CN102876570A - High-flux drug screening microfluidic chip - Google Patents
High-flux drug screening microfluidic chip Download PDFInfo
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- CN102876570A CN102876570A CN2012104210917A CN201210421091A CN102876570A CN 102876570 A CN102876570 A CN 102876570A CN 2012104210917 A CN2012104210917 A CN 2012104210917A CN 201210421091 A CN201210421091 A CN 201210421091A CN 102876570 A CN102876570 A CN 102876570A
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Abstract
The invention provides a high-flux drug screening microfluidic chip which is composed of a cell sample introduction element, a buffer pool, a one-way drive micro-valve, a disk-like bent channel, a drug loading element, a plasmid transfection element, a liquid receiving pool and the like. The elements for cell sample introduction, transfection, cell capture, drug loading and the like are assembled on one chip, so that the invention can simultaneously detect cell integral shape and intracellular labeled protein activity variation under different drug actions on one chip in the detection process, and monitor the action effect of the drug on the actual cells, thereby greatly lowering the detection cost, enhancing the detection accuracy and detection efficiency, and achieving the goal of drug screening.
Description
Technical field
The present invention relates to a kind of high-flux medicaments sifting chip, specifically is a kind of high-flux medicaments sifting micro-fluidic chip.
Technical background
The conventional medicament screening chip mainly by the molecular interaction principle, is fixed in albumen or the nucleic acid molecule of drug targets on the chip, by the reaction process that the record drug molecule is combined with target molecules medicine is screened.The drug reaction of this mode is to carry out under the state that exsomatizes fully, can not represent the caused physiological response of true medicine and change.Micro-fluidic chip has very powerful applying value aspect drug screening, medical diagnosis on disease, environmental monitoring etc. many.Therefore, need at present a kind of novel high-flux medicaments sifting micro-fluidic chip.
Summary of the invention
The object of the present invention is to provide a kind of simple in structure, cost is low and can realize detecting simultaneously cell configuration and the variation of cell inner mark protein-active under the different pharmaceutical effect on the chip piece, the Real Time Monitoring medicine is to the action effect of true cell, improve Detection accuracy and detection efficiency, reach the purpose of drug screening.
In order to address the above problem, technical scheme of the present invention is: a kind of high-flux medicaments sifting micro-fluidic chip is provided.By cell sample introduction element, Buffer Pool, the little valve of unidirectional drive, dish type bending channel, medicine loading element, plasmid transfection element, connect liquid pool etc. and form, it is characterized in that: be attached on the on-chip polydimethylsiloxane section bar, be processed with a cell sample inlet pool, the cell sample inlet pool is communicated with Buffer Pool more than 2; The passage more than 2 is told in the outlet of each Buffer Pool, behind every passage process dish type bending channel, enters in the plasmid transfection element of the cell capture element that comprises each channel bends position embedding; The effect of dish type bending channel is to be that individual cells flows with cell sorting.The plasmid transfection element of the cell capture element of each embedding loads passage with medicine separately and is connected, the sample introduction end of medicine loading passage is equipped with the little valve of unidirectional drive more than 2, ensured in the microchannel, various concentration medicines controllably enter chip in order, react with the detected object on the chip, its reaction process is subject to accurate control, thereby has greatly improved the efficient of reaction, can realize the function that many common biochips can't be finished.The outlet of last plasmid transfection element with connect liquid pool and be connected.
In the technique scheme, described cell capture element is embedded in the channel bends place of each plasmid transfection element, and is interconnected.The specification of cell capture element is different, catches different cells in order to sorting.
As the preferred embodiment of technique scheme, described substrate is transparent material; Connect liquid pool, the cross section is rounded, and the vertical portion is divided into cylindrical, is connected with described plasmid transfection element outlet conduit.
