CN102864101A - Acinetobacter calcoaceticus TA221 strain producing homoserine lactone and application thereof - Google Patents
Acinetobacter calcoaceticus TA221 strain producing homoserine lactone and application thereof Download PDFInfo
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- CN102864101A CN102864101A CN201210306033XA CN201210306033A CN102864101A CN 102864101 A CN102864101 A CN 102864101A CN 201210306033X A CN201210306033X A CN 201210306033XA CN 201210306033 A CN201210306033 A CN 201210306033A CN 102864101 A CN102864101 A CN 102864101A
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Abstract
The invention relates to acinetobacter calcoaceticus TA221 separated from an advanced plant. The strain can be used for producing homoserine lactone and controlling plant diseases, and belongs to the field of biotechnology. As proved by identification, the strain is an acinetobacter calcoaceticus strain, and is collected in the China General Microbiological Culture Collection Center with the collection number CGMCC No.4895. The strain can be well colonized on the surface of a plant in an application process, shows high stability and high continuity of control effects, and has a very good application prospect on the control of plant diseases.
Description
Technical field:
The present invention relates to biological technical field, relate to the acinetobacter calcoaceticus TA221 that can produce homoserine lactone that a strain is separated to the tobacco phyllosphere, and the application in the biological control field.
Background technology:
In modern agricultural production, use in a large number the way that the means such as chemical pesticide prevent Plant diseases, caused serious environmental pollution, and the ecological risk that thereupon produces is enough to cause people's concern.The high pesticide residue of Food Vegetable badly influence people's health, and the pollution of area source of abundant residues agricultural chemicals causes serious chain reaction in addition, and the ecological environmental pollution that brings is immeasurable.Harmful organism is carried out sustainable control and nuisanceless improvement receives people's concern day by day, and become an active research field, for Developing Sustainable Agriculture lays the foundation.
The transformation of the transgenic plant of disease resistance has reduced the dependence to chemical pesticide to a certain extent, has reduced the environmental pollution of chemical pesticide.Although some genetically modified crops has entered field experiment and progressively entered large-scale commercial applications, comparatively careful attitude is still held by majority state and government.Although ratified the plantation of other genetically modified crops in China, the ecological risk of genetically modified crops such as some problems such as spreading of: the horizontal transfer of gene, the variation of species diversity, genetically modified crops, is worth arousing enough attention.
Microbial pesticide is one of another kind of effective means of controlling the diseases such as plant, because microbial pesticide has certain environmental benefit and social benefit, positive support attitude is held by national governments, China particularly applies the aspect and has ranked among advanced international standard in Microbial resources exploitation, microbial pesticide kind, production technology and the scale of control Plant diseases.Yet in the formulation of microbial pesticide and qualitatively, we still have a certain distance with comparing abroad, particularly almost are blank in novel biocontrol product development field.
Summary of the invention:
The purpose of this invention is to provide a strain and produce the phyllospheric microorganism resource of homoserine lactone, another object of the present invention is to disclose the application of these Microbial resources in control of plant disease.
Acinetobacter calcoaceticus TA221 bacterial strain of the present invention, separation screening is from the tobacco plant blade face.The TA221 bacterial strain is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and deposit number is CGMCC No.4895, and Classification And Nomenclature is acinetobacter calcoaceticus (Acinetobacter calcoaceticus).Preservation date is on 05 25th, 2011, and the preservation address is No. 3 Institute of Microorganism, Academia Sinica in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
The application of acinetobacter calcoaceticus TA221 bacterial strain of the present invention is as follows:
1, substratum preparation:
1) culture presevation substratum: peptone peptone 10g, yeast soaks powder 5g, sodium-chlor 5g, agar 20g adds water to 1L, pH7.0-7.5;
2) fermention medium: peptone peptone 10g, yeast soaks powder 5g, sodium-chlor 5g, agar 20g adds water to 1L, pH7.0-7.5; Above substratum is all at 121 ℃ of sterilization 15-30min;
2, actication of culture: picking bacterial strain CGMCC No.4895 repeatedly rules 30 ℃ of overnight incubation to the culture presevation substratum; Picking list colony inoculation is in fermention medium, and 30 ℃, 200r/min cultivates 12-15h;
3, microbial inoculum preparation: with the bacterial classification liquid of step 2 activation, the inoculum size inoculation fermentation substratum according to 10%, 30 ℃, aerated culture 12-20h obtains the viable bacteria culture; Add the glycerine of mass fraction in the 2%-5% scope, canned preservation; Dilute with water is 100 times during use, sprays plant.
