CN102838589B - Method for preparing N substituted pyrrolidine derivative through acylation of methyl sulfone chloride - Google Patents
Method for preparing N substituted pyrrolidine derivative through acylation of methyl sulfone chloride Download PDFInfo
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Abstract
The invention relates to an N-substituted pyrrolidine derivative with a structure shown in a formula (I) and a curative active substance of the N-substituted pyrrolidine derivative serving as a DPP-IV inhibitor for treating and/or preventing diabetes mellitus or other diseases relative to DPP-IV.
Description
The application is to be September 23, application number in 2008 be CN 200810161373.1 the dividing an application of Chinese invention patent application " pyrrolidin derivatives that N-replaces and uses thereof " applying date.
Technical field
The present invention relates to the pyrrolidin derivatives that a kind of N-replaces, belong to pharmaceutical field.
Background technology
Diabetes are a kind of lysis being caused by multiple paathogenic factor, at fasting state or oral glucose tolerance test period, take after glucose, show as hyperglycemia or plasma glucose levels and raise.Diabetes have two kinds of common form, I type and type ii diabetes.Type i diabetes (or claiming insulin-dependent diabetes mellitus (IDDM)) patient seldom produces or does not produce completely Regular Insulin; Type ii diabetes (or claiming non insulin dependent diabetes (NIDDM)) patient compares with ND, patient's plasma insulin level is often identical even higher, these patients' main insulin sensitivity tissue, muscle, liver and fatty tissue etc. have produced resistance to the hormesis of Regular Insulin, and Regular Insulin is difficult to bring into play the effect of its normal regulating and controlling blood sugar balance.
Current hypoglycemic method and medicine are more, and the minimizing of physical activity and meals calorie intake may improve the symptom of diabetes, but acts on limited; Give with the sulfonylurea that can stimulate the more Regular Insulin of beta cell secretion (for example, tolbutamide and Glipizide) or meglitinide, and/or at sulfonylurea or meglitinide when invalid, by insulin injection, increase the insulin level of blood plasma, to stimulate the tissue that Regular Insulin has been produced to resistance, but the secretogogue that directly gives Regular Insulin or Regular Insulin has and causes hypoglycemic risk, may there is the high-caliber insulin resistance that blood plasma hyperinsulinism concentration causes simultaneously.Biguanides can improve insulin sensitivity, thereby hyperglycemia is relaxed to some extent, but the biguanides such as phenformin and N1,N1-Dimethylbiguanide all can bring out lactic acidosis, feel sick or diarrhoea.Glitazones(, 5-benzyl thiazolidine-2,4-diketone) be effective compound that improves the multiple symptom of type ii diabetes that a class is new, in the model of the animal of several type ii diabetes, these medicines have improved the insulin sensitivity in muscle, liver and fatty tissue substantially, thus can part reduction blood plasma high glucose level.
DPP IV (DPP-IV) be a kind of from H-Xaa-Pro(wherein Xaa be that amino acid, preferred a kind of lipophilic amino acid and Pro are proline(Pro) arbitrarily) serine protease of cracking N--end dipeptides the peptide class that starts.It also can be used as the peptide substrate class starting with sequence H-Xaa-Ala (wherein Ala is L-Ala).DPP-IV is extensively present in epithelial lining, the mesoblastema in some tissue and the specific t lymphocyte subset group surface of various tissues, maximum with renal cortex content, is secondly lung, suprarenal gland, jejunum, liver etc., with cross-film and soluble form, expresses.It belongs to a member of prolyl oligopeptidase family, is the high specific serine protease existing with dimeric forms, and each subunit comprises two structural domains, i.e. a α/β lytic enzyme structural domain and octahedral β-spirane structure territory; Between these two structural domains, having the approximately large-scale cave of 30～45A of a size, is the gangway of controlling substrate, has a bag shaped structure in it, is the reactive site of DPP-IV.The substrate of DPP-IV is the protein that has proline(Pro) (Pro) or L-Ala (Ala) in N-terminal penultimate, can, from two amino-acid residues of N-terminal hydrolysis of peptide chain, make many biologically active peptides inactivations.
DPP-IV has the effect in activated T lymphocytes, and DPP-IV is identical with T cell protein CD26, and DPP-IV inhibitor can regulate t cell responses, can be developed as new immunomodulator; In addition, because CD26 is the necessary coreceptor of HIV, so DPP-IV inhibitor likely can be in order to treat AIDS.
DPP-IV also have the effect of immunity system outside: DPP-IV can increase glucagon-like-peptide-1 and-2(GLP-1 and GLP-2) etc. the degraded of several peptide hormones.Glucose plays keying action aspect glucose balance according to patience pancreotropic hormone polypeptide (GIP) and glucagon-like-peptide-1 (GLP-1) in maintaining body, it can stimulate human body to produce the situation that Regular Insulin raises with reply glucose level, this external enwergy reduces the glucose being produced by liver, slow down food absorption speed, produce the sensation of satiety and reduce appetite.The effect of type ii diabetes patient's GIP insulin secretion accelerating is obstructed, and only has GLP-1 can bring into play the effect of insulin secretion accelerating, thereby and suppresses the sensation that stomach emptying produces satiety and reduce appetite.So by suppressing DPP-IV, thereby indirectly increase the effect that the level of blood plasma glucagon-like-peptide-1 can produce the diseases such as treatment type ii diabetes and impaired glucose tolerance.
