CN102854297A - Characterization method for atomic force microscope for testing quality of purified water - Google Patents
Characterization method for atomic force microscope for testing quality of purified water Download PDFInfo
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- CN102854297A CN102854297A CN2012103460050A CN201210346005A CN102854297A CN 102854297 A CN102854297 A CN 102854297A CN 2012103460050 A CN2012103460050 A CN 2012103460050A CN 201210346005 A CN201210346005 A CN 201210346005A CN 102854297 A CN102854297 A CN 102854297A
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Abstract
The invention relate to a characterization method for an atomic force microscope for testing the quality of purified water. The characterization method includes steps of 1, preparing a characterization sample and a material so that the characterization sample and the material are suitable to be observed via the atomic force microscope, namely preparing an evaporation substrate and cover glass assembly for the purified water to be tested and preparing the sample of the purified water to be tested; 2, modifying a characterization instrument so that the atomic force microscope is adaptive to the characterization sample; and 3, analyzing data of a characterization image and analyzing the characterization image to acquire useful information so as to determine the quality of the purified water. By the method, the quality of the purified water is tested completely, accurately and quickly, nanometer and micron-size impurities in the water are clearly and three-dimensionally displayed at one step, and the characterization method is significant in evaluating the quality of purified water for industry and commerce in aspects of science, economy and practicality.
Description
Technical field:
The present invention relates to the water quality detection method, be specifically related to a kind of atomic force microscope characterizing method of pure water water quality.
Background technology:
Be accompanied by human living standard's raising, people are to the requirement of quality of life, and are especially also more and more stricter to the requirement of potable water.Initial people get nature, and it is that potable water is drunk that the water of rivers and lakes directly is used as.Be accompanied by the quickening of industrialization paces, the appearance of various industry, people the waste discharge of various industry in rivers and lakes, so that fluviatic water no longer is fit to drink.So people begin to turn to underground water, because underground water is the product after the lithosphere filtration, so drink safer.But human speed of development was greatly improved in nearly decades, the waste material of various industry, waste water, scrapping components and parts etc. with various microelectronics has the thing of very big pollution also buried underground by people to the water source, underground water no longer is fit to quoting of people, therefore pure water just appears among the popular visual field, because the commercialization of pure water, the pure water brand on the market is multifarious especially, so that people are more and more fuzzyyer to the definition of pure water.Therefore the water quality detection of pure water is to ensure that people produce and the important measures of living safety.Yet pure water water quality detection method mainly contains the methods such as organoleptic detection, physics and chemistry detection, microorganism detection at present, unilateral, complicated, the shortcomings such as detection speed slow, detecting instrument, reagent costliness that these methods exist, the comprehensive express-analysis that affects pure water water quality is judged.
Summary of the invention:
In order to overcome and remedy the deficiency of existing pure water water quality detection method, the invention provides a kind of effective pure water water quality nanometer, micron order detection method, this disposable detection method makes pure water water quality detection process more complete, accurate, rapid, make nanometer and the clearly three-dimensional embodiment of micron-sized impurity in the water, to estimating the industry and commerce quality of pure water, have Scientific And Technical, economy and Practical significance.
The technical solution used in the present invention is: the atomic force microscope characterizing method of this pure water water quality detection comprises the following steps:
1. characterize the preparation of sample and material, so that its suitable atomic force microscope observation:
The preparation of ⅰ, pure water to be detected evaporation substrate-cover glass
18mm * 18mm standard cap slide is used first the above deionized water ultrasonic cleaning of 10M rank 10 minutes, repeat 5 times after changing water, then respectively clean 5 times with analyzing pure acetone and alcohol respectively, be kept in the closed container after drying, above operation requirements carries out in ultra-clean chamber more than ten thousand grades;
ⅱ, pure water sample preparation to be detected
Pure water to be detected is got with the micropipette rifle
Drip in cleaning on the dry good cover glass through step ⅰ, evenly be coated with out, and the good area that is coated with of survey record.The cover glass that coats pure water to be detected is put into vacuum drying chamber dry.Above operation requirements carries out in ultra-clean chamber more than ten thousand grades;
2. characterize the transformation of instrument, so that atomic force microscope can be fit to characterize sample:
Sample shallow storage space expansion with universal atomic force microscope makes its diameter greater than 3 centimetres, changes scanner head, makes sweep limit be
Above, mechanical processing method is widened the needle point seat, makes the sample shallow storage space of needle point seat greater than sample diameter, so that sample can be freely near needle point;
3. characterization and analysis of image data obtain useful information to determine water quality:
The cover glass of evaporation before and after the pure water placed respectively under the atomic force microscope be scanned into figure, the image that atomic force microscope is obtained is saved as 8 gray-scale maps, in order to embody Z direction True Data, grain size shape among the figure is carried out statistical study, thereby particle changes the kind of determining residuals in the water on the pure water front and rear covers slide to determine to be coated with, form, and then definite water quality, for objective description core sample more, with the average statistics of several figure drafting pattern as a result, comparative analysis characterizes residuals Statistical Distribution in the sample, and can be according to the area of the water that is coated with on cover glass, the volume of scan area and titration of straight water purification, can calculate pure water residual impurity distribution situation in the unit volume, above experimental implementation requires to carry out in ultra-clean chamber more than ten thousand grades.
