CN102851248B - Lactobacillus jensenii for preventing and curing bacterial vaginosis - Google Patents
Lactobacillus jensenii for preventing and curing bacterial vaginosis Download PDFInfo
- Publication number
- CN102851248B CN102851248B CN2012103477155A CN201210347715A CN102851248B CN 102851248 B CN102851248 B CN 102851248B CN 2012103477155 A CN2012103477155 A CN 2012103477155A CN 201210347715 A CN201210347715 A CN 201210347715A CN 102851248 B CN102851248 B CN 102851248B
- Authority
- CN
- China
- Prior art keywords
- lactobacillus jensenii
- imj
- lactobacillus
- 1cgmcc
- jensenii
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses lactobacillus jensenii and application thereof. The lactobacillus jensenii is particularly lactobacillus jensenii IMJ-1, and the file No. of the lactobacillus jensenii IMJ-1 in the General Microbiological Culture Collection Center of China Microbial Culture Collection Management Committee is CGMCC No. 6558. The lactobacillus jensenii IMJ-1 CGMCC No.6558 has high H2O2 generation capability, can effectively suppress breeding of external pathogenic bacteria and other conditional pathogenic bacteria, and has a high binding function to epithelial cells of vagina, and accordingly, breeding of bacteria strains on the vaginal epithelium is guranteed. Besides, the lactobacillus jensenii IMJ-1 CGMCC No. 6558 has a certain application prospect in preventing and curing bacterial vaginosis.
Description
Technical field
The invention belongs to biological technical field, relate to Lactobacillus Jensenii and application thereof, particularly a strain separates the Lactobacillus Jensenii that is used for the control bacterial vaginosis from human body.
Background technology
Microorganism perches at human body each position almost, all exist a large amount of bacteriums such as each positions such as skin, oral cavity, enteron aisle, reproductive tracts, comprising multiple different types of bacterium, can be divided into roughly " probiotics " and " condition cure the disease bacterium " two large classes, keep stable equilibrium state in quantity and the ratio of these bacteriums of healthy human body.Can have various bacteria in the woman vagina, wherein, lactobacillus is to keep the most common and most important probiotics of vaginal health micro-ecological environment, and lactobacillus accounts for more than 90% of healthy women vaginal bacteria total amount.They are mainly by producing the growth of acid, hydrogen peroxide inhibition pathogenic bacteria and other conditioned pathogens.If some factor causes intravaginal Bacterium lacticum quantity and reduces, even complete disappearance, then can cause the many conditions of the intravaginal bacterium abnormality proliferation of curing the disease, the bacterial vaginosis take obvious microecology in vaginas environment disturbance as principal character appears.
Bacterial vaginosis is the most common but frequently-occurring disease that do not come into one's own of Women of Childbearing Age, and the morbidity in healthy women is about 20%, and the pregnant woman is about 15%.Bacterial vaginosis is " little disease, burden ", and patients ' life quality obviously descends, and mental burden is very heavy; And the risk of the venereal disease such as aids infection, gonorrhoea, nongonococcal urethritis obviously increases; A series of pregnancy complications can appear in ill pregnant woman, such as premature labor and chorioamnionitis etc.At present, the treatment of bacterial vaginosis mainly relies on the microbiotic such as metronidazole or lincomycin, but result for the treatment of is not good, and curative ratio is less than 50%, and the ratio of recurrence is high.Even half patient widely applies microbiotic also can not cure, the clinical symptom sustainable existence, the patient enjoys and decocts, and the doctor suffers from not having good plan.How Control in recurring, thoroughly to effect a radical cure bacterial vaginosis be the thorny problem that the gynecologist needs solution badly.
