CN102899209B - Malt and bear manufacturing method for effectively controlling composition of beer higher alcohol and esters - Google Patents

Malt and bear manufacturing method for effectively controlling composition of beer higher alcohol and esters Download PDF

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CN102899209B
CN102899209B CN201210415017.4A CN201210415017A CN102899209B CN 102899209 B CN102899209 B CN 102899209B CN 201210415017 A CN201210415017 A CN 201210415017A CN 102899209 B CN102899209 B CN 102899209B
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wheat
bear
beer
malt
soak
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CN102899209A (en
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方维明
饶胜其
杨振泉
葛庆丰
汪志君
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Guangdong Yongshuntai Baoying Malt Co ltd
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Yangzhou University
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Abstract

The invention discloses a malt and bear manufacturing method for effectively controlling composition of bear high-class alcohol and esters. Barley is used as the main raw material, the barley sprouting is carried out by adding metal ions in certain concentration in a barley dipping section, the finished malt is then used as the main raw material for making bear, the metal ions in certain concentration can be appropriately added in a malt sauce preparation section and a yeast fermentation section, and thus reasonable composition of high-class alcohol and esters can be given to a bear body while the taste of the bear body is improved through the treatment of the metal ions. By utilizing the method disclosed by the invention for making the bear, the malt manufacturing period and the bear fermentation period are obviously shortened, the production cost is greatly lowered, the energy consumption is saved, the quality of the malt, the malt sauce and the finished bear and the taste stability of the bear are all obviously improved, the content and the composition of the high-class alcohol and esters of the finished bear can be effectively controlled in a reasonable range being (4.0 : 1)-(5.5 : 1).

