CN102898579A - Particle size controllable mono-disperse polyacrylamide gel microspheres and preparation method as well as used device thereof - Google Patents
Particle size controllable mono-disperse polyacrylamide gel microspheres and preparation method as well as used device thereof Download PDFInfo
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Abstract
The invention provides a preparation method of particle size controllable mono-disperse polyacrylamide gel microspheres. The preparation method comprises the following steps of: preparing disperse phase solution and continuous phase solution; controlling flow velocities and viscosities of the disperse phase and the continuous phase; and converging the disperse phase and the continuous phase to obtain polyacrylamide solution droplets with different particle sizes and uniform diameter size, wherein the droplets are subjected to polymerization reaction to obtain the particle size controllable mono-disperse polyacrylamide gel microspheres. The invention also relates to a preparation device of the particle size controllable mono-disperse polyacrylamide gel microspheres and the mono-disperse polyacrylamide gel microspheres obtained thereby. By adjusting the flow velocities and the viscosities of the disperse phase and the continuous phase or the size of capillary tubes, accurate control over the particle size of the microspheres can be realized; and the particle size of the prepared microspheres is within a range of 10-1,000 mum.
Description
Technical field
The present invention relates to the preparation field of mono-dispersion microballoon, relate to specifically controlled single dispersed polyacrylamide gel micro-ball of a kind of particle diameter and preparation method thereof and equipment therefor.
Background technology
Polyacrylamide microsphere has that specific surface area is large, the surface reaction ability strong, be easy to the characteristics such as functionalization and good biocompatibility, has a wide range of applications in a lot of fields.And the single dispersed polyacrylamide microsphere of uniform particle diameter has important use in the high-technology field that analytical chemistry, biological chemistry, immune medical science, standard metering and other have superelevation to require to polyacrylamide particle diameter and size distribution.
Traditional polyacrylamide microsphere preparation method has the preparation methods such as inverse suspension polymerization, inverse emulsion polymerization, dispersion polymerization.The above-mentioned method for preparing polyacrylamide microsphere is difficult to realize the accurate control of microspherulite diameter and the realization of narrow size distribution.
The microfluidic control technology is a brand-new cross discipline that grows up on bases such as microelectronics, micro production, biotechnology and nanotechnologies, and it utilizes the surface tension of liquid and sticking drag force, and (volume is generally 10 to micro liquid or sample
-6~ 10
-15L) handling at micro-scale, process and control, is a kind of new technology of present recent development, and this technology is applied to the preparation of microballoon so that the size distribution control of microballoon and microsphere diameter control have had qualitative leap.
The removable microfluidic control device that makes up with the commercial accessory such as kapillary, plastics tubing, junctor is low with its apparatus cost, be easy to the preparation that the characteristics such as industrialization are used for monodisperse particles has clear superiority.But be subjected to the restriction in capillary size, usually be difficult to prepare particle diameter less than the microballoon of 100 μ m with the microfluidic control device of kapillary base.
Summary of the invention
In order to address the above problem, the purpose of this invention is to provide low, the removable kapillary base flow of a kind of design structure cost body focus type microfluidic control device.
Another object of the present invention provides a kind of method for preparing the controlled single dispersed polyacrylamide gel micro-ball of particle diameter.
An also purpose of the present invention provides the controlled single dispersed polyacrylamide gel micro-ball of particle diameter of aforesaid method preparation.
In order to realize purpose of the present invention, the invention provides the preparation method of the controlled single dispersed polyacrylamide gel micro-ball of a kind of particle diameter, its step is as follows: prepare in proportion disperse phase solution, flow velocity and the viscosity of control disperse phase and external phase, after disperse phase and external phase are converged, obtain the polyacrylamide solution drop of particle diameter difference and diameter dimension homogeneous, described drop gets the controlled single dispersed polyacrylamide gel micro-ball of particle diameter by polyreaction.
