CN102866190B - Magnesium ion-selective electrode - Google Patents
Magnesium ion-selective electrode Download PDFInfo
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- CN102866190B CN102866190B CN201210108452.2A CN201210108452A CN102866190B CN 102866190 B CN102866190 B CN 102866190B CN 201210108452 A CN201210108452 A CN 201210108452A CN 102866190 B CN102866190 B CN 102866190B
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Abstract
The invention relates to ion detection, in particular to a magnesium ion-selective electrode. The magnesium ion-selective electrode consists of a magnesium ion carrier, an anion site, a polymer material and a plasticizer. The new magnesium ion-selective electrode provided by the invention has higher selectivity and specifity to magnesium ion, and has good measuring precision and a long service life. The capacity of the magnesium ion-selective electrode to inhibit calcium interference is improved by optimizing the proportion of the anion site and the ion carrier. The coefficient of selectivity for calcium is smaller than -0.8, so that the magnesium ion-selective electrode can meet detection and fast response (response time is smaller than 30s) of magnesium ions in serum completely.
Description
Technical field
The present invention relates to ion detection, be specifically related to a kind of magnesium ion electrodes selective.
Background technology
In many Human Physiology chemical processes, magnesium all participates in reacting and accounting for critical role, such as being the activator of plurality of enzymes, is the mankind's the necessary element of inhereditary material nucleic acid, is also the important element that maintains normal neuro-function and muscle function.Whether measure in serum magnesium known body magnesium deficiency.Concentration of serum magnesium reduces can cause that nervimuscular excitability strengthens, and has a convulsion, the symptom such as tetanic, exagger, disorientation.Concentration of serum magnesium raises can there is the symptom that neural muscle excitability is prevented, as felt sick, drowsiness, low blood pressure, breathing are prevented etc.Hypermagnesemia can cause prolonged A-V conduction time.So the detection of the total magnesium of blood plasma and free magnesium is medically having great importance.
Medically, sometimes need the concentration of for example, in accurate detection of biological liquid (serum, blood plasma, urine, spinal fluid or whole blood) magnesium.But, owing to having particularly calcium ion (magnesium and calcium ion have mutual antagonism) of other ions in this class I liquid I, and the quantitative measurment of magnesium is hindered.This interference very obviously (for example, concentration is about 0.6mmol/L) in the time of low magnesium density.In addition, detect in magnesium ion process with full automatic biochemical apparatus, often can be subject to triglyceride, creatine kinase reagent impact (because these two kinds of reagent all part contain magnesium component), testing result often there will be on the low side or higher phenomenon.Therefore, need a kind of facility and the super-sensitive method that is used for measuring the magnesium ion in humoral sample.
In potential measurement humoral sample, magnesium ion concentration can avoid biochemical reagents to detect the interference problem running into.Normally, formed by indicator electrode and contrast electrode for the blomelicalbloodgasandelectrolrteanalyzers of the magnesium ion of potential determination.In the time that indicator electrode and contrast electrode are immersed in same liquid simultaneously, between two electrodes, can produce current potential.This current potential and effects of ion activity are proportional, i.e. Nernst equation.By this equation and the current potential that records, can calculate ion concentration.
Ion-selective electrode is generally made up of ionophore, negative ion site, polymkeric substance and plastifier.These materials are dissolved in solvents tetrahydrofurane, are prepared into uniform solution.After solvents tetrahydrofurane volatilization, get final product to obtain the ion-selective electrode film of transparent and homogeneous.Described ion-selective electrode film intermediate ion carrier can with detect corresponding ion complexation in solution, carry described complex ion to flow through described film, and have precedence over other cation release existing in described sample solution and go out corresponding ion.
Ionophore is the critical material in ion-selective electrode film.The example of magnesium ion carrier comprises: N, N '-diheptyl-N, N'-dimethyl-Isosorbide-5-Nitrae-butane diamides (Helv.Chim.Acta 63,2271,1980);
N, N "-Octamethylenebis (N '-heptyl-N-methyl-methylmalonamide (Mikrochim. Acta III, 283,1988); N, N-Octamethylenebis (N-heptyl-N-methylmalonamide(Anal.Chim.Acta 218,47,1989) etc.Above-mentioned magnesium ion carrier, exists magnesium poor selectivity, affected by calcium larger.Can not meet the detection of serum Mg-ion.
