CN102895665B - Preparation method of targeting graphene nano-grade drug carrier - Google Patents
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Abstract
The invention relates to a preparation method of a targeting graphene nano-grade drug carrier. The method comprises the steps that: a graphene nano-grade material, a hydrophobic anticancer drug, tumor-targeting molecules, and dopamine are mixed in a water solution; the obtained mixture is subjected to ultrasonic uniform dispersion; a pH value of the mixture is regulated such that the mixture is alkaline; the mixture is stabilized for a certain period of time under magnetic stirring and protection from light; the mixture is subjected to centrifugal separation; and a product is uniformly dispersed in a water solution, such that the targeting graphene nano-grade drug carrier based on the graphene nano-grade material is obtained. The method provided by the invention is characterized in that: dopamine auto-polymerization is induced by the pH value; and the graphene nano-grade material, hydrophobic anticancer drug molecules, and the tumor-targeting molecules are compounded simultaneously during the polymerization process, such that rapid preparation of the targeting drug molecules is realized. The method provided by the invention has the advantages that: the targeting nano-grade drug carrier containing hydrophobic anticancer drug molecules can be rapidly prepared under a water solution environment with a normal temperature; the process of the method is simple, and the operation is convenient; and the method has high practicality and good application prospect.
Description
Technical field
The present invention relates to Novel Carbon Nanomaterials field, be specifically related to a kind of preparation method of targeting graphene nano pharmaceutical carrier.
Background technology
Malignant tumor is a kind of major disease of serious threat human health.The at present clinical comprehensive means such as traditional early diagnosis, radiotherapy, chemotherapy that still adopt are treated.Yet, because it is to sick cell and Normocellular low distinguishing, cause the very big damage to human normal tissue.Therefore the drug-supplying system that, exploitation has a tumor cell specific target tropism seems particularly important in oncotherapy.Kinds of tumor cells special surface label has been found in research at present.The drug-supplying system that is found to be design cancer target of these tumor cell special surface labels provides effective foundation.With respect to traditional radiation and chemotherapy, the high-resolution selectivity of targeting drug delivery system to tumor cell, very little to Normocellular infringement in therapeutic process, thereby its better efficacy.Therefore, the targeted therapy of tumor is the most desirable approach of oncotherapy.
Chemotherapeutics used mainly contains amycin, 5-fluorouracil, paclitaxel etc. at present.These medicines are all hydrophobic drug, conventionally need to carry out chemical modification or modification to increase its water solublity to it, improve its drug utilization degree.And conventional method also needs by the tumor targeted moleculars such as medicament carrier system outside chemical grafting folic acid designed when utilizing these medicines to prepare targeting drug delivery system, this process very complicated, and in chemical graft process, need to use a large amount of organic solvents, cause the raising of manufacturing cost, also environment is brought to bad impact.Along with the proposition of " Green Chemistry " concept, how by the method for easy environmental protection, to prepare targeting tumour medicine drug-supplying system, more and more receive people's concern.
Dopamine is a kind of neurotransmitter existing in blood of human body.Research finds that dopamine auto polymerization can occur and forms poly-dopamine under certain condition, and poly-dopamine has great similarity with naturally occurring mussel Fibronectin on the Nomenclature Composition and Structure of Complexes.Poly-dopamine can, at nearly all material surface Adhesion formation bonding interface, simultaneously due to the activation hydrogen effect on its phenyl ring, make the compound of the reactive groups such as band amino, sulfydryl be easy to be chemically bonded on poly-dopamine surface.
Graphene is a kind of Two-dimensional Carbon nano material of in recent years finding.Due to its unique structure, make it in fields such as biomedicine, composite, sensor, the energy, there is important application prospect.Because Graphene has monoatomic layer structure, its theoretical specific surface area is up to 2600 m
2/ g is applicable to doing pharmaceutical carrier very much.Two basal plane can adsorb medicine simultaneously, so have the unrivaled superelevation carrying drug ratio of other nano materials.And due to its large pi-conjugated structure, can pass through the required hydrophobic drug of π-π interaction fixation for treatment tumor, this just makes it aspect drug delivery system, have a good application prospect.
Summary of the invention
The preparation method that the object of this invention is to provide a kind of targeting graphene nano pharmaceutical carrier, this method overcomes the problems referred to above of conventional method, adopting said method can be prepared targeting tumour medicine drug-supplying system in easy environmental protection ground, has alleviated to greatest extent environmental pollution.