Because each element and device part are interconnected, cooperatively interact, hyperchannel, micro-fluidic detecting pattern so can be provided, when chip being placed on when working on the test set, can realize detecting simultaneously cell configuration and the variation of cell inner mark protein-active under the different pharmaceutical effect on the chip piece, control in real time medicine to the action effect of true cell, reflect the effect that medicine is real-time.
Effect of the present invention is: owing to being assembled with cell sorting element, cell capture element and plasmid transfection element at same chip, in testing process, realization is detecting on the chip piece under the different pharmaceutical effect simultaneously, the variation of cell configuration and cell inner mark protein-active, the monitoring medicine is to the action effect of true cell, greatly reduce testing cost, improve Detection accuracy and detection efficiency, reach the purpose of drug screening.
Description of drawings
Fig. 1 is plan structure schematic diagram of the present invention;
Fig. 2 is dish type bending channel schematic top plan view of the present invention;
Fig. 3 is the enlarged diagram of the cell capture element of plasmid transfection element passage crooked position embedding.
Among the figure: 1. substrate; 2. polydimethylsiloxane section bar; 3. cell sample inlet pool; 4. Buffer Pool; 5. the little valve of unidirectional drive; 6. dish type bending channel; 7. medicine loads passage; 8. plasmid transfection element; 9. the cell capture element 10. connects liquid pool.
Embodiment
Further the present invention is illustrated below in conjunction with drawings and Examples.
Referring to Fig. 1 to Fig. 3: a kind of high-flux medicaments sifting micro-fluidic chip.At first according to concrete drug screening demand, the transfection of specific viable cell biological probe is entered in the target cell, hatch enough time, the cell sample introduction element 3 that the target cell of transfection probe is added chip, target cell enters Buffer Pool 4 subsequently, by dish type bending channel 6 target cell is divided to elect as to enter plasmid transfection element 8 after single target cell is flowed, catch different target cells according to the different size cell capture element 9 that embeds, and make it adhere to growth.Regulate different drug levels by the little valve 5 of the unidirectional drive on the chip, the medicine on chip loads passage 7 and injects the medicine that needs screening, hatches enough time, and medicine and cell are fully reacted; Chip is placed under the fluorescent microscope, gather different wave length fluorescence in the cell with CCD, detect the activity change of specific protein in the cell according to different fluorescent probe efficient, judge that different pharmaceutical is to the practical function effect of cell.Last waste collection is processed to connecing in the liquid pool 10.
Claims (3)
1. high-flux medicaments sifting micro-fluidic chip, by cell sample introduction element (3), Buffer Pool (4), the little valve of unidirectional drive (5), dish type bending channel (6), medicine loading element (7), plasmid transfection element (8), connect liquid pool (10) etc. and form, it is characterized in that: on the polydimethylsiloxane section bar (2) that is attached on the substrate (1), be processed with a cell sample inlet pool (3), cell sample inlet pool (3) is communicated with Buffer Pool (4) more than 2; The passage more than 2 is told in the outlet of each Buffer Pool (4), behind every passage process dish type bending channel (6), enters in the plasmid transfection element (8) of the cell capture element (9) that comprises each channel bends position embedding; The plasmid transfection element (8) of the cell capture element (9) of each embedding loads passage (7) with medicine separately and is connected, and the sample introduction end of medicine loading passage (7) is equipped with the little valve of unidirectional drive (5) more than 2; The outlet of last plasmid transfection element (8) with connect liquid pool (10) and be connected.
2. a kind of high-flux medicaments sifting micro-fluidic chip according to claim 1, it is characterized in that: described cell capture element (9) is embedded in the channel bends place of each plasmid transfection element (8), and is interconnected.
