CN102893868B - Method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate plant - Google Patents
Method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate plant Download PDFInfo
- Publication number
- CN102893868B CN102893868B CN201210396990.6A CN201210396990A CN102893868B CN 102893868 B CN102893868 B CN 102893868B CN 201210396990 A CN201210396990 A CN 201210396990A CN 102893868 B CN102893868 B CN 102893868B
- Authority
- CN
- China
- Prior art keywords
- cells
- primordial
- medium
- wedding
- bridal chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 18
- 241000196324 Embryophyta Species 0.000 title abstract description 25
- 230000000408 embryogenic effect Effects 0.000 title abstract 4
- 241001312225 Anthurium scherzerianum Species 0.000 title abstract 2
- 230000008929 regeneration Effects 0.000 claims abstract description 33
- 238000011069 regeneration method Methods 0.000 claims abstract description 33
- 239000002609 medium Substances 0.000 claims description 42
- 210000001161 mammalian embryo Anatomy 0.000 claims description 29
- 239000000725 suspension Substances 0.000 claims description 20
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 17
- 238000009395 breeding Methods 0.000 claims description 11
- 230000001488 breeding effect Effects 0.000 claims description 11
- 239000006160 differential media Substances 0.000 claims description 11
- 238000005286 illumination Methods 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 7
- 230000006698 induction Effects 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- 239000006870 ms-medium Substances 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 206010010254 Concussion Diseases 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 230000009514 concussion Effects 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 3
- 239000012533 medium component Substances 0.000 abstract description 2
- 230000035755 proliferation Effects 0.000 abstract description 2
- 238000004114 suspension culture Methods 0.000 abstract 1
- 230000000392 somatic effect Effects 0.000 description 11
- 230000003442 weekly effect Effects 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241001677259 Acanthophoenix rubra Species 0.000 description 1
- 241001312221 Anthurium Species 0.000 description 1
- 244000209526 Anthurium andraeanum Species 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000006194 liquid suspension Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000005305 organ development Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- OUFRIWNNMFWZTM-UHFFFAOYSA-M sodium arsanilate Chemical compound [Na+].NC1=CC=C([As](O)([O-])=O)C=C1 OUFRIWNNMFWZTM-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate a plant. By changing medium components, light condition and subculture interval of suspension culture in the prior art, an embryogenic cell cluster can proliferate 2.82-3.02 times per week and proliferate 83 times per month, the proliferation efficiency is improved by more than 15 times compared with that in the prior art, the plant regeneration operating process is simplified, and the plant regeneration of the embryogenic cell cluster can be completed by only one step; meanwhile, the plant regeneration time is shortened, and is 6-10 weeks, which is over 20 days shorter than that in the prior art.
Description
Technical field
The present invention relates to plant induction regeneration techniques field, be specifically related to a kind of method of fancy candles lit in the bridal chamber at wedding cells,primordial fast breeding regeneration plant.
Background technology
Fancy candles lit in the bridal chamber at wedding (
anthurium andraeanum) have another name called the red palm, An Zuhua, be Araeceae anthurium, be a kind of important tropical ornamental plants.Be main cut-flower and potted flower source in many tropic countries and regional fancy candles lit in the bridal chamber at wedding, developed into and be only second to the blue second largest tropical flowers commodity in the torrid zone.At present; fancy candles lit in the bridal chamber at wedding seedling is produced the main adventitious organogenesis that adopts; this approach exists the callus induction cycle, and easily there is the shortcomings such as degeneration and variation in length, aseptic seedling; the advantages such as somatic embryo development ways has that body embryo quantity is many, reproduction speed is fast, inheritance stability, the scale seedling that can be fancy candles lit in the bridal chamber at wedding of combining with liquid culture mode is produced effective way is provided.