More specifically, the method may further comprise the steps:
(1) preparation of disperse phase and external phase: acrylamide monomer, bisacrylamide monomer and initiator are joined form disperse phase solution in the deionized water in proportion, described external phase is low polar compound;
(2) flow velocity and the viscosity of adjustment disperse phase and external phase: control disperse phase flow range is 0.20 ~ 20uLmin
-1, the external phase flow range is 30 ~ 150uLmin
-1, the dispersed phase viscosity scope is 1 ~ 500mPas, external phase range of viscosities 50 ~ 2500mPas;
(3) formation of polyacrylamide drop: after step (2) gained disperse phase solution and continuous phase solution are converged, can obtain the polyacrylamide solution drop of particle diameter difference and diameter dimension homogeneous;
(4) drop forms microballoon: step (3) gained drop generates the controlled single dispersed polyacrylamide gel micro-ball of particle diameter through the thermal-initiated polymerization reaction.
Described preparation method also comprises the aftertreatment of step (5) polypropylene amides gel micro-ball: after the filtration of polypropylene amides gel micro-ball process or decantation (decant) with step (4) gained, organic solvent washing 3 ~ 5 times of residual organic solution, again with after the water-soluble solvent washing 2 ~ 3 times, the elimination solvent, drying namely gets the single dispersed polyacrylamide gel micro-ball.
Described organic solvent refers to nonpolar and weakly polar organic solvent, is preferably hexane, sherwood oil, methylene dichloride, chloroform, or the mixed solvent that forms of the water-soluble solvents such as hexane, sherwood oil, methylene dichloride, chloroform and methyl alcohol, ethanol, acetone; Described water-soluble solvent is methyl alcohol or ethanol.
Wherein, low polar compound is preferably silicone oil, soybean oil, salad oil or kerosene, more preferably silicone oil described in the step (1).
In the step (1), the weight percent of described acrylamide monomer, bisacrylamide monomer, initiator and deionized water is:
Acrylamide monomer: 5% ~ 12%;
Bisacrylamide monomer: 0.05% ~ 0.3% bisacrylamide monomer;
Initiator: 0.1% ~ 0.5%;
Surplus is deionized water.
Described initiator is preferably ammonium persulphate or Potassium Persulphate.Described weight percent is that 0.05% ~ 0.3% bisacrylamide monomer can also be that 0.5 ~ 1.5% vinylformic acid or 0.5 ~ 1.5% MethacryloyloxyethylTrimethyl Trimethyl Ammonium Chloride substitute with weight percent.
In the step (4), the temperature of described polyreaction is preferably 65 ~ 75 ℃, and pressure is normal pressure.
The present invention further provides the used kapillary base flow body focus type microfluidic control device of above-mentioned preparation method, it comprises disperse phase syringe, external phase syringe, disperse phase driving pump, external phase driving pump, T-shaped threeway, polymerization container, described disperse phase syringe and external phase syringe are installed in respectively on disperse phase driving pump and the external phase driving pump, and are connected with two ports of T-shaped threeway by conduit respectively; Wherein, the 3rd port of T-shaped threeway is communicated with conduit, and the liquid in described disperse phase syringe and the external phase syringe flows into polymerization container after compiling in this conduit.