Description

Fructus Hordei Germinatus and method for preparing beer that a kind of effective control beer higher alcohols and ester class form
Technical field
The invention belongs to Fructus Hordei Germinatus and Brewage technical field, Fructus Hordei Germinatus and method for preparing beer that particularly a kind of effective control beer higher alcohols and ester class form.
Background technology
Higher alcohols is the important flavour substances in beer, appropriate existence can make the plentiful mellow and full and fragrance coordination of wine body, but too high levels can be given the rancid sense of people and stimulate taste, and in the situation that drinking amount is few, also can " top " cause headache, wherein n-propyl alcohol, isopropylcarbinol and primary isoamyl alcohol are the important component parts of higher alcohols.Ester class is the main composition composition of beer flavor and the main carriers of beer " fragrance ", aspect style, quality and the formation local flavor typicalness of beer, is playing key effect.Wherein, ethyl acetate, Isoamyl Acetate FCC, isobutyl acetate, Phenylethyl ethanoate and ethyl hexanoate are ester class important in beer.Appropriate ester class can make that wine body is plentiful, smell coordination, but its too high levels is given the uncomfortable bitter taste of beer and fruity, too lowly makes the light widow of beer, is unfavorable for keeping the Harmony of beer flavor.Alcohol ester is than being a very important index weighing sense after beer drink, and suitable alcohol ester specific energy is felt after improving the drink of beer.If alcohol ester is than between 4.0:1~5.5:1, it is soft that the local flavor of beer is coordinated, and also do not have too significantly potato spirit or ester fragrance while judging.Higher alcohols in beer and volatility ester are mainly produced by cereuisiae fermentum metabolism, therefore the height of its content mainly depends on yeast strain and making method.In addition, the quality of wheat juice and each main component allocation proportion also can affect leavening property and the meta-bolites composition of yeast.In recent years, research finds that directly in wheat juice, adding metal ion can produce material impact to brewage; Kind and the content of wheat juice intermediate ion also can affect the fermentative activity of yeast, thereby affect quality and the local flavor of beer.In addition, also studies have reported that, in barley process, adding metal ion has obvious inhibition or activation to the enzyme activity of maltase system, and the activity of enzyme all has great effect to the quality of Fructus Hordei Germinatus and wheat-make cycle.Although some impact that metal ion produces Fructus Hordei Germinatus and brewage has been reported in above-mentioned research, " but the Fructus Hordei Germinatus that metal ion treatment is crossed is for beer brewing; and by metal ion, regulate and control effect and the fermentation stage cereuisiae fermentum physiological metabolism situation of barley germination, malt amylase stage related enzyme systems, with Collaborative Control finished beer higher alcohols and ester class, forms " has no report.
Summary of the invention
The invention provides Fructus Hordei Germinatus and method for preparing beer that a kind of effective control beer higher alcohols and ester class form, the method practicality is wide, can significantly reduce the content of High Alcohols, can obtain thering is reasonable alcohol ester ratio, fragrance is coordinated more, taste more alcohol and beer.
The present invention solves technical problem by the reasonable control of species of metal ion and concentration in wheat, saccharification, fermentation three phases of soaking to beer brewing.
The present invention effectively controls Fructus Hordei Germinatus and the method for preparing beer of beer higher alcohols and ester class composition, comprises the step of soaking wheat, germination baked coke, saccharification and fermentation, wherein:
Soak in wheat step, while soaking wheat the last time, toward soaking Mai Shuizhong, add final concentration 80 ppm NaCl;
In saccharification step, the Fructus Hordei Germinatus crushing and water are put into brew kettle and mix, then add and control MgCl 2final concentration is 60 ppm;
In fermentation step, add and control ZnCl 2final concentration is 0.75 ppm.
In above-mentioned three phases, species of metal ion and concentration are screened by following step:
1. take certain type barley is main raw material, adds respectively the metal ion of final concentration 0,20,40,60,80,100 and 150 ppm soaking the wheat stage with dipping form, and described metal ion can be K +, Na +, Mg 2+, Zn 2+, Ca 2or Cu 2+; In the barley germination stage, investigate the impact of above-mentioned metal ion on Fructus Hordei Germinatus related enzyme systems (comprising amylase, proteolytic enzyme, cellulase, oxydo-reductase etc.) and malt quality;
2. above-mentioned Fructus Hordei Germinatus, after baked coke, utilizes above-mentioned each experimental group metal ion residual content in atomic absorption spectrophotometry monitoring finished product Fructus Hordei Germinatus, and adds as required certain density metal ion; Respectively above-mentioned finished product Fructus Hordei Germinatus is carried out to saccharification to produce wheat juice, and analyze the relevant important indicator of each wheat juice, the impact with the metal ion of investigating each residual concentration on relevant important indicator of malt amylase stage and finished product wheat juice quality;
3. respectively to 2. obtained wheat juice of step, in this stage, investigate step impact that 1. described concentration metal ion forms the physiological property of cereuisiae fermentum, finished beer quality and finished beer Higher Alcohols and ester class;
4. in aforesaid operations implementation process, need concentration of metal ions in sampling timely monitor finished product Fructus Hordei Germinatus, wheat juice and beer; Comparative analysis step 1.~the contained different concns metal ion impact on Fructus Hordei Germinatus, wheat juice and finished beer in 3., with good Fructus Hordei Germinatus, wheat juice and beer quality and reasonably beer higher alcohols and ester consist of guiding, and further optimize under the reference of above-mentioned testing data, determine and to soak best metal ionic species that wheat stage, malt amylase stage and fermentation stage need use and reasonable working concentration thereof, obtaining thering is reasonable alcohol ester, fragrance is coordinated more, taste more alcohol and beer.
The described wheat stage of soaking metal ions addition mode is: while soaking wheat the last time, with dipping form, add; All in the mode of oxymuriate, add; Soaking wheat water is slant acidity pH5.8~6.8.
The metal ions addition mode of described saccharification initial period and fermentation initial period is: according to atomic absorption spectrophotometry, determine before saccharification the metal ion content in wheat juice in Fructus Hordei Germinatus and before fermentation, by adding the form of metal ion mother liquor, metal ion is controlled to finite concentration as required.
Described three phases metal ions addition kind is: can be first add metal ion soaking the wheat stage, after at saccharification initial period and/or fermentation initial period, add metal ion, metal ion (comprises K +, Na +, Mg 2+, Zn 2+and Ca 2+) with independent form, also can add with complex form;
Described metal ion controlled concentration is: soaking the wheat stage is 10~150 ppm, and saccharification initial period is 0~100 ppm, and fermentation initial period is 0~100 ppm.
Its contained limit dextrinase of described raw material barley, α-amylase, beta-amylase and proteinase activity are higher, and ratio is moderate.
Described yeast is brewage yeast, is included as the cereuisiae fermentum of the low yield higher alcohols that testing sieve selects.By the reasonable control to above-mentioned three beer brewings species of metal ion and concentration in the stage, the higher alcohols content in beer is obviously reduced, and alcohol ester specific energy is effectively controlled in 4.0~5.5 scopes; Involving in of metal ion, make Fructus Hordei Germinatus, wheat juice and finished beer quality all be improved significantly, and the flavor stability of beer is also improved significantly.The present invention is easy to operate, is with a wide range of applications.
Embodiment
In order to understand better the present invention, below in conjunction with embodiment, the present invention is further illustrated, but the scope of protection of present invention is not limited to the scope that embodiment represents.
Embodiment:
The Littoral Jiangsu Province brewer's barley Supi No.4 of take carries out test operation as main raw material, and its technical process is: barley is cleaned removal of impurities → soak, and wheat → interpolation metal ion → dry → dig up the roots → finished product Fructus Hordei Germinatus → saccharification → fermentation → storage wine germinates → roast.
Its committed step is as follows:
Soak wheat: at 14 ℃, adopt the cut off the water supply mode of 4 h of immersion 4h to soak wheat, by control, soak the wheat time, wheat degree is soaked in monitoring, make to soak wheat degree and reach 47%, dew point rate is 50%, and toward soaking Mai Shuizhong, adds final concentration 80 ppm NaCl while soaking wheat the last time, soaks to take out barley after wheat and enter the germination stage.
Germinate: 16 ℃ of malting floor temperature controls, malting floor relative humidity, a few days ago more than 85%, is germinateed under uniform temp condition, guarantees that total germinating time reaches 96 h.Dry and adopt temperature programming method, 46 ℃ of 3 h rises to 55 ℃, constant temperature 6 h, and 3 h rise to 64 ℃, constant temperature 4 h, 1 h rises to 77 ℃, constant temperature 1 h, 1 h rises to 84 ℃ and carries out baked coke.
Saccharification: the Fructus Hordei Germinatus that Fructus Hordei Germinatus pulverize → crushes 500 Kg and 46 ~ 47 ℃ of water (material-water ratio is 1:3.8) are put into brew kettle and mixed → add and control MgCl 2final concentration is that wheat juice is filtered in ℃ protein stop ℃ saccharification 50 min → 75,30 min → 65 ℃ in 60 ppm → 50.
Fermentation: wort concentration is adjusted to 12 0p, and be cooled to 8 ℃, adds and controls ZnCl 2final concentration is 0.75 ppm; In above-mentioned wheat juice, inoculate into cereuisiae fermentum, inoculum size is 2.5 * 10 7individual mL -1, in 12 ℃ of main ferment 7 d.
By in aforesaid operations respectively to soaking the Na in wheat stage, malt amylase initial period and beer fermentation initial period +, Mg 2+and Zn 2+regulation and control, make higher alcohols and ester content in finished beer reach respectively 91 mgL -1with 21.5 mgL -1, alcohol ester ratio is 4.2; Compare photograph, higher alcohols content has reduced by 24.3%, and ester content has improved 18.6%, and higher alcohols and ester content are all at zone of reasonableness.