In order to improve the result for the treatment of of bacterial vaginosis, be badly in need of clinically new treatment plan.Because the toxic side effect such as the liver that antibiotic therapy bacterial vaginosis effect is not good enough and antibiotic therapy causes, renal impairment are making great efforts to explore the little ecological therapy based on the probiotic bacterium Lemonal both at home and abroad always.Microecologic cure is to become the increase probiotic bacterium take killing microorganisms as main methods for the treatment of, to recover the novel therapeutic theory that the normal micro-ecological environment of vagina is purpose.Namely after utilizing microbiotic to remove pathogenic bacterium, directly replenish the living preparation of lactobacillus preparation that separates from the healthy women vagina, its Colonization is got off, promote the ability of the opposing pathogenic bacterium growth that it is natural.
There is multiple Bacterium lacticum in the healthy women intravaginal, has individual difference, and anti-pathogenic bacterium capacity variance is obvious between each strain of Lactobacillus Jensenii.When selecting the Bacterium lacticum probiotic bacterium, need to consider the kind of Bacterium lacticum, it produces acid, produces H
2O
2Ability, and the ability that adheres to vaginal epithelial cell, wherein can lactobacillus be the basis of Bacterium lacticum continuous action in vagina success field planting, also is the key factor of Bacterium lacticum performance curative effect.
Because different lactobacillus strainss has different adhesion avidity to vaginal epithelial cell, the bacterial strain colonization ability that avidity is strong is strong, just can play the effect of killing multiple pathogenic microorganisms that presses down.If the living preparation of lactobacillus preparation can not field planting at vaginal epithelial cell, in the experiment in vitro the stronger effect of killing multiple pathogenic microorganisms that presses down all do not know where to begin, become kite bill.
One is improved and effective probiotic bacterium bacterial classification, and itself must possess following standard at least: (1) must be the normal field planting bacterium of host's application site; (2) can be in the survival of host's application site and growth; (3) can provide the host useful effect; (4) related products is made and must can be kept activity and survival ability between the shelf lives.
At present, state's per vaginam probiotics bacterial agent only has a kind of, and its main component is the lactobacillus delbruckii viable bacteria.Although it is a kind of Bacterium lacticum of separating from the healthy women vagina, not the main probiotics in China's woman vagina flora, China's healthy women vaginal bacteria is take Lactobacillus Jensenii as main.
Summary of the invention
The purpose of this invention is to provide the Lactobacillus Jensenii that a strain is used for the control bacterial vaginosis.
Lactobacillus Jensenii provided by the present invention is specially Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1, this bacterium is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on September 11st, 2012 and (is called for short CGMCC, the address is: the Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), preservation registration number is CGMCC No.6558.
Described Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 size is the oyster white bacterium colony of 1-2 millimeter, opaque, projection approximately, colony edge neatly, more moistening, size and form is stablized (Fig. 1).Be in the described bacterial strain IMJ-1 of logarithmic phase, through smear, gram's staining, the form of optical microphotograph Microscopic observation thalline is: gram's staining is positive, bactacin, atrichia.(Fig. 2).Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 amphimicrobian, 37-42 ℃ of growth optimum temperuture, optimal pH 5.8-6.2; But the multiple sugar such as glucose fermentation, fructose, sucrose, trehalose, maltose.
Described Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 is producing H
2O
2In application also belong to protection scope of the present invention.
In described application, behind substratum, adopt the mode that leaves standstill first again shaking culture to cultivate described Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 kind; Leave standstill to cultivate and be anaerobic environment.
In one embodiment of the invention, described substratum is specially MRS (deMan, Rogosa and Sharpe, MRS) liquid nutrient medium; The temperature of described cultivation is 37 ℃; Described leaving standstill first cultivated shaking culture again and is specially to leave standstill first and cultivates again shaking culture 3h of 21h; The rotating speed of described shaking culture is 220rpm, and rotation radius is 2.54cm.
Described Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 also belongs to protection scope of the present invention in the application that preparation prevents and/or treats in the product of bacterial vaginosis.
Described product can be microbial inoculum or the medicine that prevents and/or treats bacterial vaginosis.
Described Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 also belongs to protection scope of the present invention in the application that preparation has in the product of vaginal epithelial cell adhesion function.