Wherein, be respectively equipped with internal thread in three of described T-shaped threeway ports;
Described external phase syringe is provided with the outside screw that matches well mutually with T-shaped threeway port internal thread with the outside, termination of T-shaped threeway linking conduit;
The conduit that described disperse phase syringe is connected with T-shaped threeway is the 1st kapillary, the interface that described disperse phase syringe is connected with T-shaped threeway is provided with the 1st closed casing, the end outside that the 1st closed casing is connected with T-shaped threeway is provided with the outside screw that matches well mutually with T-shaped threeway port internal thread, 2 bottom surfaces of described the 1st closed casing are the sealing surface with through hole, 2 through holes are on same axis, and the diameter of described through hole and the 1st outside diameter capillaceous are complementary;
The conduit that the 3rd port of described T-shaped threeway is communicated with is the 2nd kapillary, the interface of the 3rd port of described T-shaped threeway is provided with the 2nd closed casing, the end outside that described the 2nd closed casing is connected with T-shaped threeway is provided with the outside screw that matches well mutually with T-shaped threeway port internal thread, the bottom surface of described the 2nd closed casing the other end is the sealing surface with through hole, and the diameter of described through hole and the 2nd outside diameter capillaceous are complementary;
The described the 1st end capillaceous and the 2nd end capillaceous horizontal alignment in the 2nd closed casing, two-port is at a distance of 400 ~ 1200 μ m.Flow out the 1st disperse phase liquid capillaceous and be deformed into the taper fluid at the two-port place by the sticking drag force of the external phase of sudden change of flow speed, form the focus type flow morphology, in the 2nd kapillary, form fine droplet, and along with external phase flows into the downstream.
Preferably, described linking conduit is preferably polyfluortetraethylene pipe;
Preferred, the conduit external diameter that described external phase syringe is connected with T-shaped threeway is 1600um, and internal diameter is 1000um; Described the 1st kapillary external diameter is 160 ~ 750um, and internal diameter is 20 ~ 540um; The described the 2nd external diameter capillaceous is 250 ~ 750 μ m, and internal diameter is 150-540 μ m.
The principle that said apparatus prepares the controlled single dispersion polyethylene alcogel microballoon of particle diameter is as follows: the disperse phase solution and the continuous phase solution that prepare are respectively charged in disperse phase and the external phase syringe, and place respectively on disperse phase and the external phase driving pump, by adjusting fltting speed and the disperse phase size capillaceous of driving pump, control flow velocity, flow and the viscosity of disperse phase and external phase, can obtain the polyvinyl alcohol solution drop of particle diameter difference and diameter dimension homogeneous.
The present invention further provides the controlled single dispersed polyacrylamide gel micro-ball of particle diameter of aforesaid method preparation.
The particle size range of described single dispersed polyacrylamide gel micro-ball is 10 ~ 1000 μ m, and dispersion coefficient CV is less than 5%.
Beneficial effect of the present invention:
The removable kapillary base flow of design structure of the present invention body focus type microfluidic control device prepares the monodispersity polyacrylamide microsphere, comprises negatively charged ion, positively charged ion, non-ionic polyacrylamide microballoon.The particle diameter of the microballoon flow velocity by adjusting disperse phase and external phase and viscosity or capillary size in 10 ~ 1000 mu m ranges can be realized the accurate control of microspherulite diameter.Reduce capillary diameter, increase flow velocity or the viscosity of external phase, reduce the disperse phase flow velocity, the size that can cause the final polyacrylamide microsphere that obtains is corresponding reducing also, otherwise can increase the particle diameter of microballoon.
Description of drawings
The optical microphotograph picture of the non-ionic polyacrylamide microballoon of Fig. 1 embodiment of the invention 2 preparations;
The optical microphotograph picture of the nonionic polyion polyacrylamide microsphere of Fig. 2 embodiment of the invention 3 preparations;
The optical microphotograph picture of the anionic polyacrylamide microballoon of Fig. 3 embodiment of the invention 4 preparations;
The optical microphotograph picture of the cationic polyacrylamide microballoon of Fig. 4 embodiment of the invention 5 preparations;
The optical microphotograph picture of the cationic polyacrylamide microballoon of Fig. 5 embodiment of the invention 6 preparations;
Fig. 6 kapillary base flow of the present invention body focus type microfluidic control device synoptic diagram.
Among the figure, 1-1 external phase driving pump; 1-2 disperse phase driving pump; 2-1 external phase syringe; 2-2 disperse phase syringe; 3-the 1st kapillary; 4-external phase conduit; The threeway of 5-T type; 6-the 2nd kapillary; 7-the 1st closed casing; 8-the 2nd closed casing; The 9-water-bath; The 10-polymerization container.