At present, document discloses the inhibitor of part DPP-IV, although found some lead compounds from random screening program, the major part work in this field concentrates on studies substrate analogue.In the research of substrate analogue, find; the most effective inhibitor is aminoacyl tetramethyleneimine boric acid class, but they are unsettled, trend towards cyclisation; the tetramethyleneimine that some other is more stable and tetrahydrothiazole derivates are lower to the affinity of enzyme, need heavy dose in clinical scenarios.Cyanopyrrole alkanes provides good compromise proposal, and they both had higher affinity to enzyme, has again the transformation period that length is moderate in the solution of free alkali.Vildagliptin/Vildaglitin (following formula A) (US6166063) and saxagliptin(following formula B) (WO2004052850, June 4 2004 International Publication day), the former got permission listing as the representative of Cyanopyrolidine derivatives.
Also have in addition a plurality of Cyanopyrolidine derivatives before clinical and in development of clinical studies process.(S)-1-ethanoyl-2-cyanopyrrole is the common drug activity groups of these compounds.Jens-Uwe Peters to Cyanopyrolidine research work during the last ten years made a detailed review (Current Topics in Medicinal Chemistry, 2007,7,579-595), therefrom can find still have many needs of work to carry out to the further research of this compounds.
Summary of the invention
Technical scheme of the present invention has been to provide a series of novel cpds that belong to the inhibitor of enzyme DPP-IV and be used for the treatment of some human body diseases.Another technical scheme of the present invention has been to provide the pharmaceutical composition that contains this compound.
The compounds of this invention is as described below by general formula I:
Wherein:
X is carbonyl, methylene radical or covalent linkage;
N is 0,1,2,3;
R is selected from C
1-C
8the give repeated exhortations various aromatic nucleus of base, isoquinolyl, indyl, pseudoindoyl and benzo-fused analogue thereof of alkyl, phenyl, pyridyl, pyridazinyl, pyrimidyl, pyrazinyl, rice azoles base, pyrazolyl, thiazolyl, isothiazolyl, oxazolyl, isoxazolyl, quinoline, they all can at random on one or more carbon atoms, be substituted and substituting group is chosen from C
1-C
8alkyl, hydroxyl, C
1-C
8alkoxyl group, amino, C
1-C
8alkylamino, C
1-C
8amide group, halogen, trifluoromethyl, nitro, cyano group, carboxyl and C
1-C
8alkoxy carbonyl.
The compound of general formula I is with basic functional group, and they can form salt with acid thus, and the salt forming with pharmaceutically acceptable acid is included in the scope of the present invention.Suitable sour example includes but not limited to acetic acid, oxalic acid, trifluoroacetic acid, citric acid, fumaric acid, phenylformic acid, pamoic acid, methylsulfonic acid, nitric acid, sulfuric acid, phosphoric acid etc.
In preferred embodiment of the present invention, X is methylene radical or carbonyl.
In preferred embodiment of the present invention, n is 0,1, or 2.
In the present invention, preferred compound includes but not limited to:
(S)-1-(2-(2-(3-phenmethyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(1,1-dimethyl ethyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(1-methylethyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(4-aminomethyl phenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-phenyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-cyclohexyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt;
(S)-1-(2-(2-(3-normal-butyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(3-fluorophenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(3,4-methylene-dioxy phenmethyl)-2-oxo-1-imidazolidyl) kharophen) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(4-p-methoxy-phenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) kharophen) ethanoyl-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(4-methylbenzene methyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt;
(S)-1-(2-(2-(3-(4-fluorophenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(3,4-Dimethoxyphenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(3,5-Dimethoxyphenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(2-phenylethyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt.
Further preferably:
(S)-1-(2-(2-(3-phenyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(4-aminomethyl phenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate and/or its salt,
(S)-1-(2-(2-(3-(4-fluorophenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(2-phenylethyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(3,4-Dimethoxyphenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(3,5-Dimethoxyphenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-phenmethyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt.
Further preferably: (S)-1-(2-(2-(3-(3,4-Dimethoxyphenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt,
(S)-1-(2-(2-(3-(3,5-Dimethoxyphenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine and/or its salt.
The present invention also provides a kind of pharmaceutical composition that contains above-claimed cpd and pharmacy acceptable salt thereof simultaneously, and the preparation that is prepared into of these compounds, composition, these preparations are to be prepared according to a conventional method after auxiliary material available on adding medicament by the compounds of this invention or composition, and its formulation includes but not limited to: tablet, granule, capsule, oral liquid and pharmaceutically acceptable other formulation.This pharmaceutical composition and preparation thereof can directly or indirectly produce the disease of clinical beneficial effect in treatment by the inhibition of DPP-VI or CD26: direct effect comprises the lymphocytic activation of blocking-up T, and indirect action comprises by preventing that these hormones from degrading strengthens the activity of peptide hormone.The example of disease include but not limited to autoimmune disease and such as enteritis and the such inflammatory disease of rheumatoid arthritis, cause growth hormone deficiency of short and small stature, polycystic ovary syndrome, impaired glucose tolerance and type ii diabetes.Particularly preferably be described compound and composition in treatment grape candy tolerance the purposes in abnormal and type ii diabetes and be similarly a kind of by giving the method for these diseases of compounds for treating as above of significant quantity.
