CN102841033A - Fresh tobacco leaf moisture content determination method - Google Patents
Fresh tobacco leaf moisture content determination method Download PDFInfo
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- CN102841033A CN102841033A CN2012103527455A CN201210352745A CN102841033A CN 102841033 A CN102841033 A CN 102841033A CN 2012103527455 A CN2012103527455 A CN 2012103527455A CN 201210352745 A CN201210352745 A CN 201210352745A CN 102841033 A CN102841033 A CN 102841033A
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- tobacco leaf
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- water percentage
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Abstract
The invention discloses a fresh tobacco leaf moisture content determination method. After weighing a fresh tobacco leaf sample, the method uses liquid nitrogen to quick-freeze the fresh tobacco leaf sample, and stores the fresh tobacco leaf sample in a refrigerator; during determination, the tobacco leaf sample is taken out of the refrigerator, a freeze drier is adopted to remove moisture, the tobacco leaf sample is then weighed, and therefore the fresh tobacco leaf moisture content is worked out. The specific method includes: (1) sampling and weighing of fresh tobacco leaves; (2) low-temperature storage after liquid nitrogen freezing; (3) freeze drying by the freeze drier; (4) fresh tobacco leaf moisture content calculation. The determination result of the method provided by the invention is accurate, so that the defect of the prior art is overcome; moreover, the processed tobacco leaf sample is as brightly green as the fresh tobacco leaves; and meanwhile, the polyphenol substance content and aromatic ingredient content of tobacco leaves are kept to the max. The method can help to know the quality state of the tobacco leaves. The method is applicable to the scientific research and testing of the tobacco leaves.
Description
Technical field
The present invention relates to tobacco, specifically, relate to the assay method of tobacco leaf water percentage.
Background technology
Flue-cured tobacco be a kind of with results plant leaf be the crop of purpose; The height of its leaf water content is closely related with its quality; Therefore the content of moisture is used as the important indicator of a quality of tobacco in the fresh tobacco leaf, and traditional assay method adopts the baking oven oven drying method that completes, and is about to carry out 105 ℃ after fresh tobacco leaf will be weighed completed 2 hours; Dry to constant weight for 70 ℃, calculate the water percentage of plant through the minimizing of measuring oven dry back weight.This method shortcoming is to be accompanied by the high temperature that completes with drying course, and the conversion and the degraded of material have taken place in the tobacco leaf, the minimizing of volatilizing gradually of some volatile matter.Cause the final water percentage result who measures more higher than actual water percentage in the fresh tobacco leaf.
The patented claim part that relates to moisture content in leaves in the Chinese patent database only has " a kind of method for dehydrating tobaccos and specialized equipment thereof " No. 200910064256.8; This method is water cut to be put in the device above the tobacco leaf of depositing standard send in the vacuum dewatering apparatus, handles the tobacco leaf of Moisture high UCL.The water percentage that does not relate to tobacco leaf.Up to now, also do not see the application part of the mensuration of tobacco leaf water percentage.
Summary of the invention
The object of the present invention is to provide a kind of assay method of fresh tobacco leaf water percentage, to overcome the inaccurate defective of traditional assay method result.
The assay method of the fresh tobacco leaf water percentage that the inventor provides is with liquid nitrogen flash freezer behind the fresh tobacco leaf samples weighing; Place-80 ℃ of refrigerators to preserve; During mensuration tobacco sample taken out from refrigerator and weigh after adopting freeze drier to remove moisture, thereby calculate the fresh tobacco leaf water percentage; Specific practice is:
(1) the fresh tobacco leaf sampling is weighed
Round sheet fresh tobacco leaf sample from the flue-cured tobacco plant of normal growth, weigh, be designated as W
1
(2) cryopreservation behind the liquid nitrogen frozen
Put into liquid nitrogen snap frozen 5min after tobacco sample is weighed immediately, then put into refrigerator immediately, in-80 ℃ of following cryopreservation;
(3) freeze drier freeze drying
Treat to put into freeze drier behind the fresh tobacco leaf sample drying, keep 5h down, the phase one distillation in-20 ℃; Dry 15h under 0 ℃, the subordinate phase distillation; Dry under 20 ℃ afterwards, until the sample constant weight, be designated as W
2
(4) the fresh tobacco leaf water percentage calculates
The fresh tobacco leaf water percentage is calculated as follows:
Water percentage=(W
1-W
2)/W
1* 100.