Embodiment
Below in conjunction with specific embodiment, further set forth associated viscera of the present invention.It is to be noted, these embodiment only are not used in for explanation the present invention and limit the scope of the invention, and, after having read content of the present invention, the relevant technical staff in the field can make various changes or modification to the present invention, and these equivalent form of values fall into the application's appended claims limited range equally.
Embodiment 1
A kind of kapillary base flow body focus type microfluidic control device, it comprises disperse phase syringe 2-2, external phase syringe 2-1, disperse phase driving pump 1-2, external phase driving pump 1-1, T-shaped threeway 5, polymerization container 10, described disperse phase syringe 2-2 and external phase syringe 2-1 are installed in respectively on disperse phase driving pump 1-2 and the external phase driving pump 1-1, and are connected with two ports of T-shaped threeway by conduit 3 and 4 respectively; Wherein, the 3rd port of T-shaped threeway is communicated with conduit 6, flows into polymerization container 10 after the liquid among described disperse phase syringe 2-2 and the external phase syringe 2-1 compiles in this conduit 6.
Wherein, be respectively equipped with internal thread in three of described T-shaped threeway 5 ports;
Described external phase syringe 2-1 is provided with the outside screw that matches well mutually with T-shaped threeway 5 port internal threads with the outside, termination of T-shaped threeway 5 linking conduits 4;
The conduit that described disperse phase syringe 2-2 is connected with T-shaped threeway 5 is the 1st kapillary 3; The interface that described disperse phase syringe 2-2 is connected with T-shaped threeway 5 is provided with the 1st closed casing 7, the end outside that the 1st closed casing 7 is connected with T-shaped threeway 5 is provided with the outside screw that matches well mutually with T-shaped threeway 5 port internal threads, 2 bottom surfaces of described the 1st closed casing 7 are the sealing surface with through hole, 2 through holes are on same axis, and the outside diameter of the diameter of described through hole and the 1st kapillary 3 is complementary; Described the 1st kapillary 3 levels are passed two through holes of the 1st closed casing 7.
The conduit that the 3rd port of described T-shaped threeway 5 is communicated with is the 2nd kapillary 6, the interface of the 3rd port of described T-shaped threeway 5 is provided with the 2nd closed casing 8, the end outside that described the 2nd closed casing 8 is connected with T-shaped threeway 5 is provided with the outside screw that matches well mutually with T-shaped threeway 5 port internal threads, the bottom surface of described the 2nd closed casing 8 the other ends is the sealing surface with through hole, and the outside diameter of the diameter of described through hole and the 2nd kapillary 6 is complementary;
One end of one end of described the 1st kapillary 3 and the 2nd kapillary 6 is at the 2nd closed casing 8 interior horizontal alignments, and two-port is at a distance of 400 ~ 1200 μ m.The disperse phase liquid that flows out the 1st kapillary 3 is deformed into the taper fluid at the two-port place by the sticking drag force of the external phase of sudden change of flow speed, forms the focus type flow morphology, forms fine droplet in the 2nd kapillary, and along with external phase flows into the downstream.
Described linking conduit and kapillary are polyfluortetraethylene pipe;
Preferred, the conduit external diameter that described external phase syringe is connected with T-shaped threeway is 1600um, and internal diameter is 1000um; Described the 1st kapillary external diameter is 245um, and internal diameter is 98um; The described the 2nd external diameter capillaceous is 670 μ m, and internal diameter is 430 μ m.
Embodiment 2
With 10g polyacrylamide, 0.3g N, N'-methylene diacrylamide, 0.2g ammonium persulphate are dissolved in and form disperse phase solution in the 89.5g deionized water.Take dimethyl silicone oil as the external phase material.