A further object of the present invention provides a kind of microbial inoculum.
Microbial inoculum provided by the present invention is the microbial inoculum take described Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCCNo.6558 as activeconstituents.
Described microbial inoculum is being produced H
2O
2In application also belong to protection scope of the present invention.
Described microbial inoculum also belongs to protection scope of the present invention in the application that preparation prevents and/or treats in the product of bacterial vaginosis.
Described microbial inoculum also belongs to protection scope of the present invention in the application that preparation has in the product of vaginal epithelial cell adhesion function.
Another purpose of the present invention provides a kind of product that prevents and/or treats bacterial vaginosis.
The activeconstituents that prevents and/or treats the product of bacterial vaginosis provided by the present invention is described Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558.
Described product can be microbial inoculum or the medicine that prevents and/or treats bacterial vaginosis.
Another purpose of the present invention provides a kind of product with vaginal epithelial cell adhesion function.
Activeconstituents with product of vaginal epithelial cell adhesion function provided by the present invention is described Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558.
The present invention is according to the recognised standard of probiotic bacterium bacterial classification, from intravaginal dominant microflora-Lactobacillus Jensenii, and therefrom filtered out a strain and has higher product H in China's healthy women body
2O
2Ability and probiotic strain-Lactobacillus Jensenii (Lactobacillus jensenii) the IMJ-1CGMCC No.6558 strong with vaginal epithelial cell Colonization ability.Experiment showed, with described Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558, according to 10
6The density of cfu/ml is inoculated in the MRS liquid nutrient medium, in anaerobic environment lower 37 ℃ leave standstill cultivate 21h after, be converted to shaking culture 3h, rotating speed is 220rpm, H in the final gained nutrient solution supernatant
2O
2Concentration up to 362.3 μ M.Efficient produce hydrogen peroxide, but the external pathogenic bacteria of establishment and the growth of other conditioned pathogens; The stronger adhesive attraction of vaginal epithelial cell has been guaranteed the field planting of bacterial strain at vagina epithelium.Lactobacillus Jensenii provided by the present invention (Lactobacillus jensenii) IMJ-1CGMCC No.6558 has certain application prospect at bacterial vaginosis aspect preventing and treating.
The preservation explanation
Strain name: Lactobacillus Jensenii
Latin name: (Lactobacillus jensenii)
Strain number: IMJ-1
Preservation mechanism: China Committee for Culture Collection of Microorganisms common micro-organisms center
Preservation mechanism is called for short: CGMCC
Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
Preservation date: on September 11st, 2012
The preservation center numbering of registering on the books: CGMCC No.6558
Description of drawings
Fig. 1 is Lactobacillus Jensenii (Lactobacillus jensenii) the IMJ-1CGMCC No.6558 bacterium colony that separates from the Healthy People guiding.
Fig. 2 is the thalli morphology of optical microphotograph Microscopic observation Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558.
Fig. 3 is the adhesive attraction of Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 and vaginal epithelial cell.
Embodiment
Employed experimental technique is ordinary method if no special instructions among the following embodiment.
Used material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
The separation of embodiment 1, Lactobacillus Jensenii bacterial strain and evaluation
One, the separation of Lactobacillus Jensenii bacterial strain
MRS substratum: peptone 10.0g; Extractum carnis 10.0g; Yeast extract paste 5.0g; Diammonium hydrogen citrate 2.0g; Glucose 20.0g; Tween 80,1.0mL; Sodium acetate (CH
3COONa3H
2O) 5.0g; Dipotassium hydrogen phosphate (K
2HPO43H
2O) 2.0g; Sal epsom (MgSO
47H
2O) 0.58g; Manganous sulfate (MnSO
4H
2O) 0.25g; Distilled water 1000mL; Transfer to pH 6.2 ~ 6.6.