Claims (2)

1. effectively control the method for preparing beer that beer higher alcohols and ester class form, comprise the step of soaking wheat, germination baked coke, saccharification and fermentation, it is characterized in that,
Soak in wheat step, while soaking wheat the last time, toward soaking Mai Shuizhong, add final concentration 80ppm NaCl;
In saccharification step, the Fructus Hordei Germinatus crushing and water are put into brew kettle and mix, then add and control MgCl 2final concentration is 60ppm; In fermentation step, add and control ZnCl 2final concentration is 0.75ppm.
2. the method for preparing beer that effective control beer higher alcohols according to claim 1 and ester class form, is characterized in that, step is as follows:
Soak wheat: at 14 ℃, adopt the cut off the water supply mode of 4h of immersion 4h to soak wheat, by control, soak the wheat time, wheat degree is soaked in monitoring, makes to soak wheat degree and reaches 47%, and dew point rate is 50%, and while soaking wheat the last time, add final concentration 80ppm NaCl toward soaking Mai Shuizhong, soak and take out barley after wheat and enter the germination stage;
Germinate: 16 ℃ of malting floor temperature controls, malting floor relative humidity, a few days ago more than 85%, is germinateed under uniform temp condition, guarantees that total germinating time reaches 96h;
Dry and adopt temperature programming method, 46 ℃ of 3h rise to 55 ℃, constant temperature 6h, and 3h rises to 64 ℃, constant temperature 4h, 1h rises to 77 ℃, constant temperature 1h, 1h rises to 84 ℃ and carries out baked coke;
Saccharification: the Fructus Hordei Germinatus crushing and 46~47 ℃ of water are put into brew kettle by material quality than 1:3.8 and mix, add and control MgCl 2final concentration is 60ppm, 50 ℃ of protein 30min that stops, and 65 ℃ of saccharification 50min, 75 ℃ are filtered wheat juice;
Fermentation: wort concentration is adjusted to 12 ° of P, and is cooled to 8 ℃, add and control ZnCl 2final concentration is 0.75ppm; In above-mentioned wheat juice, inoculate into cereuisiae fermentum, inoculum size is 2.5 * 10 7individual mL -1, in 12 ℃ of main ferment 7d.
CN201210415017.4A 2012-10-26 2012-10-26 Malt and bear manufacturing method for effectively controlling composition of beer higher alcohol and esters Active CN102899209B (en)

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CN106221979B (en) * 2016-08-09 2019-12-10 山东金酿生物科技有限公司 production method of aromatic crystallized malt
CN106554869B (en) * 2016-12-05 2020-07-28 江西宜信堂医疗科技有限公司 Process for preparing beer barley
CN108004071B (en) * 2018-01-18 2020-12-01 江南大学 Method for manufacturing low-turbidity Jiangsu barley malt
CN109181921A (en) * 2018-10-16 2019-01-11 青岛啤酒股份有限公司 Alcohol ester coordination type whole wheat Pilsen malt formula, gained beer and preparation method thereof
CN109181924B (en) * 2018-10-16 2020-12-01 青岛啤酒股份有限公司 Alcohol ester coordinated type wholewheat pearson beer and preparation method thereof
CN113355178B (en) * 2021-06-30 2023-03-28 江苏省农垦麦芽有限公司 Method for reducing crystallization temperature of crystallized malt

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Effective date of registration: 20240519

Address after: No. 188 Baoxiang West Road, Baoying County, Yangzhou City, Jiangsu Province, 225000

Patentee after: Guangdong Yongshuntai (Baoying) Malt Co.,Ltd.

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Address before: 225009 No. 88, South University Road, Jiangsu, Yangzhou

Patentee before: YANGZHOU University

Country or region before: China