CN102847951A - Process for preparing gold nano particles through reduction of chloroauric acid by catalase - Google Patents
Process for preparing gold nano particles through reduction of chloroauric acid by catalase Download PDFInfo
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- CN102847951A CN102847951A CN2012102589223A CN201210258922A CN102847951A CN 102847951 A CN102847951 A CN 102847951A CN 2012102589223 A CN2012102589223 A CN 2012102589223A CN 201210258922 A CN201210258922 A CN 201210258922A CN 102847951 A CN102847951 A CN 102847951A
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Abstract
The invention discloses a process for preparing gold nano particles through reduction of chloroauric acid by catalase. The process comprises the steps of dropping a catalase solution into a chloroauric acid solution, and mixing uniformly; and adjusting the pH value of a mixed solution to be alkaline, reacting on the water bath condition of 20-37 DEG C, and obtaining gold nano particles through separation after the reaction is completed. According to the process, the catalase is introduced to serve as a reducing agent and a protective agent, reducing functional groups on the catalase are high in reducibility on the alkaline condition, the synthesis of gold nano particles is facilitated, and the produced gold nano particles are free from agglomeration on the high salinity condition (0.5MNaCl).
Description
Technical field
The present invention relates to a kind of preparation method of golden nanometer particle, relate in particular to the method that a kind of catalase reduction tetra chlorauric acid prepares golden nanometer particle.
Background technology
Golden nanometer particle refers to that particle diameter is at the particulate of 1～100nm.Golden nanometer particle has special skin effect, bulk effect, quantum size effect and macro quanta tunnel effect, in a plurality of fields such as catalysis, electronics and optics, biotechnologys important application is arranged.Golden nanometer particle has an absworption peak in 510～550nm limit of visible spectrum, absorbing wavelength increases with the golden nanometer particle diameter.When particle diameter from small to large the time, apparent colour presents greenish orange yellow, vinicolor, peony and bluish violet successively to be changed.
The golden nanometer particle surface is atomic arrangement, because the existence of the crystal defects such as a large amount of twins, dislocation, fault, a large amount of outstanding key and unsaturated bond have been caused, the surface area of particle and surface-active are counted out significantly to be increased, have undersaturated character, many active centres occur, have very high chemism, easily combine and tend towards stability with other atoms, so exist the problem of easy reunion between golden nanometer particle.The reunion of golden nanometer particle further has influence on the size of golden nanometer particle, yet the properties such as the optics of golden nanometer particle, electricity, catalysis are subjected to the impact of particle size size to a great extent, and the problem that therefore solves the golden nanometer particle reunion has great importance for the performance that improves gold nano-material.
In order to solve the agglomeration traits of golden nanometer particle, the synthetic of golden nanometer particle often carries out synchronously with finishing, at first reducing agent is reduced into gold atom with the compound of gold, gold atom is further assembled the formation golden nanometer particle, when forming golden nanometer particle, add the adsorption stabilizing agent to increase stability, perhaps the decorating molecule direct-assembling forms the golden nanometer particle that contains self assembled monolayer to its surface.Employed reducing agent has natrium citricum, sodium borohydride, ascorbic acid, white phosphorus, ethanol or polyol etc., and employed stabilizing agent has natrium citricum, polymer, organic ligand etc.
The classical way of synthetic golden nanometer particle is the natrium citricum reducing process, so far still be widely used, utilize the method to prepare in the process of golden nanometer particle, natrium citricum not only as reducing agent also as stabilizing agent, can control the size of particle by the mol ratio that changes natrium citricum and tetra chlorauric acid, but the increase along with size, the polydispersity of particle also increases thereupon, particle morphology shows as ellipsoid or other is irregularly shaped, particle stability reduces, especially in synthesizing in batches, be difficult to the repeatability between the control different batches.
Phase transfer method also is that golden nanometer particle prepares one of common method, need to use the organic solvent extracting in the preparation process, make organosol, then through dewatering, take off organic solvent, namely make gold nano-material, the golden nanometer particle that the method makes is even, good dispersion, but operating process is complicated, organic solvent consumes morely in the preparation process, has greater environmental impacts.
