, glacial acetic acid, formalin is independent or directly adds in the container of the sample filling collection after mixing; make human body fluid and/or containing goo dissolving and protection carry out simultaneously; easy caking or formation stick together shape; cell to be checked in the sample of collection well can not be separated; thus making the protection of cell inadequate, testing result is not accurate enough.And the cell-protecting used in traditional processing mode is the chemical substances such as alcohol, glacial acetic acid, formalin, specific merchandising location need be arrived and buy, and be unfavorable for environmental protection, also security is lacked to user.
Summary of the invention
The object of this invention is to provide one and pretreated method is carried out to cell to be checked; first pre-service is carried out to the sample gathered; human body fluid and/or human body is made to contain the dissolving of goo and carry out step by step the protection of cell; thus cell to be checked is fully separated, more easily can extract, process and detect cell to be checked.
In order to achieve the above object, the invention provides a kind of method to cell pretreatment to be checked, it is characterized in that, the method protects following concrete steps:
Step 1, is separated: in the container filling the sample gathering or collect, add body fluid solubilising reagent, by the mode of vibrating or stir, sample is fully dissolved, the cell to be checked in body fluid is fully separated;
Step 2, protection: the cytoprotection configured and stabilizing agent are joined in above-mentioned body fluid solubilising reagent, by the mode of vibrating or stir, body fluid solubilising reagent and cytoprotection and stabilizing agent are fully merged, thus reach the protection to cell to be checked, complete pre-service; The sample containing cell to be checked protected is delivered testing agency, enabling it more easily extract when detecting, processing and cell to be checked being detected;
The aqueous solution of described body fluid solubilising reagent to be solution concentration the be sodium chloride of 0.5% ~ 25%, is preferably the aqueous solution of the sodium chloride of 0.5%-5%; This sodium chloride can select edible salt, chemical industry or industrial salt, the preferred salt of this sodium chloride, is more conveniently easy to get.
Described cytoprotection and stabilizing agent are made up of vinegar and edible wine, and the concentration of acid is 0.3 ~ 5%.
The above-mentioned method to cell pretreatment to be checked, wherein, in step 2, described cytoprotection and stabilizing agent proportion in mixed solution is 10-90%.
Present invention also offers the novelty teabag that a kind of aqueous solution by the sodium chloride of described 0.5% ~ 25% is used for body fluid solubilising reagent, adopt the aqueous dissolution body fluid of this sodium chloride or the sample containing goo, fully can dissolve, there will not be large block, sheet, bar or the insoluble shape thing that sticks together that classic method is possible.
Present invention also offers a kind of by the concentration of described acid be 0.3 ~ 5% vinegar and edible wine mixed solution be used as the protection of cell and the novelty teabag of stabilizing agent; adopt this mixed solution as the protection of cell and stabilizing agent; safer, environmental protection, and cheaper starting materials is easy to get.
The present invention passes through to add body fluid solubilising reagent in the container of the sample filling collection; cell to be checked is fully dissociated out; add cytoprotection again and stable reagent adequately protects; more effectively, safety; pretreated cell to be checked is easier to extract, process and detect, and testing result is more accurate.And body fluid solubilising reagent provided by the invention and cytoprotection and stabilizing agent are made up of water, salt, vinegar and edible wine, and these raw materials can obtain easily, and are very beneficial for environmental protection and the protection to user of service's personal safety.
Embodiment
What below introduce is embodiment as content of the present invention, further illustrates described content of the present invention below by embodiment.Certainly, describe the content that following detailed description is only example different aspect of the present invention, and should not be construed as the restriction scope of the invention.
Reference examples
Directly join in the container of the sample filling collection by the mixed liquor of alcohol, glacial acetic acid and formalin or glacial acetic acid and formalin, vibration, makes it fully mix; At this moment we can see that the sample containing goo of collection fully can not dilute dissolving in above-mentioned mixed liquor, the shape thing that sticks together having large block, large tablet, large bar and/or can not dissolve out, we can be wished the cell encapsulation to be checked obtained by these objects, thus affect accuracy of detection.Moreover this liquid is when delivering filtrating test, and large block, sheet, bar in liquid and the shape thing that sticks together can block filtering membrane, and can not reach us fully will process liquid to be checked, accurately detects the object of cell to be checked.Then deliver testing agency, its result as shown in Table 1.
Embodiment
Configuration body fluid solubilising reagent: with water and salt (except salt, chemical industry, industrial sodium chloride also can) preparation body fluid solubilising reagent, make its concentration reach 0.5% ~ 25%.The method determination water of calculating and the ratio of salt can be adopted during obtain solution, thus reach the concentration of needs, also by the concentration of Beaumé scale test solution, salt and/or water can be added respectively, thus reach the concentration of needs.
Prepare cytoprotection and stabilizing agent with vinegar and edible distillate spirit, make the concentration of its acid reach 0.3-5%.The concentration that vinegar marks with purchased product itself is as the criterion; edible wine can buy the product that concentration is 10-90%; calculate their compatibility by the concentration buying vinegar and the edible wine itself come during obtain solution; add vinegar and edible wine respectively; thus reach the concentration of needs, form protective agent.