The inventor points out: compare with traditional fresh tobacco leaf measurement of water-content coefficient method, this method is that the fresh tobacco leaf sample is refrigerated to the below freezing of water, under vacuum, makes moisture wherein be directly sublimed into gaseous state without liquid state then, and left a kind of in sample of material itself
MeasureMethod.Because sample is in freezing state in the whole mensuration process, the effect of microbial growth and enzyme can't be carried out, and some the volatile ingredient losses in the sample are very little; Volume is almost constant; Kept original structure, concentration phenomena can not take place, and drying can be got rid of the moisture content more than 99%; Make the dry back product can long preservation and unlikely rotten, reach and measure the result comparatively accurately.
It is accurate that method provided by the invention is not only measured the result, overcome the defective of original technology; And tobacco sample is emerald green after handling, with the fresh tobacco leaf solid colour; The polyphenol substance content and the fragrance component content that have kept simultaneously tobacco leaf to greatest extent.Help grasping the quality situation of fresh tobacco leaf.Be applicable to scientific research and the detection of tobacco.
Embodiment
Following examples are used to further specify the present invention:
Embodiment chooses and transplants back 70 days whole strain tobacco leaves for supplying the examination object, removes the pin leaf, and the employing half leaf method is divided into two its tobacco leaf and removes master pulse, and half is put into baking oven and adopts traditional oven drying method that completes, and second half adopts the inventive method to remove moisture.
Take complete half tobacco leaf of oven drying method of routine, samples weighing is W
1=465.75g, 105 ℃ of 2h that complete, 70 ℃ of oven dry 48h are weighed as W to constant weight
2=75.42g is through calculating back fresh tobacco leaf water percentage=(W
1-W
2)/W
1* 100=(465.75-75.42)/465.75 * 100=83.80%.
Take of the present invention half thely, tobacco sample is weighed as W
1=442.58g; Put into liquid nitrogen snap frozen 5min after weighing immediately, then put into refrigerator immediately, in-80 ℃ of preservations; Treat to put into freeze drier behind the fresh tobacco leaf sample drying, keep 5h, the phase one distillation in-20 ℃; Dry 15h under 0 ℃, the subordinate phase distillation; Dry under 20 ℃ afterwards, be W until the sample constant weight
2=91.04g; Through calculating back fresh tobacco leaf water percentage=(W
1-W
2)/W
1* 100=(442.58-91.04)/442.58 * 100=79.43%.
The result
1. this method and complete oven drying method tobacco sample color and weight, volume ratio are
It is widely different that the oven drying method that completes and the inventive method are handled color, volume and the dry matter content of back sample, and the complete sample of oven drying method of tobacco leaf color is the bluish yellow look; And the tobacco leaf of this method is emerald green, with the fresh tobacco leaf solid colour; After the fresh tobacco leaf of identical weight was handled, the fresh tobacco leaf moisture dry tobacco leaf of method that completes was 83.80% (dry matter content is 16.20%), and this method is 79.43% (dry matter content is 20.57%), and the two water percentage differs 4.37%; On the volume, volume is to complete 3.25 times that dry after fresh tobacco leaf this method drying of identical weight.
2. the polyphenol substance content of this method and the oven drying method processing tobacco leaf that completes relatively
The polyphenol substance content of tobacco leaf was as shown in table 1 after two kinds of methods were handled:
Table 1 distinct methods is handled back tobacco leaf polyphenol substance content relatively (mg/g)
Can find out by table 1; This method is very big with the sample polyphenols content difference that the oven drying method that completes is handled, and the neochlorogenic acid in the tobacco leaf of this method method drying, chlorogenic acid, 4-O-coffee Kui Buddhist nun alcohol, rutin sophorin and the luxuriant and rich with fragrance alcohol radical rutin sophorin of camphane content all are higher than the oven drying method that completes extremely significantly.Wherein the chlorogenic acid in the dry tobacco leaf of this method and the pure content of 4-O-coffee Kui Buddhist nun are in the tobacco leaf of oven drying method 4.44 times of completing; And neochlorogenic acid and with rutin sophorin be in the tobacco leaf of oven drying method 4.43 times and 3.88 times of completing; The luxuriant and rich with fragrance alcohol radical rutin sophorin of camphane content is 2.36 times.The traditional oven drying method material that completes of this explanation has bigger conversion and degraded.