Embodiment:
The bacterial strain that the present invention uses, simple to operate, it can well be grown surely at plant surface, shows the duration of satisfactory stability and prevention effect.Below the present invention is further illustrated just in conjunction with specific embodiments, and purpose is to help the reader better to understand spirit of the present invention, but not as the restriction to the scope of the present invention.
Embodiment 1: the production of acinetobacter calcoaceticus TA221 microbial inoculum of the present invention:
1, substratum preparation:
1) culture presevation substratum: peptone peptone 10g, yeast soaks powder 5g, sodium-chlor 5g, agar 20g adds water to 1L, pH7.0-7.5;
2) fermention medium: peptone peptone 10g, yeast soaks powder 5g, sodium-chlor 5g, agar 20g adds water to 1L, pH7.0-7.5;
Above substratum is all at 121 ℃ of sterilization 15-30min;
2, actication of culture: picking bacterial strain CGMCC No.4895 repeatedly rules 30 ℃ of overnight incubation to the culture presevation substratum; Picking list colony inoculation is in fermention medium, and 30 ℃, 200r/min cultivates 12-15h;
3, microbial inoculum preparation: with the bacterial classification liquid of step 2 activation, the inoculum size inoculation fermentation substratum according to 10%, 30 ℃, aerated culture 12-20h obtains the viable bacteria culture; Add the glycerine of mass fraction in the 2%-5% scope, canned preservation.
Embodiment 2: the control that acinetobacter calcoaceticus TA221 does harm to wildfire:
Acinetobacter calcoaceticus TA221 is prepared into bacteria suspension (diluting 100 times microbial inoculum) and is sprayed on the tobacco leaf, naturally dries.According to conventional plant pathogenic bacteria inoculation method, the pseudomonas syringae (Pseudomonas syringae pv.tabaci) of inoculation different concns, each processes 3 repetitions, record incidence after the inoculation.Experimental result shows, inoculates pseudomonas syringae after 3 days, when dosage of inoculation is 10
5Caused during CFU/mL concentration that the control group tobacco produces strong pathology.And for the experimental group tobacco of having sprayed acinetobacter calcoaceticus TA221, when the inoculum size of pseudomonas syringae 10
7Tobacco shows faint pathology during CFU/mL concentration.Experimental result shows, acinetobacter calcoaceticus TA221 is obvious to the prevention effect of the false single bacterium of cloves.
Claims (2)
1. produce the acinetobacter calcoaceticus TA221 bacterial strain of homoserine lactone, it is characterized in that, this bacterial strain is a strain acinetobacter calcoaceticus (Acinetobacter calcoaceticus), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, deposit number: CGMCC No.4895.