Step 1, is separated: in the container filling the sample gathering or collect, by the mode of vibrating or stir (preferred mode of oscillation), the cell in body fluid is fully separated; At this moment, we can see that the sample containing goo of collection fully can dilute dissolving, the shape thing that sticks together not having large block, sheet, bar and/or can not dissolve out in above-mentioned mixed liquor, thus can not affect accuracy of detection.This liquid is when delivering filtrating test, and liquid to be checked can adequately protect process by we, reaches the object accurately detecting cell to be checked.
Step 2, protection: the cytoprotection configured and stabilizing agent are added in body fluid solubilising reagent, by the mode of vibrating or stir (preferred mode of oscillation), body fluid solubilising reagent and cytoprotection and stabilizing agent are fully merged, thus reach the protection to cell to be checked; Wherein, cytoprotection and stabilizing agent proportion in mixed solution is 10-90%; Then the sample containing cell to be checked protected is delivered testing agency, enabling it more easily extract when detecting, processing and cell to be checked being detected.
In above-mentioned steps 2, whether add cytoprotection and stabilizing agent depends on the circumstances, if conditions permit, can carrying out to sample detections just need not add cytoprotection and stabilizing agent at once, otherwise then need interpolation.
The sample obtained containing the sample of cell to be checked and the method process of reference examples obtained after adopting the embodiment 1 ~ 5 of said method to process respectively is compared as follows shown in table:
Table one: the result of the method respective pretreatment cell to be checked of embodiment 1 ~ 5 and reference examples
As seen from the above table; by method of the present invention; first cell to be checked is separated; then add cytoprotection and stabilizing agent carries out conservation treatment, after the method process containing in the sample of cell to be checked not containing large block, sheet, bar or the insoluble shape thing that sticks together, can dissolve fully and protect cell to be checked; make its survival rate higher; extract, process, detect more easily, detect degree of accuracy and greatly improve, and avoid blocking the filter membrane checked and filter.
Although give detailed description and explanation to the specific embodiment of the present invention above; but what should indicate is; we can carry out various equivalence according to conception of the present invention to above-mentioned embodiment and change and amendment; its function produced do not exceed that instructions and accompanying drawing contain yet spiritual time, all should within protection scope of the present invention.
Claims (2)
1. to a method for cell pretreatment to be checked in human body fluid, it is characterized in that, the method comprises following concrete steps:
Step 1, is separated: in the container filling the sample gathering or collect, add body fluid solubilising reagent, by the mode of vibrating or stir, sample is fully dissolved, the cell to be checked in body fluid is fully separated;
Step 2, protection: the cytoprotection configured and stabilizing agent are joined in above-mentioned body fluid solubilising reagent, by the mode of vibrating or stir, body fluid solubilising reagent and cytoprotection and stabilizing agent are fully merged, complete pre-service; The aqueous solution of described body fluid solubilising reagent to be solution concentration the be sodium chloride of 0.5% ~ 25%; Described cytoprotection and stabilizing agent are made up of vinegar and edible wine; the concentration of acid is 0.3 ~ 5%; in step 2; described cytoprotection and stabilizing agent proportion in mixed solution is 10-90%; described human body fluid is Pleural effusions or urine, the aqueous solution of described body fluid solubilising reagent to be solution concentration the be sodium chloride of 0.5% ~ 5%.
2. sour concentration be 0.3 ~ 5% vinegar and edible wine mixed solution in the cell pretreatment to be checked of human body fluid, be used as the protection of cell and an application for stabilizing agent, described human body fluid is Pleural effusions or urine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110217769.5A CN102914466B (en) | 2011-08-01 | 2011-08-01 | A kind of method to cell pretreatment to be checked and agents useful for same thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110217769.5A CN102914466B (en) | 2011-08-01 | 2011-08-01 | A kind of method to cell pretreatment to be checked and agents useful for same thereof |
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| Publication Number | Publication Date |
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| CN102914466A CN102914466A (en) | 2013-02-06 |
| CN102914466B true CN102914466B (en) | 2015-08-12 |
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| CN201110217769.5A Expired - Fee Related CN102914466B (en) | 2011-08-01 | 2011-08-01 | A kind of method to cell pretreatment to be checked and agents useful for same thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107024369A (en) * | 2016-02-01 | 2017-08-08 | 马铁军 | To the method for cell pretreatment to be checked in the sticky body fluid of human body |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1828296A (en) * | 2005-12-22 | 2006-09-06 | 云南农业大学 | Maladera sp. spermary chromosome production and observation method |
| CN101283674A (en) * | 2008-06-11 | 2008-10-15 | 南京农业大学 | Breeding process of obtaining radish haploidy |
| CN101775388A (en) * | 2010-01-29 | 2010-07-14 | 大连民族学院 | General and reliable extraction method of total DNA of soil and application thereof |
-
2011
- 2011-08-01 CN CN201110217769.5A patent/CN102914466B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1828296A (en) * | 2005-12-22 | 2006-09-06 | 云南农业大学 | Maladera sp. spermary chromosome production and observation method |
| CN101283674A (en) * | 2008-06-11 | 2008-10-15 | 南京农业大学 | Breeding process of obtaining radish haploidy |
| CN101775388A (en) * | 2010-01-29 | 2010-07-14 | 大连民族学院 | General and reliable extraction method of total DNA of soil and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 不同年龄组女性外周血单核细胞蛋白质组的比较分析;唐利华等;《中国老年学杂志》;20080831;第28卷;第1597页左栏第3段，右栏第2段，摘要 * |
| 张联珠.医学细胞生物学和医学遗传学.《医学细胞生物学和医学遗传学》.2008, * |
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