In order to find the inhibitor similar drug of the DPP-IV that activity is stronger, security is better, contriver is by a large amount of research experiments, find and synthesized the cyanopyrrole hydride compounds that a class is new as described in the present invention, this class Cyanopyrolidine compound structure is different from the disclosed compound of prior art, the more existing similar compound of the result for the treatment of of type II diabetes is had to obvious advantage, onset is faster, better efficacy, can select for patient provides a kind of better medication.
Compound of the present invention can be prepared by method as known in the art.
Method one
Take Boc-glycine and proline methyl ester as raw material; with DCC and HOBT condensation, can obtain the sweet dried meat methyl esters of Boc-II, II can obtain the sweet dried meat acid amides of Boc-III by the saturated methanol solution ammonia solution of ammonia; III is through the phosphorus oxychloride IV that dewaters to obtain, and IV can obtain intermediate V with trifluoroacetic acid Deprotection again.
Method two
Take ammonia VI as raw material and phosphinylidyne imidazoles or phosgene reaction generation isocyanic ester VII, and isocyanic ester VII reacts with diethanolamine and generates intermediate VIII, and VIII cyclization after halo makes intermediate compound I X, and IX generates compounds X with sweet dried meat dipeptidase derivant coupling again.
Method three
Aldehyde XI and reacting ethylenediamine generate monosubstituted quadrol XII with sodium borohydride reduction again; XII cyclisation under the effect of phosphinylidyne imidazoles generates substituted imidazole alkane ketone XIII; XIII generates XIV by methyl bromoacetate hydrocarbonylation; XIV reduces to obtain compounds X V, after XV methylsulfonyl, obtains X again with the coupling of sweet dried meat dipeptidase derivant.
Method four
Compounds X IV hydrolysis can obtain sour XVI, and XVI reacts with sulfur oxychloride and generates acyl chlorides, and acyl chlorides reacts with sweet dried meat dipeptidase derivant and generates target product X VII.
Embodiment
In lower embodiment, further explain these general methods, but the not restriction to content of the present invention.
Embodiment 1
(S)-1-(2-(2-(3-phenyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 1.
A 1,1-bis-(2-hydroxyethyl)-3-phenylurea
Triphosgene (30g) is dissolved in toluene, drip aniline (18.5g), finish and reflux 5 hours, underpressure distillation is removed after unnecessary phosgene, splashed in the dichloromethane solution of diethanolamine (24g), stirring is spent the night again, extremely clean with saturated brine washing, dry concentrated 1,1-bis-(2-hydroxyethyl)-3-phenylurea (36g).
B 1-bromotrifluoromethane-3-phenyl-2-imidazolidone
1,1-bis-(2-hydroxyethyl)-3-phenylurea (36g) is dissolved in methylene dichloride, at the cooling lower dropping phosphorus tribromide of ice bath (40.6g), finishes stirring at room 5 hours.Reaction solution regulates pH to be about 10 with saturated sodium bicarbonate solution, then stirring at room 36 hours.Separate organic phase, water dichloromethane extraction three times, merge organic phase, saturated brine washing three times, and anhydrous sodium sulfate drying, concentrating under reduced pressure obtains crude product.Silicagel column is separated, methylene dichloride: methyl alcohol (99:3) wash-out obtains sterling (10g).ESI-MS:269,271。
C (S)-1-(2-(2-(3-phenyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate
1-bromotrifluoromethane-3-phenmethyl-2-imidazolidone (2.7g), (S)-1-(2-glycyl)-2-Cyanopyrolidine trifluoroacetate (2.6g) are dissolved in to N; dinethylformamide; add potash solid (3.5g); be heated to 50 degree reaction 15 hours; reaction solution dilutes by ethyl acetate; saturated sodium bicarbonate solution, saturated brine respectively wash three times, anhydrous sodium sulfate drying, and concentrating under reduced pressure obtains crude product.Silicagel column is separated, trichloromethane: methyl alcohol (95:5) wash-out obtains (S)-1-(2-(2-(3-phenyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine (0.5g).Be dissolved in Virahol again, splash into equimolar oxalic acid aqueous isopropanol, filtration drying obtains white solid (0.4g).
1H-NMR?δ(DMSO-d
6):7.59(d,2H);7.33(t,2H);7.01(t,1H);4.82(dd,1H);4.05-3.93(m,2H);3.81(t,2H);3.62-3.59(m,1H);3.51(t,4H);3.44-3.40(m,1H);3.10(t,2H);2.21-2.16(m,2H);2.05-2.00(m,2H).ESI-MS:342.