Summary of the invention
The invention provides the method that a kind of catalase reduction tetra chlorauric acid prepares golden nanometer particle, the method has solved the easily reunion of conventional method golden nanometer particle, poor repeatability, complex process, problem that the preparation agents useful for same is large to ambient influnence.
A kind of catalase reduction tetra chlorauric acid prepares the method for golden nanometer particle, may further comprise the steps: the hydrogen peroxide enzyme solutions is added drop-wise in the tetra chlorauric acid solution, stirs; The pH value of regulating mixed solution is alkalescence, and reacts under 20～37 ℃ water bath condition, after reaction is finished, separates obtaining golden nanometer particle.
Catalase (Catalase) is as a kind of protein molecule, and wide material sources are cheap, have good biocompatibility, in the present invention as the template of synthetic golden nanometer particle.Contain a large amount of amino, sulfydryl binding site in the catalase molecule, can be used for fixedly tetra chlorauric acid, growth provides good space structure to golden nanometer particle, can effectively stop golden nanometer particle to be assembled in building-up process; In addition, the tyrosine in the catalase and the lysine, the arginine that contain primary amino radical have reproducibility under alkali condition, but the in-situ reducing tetra chlorauric acid.
Described catalase is preferably beef liver catalase.
Described catalase solution concentration is preferably 5～50mg/mL, and more preferably 5～20mg/mL most preferably is 5mg/mL, and the hydrogen peroxide enzyme dosage is fewer, and preparation cost is lower.
When the tetra chlorauric acid solution concentration is too high, can cause the reunion of golden nanometer particle, finally make the hydraulic performance decline of golden nanometer particle, the concentration of described tetra chlorauric acid solution is preferably 0.1%～1%, and more preferably 0.5%～1%, most preferably be 1%.
Single catalase molecule binding site is limited, therefore the mol ratio between catalase and the tetra chlorauric acid can affect size and the output of golden nanometer particle, mol ratio between catalase and the tetra chlorauric acid is higher, the reproducibility of mixed solution is stronger, the catalase molecule binding site that provides is more, then the amount of the golden nanometer particle of combination is fewer on the Single locus, thereby the golden nanometer particle size that forms is less, therefore by regulating the mol ratio of catalase and tetra chlorauric acid, can obtain the controlled golden nanometer particle of size, the mol ratio of catalase and tetra chlorauric acid is preferably 0.00221: 1～and 0.221: 1, in this molar ratio range, can obtain the golden nanometer particle that particle diameter is 10～40nm, the particle size range of golden nanometer particle is narrower, and the golden nanometer particle that obtains has preferably performance.
The mol ratio of catalase and tetra chlorauric acid more preferably 0.00221: 1～0.0885: 1 in this molar ratio range, can obtain the golden nanometer particle that particle diameter is 10～30nm, and higher mole ratio causes the golden nanometer particle character with enzyme more.
Under alkali condition, be conducive to improve the reproducibility of catalase reproducibility group, the pH value of described mixed solution is preferably 10～12; More preferably 12.
Described reaction finish refer to react do not change to the ultraviolet spectra curve of the golden nanometer particle colloidal sol that generates till, illustrate that the output of golden nanometer particle reaches stable state this moment.
The method of described separation is preferably centrifugal, and the rotating speed when centrifugal is preferably 5000～10000r/min, and the method is simple to operate, can realize the quick separation of golden nanometer particle, and is less on the product impact simultaneously.
Compared with prior art, beneficial effect of the present invention is:
(1) the present invention has introduced catalase as reducing agent and protective agent; reproducibility functional group on catalase reproducibility under alkali condition is strong; be conducive to the synthetic of golden nanometer particle, the golden nanometer particle of generation (0.5MNaCl) under high salt condition can not reunited.
(2) catalase is cheap among the present invention, and the cost of preparation golden nanometer particle is low.
(3) simple operating steps of the present invention, good reproducibility, reaction speed is fast, and reaction condition is gentle, the agents useful for same environmental friendliness, the golden nanometer particle of preparation has good biocompatibility.