Summary of the invention
In order to solve the problems referred to above of prior art, one of object of the present invention provides a kind of magnesium ion electrodes selective, and it has higher selectivity and selectivity for magnesium ion, good measuring accuracy and very long life-span.Another object of the present invention is to utilize optimization negative ion site and ionophoric ratio to improve magnesium ion electrodes selective to suppress the ability that calcium disturbs.
Magnesium ion electrodes selective of the present invention, is made up of magnesium ion carrier, negative ion site, polymeric material and plastifier.
Preferably,
Described magnesium ion carrier is 1,3,5-tri-[10-(1-adamantyl)-7,9-dioxo-6,10-diaza undecyl] benzene.
Described negative ion site is tetrachlorobenzene boron potassium adjuvant.
Described polymeric material is high molecular weight pvc.
Described plastifier is two glutaric acids two (1-butyl amyl group) decane-1, the potpourri of 10-diester and n-butyl sebacate.
Described magnesium ion electrodes selective, is more preferably made up of the component of following weight percent content:
1,3,5-tri-[10-(1-adamantyl)-7,9-dioxo-6,10-diaza undecyl] benzene 1%
Tetrachlorobenzene boron potassium 3%
Polyvinylchloride 33%
Two glutaric acids two (1-butyl amyl group) decane-1,10-diester 23%
N-butyl sebacate 40%
Magnesium ion of the present invention selects new electrode to have higher selectivity and selectivity for magnesium ion, has good measuring accuracy and very long life-span.And utilize optimization negative ion site and ionophoric ratio to improve magnesium ion electrodes selective and suppress the ability that calcium disturbs.Selectivity factor to calcium is less than-0.8, can meet the detection of serum Mg-ion, response (response time is less than 30s) fast completely.
Magnesium ion electrodes selective of the present invention can be applied in blomelicalbloodgasandelectrolrteanalyzers, and it,, by measuring the current potential of magnesium ion in liquid, calculates magnesium ion concentration according to Nernst equation.
Embodiment
Preferred embodiments more of the present invention are below provided, understand the present invention to help further, but protection scope of the present invention are not limited in these preferred embodiments.
Embodiment 1
Magnesium ion electrodes selective component is as table 1.Take required 1 according to calculating, 3,5-tri-[10-(1-adamantyl)-7,9-dioxo-6,10-diaza undecyl] benzene magnesium ion carrier, tetrachlorobenzene boron potassium, Polyvinylchloride, add therein aequum two glutaric acids two (1-butyl amyl group) decane-1 to contain, 10-diester and n-butyl sebacate mix, fully vibration, and ion electrode material is fully mixed.Then add therein solvents tetrahydrofurane (quality of tetrahydrofuran is the twice of Polyvinylchloride quality), in vortex oscillation device, fully vibrate to mix and prepare magnesium ion film forming liquid.Concrete electrode composition is as following table 1.
The magnesium ion film forming drop preparing is entered in L-type electrode shell, and middle volatilization at normal temperatures 48 hours, makes magnesium ion electrode.The magnesium ion electrodes selective of preparation is applied to blomelicalbloodgasandelectrolrteanalyzers, tests different reagent and can calculate magnesium ion selectivity factor.
Table 1 magnesium ion electrodes selective film component and to calcium selectivity factor
| ? | A | B | C | D | E | F | G |
| Ionophore/mg | 4 | 6 | 10 | 3.5 | 6 | 10 | 30 |
| Tetrachlorobenzene boron potassium mol/mol ionophore | 1.55 | 1.55 | 1.55 | 1.55 | 1.55 | 1.55 | 1.55 |
| Plastifier mixes | 630 | 630 | 620 | 600 | 600 | 590 | 550 |
| PVC | 360 | 360 | 360 | 360 | 360 | 360 | 360 |
| To calcium selectivity factor | -0.8 | -0.9 | -1.0 | -0.9 | -1.05 | -1.2 | -1.4 |
Embodiment 2
Select the magnesium ion electrodes selective that in example 1, G fills a prescription prepared, be applied to the permanent blomelicalbloodgasandelectrolrteanalyzers of uncommon Lay, test senior middle school low value titer, each titer test 10 times, calculates its CV value, and acquired results is as table 2.