Fancy candles lit in the bridal chamber at wedding somatic embryo occurs and plant regeneration research starts from the nineties in 20th century.1992, the first passage fancy candles lit in the bridal chamber at wedding blade such as Kuehnle was cultivated, and high-frequency inductor cell stage occurs and obtains complete Regenerated plantlet; 2006, Xin Weijie etc. were induced continuously the embryo callus of shoot proliferation and have been obtained regeneration plant by ' Sonate ' kind blade; 2011, Fitch etc. were so that ' embryo callus that the induction of Marian Seefurth ' kind blade obtains, carries out genetic transformation and obtained transfer-gen plant; 2008, the embryo callus that Xu Chuanying induces taking ' Amigo ' kind blade as material has carried out, cultivated and plant regeneration research by liquid suspension, and the cells,primordial group of acquisition monthly can breed 4 ~ 5 times; Cultivate and bear again complete plantlet through differentiation.
According to open source information, in prior art, fancy candles lit in the bridal chamber at wedding embryonal suspension condition of culture is: 1/2MS+2, and 4-D 1.0mg/L+KT 0 ~ 0.5 mg/L+ sucrose 3%, secretly cultivates, and subculture is spaced apart 25 days.Cells,primordial suspends and cultivates the growth coefficient that is is approximately monthly 4 ~ 5 times.Fancy candles lit in the bridal chamber at wedding embryonal suspension cell group carries out plant regeneration and is divided into four-stage: (1) by embryo callus be inoculated in liquid nutrient medium and cultivate 30 days, form peltate embryo; (2) peltate embryo is inoculated in liquid nutrient medium and is cultivated 20 days, body embryo germination produces white transparence radicle; (3) under liquid condition, carry out illumination cultivation 20 days, radicle turns green and sprouts with plumule; (4) body embryo is transferred and in solid culture medium, carried out Germination And Seedling, after 30 days, sprout true leaf.Whole plant regeneration process need 100 days.In prior art, the growth rate of cells,primordial suspension cultivation system is low, plant regeneration step complexity, and the cycle is longer.
Summary of the invention
The object of the invention is to overcome cells,primordial in prior art and suspend that to cultivate the growth rate of system low, plant regeneration step complexity, the deficiency that the cycle is grown, provides the improvement method of a kind of fancy candles lit in the bridal chamber at wedding cells,primordial fast breeding regeneration plant.
Object of the present invention is achieved by the following technical programs:
A kind of method that fancy candles lit in the bridal chamber at wedding cells,primordial fast breeding regeneration plant are provided, comprises the following steps:
(a) induction forms embryo callus;
(b) set up cells,primordial suspension and cultivate system, obtain the cells,primordial group of 1.0 ~ 2.0 millimeters of diameters;
(c) the cells,primordial group regeneration plant that utilizes step (b) to obtain, the wherein said cells,primordial suspension cultivation system that sets up comprises the steps:
(b1) broken described embryo callus obtains diameter and is no more than the cells,primordial group of 1.0 millimeters;
(b2) the cells,primordial group of 0.1 ~ 0.2 gram is inoculated in suspension medium, 25 ~ 27 DEG C of temperature, illumination 800 ~ 1000 lux, 90 ~ 110 revs/min of concussions are cultivated, wherein said suspension medium is taking MS medium as minimal medium, in every 1 liter of minimal medium, add 2.0 ~ 3.0 milligrams 6-BA, 1.0 ~ 2.0 milligrams 2,4-D, the sucrose of 20 ~ 40 grams, pH value is 5.6 ~ 6.0;
(b3) 6 ~ 8 days subcultures are once, broken by the cells,primordial group of propagation when subculture, by the cells,primordial group of 0.1 ~ 0.2 gram be transferred to fresh suspension medium in, pressing the same terms continues to cultivate, after 3 ~ 4 weeks, cells,primordial is bred in a large number, and acquisition diameter is the cells,primordial group of 1.0 ~ 2.0 millimeters.
In a preferred embodiment, described step (a) comprising:
(a1) get the tender seedling leaf of children or the stem section of fancy candles lit in the bridal chamber at wedding, through surface sterilization processing, blade or stem section are cut into the fragment of 3 ~ 10 millimeters, be inoculated in Induce of embryoid medium;
(a2) in the dark surrounds of 25 ~ 27 DEG C of temperature, cultivate 3 ~ 6 weeks, obtain yellow embryo callus.