3. fragrance component content in the tobacco leaf after the freeze drying
The fragrance component that contains jointly in this law and the dried tobacco leaf of oven drying method that completes only has 11 kinds; And contain in the dry tobacco leaf of this law but the fragrance component that do not contain in the method tobacco leaf that completes has 9 kinds, and contain in the dry tobacco leaf of the method that completes but the fragrance component that do not contain in the dry tobacco leaf of this law method up to 21 kinds.Explanation part volatile substances in the process of oven dry that completes is fallen, and some non-volatile matters such as chlorogenic acid have been converted into other materials such as pyrrole heavy stone used as an anchor, have influenced the mensuration result of water percentage.
Table 2 distinct methods is handled in the tobacco leaf of back and is detected fragrance component content (ng/g)
| Sequence number | The compound title | This method | Method completes |
| 1 | Furfural | 185.36±135.79 Aa | 234.16±64.18 Aa |
| 2 | Indoles | 18.51±0.86 Aa | 24.43±14.64 Aa |
| 3 | Benzaldehyde | 164.46±28.55 Aa | 12.87±2.04 Bb |
| 4 | Phenylethyl alcohol | 194.43±54.80 Aa | 13.52±1.90 Bb |
| 5 | Linalool | 8.46±5.57 Aa | 8.71±3.67 Aa |
| 6 | The eggplant diketone falls | 82.90±24.01 Aa | 121.37±109.25 Aa |
| 7 | Damascenone | 293.36±15.34 Aa | 213.00±94.87 Aa |
| 8 | Alpha, beta-lonone | 0.79±0.37 Ab | 2.43±1.01 Aa |
| 9 | Farnesyl acetone | 26.08±3.33 Aa | 2.32±1.88 Bb |
| 10 | Neophytadiene | 1955.03±166.38 Aa | 1326.02±608.99 Aa |
| 11 | The 2-pentenals | 486.59±31.12 Aa | 1.26±0.12 Bb |
| 12 | The 3-methyl butanol | 64.17±13.71 | no |
| 13 | The 2-pentenol | 32.46±1.45 | no |
| 14 | 3-methyl-2-butene alcohol | 32.93±7.16 | no |
| 15 | The 3-pentenals | 20.00±9.85 | no |
| 16 | 4-alkene guaiacol | 27.52±1.84 | no |
| 17 | Phenol | 540.31±174.08 | no |
| 18 | Benzophenone | 409.82±51.15 | no |
| 19 | Hexanal | 24.68±1.95 | no |
| 20 | Aldehyde C-9 | 4.78±0.11 | no |
| 21 | 2-methyl-3 (2H)-furanone | no | 3.24±1.19 |
| 22 | 2-methyl-pyrazine | no | 8.54±2.39 |
| 23 | The 2-acetyl pyrrole | no | 18.42±19.45 |
| 24 | The 2-acetyl furan | no | 15.17±3.76 |
| 25 | 5,6-dimethyl-2-benzo imidazolidinone | no | 61.15±61.97 |
| 26 | 5 methyl furfural | no | 27.23±7.75 |
| 27 | 6-methyl-3,5-two hepten-2-ones | no | 3.55±2.32 |
| 28 | The pyrrole heavy stone used as an anchor | no | 6.76±3.54 |
| 29 | Furfuryl alcohol | no | 46.18±38.60 |
| 30 | Phenylacetaldehyde | no | 321.76±75.76 |
| 31 | Solanone | no | 413.72±205.8 |
| 32 | Dihydroactinidiolide | no | 46.33±43.49 |
| 33 | Megastigmatrienone B | no | 10.98±8.70 |
| 34 | Megastigmatrienone C | no | 9.43±7.56 |
| 35 | Geranyl acetone | no | 15.52±4.36 |
| 36 | The oxidation isophorone | no | 2.16±2.45 |
| 37 | Isophorone | no | 2.24±2.17 |
| 38 | Safranal | no | 6.85±5.20 |
| 39 | The dihydro safranal | no | 5.92±4.37 |
| 40 | The 2-methyl butanol | no | 1.04±0.08 |
| 41 | 1,3, the 5-heptenal | no | 14.2±2.26 |
Annotate: no representes not detect fragrance component.