Embodiment 2
(S)-1-(2-(2-(3-(1,1-dimethyl ethyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 2.
(S) preparation of-1-(2-(2-(3-(1,1-dimethyl ethyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate is prepared by ordinary method with reference to embodiment mono-.
1H-NMRδ(DMSO-d
6):4.77(dd,1H,);4.09-4.03(m,2H);3.61-3.57(m,1H);3.46-3.40(m,5H);3.29-3.26(m,4H);2.28-2.24(m,2H);2.14-2.09(m,2H);1.26(s,9H).ESI-MS:322.
Embodiment 3
(S)-1-(2-(2-(3-(4-p-methoxy-phenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 3.
(S) preparation of-1-(2-(2-(3-(4-p-methoxy-phenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate is prepared by ordinary method with reference to embodiment mono-.
1H-NMR?δ(DMSO-d
6):7.48(d,2H);6.92(d,2H);4.83(dd,1H);4.02-3.92(m,1H);3.77(t,2H);3.72(s,3H);3.61-3.58(m,1H);3.49-3.47(m,4H);3.46-3.42(m,1H);3.10(t,2H);2.18-2.16(m,2H);2.07-2.00(m,2H).ESI-MS:372.
Embodiment 4
(S)-1-(2-(2-(3-(1-methylethyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 4.
(S) preparation of-1-(2-(2-(3-(1-methylethyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate is prepared by ordinary method with reference to embodiment mono-.
1H-NMR?δ(DMSO-d
6):4.82(dd,1H);3.95-3.88(m,3H);3.59-3.56(m,1H);3.43-3.39(m,1H);3.32(t,2H);3.27(t,2H);3.22(t,2H);3.00(t,2H);2.18-2.16(m,2H);2.06-2.01(m,2H);1.04(d,6H).ESI-MS:308。
Embodiment 5
(S)-1-(2-(2-(3-(4-aminomethyl phenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 5.
(S) preparation of-1-(2-(2-(3-(4-aminomethyl phenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate is prepared by ordinary method with reference to embodiment mono-.
1H-NMR?δ(DMSO-d
6):7.45(d,2H);7.13(d,2H);4.82(dd,1H);4.03-3.94(m,2H);3.78(t,2H);3.61-3.58(m,1H);3.50-3.47(m,4H);3.44-3.40(m,1H);3.10(t,2H);2.25(s,3H);2.20-2.16(m,2H);2.07-2.00(m,2H).ESI-MS:356。
Embodiment 6
(S)-1-(2-(2-(3-cyclohexyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 6.
(S) preparation of-1-(2-(2-(3-cyclohexyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate is prepared by ordinary method with reference to embodiment mono-.
1H-NMRδ(DMSO-d
6):4.83(dd,1H);4.00-3.90(m,2H);3.60-3.57(m,1H);3.51-3.46(m,1H);3.43-3.39(m,1H);3.34(t,2H);3.27(s,4H);3.03(t,2H);2.20-2.17(m,2H);2.07-2.00(m,2H);1.74(d,2H);1.58(d,3H);1.39-1.33(m,2H);1.30-1.24(m,2H);1.09-1.03(m,1H).ESI-MS:348.
Embodiment 7
(S)-1-(2-(2-(3-normal-butyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 7.
(S) preparation of-1-(2-(2-(3-normal-butyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate is prepared by ordinary method with reference to embodiment mono-.
1H-NMR?δ(DMSO-d
6):4.83(dd,1H);4.04-4.01(m,2H);3.61-3.57(m,1H);3.43-3.39(m,1H);3.60(t,2H);3.29(s,4H);3.08-3.05(m,4H);2.20-2.17(m,2H);2.07-2.03(m,2H).ESI-MS:322。
Embodiment 8
(S)-1-(2-(2-(3-(3-fluorophenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 8.
(S) preparation of-1-(2-(2-(3-(3-fluorophenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate is prepared by ordinary method with reference to embodiment mono-.
1H-NMRδ(DMSO-d
6):7.44(d,1H);7.38-7.34(m,1H);7.31(d,1H);6.81(t,1H);4.82(dd,1H);4.01-3.91(m,2H);3.83(t,2H);3.62-3.59(m,1H);3.51(t,4H);3.44-3.40(m,1H);3.09(t,2H);2.20-2.16(m,2H);2.07-2.02(m,2H).ESI-MS:360.
Embodiment 9
(S)-1-(2-(2-(3-(4-fluorophenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 9.
(S) preparation of-1-(2-(2-(3-(4-fluorophenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate is prepared by ordinary method with reference to embodiment mono-.
1H-NMRδ(DMSO-d
6):7.60-7.58(m,2H);7.17(t,2H);4.83(dd,1H);4.05-3.95(m,2H);3.80(t,2H);3.72-3.67(m,1H);3.51-3.48(m,4H);3.45-3.40(m,1H);3.11(s,2H);2.20-2.16(m,2H);2.10-2.00(m,2H).ESI-MS:360.
Embodiment 10
(S)-1-(2-(2-(3-(2-phenylethyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 10.