In a preferred embodiment, the kind of described fancy candles lit in the bridal chamber at wedding is Alabama.
In a preferred embodiment, described subculture be 7 days once.
In a preferred embodiment, described step (c) comprising:
(c1) cell mass of 1.0 ~ 1.5 millimeters of the diameters obtaining is seeded in differential medium;
(c2) be 1000 ~ 1400 lux in 25 ~ 27 DEG C of temperature, intensity of illumination, cultivate 6 ~ 10 week after in photoenvironment at 10 ~ 14 hours every day, forms green body blast and further grow to form complete plantlet.
In embodiments of the present invention, described differential medium is taking 1/4 ~ 1/20MS medium as minimal medium, every 1 liter of minimal medium adds 6-BA, the sucrose of 10 grams and the agar of 6 ~ 9 grams of 0 ~ 0.01 milligram, pH value is 5.6 ~ 6.0, in a preferred embodiment, described differential medium is taking 1/8 ~ 1/10MS medium as minimal medium, more preferably taking 1/10MS medium as minimal medium.
In a preferred embodiment, in 1 liter of minimal medium, add the 6-BA of 0.005 milligram.
The invention has the beneficial effects as follows: the present invention by change suspend cultivate medium component, illumination condition and subculture interval, cells,primordial group breeds and reaches as high as 3.02 times weekly, monthly can breed 83 times, by the monthly multiple calculating of propagation, improve more than 15 times than the proliferate efficiency of conventional art; And simplified the operating process of plant regeneration, only need a step can complete the plant regeneration of cells,primordial group; Shorten the time of plant regeneration simultaneously, within 6 ~ 10 weeks, completed plant regeneration, saved time more than 20 days than conventional art.
embodiment:
below in conjunction with embodiment, the invention will be further described:
embodiment 1
(1) embryo callus Induce of embryoid: the tender seedling leaf of children of getting fancy candles lit in the bridal chamber at wedding kind Alabama, through surface sterilization processing, blade is cut into the fragment of 5 millimeters, be inoculated in embryo callus Induce of embryoid medium, in the dark surrounds of 26 DEG C of temperature, cultivate 4 weeks, obtain yellow embryo callus;
(2) cells,primordial suspends and cultivates the foundation of system: in the wire netting in 1.0 millimeters, aperture, by embryo callus fragmentation, become diameter to be no more than the cells,primordial group of 1.0 millimeter by wire-mesh screen with tweezers; The cells,primordial group of 0.1 gram is inoculated in 100 milliliters of triangular flasks that are placed with 20 milliliters of cells,primordial suspension mediums, 26 DEG C of temperature, illumination 900 lux, 100 revs/min of concussions are cultivated; When subculture, with said method, the cells,primordial of propagation is rolled into a ball to fragmentation, the cells,primordial group of 0.1 gram is transferred in 100 milliliters of triangular flasks that are placed with 20 milliliters of cells,primordial suspension mediums, continue to cultivate by above-mentioned condition, subculture once weekly.After 3 weeks, cells,primordial is bred in a large number, sets up cells,primordial suspension and cultivates system.
Wherein said cells,primordial suspension medium is taking MS medium as minimal medium, also contain simultaneously 3.0 mg/L 6-BA, 1.0 mg/L 2, the sucrose of 4-D, 30 g/L, pH value is 5.8;
(3) plant regeneration: use the wire netting screening slightly larger in diameter in 1.0 millimeters, aperture in the embryonal suspension cell group of 1.0 millimeters, be transferred in differential medium, be 1200 lux in 26 DEG C of temperature, intensity of illumination, cultivate in photoenvironment at 12 hours every day, after 6 ~ 10 weeks, form green body blast and further grow the complete plantlet of formation.
Wherein said differential medium, for taking 1/10MS medium as minimal medium, also contains 6-BA, the sucrose of 10 g/L and the agar of 8g/L of 0.005 mg/L simultaneously, and pH value is 5.9.