2. with acinetobacter calcoaceticus TA221 bacterial strain controlling plant diseases claimed in claim 1, it is characterized in that, utilize the microbiobacterial agent controlling plant diseases of producing, specifically comprise:
(1) substratum preparation:
1) culture presevation substratum: peptone peptone 10g, yeast soaks powder 5g, sodium-chlor 5g, agar 20g adds water to 1L, pH7.0-7.5;
2) fermention medium: peptone peptone 10g, yeast soaks powder 5g, sodium-chlor 5g, agar 20g adds water to 1L, pH7.0-7.5; Above substratum is all at 121 ℃ of sterilization 15-30min;
(2) actication of culture: picking bacterial strain CGMCC No.4895 repeatedly rules 30 ℃ of overnight incubation to the culture presevation substratum; Picking list colony inoculation is in fermention medium, and 30 ℃, 200r/min cultivates 12-15h;
(3) microbial inoculum preparation: with the bacterial classification liquid of step (2) activation, the inoculum size inoculation fermentation substratum according to 10%, 30 ℃, aerated culture 12-20h obtains the viable bacteria culture; Add the glycerine of massfraction in the 2%-5% scope, the can preservation; Dilute with water is 100 times during use, sprays plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210306033XA CN102864101A (en) | 2012-08-24 | 2012-08-24 | Acinetobacter calcoaceticus TA221 strain producing homoserine lactone and application thereof |
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| CN201210306033XA CN102864101A (en) | 2012-08-24 | 2012-08-24 | Acinetobacter calcoaceticus TA221 strain producing homoserine lactone and application thereof |
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| CN201210306033XA Pending CN102864101A (en) | 2012-08-24 | 2012-08-24 | Acinetobacter calcoaceticus TA221 strain producing homoserine lactone and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103789223A (en) * | 2013-11-14 | 2014-05-14 | 西北大学 | Acinetobacter calcoaceticus with free living nitrogen fixation and phosphorus and potassium dissolving capability and application of acinetobacter calcoaceticus |
| CN108148774A (en) * | 2017-11-20 | 2018-06-12 | 中国烟草中南农业试验站 | Acinetobacter calcoaceticus, screening technique and the application of antagonism tobacco black shank bacterium |
| CN109042738A (en) * | 2018-08-31 | 2018-12-21 | 华南农业大学 | A kind of acinetobacter calcoaceticus and its application in the pathogenic pathogenic bacteria prevention and treatment of AHLs mediation |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102146348A (en) * | 2010-12-08 | 2011-08-10 | 中国科学院生态环境研究中心 | Homoserinelactone-producing acinetobacter calcoaceticus and application thereof |
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2012
- 2012-08-24 CN CN201210306033XA patent/CN102864101A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102146348A (en) * | 2010-12-08 | 2011-08-10 | 中国科学院生态环境研究中心 | Homoserinelactone-producing acinetobacter calcoaceticus and application thereof |
Non-Patent Citations (2)
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| 刘五星等: "《石油污染土壤的生态风险评价和生物修复*Ⅰ一株具有乳化石油能力的细菌分离鉴定》", 《土壤学报》 * |
| 徐玉泉等: "《一株苯酚降解菌的分离和鉴定》", 《环境科学学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103789223A (en) * | 2013-11-14 | 2014-05-14 | 西北大学 | Acinetobacter calcoaceticus with free living nitrogen fixation and phosphorus and potassium dissolving capability and application of acinetobacter calcoaceticus |
| CN103789223B (en) * | 2013-11-14 | 2017-02-08 | 西北大学 | Acinetobacter calcoaceticus with free living nitrogen fixation and phosphorus and potassium dissolving capability and application of acinetobacter calcoaceticus |
| CN108148774A (en) * | 2017-11-20 | 2018-06-12 | 中国烟草中南农业试验站 | Acinetobacter calcoaceticus, screening technique and the application of antagonism tobacco black shank bacterium |
| CN108148774B (en) * | 2017-11-20 | 2021-05-28 | 中国烟草中南农业试验站 | Acinetobacter calcoaceticus for antagonizing phytophthora parasitica, screening method and application |
| CN109042738A (en) * | 2018-08-31 | 2018-12-21 | 华南农业大学 | A kind of acinetobacter calcoaceticus and its application in the pathogenic pathogenic bacteria prevention and treatment of AHLs mediation |
| CN109042738B (en) * | 2018-08-31 | 2020-12-11 | 华南农业大学 | Acinetobacter and application thereof in prevention and treatment of pathogenic bacteria mediated by AHLs |
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Application publication date: 20130109 |