(S) preparation of-1-(2-(2-(3-(2-phenylethyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate is prepared by ordinary method with reference to embodiment mono-.
1H-NMR?δ(DMSO-d
6):7.28(t,2H);7.23(d,2H);7.19(t,1H);4.82(dd,1H);4.00-3.88(m,2H);3.59-3.56(m,1H);3.42-3.38(m,1H);3.34-3.30(m,4H);3.27(s,4H);3.00(t,2H);2.75(t,2H);2.18-2.15(m,2H);2.07-2.00(m,2H).ESI-MS:370.
Embodiment 11
(S)-1-(2-(2-(3-(3,4-Dimethoxyphenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 11.
(S) preparation of-1-(2-(2-(3-(3,4-Dimethoxyphenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate is prepared by ordinary method with reference to embodiment 1.
1H-NMRδ(DMSO-d
6):7.46(d,1H);6.91(d,1H);6.85(dd,1H);4.82(dd,1H);4.08-4.00(m,2H);3.78(t,2H);3.74(s,3H);3.71(s,3H);3.62-3.58(m,1H);3.52-3.48(m,4H);3.44-3.40(m,1H);3.09(t,2H);2.19-2.16(m,2H);2.08-1.98(m,2H).ESI-MS:402.
Embodiment 12
(S)-1-(2-(2-(3-(3,5-Dimethoxyphenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate, the compounds of this invention 12.
(S) preparation of-1-(2-(2-(3-(3,5-Dimethoxyphenyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine oxalate is prepared by ordinary method with reference to embodiment 1.
1H-NMR?δ(DMSO-d
6):7.46(S,1H);6.91(S,1H);6.85(S,1H);4.82(dd,1H);4.08-4.00(m,2H);3.79(t,2H);3.76(s,3H);3.71(s,3H);3.62-3.59(m,1H);3.52-3.48(m,4H);3.43-3.40(m,1H);3.08(t,2H);2.19-2.16(m,2H);2.07-1.98(m,2H).ESI-MS:402.
Embodiment 13
(S)-1-(2-(2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine hydrochloride, the compounds of this invention 13.
A 1-(4-methoxybenzyl)-2-imidazolidone
Quadrol (24g) is dissolved in methyl alcohol, under ice bath is cooling, adds p-tolyl aldehyde (27g), then gradation adds sodium borohydride (7.6g), insulated and stirred is spent the night, concentrating under reduced pressure except methyl alcohol after, with methylene dichloride dilution, saturated brine washs once, anhydrous sodium sulfate drying, add again triethylamine (30g), be cooled to, after subzero 30 degree, drip triphosgene (20g), saturated brine washing, to clean, is dried the concentrated crude product that to obtain after completion of the reaction.Silicagel column is separated, obtains 1-(4-methoxybenzyl)-2-imidazolidone (15g).
B 2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) methyl acetate
Sodium hydride (1.8g) is suspended in tetrahydrofuran (THF), cooling 1-(4-the methoxybenzyl)-2-imidazolidone (15g) that adds of ice bath, room temperature reaction drips methyl bromoacetate (16.4g) after 1 hour, stirring is spent the night, use ethyl acetate dilute reaction solution, saturated brine washing, to clean, is dried the concentrated crude product that to obtain, and petrol ether/ethyl acetate is crossed post and obtained 2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) methyl acetate (8g).
C 1-(4-mehtoxybenzyl)-3-(2-hydroxyethyl)-2-imidazolidone
2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) methyl acetate (2.78g) is dissolved in Virahol, under cryosel bath is cooling, add sodium borohydride (0.38g), stirring is spent the night, concentrating under reduced pressure is removed Virahol, with methylene dichloride, dilute again, saturated brine washing three times, anhydrous sodium sulfate drying, concentrating under reduced pressure obtains crude product (2.38g) .ESI-MS:251.
D methylsulfonyl 2-(3-(4-methoxybenzyl)-2-oxo-1-imidazolidyl) ethyl ester
1-(4-methoxybenzyl)-3-(2-hydroxyethyl)-2-imidazolidone (2.38g) is dissolved in 20mL methylene dichloride, add triethylamine (2g), under cryosel bath is cooling, add methylsulfonyl chloride (1.15g), stir 1 hour, reaction solution dilutes with methylene dichloride, saturated brine washing three times, anhydrous sodium sulfate drying, concentrating under reduced pressure obtains crude product (3.12g).
E, (S)-1-(2-(2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine hydrochloride
Methylsulfonyl 2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) ethyl ester (3.12g) and (S)-1-(2-glycyl)-2-Cyanopyrolidine trifluoroacetate (2.67g) are dissolved in to 30mL DMF; add potash solid (3.5g; 0.025mol); be heated to 50 degree reaction 15 hours; reaction solution dilutes with methylene dichloride; with saturated sodium bicarbonate solution, saturated brine, respectively wash three times; anhydrous sodium sulfate drying, concentrating under reduced pressure obtains crude product.Silicagel column is separated, trichloromethane: methyl alcohol (95:5) wash-out obtains (S)-1-(2-(2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine (0.7g).Be dissolved in Virahol again, splash into equimolar ethanol solution of hydrogen chloride, filtration drying obtains white solid (0.6g).