Collected the healthy women vaginal secretion matter sample of more than 200 routine clinical detection absence of vagina inflammation shape signs, with the sample number consecutively, be inoculated in the MRS liquid nutrient medium, 37 ℃ of anaerobism are cultivated 24h.5 single bacterium colonies of each sample random choose are cultivated in 2mL MRS liquid nutrient medium and are increased bacterium 24h, and to the bacterium colony number consecutively.The amplification bacterium is used for bacterium conservation and DNA preparation.With the hundreds of strain bacteriums that are separated to carry out respectively strain identification, produce hydrogen peroxide and with the detection of vaginal epithelial cell adhesive attraction, a strain is wherein had the Lactobacillus Jensenii called after IMJ-1 of stronger adhesive attraction and product hydrogen peroxide.
Two, the evaluation of Lactobacillus Jensenii bacterial strain
Through identifying, it is Lactobacillus Jensenii (Lactobacillus jensenii) that step 1 is separated the bacterial strain IMJ-1 that obtains.This Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1 is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on September 11st, 2012 and (is called for short CGMCC, the address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), deposit number is CGMCC No.6558.Specifically from following several respects bacterial strain IMJ-1 is identified:
1, Morphological Identification
On MRS lactobacillus culture medium flat plate, above-mentioned steps one is separated the oyster white bacterium colony that the bacterial strain IMJ-1 that obtains shows as big or small approximately 1-2 millimeter, opaque, projection, and colony edge neatly, more moistening, size and form is stablized (Fig. 1).
Be in the described bacterial strain IMJ-1 of logarithmic phase, through smear, gram's staining, the form of optical microphotograph Microscopic observation thalline is: gram's staining is positive, bactacin, atrichia.(Fig. 2).
2, analysis of physio biochemical characteristics
Bacterial strain IMJ-1 amphimicrobian, 37-42 ℃ of growth optimum temperuture, optimal pH 5.8-6.2.Utilize API 50CHL substratum and API 50CH reagent strip (bioMe ' rieux according to API Bacteria Identification standard, Inc., Marcy l ' Etoile, France) carry out the physiological and biochemical property that sugar-fermenting reaction interpretation detects bacterial strain IMJ-1 to detecting bacterial strain IMJ-1.The simple culture medium that API50CHL is comprised of the API 50CH test bar of 49 kinds of fermentable carbohydrates.Make each tubule of suspension inoculation test bar to measure bacterium.When cultivating, because fermentable carbohydrates is produced acid, pH descends, and makes indicator discoloration.
The result shows, but the multiple sugar such as bacterial strain IMJ-1 glucose fermentation, fructose, sucrose, trehalose, maltose.
3,16s rDNA sequence homology analysis
Ordinary method is cultivated above-mentioned steps one and is separated the bacterial strain IMJ-1 that obtains, extract total DNA of bacterial strain as the gene amplification template, adopt universal primer 27f:5 '-AGAGTTTGATCCTGGCTCAG-3 ', 1492r:5 '-TACGGTTACCTTGTTACGACTT-3 ' amplification bacterial 16 S rRNA gene conserved regions.25 μ L amplification systems comprise: 1 * PCR reaction buffer, 200 μ mol/L dNTPs, each 0.2 μ mol/L of upstream and downstream primer, 1U Taq archaeal dna polymerase, 1 μ L template DNA.Reaction conditions: 94 ℃ of denaturation 5min; 94 ℃ of sex change 30s, 55 ℃ of annealing 40s, 72 ℃ are extended 30s, totally 25 circulations; 72 ℃ are extended 10min.PCR product 1% detected through gel electrophoresis, positive findings 27f:5 '-AGAGTTTGATCCTGGCTCAG-3 ', 1492r:5 '-TACGGTTACCTTGTTACGACTT-3 ' carries out two-way order-checking.Sequence assembly and similarity analysis use DNAStar software to finish, and sequence alignment is finished online by American National biotechnology information center ncbi database (http://www.ncbi.nlm.nih.gov).
The sequence of the 16s rDNA of bacterial strain IMJ-1 sees sequence 1 in the sequence table for details.