Above-mentioned disperse phase solution and continuous phase solution are respectively charged in the syringe of 1mL and 50mL, place on two boost pumps, connect microfluidic controller, it is 2 μ Lmin that the disperse phase flow is set
-1, viscosity 30mPas, external phase flow are 60 μ Lmin
-1, viscosity 600mPas, kapillary 1 is of a size of: external diameter/internal diameter 245/98 μ m, kapillary 2 is of a size of: external diameter/internal diameter 670/430 μ m, open the microfluidic control device, the single reactant solution drop that disperses of preparation; Collect the drop that generates and be incubated 1h in 75 ℃ of water-baths, the reactant drop can polyreaction become polypropylene amides gel micro-ball.Through behind the simple decantation, residual organic solution is with (volume ratio) mixed solution washing in 1: 1 of chloroform and ethanol 4 times, use again washing with alcohol 2 times after, drying namely gets single dispersion non-ionic polyacrylamide microballoon.Fig. 1 is the optical microphotograph picture of thus obtained microsphere, can find out the microspherulite diameter homogeneous, has monodispersity, and particle diameter is about 190 μ m, and CV is 3.27%.
With 10g polyacrylamide, 0.3g N, N'-methylene diacrylamide, 0.2g ammonium persulphate are dissolved in and form disperse phase solution in the 89.5g deionized water.Take dimethyl silicone oil as the external phase material.
Above-mentioned disperse phase solution and continuous phase solution are respectively charged in the syringe of 1mL and 50mL, place on two boost pumps, connect microfluidic controller, it is 4 μ Lmin that the disperse phase flow is set
-1, viscosity 30mPas, external phase flow are 50 μ Lmin
-1, viscosity 600mPas, kapillary 1 is of a size of: external diameter/internal diameter 245/98 μ m, kapillary 2 is of a size of: external diameter/internal diameter 665/531 μ m, open the microfluidic control device, the single reactant solution drop that disperses of preparation; Collect the drop that generates and be incubated 1h in 75 ℃ of water-baths, the reactant drop can polyreaction become polypropylene amides gel micro-ball.Through behind the simple decantation, residual organic solution is with (volume ratio) mixed solution washing in 1: 1 of chloroform and ethanol 4 times, use again washing with alcohol 2 times after, drying namely gets single dispersion non-ionic polyacrylamide microballoon.Fig. 2 is the optical microphotograph picture of thus obtained microsphere, can find out the microspherulite diameter homogeneous, has monodispersity, and particle diameter is about 890 μ m, and CV is 4.13%.
Embodiment 4
With 10g polyacrylamide, 0.3g N, N'-methylene diacrylamide, 0.2g ammonium persulphate are dissolved in and form disperse phase solution in the 89.5g deionized water.Take dimethyl silicone oil as the external phase material.
Above-mentioned disperse phase solution and continuous phase solution are respectively charged in the syringe of 1mL and 50mL, place on two boost pumps, connect microfluidic controller, it is 0.8 μ Lmin that the disperse phase flow is set
-1, viscosity 30mPas, external phase flow are 100 μ Lmin
-1, viscosity 600mPas, kapillary 1 is of a size of: external diameter/internal diameter 245/98 μ m, kapillary 2 is of a size of: external diameter/internal diameter 360/225 μ m, open the microfluidic control device, the single reactant solution drop that disperses of preparation; Collect the drop that generates and be incubated 1h in 75 ℃ of water-baths, the reactant drop can polyreaction become polypropylene amides gel micro-ball.Through behind the simple decantation, residual organic solution is with (volume ratio) mixed solution washing in 1: 1 of chloroform and ethanol 4 times times, use again washing with alcohol 2 times after, drying namely gets single dispersion non-ionic polyacrylamide microballoon.Fig. 3 is the optical microphotograph picture of thus obtained microsphere, can find out the microspherulite diameter homogeneous, has monodispersity, and particle diameter is about 35 μ m, and CV is 4.81%.
9g polyacrylamide, 1g vinylformic acid, 0.2g ammonium persulphate are dissolved in formation disperse phase solution in the 89.8g deionized water.Take dimethyl silicone oil as the external phase material.