1H-NMR?δ(CDCl
3):7.18(d,2H);6.90(d,2H);4.83(dd,1H);4.21(s,2H);4.01-3.91(m,2H);3.73(s,3H);3.60-3.57(m,1H);3.42-3.40(m,1H);3.40(t,2H);3.30(t,2H);3.15(t,2H);3.05(t,2H);2.19-2.17(m,2H);2.06-2.02(m,2H).ESI-MS:386.
Embodiment 14
(S)-1-(2-(2-(3-phenmethyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine hydrochloride, the compounds of this invention 14.
(S) preparation method of-1-(2-(2-(3-phenmethyl-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine hydrochloride is prepared by ordinary method with reference to embodiment 11.
1H-NMR?δ(CDCl
3):7.33-7.24(m,5H);4.75(dd,1H);4.36(s,2H);3.62-3.59(m,1H);3.47(s,2H);3.44-3.40(m,1H);3.38-3.30(m,2H);3.20(t,2H);2.87-2.80(m,2H);2.31-2.25(m,2H);2.6-2.12(m,2H);1.99(br，1H).ESI-MS:356,378。
Embodiment 15
(S)-1-(2-(2-(3-(4-methylbenzene methyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine hydrochloride, the compounds of this invention 15.
(S) preparation method of-1-(2-(2-(3-(4-methylbenzene methyl)-2-oxo-1-imidazolidyl) ethylamino) ethanoyl)-2-Cyanopyrolidine hydrochloride is prepared by ordinary method with reference to embodiment 11.
1H-NMRδ(DMSO-d
6):7.16-7.13(m,4H);4.83(dd,1H);4.24(s,2H);4.01-3.92(m,2H);3.59-3.57(m,1H);3.42-3.39(m,3H);3.30(t,2H);3.16(t,2H);3.05(t,2H);2.28(s,3H);2.19-2.17(m,2H);2.07-2.00(m,2H).ESI-MS:370.
Embodiment 16
(S)-1-(2-(2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) kharophen) ethanoyl)-2-Cyanopyrolidine, the compounds of this invention 16.
2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) methyl acetate (2.78g) is dissolved in 25mL ethanol, add sodium hydroxide solution, stirring at room 1 hour, with 1N hydrochloric acid, regulating PH is 4, by ethyl acetate, dilute, saturated brine washing three times, anhydrous sodium sulfate drying, concentrating under reduced pressure 2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) acetic acid obtains crude product (2.64g).
2-(3-(4-mehtoxybenzyl)-2-oxo-1-imidazolidyl) acetic acid (2.64g) is dissolved in methylene dichloride, add thionyl chloride (23.8g), reflux 2 hours, concentrating under reduced pressure is removed unnecessary thionyl chloride, with methylene dichloride, dilute again, splashed into the dichloromethane solution of (S)-1-(2-glycyl)-2-Cyanopyrolidine trifluoroacetate (2.67g) and triethylamine (3.03g), stir 3 hours, reaction solution saturated sodium bicarbonate solution, 1N hydrochloric acid soln, saturated brine respectively washs three times, anhydrous sodium sulfate drying, concentrating under reduced pressure obtains crude product.Silicagel column is separated, trichloromethane: methyl alcohol (100:1) wash-out obtains product (2.0g).
1H-NMR?δ(CDCl
3):8.11(t,1H);7.16(d,2H);6.88(d,2H);4.73(dd,1H);4.20(s,2H);3.93(dd,1H);3.77(s,2H);3.71(s,3H);3.63-3.59(m,1H);3.44(dd,1H);3.31(t,2H);3.13(t,2H);2.15-2.10(m,2H);2.05-1.98(m,2H).ESI-MS:400,422.
Embodiment 17
(S)-1-(2-(2-(3-(3,4-methylene-dioxy phenmethyl)-2-oxo-1-imidazolidyl) kharophen) ethanoyl)-2-Cyanopyrolidine, the compounds of this invention 17.
(S) preparation of-1-(2-(2-(3-(3,4-methylene-dioxy phenmethyl)-2-oxo-1-imidazolidyl) kharophen) ethanoyl)-2-Cyanopyrolidine, with reference to embodiment 11 and 14, is prepared by ordinary method.
1H-NMRδ(CDCl
3):7.06(s,1H);6.79-6.73(m,3H);5.94(s,2H);4.75(dd,1H);4.31(s,2H);4.15(dd,1H);3.98(dd,1H);3.93(s,2H);3.64-3.61(m,1H);3.49-3.42(m,1H);3.44-3.38(m,2H);3.28(t,2H);2.33-2.26(m,2H);2.22-2.18(m,2H).ESI-MS:414.
The preparation of embodiment 18 the compounds of this invention 2 oral tablets
Get the compounds of this invention 2 preparing appropriate, pulverize, mix in right amount with Microcrystalline Cellulose, polyvidone, croscarmellose sodium, Magnesium Stearate, stir, direct compression, obtains the oral tablet of the compounds of this invention 2.