Principle of the present invention, comprises that (1) is that adopted carrier material is graphene nano material, has the features such as specific surface area is large, can pass through π-π interaction association hydrophobic drug, realizes the payload of medicine; (2) be that the formed poly-dopamine layer of biomolecules of dopamine auto polymerization forms shell in graphene nano pharmaceutical carrier outside, there is good biocompatibility, simultaneously for regulating drug rate of release provides a kind of adjustable means; (3) be the characteristic of the spontaneous easy grafting amido polymer in poly-dopamine surface, can convenient environment friendly ground surface grafting folic acid.Utilize dopamine auto polymerization technology, take Graphene as hydrophobic anticancer drug carrier, in conjunction with the characteristic of the spontaneous easy grafting amido polymer in poly-dopamine surface, the antitumor drug drug delivery system of the surperficial folic acid grafting of the quick environmental protection of energy ground preparation.
Preparation method of the present invention is, in concentration is the graphene nano material water solution of 0.1 mg/mL-1 mg/mL, adds hydrophobic anticancer drug molecule, tumor targeted molecular folic acid and dopamine; With the ultrasonic dispersed 10-60 minute of ultrasonic emulsification machine; Regulate pH to 8.0-10.5; Lucifuge magnetic agitation 2-8 hour on magnetic stirrer, centrifuge 3000 rpm-11000 rpm centrifugalize 30-60 minute for the stable complex obtaining, collect nano-particle, use distilled water wash granule at least 3 times; Product is dispersed in solvent, obtains the targeted nano pharmaceutical carrier based on graphene nano material, standby.
In such scheme, described hydrophobic drug is amycin, or 5-fluorouracil.Amycin, 5-fluorouracil are all hydrophobic anticancer drug, Graphene can pass through π-π interact fixing, so in embodiment optionally one test.
In such scheme, the mass ratio of described graphene nano material and hydrophobic anticancer drug molecule is 1:1-5:1.
In such scheme, the mass ratio of described graphene nano material and tumor targeted molecular is 2:1 – 4:1.
In such scheme, the mass ratio of described graphene nano material and dopamine is 1:2-1:8.
In such scheme, the homodisperse solvent of described product is water, or phosphate physiological buffer solution.
The beneficial effect that the present invention obtains is: this nano medicament carrying system only needs by regulating the pH value in aqueous solution, the generation of induction dopamine auto polymerization reaction, just can spontaneous grafting folic acid, and do not need chemical graft folic acid just can realize the preparation of target tumor medicament carrier system.And when realizing the quick preparation of environmental protection, this nano medicament carrying system can be transported to lesions position by hydrophobic anticancer drug orientation, thereby improves the drug effect of lesions position, has the effect of slow release concurrently simultaneously.Targeted nano pharmaceutical carrier is high to the envelop rate of medicine, operating process is easy, and reaction condition is gentle, and can be compared etc. and to be regulated drug loading by reactant quality, that a kind of preparation process is quick and environmental protection, functional and practical novel nano drug-supplying system.
Accompanying drawing explanation
Fig. 1 is the ultraviolet spectrogram of centrifugal rear supernatant in pure amycin solution and targeted drug delivery system preparation process.
Fig. 2 is prepared nano-medicament carrier (DA/GO-(DOX)-FA) the cytotoxicity experiment result to people's choroid melanoma cell (OCM-1), reference group is simple stannic oxide/graphene nano material (GO), amycin medicine (DOX), and the not nano-carrier of carrying medicament (DA/GO-FA) and the not nano-medicament carrier of grafting targeted molecular (DA/GO (DOX)).
The specific embodiment
Embodiment 1
In being the graphene nano material water solution of 0.1 mg/mL, 40mL concentration adds 0.8 mg hydrophobic anticancer drug molecule amycin, 1 mg tumor targeted molecular folic acid and 8 mg dopamine; With ultrasonic emulsification machine ultrasonic dispersed 10 minutes; With pH meter, regulate pH to 8; Lucifuge stirs 2 hours gained stable complexes; With centrifuge, with 3000 rpm rotating speed centrifugalize 60 minutes, collect nano-particle, use distilled water wash granule at least 3 times; Product is dispersed in water, obtains the targeted nano pharmaceutical carrier based on graphene nano material, standby.
Embodiment 2
In being the graphene nano material water solution of 0.5 mg/mL, 40mL concentration adds 10 mg hydrophobic anticancer drug molecule amycin, 6.7 mg tumor targeted molecular folic acid and 100 mg dopamine; With ultrasonic emulsification machine ultrasonic dispersed 40 minutes; With pH meter, regulate pH to 8.5; 5 hours gained stable complexes of lucifuge magnetic agitation on magnetic stirrer; With centrifuge, with 7000 rpm rotating speed centrifugalize 40 minutes, collect nano-particle, use distilled water wash granule at least 3 times; Product is dispersed in phosphate physiological buffer solution, obtains the targeted nano pharmaceutical carrier based on graphene nano material, standby.