Claims (1)
1. the assay method of a fresh tobacco leaf water percentage; It is characterized in that liquid nitrogen flash freezer behind the fresh tobacco leaf samples weighing; Place-80 ℃ of refrigerators to preserve, during mensuration tobacco sample taken out from refrigerator and weigh after adopting freeze drier to remove moisture, thus calculating fresh tobacco leaf water percentage; Specific practice is:
(1) the fresh tobacco leaf sampling is weighed
Round sheet fresh tobacco leaf sample from the flue-cured tobacco plant of normal growth, weigh, be designated as W
1
(2) cryopreservation behind the liquid nitrogen frozen
Put into liquid nitrogen snap frozen 5min after tobacco sample is weighed immediately, then put into refrigerator immediately, in-80 ℃ of following cryopreservation;
(3) freeze drier freeze drying
Treat to put into freeze drier behind the fresh tobacco leaf sample drying, keep 5h down, the phase one distillation in-20 ℃; Dry 15h under 0 ℃, the subordinate phase distillation; Dry under 20 ℃ afterwards, until the sample constant weight, be designated as W
2
(4) the fresh tobacco leaf water percentage calculates
The fresh tobacco leaf water percentage is calculated as follows:
Water percentage=(W
1-W
2)/W
1* 100.
Priority Applications (1)
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|---|---|---|---|
| CN2012103527455A CN102841033A (en) | 2012-09-21 | 2012-09-21 | Fresh tobacco leaf moisture content determination method |
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|---|---|---|---|
| CN2012103527455A CN102841033A (en) | 2012-09-21 | 2012-09-21 | Fresh tobacco leaf moisture content determination method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103604721A (en) * | 2013-11-21 | 2014-02-26 | 甘肃农业大学 | Determination method for mass of plant anther and pollen |
| CN103743646A (en) * | 2014-01-13 | 2014-04-23 | 广东中烟工业有限责任公司 | Measurement method for content of moisture in tobacco and tobacco product |
| CN104596885A (en) * | 2015-02-09 | 2015-05-06 | 河南科技学院 | Method for determining content of total solid matters of yellow seriflux of bean products |
| CN106769631A (en) * | 2017-01-16 | 2017-05-31 | 云南中烟再造烟叶有限责任公司 | The rapid assay methods of moisture content in a kind of papermaking-method reconstituted tobaccos production |
| CN107655785A (en) * | 2017-09-28 | 2018-02-02 | 贵州省烟草公司贵阳市公司 | A kind of method of the first flue-cured tobacco water content detection rate of raising |
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| US4392501A (en) * | 1981-07-06 | 1983-07-12 | Brown & Williamson Tobacco Corporation | Method of processing fresh tobacco leaves |
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| KR20050055483A (en) * | 2003-12-08 | 2005-06-13 | 주식회사 케이티앤지 | Process for the expansion of tobacco |
| CN101317562A (en) * | 2008-07-17 | 2008-12-10 | 吕坤秋 | Biological vacuum freezing and drying method for aweto |
| CN101393099A (en) * | 2008-10-30 | 2009-03-25 | 中国烟草总公司郑州烟草研究院 | Continuous measurement method for tobacco moisture percentage and apparatus thereof |
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| US4392501A (en) * | 1981-07-06 | 1983-07-12 | Brown & Williamson Tobacco Corporation | Method of processing fresh tobacco leaves |
| US5235992A (en) * | 1991-06-28 | 1993-08-17 | R. J. Reynolds Tobacco Company | Processes for producing flavor substances from tobacco and smoking articles made therewith |
| KR20050055483A (en) * | 2003-12-08 | 2005-06-13 | 주식회사 케이티앤지 | Process for the expansion of tobacco |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103604721A (en) * | 2013-11-21 | 2014-02-26 | 甘肃农业大学 | Determination method for mass of plant anther and pollen |
| CN103604721B (en) * | 2013-11-21 | 2015-09-23 | 甘肃农业大学 | The assay method of plant anther and pollen quality |
| CN103743646A (en) * | 2014-01-13 | 2014-04-23 | 广东中烟工业有限责任公司 | Measurement method for content of moisture in tobacco and tobacco product |
| CN103743646B (en) * | 2014-01-13 | 2016-08-17 | 广东中烟工业有限责任公司 | One grows tobacco and the assay method of tobacco product moisture content |
| CN104596885A (en) * | 2015-02-09 | 2015-05-06 | 河南科技学院 | Method for determining content of total solid matters of yellow seriflux of bean products |
| CN106769631A (en) * | 2017-01-16 | 2017-05-31 | 云南中烟再造烟叶有限责任公司 | The rapid assay methods of moisture content in a kind of papermaking-method reconstituted tobaccos production |
| CN107655785A (en) * | 2017-09-28 | 2018-02-02 | 贵州省烟草公司贵阳市公司 | A kind of method of the first flue-cured tobacco water content detection rate of raising |
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Application publication date: 20121226 |