The functional analysis of embodiment 2, Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558
1, Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 produces H
2O
2Detection H
2O
2Standard curve making: at first be piperazine-N of 100mmol/L with concentration, N'-two-ethyl sulfonic acid (Piperazine-N, N_-Bis 2-ethanesulfonic acid, PIPES) is with 30% (v/v) H
2O
2(being equivalent to 9.128mol/L) storage liquid is diluted to 1mol/L, uses 100mmol/L PIPES with 1mol/L H again
2O
2Be diluted to respectively the working fluid of 0 μ mol/L, 20 μ mol/L, 40 μ mol/L, 60 μ mol/L, 80 μ mol/L, 100 μ mol/L; Then get respectively the above-mentioned H of 100 μ L
2O
2Working fluid is the TMB(tetramethyl benzidine of 20mmol/L with 100 μ L concentration), 2 μ L horseradish peroxidases (1mg/mL) mix, and hatch 10min for 16 ℃, measure OD
630Value.Finally obtain H
2O
2Concentration standard curve: y=0.0033x-0.0496, R
2=0.992.
Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 liquid culture H
2O
2Concentration detects: picking Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 bacterium colony, and the bacterium liquid that inoculation 1mL cultivates 48h is in 10mL MRS liquid nutrient medium, and the concentration that makes bacterial strain in the nutrient solution is 10
6Cfu/ml, in anaerobic environment lower 37 ℃ leave standstill cultivate 21h after, be converted to 37 ℃ of shaking culture 3h, rotating speed is 220r/min, rotation radius is 2.54cm, cultivate finish after, collect fermented liquid, the centrifugal 2min of 12000r/min gets 100 μ L bacterium liquid supernatants and measures OD
630Value according to typical curve, is calculated H in the bacterium liquid supernatant
2O
2Concentration.The experiment triplicate, results averaged.
The result is as shown in table 1, and Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 can produce a large amount of H in fermentation culture
2O
2, its concentration is up to 362.3 μ M.
Table 1 Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 produces H
2O
2Ability detect unit: μ M
| Repeat 1 | Repeat 2 | Repeat 3 | Mean value | |
| H 2O 2Output | 300.8 | 378.0 | 408.1 | 362.3 |
2, Lactobacillus Jensenii is to the adhesive attraction of vaginal epithelial cell
The vagina epithelium cast-off cells are taken from more than 200 examples healthy (clinical detection shows that non-reproductive system infects) Women of Childbearing Age, add respectively MEM substratum (pH 4.0) behind each cell sample label, centrifugal 10 minutes of 120g, repeated centrifugation is 2 times again, wash surface bacteria off, adjusting vagina epithelium cast-off cells concentration with the MEM substratum is 10
5Individual/mL; It is (10 that activation incubated overnight Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 makes its concentration
8CFU/mL).Each 500 μ L equal-volume of described vagina epithelium cast-off cells and described Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 mix and are placed on 37 ℃ of shaking tables (rotating speed is 100r/min, rotation radius is 2.54cm) hatch 1h, then use 0.8 μ m membrane filtration, remove free Lactobacillus Jensenii (Lactobacillusjensenii) IMJ-1CGMCC No.6558, and gently be pressed on filter membrane on the cover glass, fixed cell carries out oily sem observation behind the gramstaining, count respectively three cell adhesion bacterial counts, calculate each cell and on average adhere to bacterial count.Adopt equally the method isolation identification of embodiment 1 to obtain an other strain simultaneously, but Lactobacillus Jensenii (Lactobacillus jensenii) the conduct negative contrast weak with vaginal epithelial cell Colonization power.
