CN102864184A - Method for producing citric acid by utilizing immobilized aspergillus niger - Google Patents
Method for producing citric acid by utilizing immobilized aspergillus niger Download PDFInfo
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- CN102864184A CN102864184A CN2012103119140A CN201210311914A CN102864184A CN 102864184 A CN102864184 A CN 102864184A CN 2012103119140 A CN2012103119140 A CN 2012103119140A CN 201210311914 A CN201210311914 A CN 201210311914A CN 102864184 A CN102864184 A CN 102864184A
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- citric acid
- aspergillus niger
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Abstract
The invention provides a method for producing citric acid by utilizing immobilized aspergillus niger. According to the method, the immobilized aspergillus niger cells are embedded by taking calcium alginate as a carrier to be used for producing the citric acid; and when the citric acid is produced by utilizing the immobilized cells, the optimal sucrose concentration is 120 g/L, the optimal temperature is 30 DEG C, the optimal inoculation dose is 10 percent, the optimal rotation speed is 200 revolutions/minutes, and the influence on the production of the citric acid by utilizing the immobilized cells is more obvious than influence on the production of the citric acid by utilizing free cells.
Description
Technical field
The invention belongs to fermentation technical field, be specifically related to a kind of method of utilizing Citric Acid Production by Immobilized Aspergillus niger.
Background technology
Citric acid is a kind of important organic acid, has another name called Citric Acid, and clear crystal often contains a part crystal water, and odorless has very strong tart flavour, and is soluble in water.Than soluble in the hot water, this character is commonly used to identify and separation of citric acid its calcium salt in cold water.The suitable temperature of control can obtain Citric Acid, usp, Anhydrous Powder during crystallization.In industry, grocery trade, the industry of making up waits has extremely many purposes.At room temperature, citric acid is colourless translucent crystal or white particle or white crystalline powder, the extremely acid of odorless, flavor, and little in malaria have a deliquescence.It can exist with the form without hydrate or monohydrate: citric acid during crystallization, generates without hydrate from hot water; Crystallization then generates monohydrate in cold water.Monohydrate can decompose and obtains without hydrate when being heated to 78 ℃.In the time of 15 degrees centigrade, citric acid also can dissolve in dehydrated alcohol.
Immobilized cell technology is an emerging technology in the bioengineering field, has been applied to all respects such as food and fermentation industries, medicine industry, chemical analysis, environmental purification, energy development and theoretical investigation.In the fermentation field, immobilization technology has been used for organic acid, amino acid, microbiotic, zymin, ethanol and other organic production.Domestic also have the scholar to point out, the trend of citric acid fermentation is to utilize immobilized cell to carry out serialization production.
Summary of the invention
The invention provides a kind of method of utilizing Citric Acid Production by Immobilized Aspergillus niger.
1. method of utilizing Citric Acid Production by Immobilized Aspergillus niger, the present invention is take alginate calcium as carrier embedded immobilization aspergillus niger cell, for the production of citric acid.
2. the substratum of step 1 is NH
4Cl 4 g/L MgSO
47H
2O 0.25 g/L, FeSO
40.25 mg/L CuSO
40.1 mg/L, ZnSO
40.1 mg/L, MnSO
40.2 mg/L.
3. the suitableeest sucrose concentration of step 1 is 120 g/L.
4. the optimum temperuture of step 1 is 30 ℃.
5. the suitableeest inoculum size 10% of step 1.
6. the suitableeest rotating speed of step 1 is 200 r/min.
Beneficial effect of the present invention is: the present invention utilizes calcium alginate gel embedded immobilization aspergillus niger to produce citric acid, further improved the throughput of citric acid, the present invention is take alginate calcium as carrier embedded immobilization aspergillus niger cell, for the production of citric acid, when utilizing Citric Acid Production by Immobilized, the suitableeest sucrose concentration is 120 g/L, optimum temperuture is 30 ℃, the suitableeest inoculum size 10%, the suitableeest rotating speed are that 200 r/min immobilized cells production lemon Gao effect of acid utilizes free cell more obvious.
Description of drawings
The different sucrose additions of Fig. 1 are on the impact of citric acid output.
Fig. 2 different fermentations temperature is on the impact of citric acid output.
Fig. 3 different vaccination amount is on the impact of citric acid output.
Fig. 4 different rotating speeds is on the impact of citric acid output.
Embodiment
The following examples elaborate to the present invention, but to the present invention without limits.
The used bacterial strain of the present invention is aspergillus niger CICC 40332, is preserved in Chinese industrial microbial strains preservation administrative center.
Embodiment 1
The implementation case illustrates different sucrose additions to the impact of citric acid output, for inquiring into different sucrose additions to the impact of citric acid output, uses different sucrose additions to carry out citric acid fermentation, and the result as shown in Figure 1.
As shown in Figure 1, be between the 80-120 g/L time when adding sucrose in the substratum, the output of citric acid increases to some extent, citric acid output is for the highest when the mass concentration of adding sucrose in the substratum is 120 g/L, and when the mass concentration of adding sucrose in the substratum surpasses 120 g/L, the output of citric acid begins to descend, and therefore selecting the addition of sucrose is 120 g/L.