The preparation of embodiment 19 the compounds of this invention 10 oral capsules
Get the compounds of this invention 10 preparing appropriate, pulverize, mix with Microcrystalline Cellulose, Magnesium Stearate, stir, divide and be filled in No. 3 capsule shell, seal, obtain the oral capsule of the compounds of this invention 10.
By the test of pesticide effectiveness, further illustrate beneficial effect of the present invention below.
Test example 1 the compounds of this invention is to adding sample and HEPES damping fluid (the 25mM HEPES of appropriate DPP-IV enzyme (SIGMA), 3 times of concentration gradient dilutions in the vitro detection test reaction system of DPP-IV enzyme inhibition activity, 140Mm NaCl, 1%BSA, 80mM MgCl
2), set up blank (not containing enzyme and sample), negative control (not containing sample) and positive control (positive control medicine is KR-62436 simultaneously, SIGMA), room temperature reaction 10min, add substrate Gly-Pro-7-amido-4-methylcoumarin(SIGMA), room temperature lucifuge reaction 20min, detects fluorescence, excitation wavelength 355nm, emission wavelength 460nm.According to fluorescence measured value, calculate inhibiting rate, inhibiting rate=[1-(sample-blank)/(feminine gender-blank)] * 100%, the 4Parameter iLogistic Model in application Xlfit software calculates IC50.Numbering 1～17 compound compound that corresponding embodiment 1 to embodiment 17 prepares respectively.
Table: Compound D PP-IV determination of activity result
Note: KR-62436 structural formula and reference paper are as follows:
Reference:
Kwang-RokKim，Etc.KR-62436，6-{2-[2-(5-cyano-4，5-dihydropyrazol-1-yl)-2-oxoethylamino]ethylamino}nicotinonitrile，is?a?novel?dipeptidyl?peptidase-IV(DPP-IV)inhibitor?with?anti-hyperglycemic?activity,Eur?J?Pharmacol?2005，Jul?25；518(1)：63-70
Known to the vitro detection test of DPP-IV enzyme inhibition activity by the compounds of this invention, KR-62436 compares with positive control medicine, the effect all with inhibition DPP-IV enzymic activity of chemical combination of the present invention, wherein 1,5,9,10,11,12,14 7 compounds of compound and all the other Compound Phase ratios, have obviously preferably actively, especially the activity of compound 11,12 is best.
The hypoglycemic experimental data of test example 2 the compounds of this invention to diabetic mice animal model
1 ﹑ method:
Laboratory animal: II type Spontaneous Diabetic mouse, body weight 25～30g, male and female half and half.Mouse adaptability is raised to a Zhou Tianhou, after fasting 14h, utilize Roche blood glucose meter to measure fasting plasma glucose (basic blood sugar before administration), according to fasting blood sugar random packet, group is model Dui Zhao Zu ﹑ positive controls (Vildagliptin bulk drug) ﹑ DPP-IV inhibitor 1(the compounds of this invention 15) Zu ﹑ DPP-IV inhibitor 2(the compounds of this invention 12) group and DPP-IV inhibitor 3(the compounds of this invention 1) group; Alternative uses C57BL/6J mouse as blank group.
Test method: each is organized mouse and is 14 according to the form below 1 design dosage single-doses respectively, blank group and model control group give corresponding distilled water, 45min after administration, the dextrose anhydrous of gavage 2.0g/kg, and 30min ﹑ 60min ﹑ 120min measures blood sugar and blood sugar increasing rate thereof after glucose load.
Complete after single-dose carbohydrate tolerance test, start successive administration 3 weeks, dosage is the same.At successive administration after 2 weeks, measure 1h blood sugar after fasting plasma glucose and medicine;
Continuously in administration after 3 weeks, complete carbohydrate tolerance test after multiple dosing.
(table 1) mouse test dosage and grouping
2 ﹑ test-results:
(1) single-dose carbohydrate tolerance test (in Table 2)
Carbohydrate tolerance test blood sugar increasing rate (X ± S) after table 2 single-dose
Note: and model control group comparison, * P < 0.05, * * P < 0.01; With the comparison of positive group, ▲ P < 0.05.
Experimental result shows:
Blank group and model control group comparison, after the dextrose anhydrous of gavage 2g/kg, after 30min ﹑ 60min and 120min, blood sugar increasing rate is all significantly raised (* * P ﹤ 0.01, * P ﹤ 0.05) compared with blank group.
With model control group comparison, positive controls (Vildagliptin) 30min ﹑ 60min and 120min after the dextrose anhydrous of gavage 2g/kg all can significantly reduce blood sugar increasing rate (* P ﹤ 0.05), show that this positive drug can reduce postprandial blood sugar.
With model group comparison, 1,2,3 groups, DPP-IV inhibitor 30min ﹑ 60min and 120min after the dextrose anhydrous of gavage 2g/kg also all can significantly reduce blood sugar increasing rate (* * P ﹤ 0.01, * P ﹤ 0.05), show that these 3 compounds can reduce Spontaneous Diabetic mouse postprandial blood sugar, 120min after glucose load, all can make mouse blood sugar substantially return to level before the meal.