Embodiment 3
In being the graphene nano material water solution of 1.0 mg/mL, 40mL concentration adds 40 mg hydrophobic anticancer drug molecule amycin, 20 mg tumor targeted molecular folic acid and 320 mg dopamine; With ultrasonic emulsification machine ultrasonic dispersed 60 minutes; With pH meter, regulate pH to 10.5; 8 hours gained stable complexes of lucifuge magnetic agitation on magnetic stirrer; With centrifuge, with 11000 rpm rotating speed centrifugalize 30 minutes, collect nano-particle, use distilled water wash granule at least 3 times; Product is dispersed in phosphate physiological buffer solution, obtains the targeted nano pharmaceutical carrier based on graphene nano material, standby.
More than the targeted nano medicament carrier system of test preparation, can find out, process is environmental protection, and this has greatly reduced the use of organic reagent.The prepared targeting graphene nano pharmaceutical carrier of above-mentioned 3 tests adopts uv analyzer (Uv) to carry out analyzing and testing, the feature uv absorption wavelength of known amycin is 495nm, as can be seen from Figure 1, in supernatant after centrifugal, contain hardly amycin, the targeted nano pharmaceutical carrier that as can be seen here prepared by the method reaches 100% to the envelop rate of amycin.As can be seen from Figure 2, prepared nano-medicament carrier is the most obvious to the inhibition of tumor cell OCM-1; After grafting tumor targeted molecular FA, the inhibition of cell is further strengthened.Table 1 is the size of nano-medicament carrier prepared under various ratio conditions, carrying drug ratio, and OCM-1 cell is applying the cell survival rate of pharmaceutical carrier after one day.
Table 1
Claims (5)
1. a preparation method for targeting graphene nano pharmaceutical carrier, is characterized in that, adds hydrophobic anticancer drug molecule, tumor targeted molecular and dopamine in graphene nano material water solution; Ultrasonic dispersed, regulator solution pH is to alkalescence; Lucifuge stirs the stable complex obtaining, and after centrifugalize, product is dispersed in solvent, obtains the targeted nano pharmaceutical carrier based on graphene nano material;
Wherein,
The mass ratio of described graphene nano material and hydrophobic anticancer drug molecule is 1:1-5:1;
The mass ratio of described graphene nano material and tumor targeted molecular is 2:1-4:1;
The mass ratio of described graphene nano material and dopamine is 1:2-1:8;
Described hydrophobic anticancer drug molecule is amycin;
Described tumor targeted molecular folic acid.
2. a kind of preparation method of targeting graphene nano pharmaceutical carrier according to claim 1, is characterized in that, the concentration of described graphene nano material water solution is 0.1 mg/mL-1 mg/mL.
3. a kind of preparation method of targeting graphene nano pharmaceutical carrier according to claim 1, is characterized in that, the described dispersed time is 10-60 minute.
4. a kind of preparation method of targeting graphene nano pharmaceutical carrier according to claim 1, is characterized in that, described pH value of solution is 8.0-10.5.
5. a kind of preparation method of targeting graphene nano pharmaceutical carrier according to claim 1, is characterized in that, the described centrifugalize time is 30-60 minute.
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| CN201210290633.1A CN102895665B (en) | 2012-08-15 | 2012-08-15 | Preparation method of targeting graphene nano-grade drug carrier |
| PCT/CN2012/083873 WO2014026435A1 (en) | 2012-08-15 | 2012-10-31 | Preparation method of targeted graphene nano-drug carrier |
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Families Citing this family (9)
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| CN103784407B (en) * | 2014-02-26 | 2016-01-20 | 哈尔滨医科大学 | Graphene oxide-loaded adriamycin nano-particles of a kind of folate-mediated PEG-and preparation method thereof |
| CN104927302A (en) * | 2014-03-20 | 2015-09-23 | 江南大学 | Graphene-toughened epoxy resin composite material and preparation method thereof |
| CN104189917A (en) * | 2014-08-13 | 2014-12-10 | 东华大学 | Preparation method of doxorubicin-containing graphene oxide medicine-carrying composite material |
| CN104399090A (en) * | 2014-11-12 | 2015-03-11 | 深圳先进技术研究院 | Poly dopamine-modified reduced graphene oxide and preparation method and application thereof |
| CN105153381A (en) * | 2015-09-06 | 2015-12-16 | 江南大学 | Novel method for modifying graphene with polymer |
| CN112618514B (en) * | 2020-04-13 | 2022-04-12 | 华东师范大学 | Ammonia borane/hollow mesoporous polydopamine/polyethylene glycol nano composite particle and preparation and application thereof |
| CN111467499A (en) * | 2020-04-18 | 2020-07-31 | 中山市君泽科技有限公司 | Graphene-loaded combined cancer treatment method |
| CN113101370B (en) * | 2021-04-08 | 2022-11-18 | 曲阜师范大学 | Manganese dioxide targeted nano-drug carrier and preparation method and application thereof |
| CN113456672B (en) * | 2021-06-17 | 2023-08-11 | 温州医科大学 | Targeted medicine of chemical kinetics enhancement photothermal therapy system for treating malignant tumor, and preparation method and application thereof |
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