Result's demonstration, Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 all has stronger adhesive attraction to the vaginal epithelial cell that separates from more than 200 routine healthy Women of Childbearing Ages.Fig. 3 is oily sem observation figure behind the adhesive attraction gramstaining of two different strain Lactobacillus Jenseniis of Colonization power and vaginal epithelial cell, and the figure first half is the vagina epithelium cast-off cells that are located away from healthy Women of Childbearing Age about being; The figure Lower Half is vagina epithelium cast-off cells Lactobacillus Jensenii adhesive attraction, and lower-left figure represents stronger adhesion (Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558), and bottom-right graph represents weak adhesion (negative contrast).More than 200, the example, choose at random 4 examples, carry out statistical study, calculate the quantity that each vaginal epithelial cell on average adheres to Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 and reach 243 (seeing Table 2).
Table 2 vaginal epithelial cell adheres to the quantity statistics of Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558
The result of comprehensive embodiment 1 and embodiment 2, Lactobacillus Jensenii of the present invention (Lactobacillus jensenii) IMJ-1CGMCC No.6558 had both had stronger generation H
2O
2Ability (but growth of establishment pathogenic bacteria and other conditioned pathogens), have again the stronger adhesive attraction to vaginal epithelial cell (having guaranteed the field planting of bacterial strain at vagina epithelium), this is just so that Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1CGMCC No.6558 becomes candidate's probiotic strain of effective control bacterial vaginosis.
Claims (8)
1. Lactobacillus Jensenii (Lactobacillus jensenii) IMJ-1, its deposit number at China Committee for Culture Collection of Microorganisms common micro-organisms center is CGMCC No.6558.
2. the described Lactobacillus Jensenii of claim 1 (Lactobacillus jensenii) IMJ-1CGMCC No.6558 prevents and/or treats application in the product of bacterial vaginosis in preparation.
3. the described Lactobacillus Jensenii of claim 1 (Lactobacillus jensenii) IMJ-1CGMCC No.6558 has application in the product of vaginal epithelial cell adhesion function in preparation.
4. the microbial inoculum take the described Lactobacillus Jensenii of claim 1 (Lactobacillus jensenii) IMJ-1CGMCC No.6558 as activeconstituents.
5. the described microbial inoculum of claim 4 prevents and/or treats application in the product of bacterial vaginosis in preparation.
6. the described microbial inoculum of claim 4 has application in the product of vaginal epithelial cell adhesion function in preparation.
7. prevent and/or treat the product of bacterial vaginosis, it is characterized in that: the activeconstituents of described product is Lactobacillus Jensenii claimed in claim 1 (Lactobacillus jensenii) IMJ-1CGMCC No.6558.
8. have the product of vaginal epithelial cell adhesion function, it is characterized in that: the activeconstituents of described adhesion product is Lactobacillus Jensenii claimed in claim 1 (Lactobacillus jensenii) IMJ-1CGMCC No.6558.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103477155A CN102851248B (en) | 2012-09-18 | 2012-09-18 | Lactobacillus jensenii for preventing and curing bacterial vaginosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103477155A CN102851248B (en) | 2012-09-18 | 2012-09-18 | Lactobacillus jensenii for preventing and curing bacterial vaginosis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102851248A CN102851248A (en) | 2013-01-02 |
| CN102851248B true CN102851248B (en) | 2013-10-23 |
Family
ID=47398228
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012103477155A Active CN102851248B (en) | 2012-09-18 | 2012-09-18 | Lactobacillus jensenii for preventing and curing bacterial vaginosis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102851248B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293718B (en) * | 2014-10-11 | 2017-01-18 | 中国科学院微生物研究所 | Lactobacillus jensenii and application thereof |