Above-mentioned disperse phase solution and continuous phase solution are respectively charged in the syringe of 1mL and 50mL, place on two boost pumps, connect microfluidic controller, it is 2 μ Lmin that the disperse phase flow is set
-1, viscosity 30mPas, external phase flow are 60 μ Lmin
-1, viscosity 600mPas, kapillary 1 is of a size of: external diameter/internal diameter 245/98 μ m, kapillary 2 is of a size of: external diameter/internal diameter 670/430 μ m, open the microfluidic control device, the single reactant solution drop that disperses of preparation; Collect the drop that generates and be incubated 1h in 75 ℃ of water-baths, the reactant drop can polyreaction become polypropylene amides gel micro-ball.Through behind the simple decantation, residual organic solution is with (volume ratio) mixed solution washing in 1: 1 of chloroform and ethanol 4 times, use again washing with alcohol 2 times after, drying namely gets single dispersion anionic polyacrylamide microspheres.Fig. 4 is the optical microphotograph picture of thus obtained microsphere, can find out the microspherulite diameter homogeneous, has monodispersity, and particle diameter is about 180 μ m, and CV is 4.55%.
Embodiment 6
9g polyacrylamide, 1g MethacryloyloxyethylTrimethyl Trimethyl Ammonium Chloride, 0.2g ammonium persulphate are dissolved in formation disperse phase solution in the 89.8g deionized water.Take dimethyl silicone oil as the external phase material.
Above-mentioned disperse phase solution and continuous phase solution are respectively charged in the syringe of 1mL and 50mL, place on two boost pumps, connect microfluidic controller, it is 2 μ Lmin that the disperse phase flow is set
-1, viscosity 30mPas, external phase flow are 60 μ Lmin
-1, viscosity 600mPas, kapillary 1 is of a size of: external diameter/internal diameter 245/98 μ m, kapillary 2 is of a size of: external diameter/internal diameter 670/430 μ m, open the microfluidic control device, the single reactant solution drop that disperses of preparation; Collect the drop that generates and be incubated 1h in 75 ℃ of water-baths, the reactant drop can polyreaction become polypropylene amides gel micro-ball.Through behind the simple decantation, residual organic solution is inferior with (volume ratio) mixed solution washing in 1: 1 of chloroform and ethanol 4 times, use again washing with alcohol 2 times after, drying namely gets the monodisperse cation type polyacrylamide microsphere.Fig. 5 is the optical microphotograph picture of thus obtained microsphere, can find out the microspherulite diameter homogeneous, has monodispersity, and particle diameter is about 190 μ m, and CV is 4.26%.
The above only is preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. the preparation method of the controlled single dispersed polyacrylamide gel micro-ball of a particle diameter, its step is as follows: preparation disperse phase and continuous phase solution, flow velocity and the viscosity of control disperse phase and external phase, after disperse phase and external phase are converged, obtain the polyacrylamide solution drop of particle diameter difference and diameter dimension homogeneous, described drop gets the controlled single dispersed polyacrylamide gel micro-ball of particle diameter by polyreaction.
2. the method for claim 1, it specifically may further comprise the steps:
(1) preparation of disperse phase and external phase: acrylamide monomer, bisacrylamide monomer and initiator are joined form disperse phase solution in the deionized water in proportion, described external phase is low polar compound;
(2) flow velocity and the viscosity of adjustment disperse phase and external phase: control disperse phase flow range is 0.20 ~ 20uLmin
-1, the external phase flow range is 30 ~ 150uLmin
-1, the dispersed phase viscosity scope is 1 ~ 500mPas, external phase range of viscosities 50 ~ 2500mPas;
(3) formation of polyacrylamide drop: after step (2) gained disperse phase solution and continuous phase solution are converged, can obtain the polyacrylamide solution drop of particle diameter difference and diameter dimension homogeneous;
(4) drop forms microballoon: step (3) gained drop generates the controlled single dispersed polyacrylamide gel micro-ball of particle diameter through the thermal-initiated polymerization reaction.