Table 2
| ? | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | CV |
| High (1.5mmol/L) | 1.70 | 1.69 | 1.71 | 1.70 | 1.67 | 1.73 | 1.67 | 1.70 | 1.74 | 1.72 | 1.36% |
| Intermediate value (1mmol/L) | `1.22 | 1.25 | 1.25 | 1.19 | 1.20 | 1.23 | 1.25 | 1.21 | 1.18 | 1.18 | 2.50% |
| Low value (0.5mmol/L) | 0.72 | 0.75 | 0.74 | 0.68 | 0.69 | 0.70 | 0.70 | 0.73 | 0.68 | 0.71 | 3.45% |
Embodiment 3
Take each component: 1,3,5-tri-[10-(1-adamantyl)-7,9-dioxo-6,10-diaza undecyl] benzene 1% by following percentage composition
Tetrachlorobenzene boron potassium 3%
Polyvinylchloride 33%
Two glutaric acids two (1-butyl amyl group) decane-1,10-diester 23%
N-butyl sebacate 40%
Mix, fully vibrate, ion electrode material is fully mixed.Then add therein solvents tetrahydrofurane that ion electrode material is dissolved completely, in vortex oscillation device, fully vibrate to mix and prepare magnesium ion film forming liquid.
The magnesium ion film forming drop preparing is entered in L-type electrode shell, and middle volatilization at normal temperatures 48 hours, makes magnesium ion electrode.
Claims (1)
1. a magnesium ion electrodes selective, is characterized in that, is made up of the component of following weight percent content:
1,3,5-tri-[10-(1-adamantyl)-7,9-dioxo-6,10-diaza undecyl] benzene 1%
Tetrachlorobenzene boron potassium 3%
Polyvinylchloride 33%
Two glutaric acids two (1-butyl amyl group) decane-1,10-diester 23%
N-butyl sebacate 40%.
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| CN201210108452.2A CN102866190B (en) | 2012-04-14 | 2012-04-14 | Magnesium ion-selective electrode |
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| CN201210108452.2A CN102866190B (en) | 2012-04-14 | 2012-04-14 | Magnesium ion-selective electrode |
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| CN107209142B (en) * | 2015-02-03 | 2022-09-13 | 西门子医疗保健诊断公司 | Improved magnesium ion selective membranes |
| CN109813788B (en) * | 2017-11-20 | 2024-02-06 | 上海柏中观澈智能科技有限公司 | Ion selective electrode and measurer for detecting ion concentration in liquid substance, and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5911862A (en) * | 1993-09-15 | 1999-06-15 | Chiron Diagnostics Corporation | Material for establishing solid state contact for ion selective electrodes |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5911862A (en) * | 1993-09-15 | 1999-06-15 | Chiron Diagnostics Corporation | Material for establishing solid state contact for ion selective electrodes |
Non-Patent Citations (6)
| Title |
|---|
| Magnesium–tetrazaporphyrin incorporated PVC matrix as a new material for fabrication of Mg2+ selective potentiometric sensor;V.K. Gupta等;《Talanta》;20040311;第63卷(第4期);第1027–1033页 * |
| V.K. Gupta等.Magnesium–tetrazaporphyrin incorporated PVC matrix as a new material for fabrication of Mg2+ selective potentiometric sensor.《Talanta》.2004,第63卷(第4期),第1027–1033页. * |
| 一种基于离子交换原理的PVC 敏感膜的离子识别性能;陈翔等;《解放军预防医学杂志》;20091031;第27卷(第5期);第320页 * |
| 新型三脚架配体1, 3, 5-三-( n-2-5-二氮杂己烷基)-苯的合成及其Co(II) 、Ni(II) 、Cu(II) 、Zn(II) 配合物稳定性研究;盖庆春等;《南开大学学报(自然科学)》;20010630;第34卷(第2期);第13页 * |
| 盖庆春等.新型三脚架配体1, 3, 5-三-( n-2-5-二氮杂己烷基)-苯的合成及其Co(II) 、Ni(II) 、Cu(II) 、Zn(II) 配合物稳定性研究.《南开大学学报(自然科学)》.2001,第34卷(第2期),第13页. * |
| 陈翔等.一种基于离子交换原理的PVC 敏感膜的离子识别性能.《解放军预防医学杂志》.2009,第27卷(第5期),第320页. * |
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