Cultivate by above-mentioned condition, fancy candles lit in the bridal chamber at wedding frequency of embryonic callus induction reaches 96.5%; The cells,primordial of setting up suspends and cultivates is to breed weekly 3.02 times, monthly can breed 83 times; In the cells,primordial group obtaining, somatic embryo formation rate reaches 100%; The shoot regeneration frequency of somatic embryo reaches 55.3 %.
embodiment 2
The present embodiment is got the tender seedling stem of the children section of fancy candles lit in the bridal chamber at wedding kind ' Alabama ' as different from Example 1, through surface sterilization processing, is cut into 10 millimeters of intercepts, is then inoculated in somatic embryo inducement medium and cultivates and obtain somatic embryo, and all the other are identical with embodiment 1.Cultivate by this condition, fancy candles lit in the bridal chamber at wedding frequency of embryonic callus induction reaches 95.1%.
embodiment 3
2 of 6-BA, 2.0 mg/L that contain 2.0 mg/L in cells,primordial suspension medium in step (2) that what the present embodiment was different from embodiment 1 or 2 is, 4-D, all the other are identical with embodiment 1 or 2.Cultivate by this condition, cells,primordial suspends and cultivates is to breed weekly 2.82 times, monthly can breed more than 63 times.
embodiment 4
2 of 6-BA, 2.0 mg/L that contain 3.0 mg/L in cells,primordial suspension medium in step (2) that what the present embodiment was different from embodiment 1 or 2 is, 4-D, all the other are identical with embodiment 1 or 2.Cultivate by this condition, cells,primordial suspends and cultivates is to breed weekly 2.94 times, monthly can breed more than 73 times.
embodiment 5
What the present embodiment was different from embodiment 1 or 2 is that the middle differential medium of step (3) is taking 1/4MS medium as minimal medium; All the other are identical with embodiment 1 or 2.Cultivate by this condition, somatic embryo formation rate reaches 50.62%; The shoot regeneration frequency of somatic embryo reaches 33.92 %.
embodiment 6
What the present embodiment was different from embodiment 1 or 2 is that the middle differential medium of step (3) is taking 1/12 MS medium as minimal medium; All the other are identical with embodiment 1 or 2.Cultivate by this condition, somatic embryo formation rate reaches 91.55%; The shoot regeneration frequency of somatic embryo reaches 41.65%.
Table 1 and table 2 have been summed up the variation that the propagation of cells,primordial in variable concentrations hormone combination situation cultivation stage is bred weekly multiple and monthly bred multiple, and the situation of change of the MS medium diluting using variable concentrations somatic embryo formation rate and shoot regeneration frequency during as minimal medium.
Claims (7)
1. a method for fancy candles lit in the bridal chamber at wedding cells,primordial fast breeding regeneration plant, comprises the steps:
(a) induction forms embryo callus;
(b) set up cells,primordial suspension and cultivate system, obtain the cells,primordial group of 1.0～2.0 millimeters of diameters;
(c) the cells,primordial group regeneration plant that utilizes step (b) to obtain, the wherein said cells,primordial suspension cultivation system that sets up comprises the steps:
(b1) broken described embryo callus obtains diameter and is no more than the cells,primordial group of 1.0 millimeters;
(b2) the cells,primordial group of 0.1～0.2 gram is inoculated in suspension medium, 25～27 DEG C of temperature, illumination 800～1000lux, 90～110 revs/min of concussions are cultivated, wherein said suspension medium is taking MS medium as minimal medium, in every 1 liter of minimal medium, add 2.0～3.0 milligrams 6-BA, 1.0～2.0 milligrams 2,4-D, the sucrose of 20～40 grams, pH value is 5.6～6.0;
(b3) 6～8 days subcultures are once, broken by the cells,primordial group of propagation when subculture, and the cells,primordial group of 0.1～0.2 gram is transferred in fresh suspension medium, continue cultivation by the same terms, after 3～4 weeks, obtain a large amount of cells,primordial group;
Described step (c) comprising:
(c1) cell mass of 1.0～2.0 millimeters of the diameters of acquisition is seeded in differential medium;
(c2) be 1000～1400lux in 25～27 DEG C of temperature, intensity of illumination, cultivate 6～10 week after in photoenvironment at 10～14 hours every day, forms green body blast and further grow to form complete plantlet;
Wherein (c1) described differential medium is taking 1/4～1/20MS medium as minimal medium, and every 1 liter of minimal medium adds 6-BA, the sucrose of 10 grams and the agar of 6～9 grams of 0～0.01 milligram, and pH value is 5.6～6.0.