The present invention has following beneficial effect: because the atomic force microscope that this programme has used nanoscale to characterize detects pure water water quality, overcome unilateral, complicated in the methods such as organoleptic detection, physics and chemistry detections, microorganism detection of present pure water water quality detection, detection speed slowly, the shortcomings such as detecting instrument, reagent costliness.And based on the pure water water quality detection method of atomic force microscopy, can quantitative, three-dimensional, comprehensive, the disposable sign of residual substance in the pure water, provide important scientific approach for differentiating industry and commerce with pure water.
Description of drawings:
Fig. 1 is that atomic force microscope characterizes certain commercial brand pure water image of selling on the market;
Fig. 2 analyzes certain commercial brand pure water of selling on the market with atomic force microscope images to increase the statistical graph that rear residual impurity changes with the uncork time.
Embodiment:
The invention will be further described below in conjunction with instantiation.
At first cover the fragmentation cleaning with the described method of i step of the step in the summary of the invention in 1., then water sample to be detected is carried out sample preparation by the described method of ii step of the step in the summary of the invention in 1..Next is to carry out the atomic force microscope transformation, its suitable reservoir core micropore structure is characterized, the AJ-III type atomic force microscope that the atomic force microscope that the present invention uses is produced as Shanghai AJ Nano-Science Development Co., Ltd., utilize machining to expand the sample shallow storage space, making its diameter is 3.0cm, change scanner head, make sweep limit be
, utilize machining process to widen the needle point seat, make needle point seat sample shallow storage space enough large (greater than the sample breadth extreme), so that sample can be freely near needle point (if above transformation is finished, this step can be omitted).Carry out surface topography at improved atomic force microscope and observe, the observation of cover glass after comprising the morphology observation of Tu Shuiqian cleaning cover glass and being coated with the water drying in order to residual substance actual content in the pure water relatively, and is preserved into the tif form with token image.
Graphical analysis: the image that atomic force microscope is obtained is saved as 8 gray-scale maps, in order to embody Z direction True Data.Pure water typical case afm scan image as shown in Figure 1.Left figure among Fig. 1 is the feature image that is coated with the front cover glass of water after the cleaning, and sweep limit is
, other gray scale limit vitta represents height, and can see does not have larger particles in publishing picture, and image is very smooth, only has the trickle cut on the cover glass; Middle figure among Fig. 1 is the dried atomic force microscope feature image of certain brand pure water, and sweep limit is
, can see the different particle of middle distribution size and shape of publishing picture; Right figure among Fig. 1 is the stereographic map of middle figure, figure and right figure height in the representative of next door gray scale vitta.
For the sample that objective description more detects, the particle among Fig. 1 is added up drafting pattern 2.Statistics shows that the particle less than 200nm that this brand pure water presents among every width of cloth figure is 16 after just opening, along with open-interval increases, the granule number in this scope increases to some extent and tends to be steady, although during beginning greater than
Particle in water, do not find, but began after (96 hours) to occur at four days, bacterial number begins to roll up in this explanation water, has been unsuitable for drinking.Because recording image is cover glass surface three dimension True Data, so more careful quantitative analysis can directly be finished by image.It is that other characterization methods and method are difficult to obtain that the present invention analyzes the conclusion that draws, and illustrates that the present invention has extraordinary advantage and effect in sign pure water water quality.