3. a kind of high-flux medicaments sifting micro-fluidic chip according to claim 1, it is characterized in that: described substrate (1) is transparent material; Connect liquid pool (10), the cross section is rounded, and the vertical portion is divided into cylindrical, is connected with the outlet conduit of described plasmid transfection element (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104210917A CN102876570A (en) | 2012-10-29 | 2012-10-29 | High-flux drug screening microfluidic chip |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012104210917A CN102876570A (en) | 2012-10-29 | 2012-10-29 | High-flux drug screening microfluidic chip |
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| CN102876570A true CN102876570A (en) | 2013-01-16 |
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| CN2012104210917A Pending CN102876570A (en) | 2012-10-29 | 2012-10-29 | High-flux drug screening microfluidic chip |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293666A (en) * | 2014-09-11 | 2015-01-21 | 大连理工大学 | Micro-fluidic chip device for detecting interaction between two different unicells |
| CN104498331A (en) * | 2015-01-08 | 2015-04-08 | 青岛大学 | Preparation method and application of single-channel double-concentration-gradient microfluidic chip |
| CN105241940A (en) * | 2015-08-05 | 2016-01-13 | 深圳大学 | Pharmacodynamic detection method and system thereof on the basis of dielectrophoresis force field |
| CN106754241A (en) * | 2016-11-27 | 2017-05-31 | 重庆科技学院 | A kind of application method of the drug screening biochip with air chamber |
| CN106350440B (en) * | 2016-11-27 | 2018-06-26 | 重庆科技学院 | A kind of drug screening biochip with gas chamber |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101550396A (en) * | 2009-05-08 | 2009-10-07 | 深圳先进技术研究院 | High-throughput microfluidic cell chip |
| CN101629143A (en) * | 2008-12-02 | 2010-01-20 | 中国科学院上海微系统与信息技术研究所 | Microfluidic cell array chip for high-throughput medicament screening, method and use |
| CN202912942U (en) * | 2012-10-29 | 2013-05-01 | 重庆科技学院 | Micro-fluidic chip for screening drugs with high throughput |
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2012
- 2012-10-29 CN CN2012104210917A patent/CN102876570A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101629143A (en) * | 2008-12-02 | 2010-01-20 | 中国科学院上海微系统与信息技术研究所 | Microfluidic cell array chip for high-throughput medicament screening, method and use |
| CN101550396A (en) * | 2009-05-08 | 2009-10-07 | 深圳先进技术研究院 | High-throughput microfluidic cell chip |
| CN202912942U (en) * | 2012-10-29 | 2013-05-01 | 重庆科技学院 | Micro-fluidic chip for screening drugs with high throughput |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293666A (en) * | 2014-09-11 | 2015-01-21 | 大连理工大学 | Micro-fluidic chip device for detecting interaction between two different unicells |
| CN104293666B (en) * | 2014-09-11 | 2016-06-22 | 大连理工大学 | The micro flow control chip device of the interphase interaction that two kinds of differences are unicellular |
| CN104498331A (en) * | 2015-01-08 | 2015-04-08 | 青岛大学 | Preparation method and application of single-channel double-concentration-gradient microfluidic chip |
| CN104498331B (en) * | 2015-01-08 | 2015-08-19 | 青岛大学 | The preparation method of the two concentration gradient micro-fluidic chip of a kind of single passage and application thereof |
| CN105241940A (en) * | 2015-08-05 | 2016-01-13 | 深圳大学 | Pharmacodynamic detection method and system thereof on the basis of dielectrophoresis force field |
| CN105241940B (en) * | 2015-08-05 | 2018-04-27 | 深圳大学 | A kind of Composition analyzed method and its system based on the dielectrophoresis field of force |
| CN106754241A (en) * | 2016-11-27 | 2017-05-31 | 重庆科技学院 | A kind of application method of the drug screening biochip with air chamber |
| CN106350440B (en) * | 2016-11-27 | 2018-06-26 | 重庆科技学院 | A kind of drug screening biochip with gas chamber |
| CN106754241B (en) * | 2016-11-27 | 2018-10-23 | 重庆科技学院 | A kind of application method of the drug screening biochip with gas chamber |
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Application publication date: 20130116 |