Embodiment 2
The implementation case explanation different fermentations temperature for inquiring into the different fermentations temperature to the impact of citric acid output, uses the different fermentations temperature to carry out citric acid fermentation to the impact of citric acid output, and the result as shown in Figure 2.
As shown in Figure 2, when leavening temperature is between the 400-600 mg/L time, the output of citric acid increases to some extent, citric acid yield increased group improves about 10% for the highest when leavening temperature is 600 mg/L, and when leavening temperature surpasses 600 mg/L, the output of citric acid begins to descend, and therefore selecting leavening temperature is 600 mg/L.
Embodiment 3
The implementation case explanation different vaccination amount for inquiring into the different vaccination amount to the impact of citric acid output, uses the different vaccination amount to carry out citric acid fermentation to the impact of citric acid output, and the result as shown in Figure 3.
As shown in Figure 3, when inoculum size is between the 5%-10% time, the output of citric acid increases to some extent, and when inoculum size is in 10%, citric acid output is for the highest, and when inoculum size surpassed 10%, the output of citric acid did not increase, and therefore selecting inoculum size is 10%.
Embodiment 4
The implementation case explanation different rotating speeds for inquiring into different rotating speeds to the impact of citric acid output, uses different rotating speeds to carry out citric acid fermentation to the impact of citric acid output, and the result as shown in Figure 4.
As shown in Figure 4, when rotating speed is between the 100-200 r/min time, the output of citric acid increases to some extent, when rotating speed is in 200 r/min, citric acid output is for the highest, and when rotating speed surpasses 200 r/min, the output of citric acid does not increase, and therefore selecting rotating speed is 200 r/min.
Claims (5)
1. method of using Citric Acid Production by Immobilized Aspergillus niger is take alginate calcium as carrier embedded immobilization aspergillus niger cell, for the production of citric acid; It is characterized in that substratum is NH
4Cl 4 g/L MgSO
47H
2O 0.25 g/L, FeSO
40.25 mg/L CuSO
40.1 mg/L, ZnSO
40.1 mg/L, MnSO
40.2 mg/L.
2. method according to claim 1 is characterized in that, sucrose concentration is 120 g/L.
3. method according to claim 1 is characterized in that, temperature is 30 ℃.
4. method according to claim 1 is characterized in that, inoculum size 10%.
5. method according to claim 1 is characterized in that, rotating speed is 200 r/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2012103119140A CN102864184A (en) | 2012-08-29 | 2012-08-29 | Method for producing citric acid by utilizing immobilized aspergillus niger |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103119140A CN102864184A (en) | 2012-08-29 | 2012-08-29 | Method for producing citric acid by utilizing immobilized aspergillus niger |
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| CN102864184A true CN102864184A (en) | 2013-01-09 |
Family
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107418995A (en) * | 2017-07-26 | 2017-12-01 | 枣庄学院 | Ellagic acid prepared by a kind of granatanine liquid state fermentation and preparation method thereof |
| CN110106095A (en) * | 2019-04-04 | 2019-08-09 | 南京工业大学 | The aspergillus niger and its construction method and application that one plant of calcium channel CchA gene inactivates |
| CN110157603A (en) * | 2019-06-20 | 2019-08-23 | 南京高新工大生物技术研究院有限公司 | A kind of Immobilized Aspergillus niger produces the fermentation reactor and its method of citric acid |
-
2012
- 2012-08-29 CN CN2012103119140A patent/CN102864184A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 周定等: "固定化黑曲霉生产柠檬酸的研究", 《哈尔滨工业大学学报》 * |
| 柳萍等: "碳源对固定化黑曲霉生产柠檬酸影响的研究", 《食品与发酵工业》 * |
| 赵景联: "固定化黑曲霉Ax-6分生孢子生产柠檬酸的研究", 《西安交通大学学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107418995A (en) * | 2017-07-26 | 2017-12-01 | 枣庄学院 | Ellagic acid prepared by a kind of granatanine liquid state fermentation and preparation method thereof |
| CN107418995B (en) * | 2017-07-26 | 2019-12-03 | 枣庄学院 | A kind of ellagic acid and preparation method thereof of granatanine liquid state fermentation preparation |
| CN110106095A (en) * | 2019-04-04 | 2019-08-09 | 南京工业大学 | The aspergillus niger and its construction method and application that one plant of calcium channel CchA gene inactivates |
| CN110106095B (en) * | 2019-04-04 | 2020-08-04 | 南京工业大学 | Aspergillus niger genetically engineered bacterium with calcium ion channel CchA gene inactivated, and construction method and application thereof |
| CN110157603A (en) * | 2019-06-20 | 2019-08-23 | 南京高新工大生物技术研究院有限公司 | A kind of Immobilized Aspergillus niger produces the fermentation reactor and its method of citric acid |
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Application publication date: 20130109 |