2. the method for fancy candles lit in the bridal chamber at wedding cells,primordial fast breeding as claimed in claim 1 regeneration plant, described step (a) comprising:
(a1) get the tender seedling leaf of children or the stem section of fancy candles lit in the bridal chamber at wedding, through surface sterilization processing, blade or stem section are cut into the fragment of 3～10 millimeters, be inoculated in Induce of embryoid medium;
(a2) in the dark surrounds of 25～27 DEG C of temperature, cultivate 3～6 weeks, obtain yellow embryo callus.
3. the method for fancy candles lit in the bridal chamber at wedding cells,primordial fast breeding as claimed in claim 2 regeneration plant, wherein the kind of the fancy candles lit in the bridal chamber at wedding of step (a1) is Alabama.
4. the method for fancy candles lit in the bridal chamber at wedding cells,primordial fast breeding as claimed in claim 1 regeneration plant, wherein subculture described in step (b3) be 7 days once.
5. the method for fancy candles lit in the bridal chamber at wedding cells,primordial fast breeding as claimed in claim 1 regeneration plant, wherein said differential medium is taking 1/8～1/10MS medium as minimal medium.
6. the method for fancy candles lit in the bridal chamber at wedding cells,primordial fast breeding as claimed in claim 1 regeneration plant, wherein said differential medium is taking 1/10MS medium as minimal medium.
7. the method for fancy candles lit in the bridal chamber at wedding cells,primordial fast breeding as claimed in claim 1 regeneration plant, wherein every 1 liter of minimal medium adds the 6-BA of 0.005 milligram.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210396990.6A CN102893868B (en) | 2012-10-18 | 2012-10-18 | Method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210396990.6A CN102893868B (en) | 2012-10-18 | 2012-10-18 | Method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102893868A CN102893868A (en) | 2013-01-30 |
| CN102893868B true CN102893868B (en) | 2014-06-18 |
Family
ID=47566592
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210396990.6A Expired - Fee Related CN102893868B (en) | 2012-10-18 | 2012-10-18 | Method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102893868B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107099526A (en) * | 2017-05-09 | 2017-08-29 | 福建省林业科技试验中心 | Red palm somatic hybridization breeding method based on protoplast asymmetric fusion technology |
| CN108739409A (en) * | 2018-08-07 | 2018-11-06 | 苏州大学 | A kind of method that red palm young stem culture is quickly obtained callus |
| CN111053034B (en) * | 2020-01-09 | 2022-08-30 | 上海市农业科学院 | Tissue culture method for generating anthurium embryoid through root tip induction |
| CN114175984A (en) * | 2022-01-18 | 2022-03-15 | 宜兴乾元黑色食品科技有限公司 | Method for inducing leaves of Vaccinium bracteatum plants to rapidly turn red through temperature difference treatment |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0315326A (en) * | 1989-06-12 | 1991-01-23 | Nitto Denko Corp | Production of young seedling of plant of genus pinellia |
| CN1235469C (en) * | 2003-12-19 | 2006-01-11 | 山西省农业科学院园艺研究所 | Quick reproduction of Anzu flower by cell embryo induction |
| CN100424169C (en) * | 2005-01-31 | 2008-10-08 | 肖尊安 | Culture medium for anthurium andraeanum tissue culture and tissue culture seedling breeding method |
| CN100361568C (en) * | 2005-05-12 | 2008-01-16 | 天津龙康泰生物技术有限公司 | Quick breeding method for inducing spheroidal embryo directly from tissue of anthurium |
| WO2007064028A1 (en) * | 2005-12-01 | 2007-06-07 | Kirin Agribio Kabushiki Kaisha | Method for propagation of plant |
-
2012
- 2012-10-18 CN CN201210396990.6A patent/CN102893868B/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| Callus Induction and Plantlet Regeneration in Tissue Cultures of Hawaiian Anthuriums;A.R. Kuehnle et.al.,;《HORTSCIENCE》;19910731;第26卷(第7期);全文 * |