Claims (1)
1. the atomic force microscope characterizing method of a pure water water quality detection comprises the following steps:
1. characterize the preparation of sample and material, so that its suitable atomic force microscope observation:
The preparation of ⅰ, pure water to be detected evaporation substrate-cover glass
18mm * 18mm standard cap slide is used first the above deionized water ultrasonic cleaning of 10M rank 10 minutes, repeat 5 times after changing water, then respectively clean 5 times with analyzing pure acetone and alcohol respectively, be kept in the closed container after drying, above operation requirements carries out in ultra-clean chamber more than ten thousand grades;
ⅱ, pure water sample preparation to be detected
Pure water to be detected is got with the micropipette rifle
Drip in cleaning on the dry good cover glass through step ⅰ, evenly be coated with out, and the good area that is coated with of survey record, the cover glass that coats pure water to be detected is put into vacuum drying chamber dry, above operation requirements carries out in ultra-clean chamber more than ten thousand grades;
2. characterize the transformation of instrument, so that atomic force microscope can be fit to characterize sample:
Sample shallow storage space expansion with universal atomic force microscope, make its diameter greater than 3 centimetres, change scanner head, make sweep limit be 60 microns * more than 60 microns, mechanical processing method is widened the needle point seat, make the sample shallow storage space of needle point seat greater than sample diameter, so that sample can be freely near needle point;
3. characterization and analysis of image data obtain useful information to determine water quality:
The cover glass of evaporation before and after the pure water placed respectively under the atomic force microscope be scanned into figure, the image that atomic force microscope is obtained is saved as 8 gray-scale maps, in order to embody Z direction True Data, grain size shape among the figure is carried out statistical study, thereby particle changes the kind of determining residuals in the water on the pure water front and rear covers slide to determine to be coated with, form, and then definite water quality, for objective description core sample more, with the average statistics of several figure drafting pattern as a result, comparative analysis characterizes residuals Statistical Distribution in the sample, and can be according to the area of the water that is coated with on cover glass, the volume of scan area and titration of straight water purification, can calculate pure water residual impurity distribution situation in the unit volume, above experimental implementation requires to carry out in ultra-clean chamber more than ten thousand grades.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114113080A (en) * | 2021-10-11 | 2022-03-01 | 四川轻化工大学 | Method for identifying aroma type and quality of white spirit |
Citations (4)
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| CN1546984A (en) * | 2003-12-12 | 2004-11-17 | 上海爱建纳米科技发展有限公司 | Nanometer-particle microscope |
| CN1987416A (en) * | 2006-12-27 | 2007-06-27 | 浙江大学 | Method and device for measuring trace matter in liquid by micro suspension arm sensor |
| US20080044924A1 (en) * | 2006-08-21 | 2008-02-21 | Kurita Water Industries Ltd. | Water quality evaluation method and substrate contacting apparatus used |
| CN102183450A (en) * | 2011-04-20 | 2011-09-14 | 东北石油大学 | Characterization method of atomic force microscope for micro-pore structure of reservoir rock core |
-
2012
- 2012-09-18 CN CN2012103460050A patent/CN102854297A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1546984A (en) * | 2003-12-12 | 2004-11-17 | 上海爱建纳米科技发展有限公司 | Nanometer-particle microscope |
| US20080044924A1 (en) * | 2006-08-21 | 2008-02-21 | Kurita Water Industries Ltd. | Water quality evaluation method and substrate contacting apparatus used |
| CN1987416A (en) * | 2006-12-27 | 2007-06-27 | 浙江大学 | Method and device for measuring trace matter in liquid by micro suspension arm sensor |
| CN102183450A (en) * | 2011-04-20 | 2011-09-14 | 东北石油大学 | Characterization method of atomic force microscope for micro-pore structure of reservoir rock core |
Non-Patent Citations (3)
| Title |
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| 史智平: "《原子力显微镜对水中颗粒物的检测》", 《唐山师范学院学报》 * |
| 汤鸿霄等: "《环境纳米污染物与微界面水质过程》", 《环境科学学报》 * |
| 葛小鹏等: "《原子力显微镜在环境样品研究与表征中的应用于展望》", 《环境科学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114113080A (en) * | 2021-10-11 | 2022-03-01 | 四川轻化工大学 | Method for identifying aroma type and quality of white spirit |
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