| CN104694414B (en) * | 2014-11-21 | 2019-05-24 | 北京百益恒源科技有限公司 | One plant of Zhan Shi Bacillus acidi lactici (Lactobacillus jensenii) and its pharmaceutical applications |
| CN104694413B (en) * | 2014-11-21 | 2018-04-17 | 北京百益恒源科技有限公司 | One plant of lactobacillus curvatus（Lactobacillus crispatus）And its pharmaceutical applications |
| CN110540945B (en) * | 2018-05-29 | 2023-04-25 | 广东强基药业有限公司 | Lactobacillus jensenii and application thereof in preparing vaginal antibacterial drugs |
| CN110656060B (en) | 2019-08-09 | 2020-08-07 | 四川厌氧生物科技有限责任公司 | Multi-linked lactobacillus composition and application thereof in female vaginal health |
| CN114350561B (en) * | 2022-01-05 | 2023-06-13 | 江南大学 | Lactobacillus jensenii for relieving inflammation caused by gardnerella vaginalis and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1184637A (en) * | 1996-12-07 | 1998-06-17 | 大连医科大学科达药业有限公司 | Lactobacillus and prepn. contg. same for treating vaginopathy |
| SE528382C2 (en) * | 2004-10-05 | 2006-10-31 | Probi Ab | Probiotic lactobacillus strains for improved vaginal health |
-
2012
- 2012-09-18 CN CN2012103477155A patent/CN102851248B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102851248A (en) | 2013-01-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102851248B (en) | Lactobacillus jensenii for preventing and curing bacterial vaginosis | |
| CN108004187A (en) | A kind of lactobacillus gasseri and its application for being used to prepare vagina antibacterial medicines | |
| TW201531560A (en) | Lactobacillus crispatus and application thereof | |
| CN104178437A (en) | Lactobacillus crispatus and its application in gynecological diseases | |
| CN1888051A (en) | Plant lactobacillus strain and its application | |
| CN103911305B (en) | Lactobacillus crispatus bacterial strain and uses thereof | |
| CN103421715A (en) | Lactobacillus rhamnosus and application thereof | |
| CN103074270A (en) | Lactobacillus crispatus and application | |
| CN103911306B (en) | Lactobacillus gasseri bacterial strain and application thereof | |
| CN103275893A (en) | Complex microbial inoculants of bifidobacterium longum and bifidobacterium animalis and preparation method of complex microbial inoculants | |
| CN108004188A (en) | A kind of Lactobacillus rhamnosus and its application for being used to prepare vagina antibacterial medicines | |
| CN104232537B (en) | Lactobacillus crispatus and application thereof | |
| CN105586299A (en) | Lactobacillus acidophilus preparation and application thereof | |
| CN103409334A (en) | Lactobacillus for inhibiting pathogenic bacteria of vaginitis and application thereof | |
| CN111100810A (en) | Lactobacillus plantarum DNB1, and extracellular polysaccharide and application thereof | |
| CN103039710B (en) | Method for producing camellia oil cake feed through mixed strain fermentation | |
| CN103911308A (en) | Lactobacillus crispatus strain and its uses | |
| CN104673702B (en) | One plant of lactobacillus curvatus（Lactobacillus crispatus）And its pharmaceutical applications | |
| CN103911309B (en) | Lactobacillus gasseri bacterial strain and application thereof | |
| CN111040959A (en) | Grass carp source lactic acid bacteria with specific pathogenic microorganism antagonistic capability and application thereof | |
| CN104293718B (en) | Lactobacillus jensenii and application thereof | |
| CN102008110A (en) | Multi-strain microbial composite beverage preparation and preparation method thereof | |
| CN103911307B (en) | Lactobacillus crispatus bacterial strain and uses thereof | |
| CN105349474A (en) | Lactobacillus.crispatus for preventing female urogenital tract infection | |
| CN104694414B (en) | One plant of Zhan Shi Bacillus acidi lactici (Lactobacillus jensenii) and its pharmaceutical applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20181009 Address after: 102206 Floor No. 6 - 2 to Floor 101, Zone 1, No. 8, Life Park Road, Zhongguancun Life Science Park, Huilongguan Town, Changping District, Beijing (Room 413, 4 stories) Patentee after: Beijing Yisheng garden Biotechnology Co., Ltd. Address before: No. 3, No. 1, Beichen West Road, Beichen, Beijing Patentee before: Institute of Microbiology, Chinese Academy of Sciences |
|
| TR01 | Transfer of patent right |