| Improved Transformation of Anthurium;Maureen M.M. Fitch;《HORTSCIENCE》;20110331;第46卷(第3期);全文 * |
| Somatic embryogenesis and plant regeneration in Anthurium andraeanum hybrids;Adelheid R. Kuehnle et.al.,;《Plant Cell Reports》;19921231;第11卷;全文 * |
| Somatic embryogenesis and plant regeneration in Anthurium scherzerianum;Musa Hamidah et.al.,;《Plant Cell,Tissue and Organ Culture》;19971231;第48卷;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102893868A (en) | 2013-01-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101695283B (en) | Culture medium for tissue culture of pedicel buds of oncidium hybridum and tissue cultured seedling propagating method | |
| CN103583358B (en) | A kind of method of dendrobium cultured in vitro regeneration plant | |
| CN102577969B (en) | Breeding method of tissue culture seedling of lonicera macranthoides Yulei No.1 | |
| CN104322375A (en) | Method for rapidly propagating dendrobium chrysotoxumLindl. seeds by tissue culture | |
| CN102845309A (en) | Method for efficiently regenerating plant through Hedychium coccineum Buch.-Ham somatic embryogenesis | |
| CN101953306B (en) | Method for inducing regeneration plant of tetraena mongolica by somatic cell embryo | |
| CN102893868B (en) | Method for embryogenic cells of anthurium scherzerianum to quickly proliferate and regenerate plant | |
| CN102440192B (en) | Culture media for peony high frequency embryogenic callus differentiation, and culture method thereof | |
| CN104642141B (en) | Gerbera adventitious bud induction and plant regeneration method by using root as explant | |
| CN103651137A (en) | Rhynchostylis protocorm rapid breeding method | |
| CN106386486A (en) | Anthurium tissue culture and fast propagation culture medium and anthurium tissue culture and fast propagation seed production method | |
| CN103548676B (en) | Tissue culture rapid propagation method for acer rubrum | |
| CN104686351A (en) | In-vitro rapid propagation method of cercidiphyllum japonicum | |
| CN109819892A (en) | A kind of method for tissue culture of tsaoko fine individual plant | |
| CN103621405B (en) | Artificial corychophramus violaceua seed making method | |
| CN110651713B (en) | Tissue culture method of clematis' Fuji blue | |
| CN105875410B (en) | A kind of rapid propagation method of the hybrid orchid seedling of Chinese cymbidium and Cymbidium hookerianum | |
| CN104082145A (en) | Method for rapidly propagating adiantum soboliferum | |
| CN103548679A (en) | Method for quercus nuttallii somatic embryogenesis | |
| CN104126505A (en) | Somatic embryo in-vitro regeneration method applied to genetic transformation and seed ball rapid propagation of Lilium pumilumDC. Fisch | |
| CN110771512B (en) | Efficient induction method of rabdosia lophanthide callus | |
| CN106613973A (en) | Method for quickly breeding rhododendron molle by approach of regenerating adventitious buds by utilizing tissue culture seedling leaves | |
| CN1491535A (en) | Root inductive method for microbody reproduction of Japan dahurian larch | |
| CN104365477A (en) | Secondary proliferation culture method of color osmanthusfragrans test-tube plantlets | |
| CN104542285A (en) | Method for tissue culture by applying leaves of hemerocallis middendorfii |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yu Bo Inventor after: Liao Feixiong Inventor after: Liu Jinmei Inventor after: Liu Xiaorong Inventor after: Zhu Genfa Inventor after: Jiao Junxi Inventor after: Li Weifeng Inventor before: Yu Bo Inventor before: Liao Feixiong Inventor before: Liu Jinmei Inventor before: Liu Xiaorong Inventor before: Zhu Genfa Inventor before: Jiao Junxi Inventor before: Li Weifeng |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140618 |