Description of drawings
Fig. 1 is the golden nanometer particle transmission electron microscope picture that the embodiment of the invention 1 makes, and scale is 10nm.
Fig. 2 is that the golden nanometer particle that makes of the embodiment of the invention 1 adds the contrast figure before and after the 0.5MNaCl.
Fig. 3 is the golden nanometer particle transmission electron microscope picture that the embodiment of the invention 2 makes, and scale is 20nm.
Fig. 4 is the golden nanometer particle transmission electron microscope picture that the embodiment of the invention 3 makes, and scale is 50nm.
The visible light spectrogram of Fig. 5 golden nanometer particle that to be the present invention make under different catalases/tetra chlorauric acid mol ratio (0.00221: 1,0.0221: 1,0.0885: 1) condition.
The specific embodiment
(1) under the magnetic agitation condition, be that to be added drop-wise to concentration be in 1% the tetra chlorauric acid aqueous solution for beef liver catalase (the Aladdin reagent Co., Ltd) aqueous solution of 5mg/mL with concentration, until the mol ratio of catalase and tetra chlorauric acid is 0.00221: 1, magnetic agitation 4h;
(2) in (1) middle gained solution, add the 1mol/L sodium hydroxide solution, regulator solution pH to 10, stirred in water bath at 25 ℃, till extremely the golden nanometer particle colloidal sol ultraviolet spectra curve of reaction generation does not change, with gained golden nanometer particle colloidal sol under the rotating speed of 8000r/min, after the centrifugation, namely get golden nanometer particle.
As can be seen from Figure 1, resulting materials is the golden nanometer particle of particle diameter between 10-20nm; Fig. 2 shows that the golden nanometer particle (0.5MNaCl) under high salt condition of generation can not reunite.
(1) under the magnetic agitation condition, with concentration be the beef liver catalase aqueous solution of 5mg/mL to be added drop-wise to concentration be in 0.1% the tetra chlorauric acid aqueous solution, until the mol ratio of catalase and tetra chlorauric acid is 0.0221: 1, magnetic agitation 2h;
(2) to 1) in add the 1mol/L sodium hydroxide solution in the gained solution, regulator solution pH to 11, stirred in water bath at 30 ℃, till extremely reaction generation golden nanometer particle colloidal sol ultraviolet spectra curve does not change, with gained golden nanometer particle colloidal sol under the rotating speed of 8000r/min, after the centrifugation, namely get golden nanometer particle.
As can be seen from Figure 3, resulting materials is the golden nanometer particle of particle diameter between 10-40nm.
Embodiment 3
(1) under the magnetic agitation condition, with concentration be the beef liver catalase aqueous solution of 20mg/mL to be added drop-wise to concentration be in 0.1% the tetra chlorauric acid aqueous solution, until the mol ratio of catalase and tetra chlorauric acid is 0.0885: 1, magnetic agitation 60min;
(2) in (1) middle gained solution, add the 2mol/L sodium hydroxide solution, regulator solution pH to 10, stirred in water bath at 35 ℃, till extremely reaction generation golden nanometer particle colloidal sol ultraviolet spectra curve does not change, with gained golden nanometer particle colloidal sol under the rotating speed of 8000r/min, after the centrifugation, namely get golden nanometer particle.
As can be seen from Figure 4, resulting materials is the golden nanometer particle of particle diameter between 10-40nm.
Among Fig. 5, article three, curve is distinguished corresponding embodiment 1～3, it is the visible spectrum absorption curve of the golden nanometer particle that makes under different catalases/tetra chlorauric acid mol ratio condition, article three, the absworption peak of spectral absorption curve is consistent with the plasma absorption peak of golden nanometer particle feature, proved that catalase is to effective reduction of tetra chlorauric acid and the generation of golden nanometer particle, and when hydrogen peroxide enzyme and tetra chlorauric acid mol ratio are 0.0221: 1, the absorption value that visible spectrum curve absworption peak is corresponding is maximum, illustrates that the golden nanometer particle concentration that generates under this mol ratio condition is the highest.