3. method as claimed in claim 2, it is characterized in that, described preparation method also comprises the aftertreatment of step (5) polypropylene amides gel micro-ball: after the filtration of polypropylene amides gel micro-ball process or decantation (decant) with step (4) gained, organic solvent washing 3 ~ 5 times of residual organic solution, again with after the water-soluble solvent washing 2 ~ 3 times, the elimination solvent, drying namely gets the single dispersed polyacrylamide gel micro-ball.
4. method as claimed in claim 2 is characterized in that, low polar compound is silicone oil, soybean oil, salad oil or kerosene described in the step (1).
5. method as claimed in claim 2 is characterized in that, in the step (1), the weight percent of described acrylamide monomer, bisacrylamide monomer, initiator and deionized water is:
Acrylamide monomer: 5% ~ 12%;
Bisacrylamide monomer: 0.05% ~ 0.3%;
Initiator: 0.1% ~ 0.5%;
Surplus is deionized water.
6. method as claimed in claim 2 is characterized in that, in the step (4), the temperature of described polyreaction is 65 ~ 75 ℃, and pressure is normal pressure.
7. the used microfluidic control device of the described method of claim 1 ~ 6 any one, the present invention further provides the used kapillary base flow body focus type microfluidic control device of above-mentioned preparation method, it comprises disperse phase syringe, external phase syringe, disperse phase driving pump, external phase driving pump, T-shaped threeway, polymerization container, described disperse phase syringe and external phase syringe are installed in respectively on disperse phase driving pump and the external phase driving pump, and are connected with two ports of T-shaped threeway by conduit respectively; Wherein, the 3rd port of T-shaped threeway is communicated with conduit, and the liquid in described disperse phase syringe and the external phase syringe flows into polymerization container after compiling in this conduit.
8. device as claimed in claim 7 is characterized in that,
Wherein, be respectively equipped with internal thread in three of described T-shaped threeway ports;
Described external phase syringe is provided with the outside screw that matches well mutually with T-shaped threeway port internal thread with the outside, termination of T-shaped threeway linking conduit;
The conduit that described disperse phase syringe is connected with T-shaped threeway is the 1st kapillary, the interface that described disperse phase syringe is connected with T-shaped threeway is provided with the 1st closed casing, the end outside that the 1st closed casing is connected with T-shaped threeway is provided with the outside screw that matches well mutually with T-shaped threeway port internal thread, 2 bottom surfaces of described the 1st closed casing are the sealing surface with through hole, 2 through holes are on same axis, and the diameter of described through hole and outside diameter capillaceous are complementary;
The conduit that the 3rd port of described T-shaped threeway is communicated with is the 2nd kapillary, the interface of the 3rd port of described T-shaped threeway is provided with the 2nd closed casing, the end outside that described the 2nd closed casing is connected with T-shaped threeway is provided with the outside screw that matches well mutually with T-shaped threeway port internal thread, the bottom surface of described the 2nd closed casing the other end is the sealing surface with through hole, and the diameter of described through hole and the 2nd outside diameter capillaceous are complementary;
The described the 1st end capillaceous and the 2nd end capillaceous horizontal alignment in the 2nd closed casing, two-port is at a distance of 400 ~ 1200 μ m.
9. the controlled single dispersed polyacrylamide gel micro-ball of particle diameter of the described preparation method of claim 1 ~ 7 any one preparation.
10. single dispersed polyacrylamide gel micro-ball as claimed in claim 9 is characterized in that, the particle size range of described polypropylene amides gel micro-ball is 10 ~ 1000 μ m, and dispersion coefficient CV is less than 5%.
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| WO2021147356A1 (en) * | 2020-01-21 | 2021-07-29 | 苏州恒瑞宏远医疗科技有限公司 | Reaction apparatus and processing method thereof, and embolization microsphere preparation device and method for preparation thereof |
| CN115038517A (en) * | 2020-01-21 | 2022-09-09 | 苏州恒瑞宏远医疗科技有限公司 | Reaction device and processing method thereof, and preparation equipment and preparation method of embolism microsphere |
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