Claims (9)
1. catalase reduction tetra chlorauric acid prepares the method for golden nanometer particle, may further comprise the steps:
The hydrogen peroxide enzyme solutions is added drop-wise in the tetra chlorauric acid solution, stirs; The pH value of regulating mixed solution is alkalescence, and reacts under 20～37 ℃ water bath condition, after reaction is finished, separates obtaining golden nanometer particle.
2. the method for claim 1 is characterized in that, described catalase is beef liver catalase.
3. the method for claim 1 is characterized in that, described catalase solution concentration is 5～50mg/mL.
4. method as claimed in claim 3 is characterized in that, described catalase solution concentration is 5～20mg/mL.
5. the method for claim 1 is characterized in that, the concentration of described tetra chlorauric acid solution is 0.1%～1%.
6. the method for claim 1 is characterized in that, the mol ratio of catalase and tetra chlorauric acid is 0.00221: 1～0.221: 1.
7. method as claimed in claim 6 is characterized in that, the mol ratio of catalase and tetra chlorauric acid is 0.00221: 1～0.0885: 1.
8. the method for claim 1 is characterized in that, the pH value of described mixed solution is 10～12.
9. method according to claim 8 is characterized in that, the pH value of described mixed solution is 12.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103940765A (en) * | 2014-04-25 | 2014-07-23 | 厦门大学 | Bio-functionalized nanometer microsphere particle combined chloroauric acid-gold nanoparticle probe as well as preparation method and application thereof |
| CN103978225A (en) * | 2014-05-14 | 2014-08-13 | 广西师范大学 | Method for quickly preparing stable nano-gold sol |
| CN104275492A (en) * | 2013-07-02 | 2015-01-14 | 济南大学 | Sugarcoated-haws-on-stick shaped one-dimensional gold nano-particle preparation method |
| CN104275493A (en) * | 2013-07-02 | 2015-01-14 | 济南大学 | Method for preparing gold nanoplates with amino acid as reducing agent |
| CN105823775A (en) * | 2016-03-22 | 2016-08-03 | 中国石油大学(华东) | Kit for detecting residual thiophosphate organophosphorus pesticides and application method of kit |
| CN111975010A (en) * | 2019-05-21 | 2020-11-24 | 中国药科大学 | Preparation of gold nanoparticles based on D-arabinose reduction |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104275492A (en) * | 2013-07-02 | 2015-01-14 | 济南大学 | Sugarcoated-haws-on-stick shaped one-dimensional gold nano-particle preparation method |
| CN104275493A (en) * | 2013-07-02 | 2015-01-14 | 济南大学 | Method for preparing gold nanoplates with amino acid as reducing agent |
| CN104275492B (en) * | 2013-07-02 | 2016-06-22 | 济南大学 | A kind of preparation method of the one-dimensional golden nanometer particle of sugarcoated haws on a stick shape |
| CN104275493B (en) * | 2013-07-02 | 2016-07-06 | 济南大学 | A kind of method preparing gold nano dish for reducing agent with aminoacid |
| CN103940765A (en) * | 2014-04-25 | 2014-07-23 | 厦门大学 | Bio-functionalized nanometer microsphere particle combined chloroauric acid-gold nanoparticle probe as well as preparation method and application thereof |
| CN103978225A (en) * | 2014-05-14 | 2014-08-13 | 广西师范大学 | Method for quickly preparing stable nano-gold sol |
| CN105823775A (en) * | 2016-03-22 | 2016-08-03 | 中国石油大学(华东) | Kit for detecting residual thiophosphate organophosphorus pesticides and application method of kit |
| CN111975010A (en) * | 2019-05-21 | 2020-11-24 | 中国药科大学 | Preparation of gold nanoparticles based on D-arabinose reduction |
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Effective date of registration: 20200831 Address after: 710000 Shaanxi Xi'an economic and Technological Development Zone, Fengcheng four road, Ming Guang road, southeast corner of the new business building 1808 Patentee after: SHAANXI ZHUANYI INTELLECTUAL PROPERTY OPERATION Co.,Ltd. Address before: Hangzhou City, Zhejiang province 310018 Xiasha Higher Education Park No. 2 Street No. 5 Patentee before: Zhejiang University of Technology |
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