Data show that the compounds of this invention 15 ﹑ 12 and compound 1 all can significantly reduce Spontaneous Diabetic mouse postprandial blood sugar; Compare with positive group (Vildagliptin), after glucose load 30min, the compounds of this invention 12 and compound 1 reduce blood sugar more obvious (▲ P < 0.05); In general, the compounds of this invention 15 ﹑ 12 and compound 1, curative effect is all better than positive drug (Vildagliptin), and DPP-IV inhibitor group 2(the compounds of this invention 12) more outstanding.
(1h blood sugar (in Table 3) after the rear fasting plasma glucose of 2) ﹑ administrations 2 weeks and medicine
Blood sugar after fasting plasma glucose and medicine after table 32 weeks
Note: and model group comparison, * P<0.05, * * P<0.01; With the comparison of positive group, ▲ P < 0.05, ▲ ▲ P < 0.01.
Test-results shows:
In gavage after 2 weeks, blank group and model control group comparison, fasting plasma glucose and give distilled water after 1h blood glucose value all significantly reduce (* * P<0.01, * P<0.05), show that Spontaneous Diabetic mouse model is comparatively stable.
Positive controls and model group comparison, after fasting plasma glucose and medicine, 1h blood glucose value all significantly reduces (* P<0.05), shows that Vildagliptin is in administration after 2 weeks, can reduce blood glucose value after the medicine after diabetic mice fasting plasma glucose and multiple dosing.
With model group comparison, 1,2,3 groups, DPP-IV inhibitor is in administration after 2 weeks, also can significantly reduce 1h blood sugar after fasting plasma glucose and medicine, show that DPP-IV inhibitor is after administration for some time, can reduce blood glucose value after the medicine after Spontaneous Diabetic mouse fasting plasma glucose and multiple dosing.
Compare with positive controls (Vildagliptin) simultaneously, 2,3 groups of more obvious (▲ P < 0.05 of reduction blood sugar of DPP-IV inhibitor, ▲ ▲ P < 0.01), in addition the blood sugar concentration of DPP-IV inhibitor group 1 mouse also lower than positive controls.
Data show: the test-compound of DPP-IV inhibitor group 1,2,3 can significantly reduce blood sugar after fasting plasma glucose and medicine after multiple dosing, and its blood sugar reducing function is better than positive drug (Vildagliptin).In general effect, DPP-IV inhibitor group 2(the compounds of this invention 12) more outstanding, DPP-IV inhibitor group 3(the compounds of this invention 1) is taken second place.
(after 3) ﹑ administrations (3 weeks), sugar tolerance changes (in Table 4)
Carbohydrate tolerance test blood sugar increasing rate (X ± S) after (table 4) multiple dosing (3 weeks)
Note: with model group comparison, * p<0.05, * * P<0.01; With the comparison of positive group, ▲ P < 0.05, ▲ ▲ P < 0.01.
Test-results shows:
As can be known from the above table, 30min, 60min and 120min after glucose load, 1,2,3 groups of mouse blood sugar levels of DPP-IV inhibitor are all significantly lower than model group (* * P<0.01, * P<0.05); Show after multiple dosing 1,2,3 groups of level of postprandial blood sugar that all can significantly reduce II type Spontaneous Diabetic mouse of DPP-IV inhibitor.
With the comparison of Vildagliptin positive controls, DPP-IV inhibitor group 2 mouse are after load grape 30min, 60min, 120min, and glucose level this positive group significantly reduces (▲ ▲ P < 0.01); DPP-IV inhibitor group 3 mouse are after load glucose 30min, 60min, 120min, and glucose level this positive group significantly reduces (▲ P < 0.05); In addition the blood level of DPP-IV inhibitor group 1 mouse this positive group also decrease.
Experimental result shows: the test-compound of DPP-IV inhibitor group 1,2,3 can significantly reduce postprandial blood sugar after multiple dosing, and its blood sugar reducing function is better than positive drug (Vildagliptin).In general effect, DPP-IV inhibitor group 2(the compounds of this invention 12) more outstanding, DPP-IV inhibitor group 3(the compounds of this invention 1) is taken second place.
(4) body weight change (in Table 5) before and after ﹑ administration
21 days body weight change (X ± S) of 14 days ﹑ before table 5 administration and after administration
Note: compare * p<0.05, * * P<0.01 with model
Compare with model group, the body weight of Vildagliptin positive controls and 1,2,3 groups of mouse of DPP-IV inhibitor does not all occur significantly to increase, and shows that these tested medicines do not have significant impact to Mouse Weight in the glucose level that reduces diabetic mice.
3, conclusion
Experimental data clearly shows that the compounds of this invention has definite effect to reducing fasting plasma glucose and postprandial blood sugar, wherein reduces postprandial blood sugar more obvious, and after multiple dosing, does not also cause the increase of body weight.From synthesis result, the compounds of this invention drug effect is better than Vildagliptin; DPP-IV inhibitor group 2(the compounds of this invention 12 wherein) more outstanding, DPP-IV inhibitor group 3(the compounds of this invention 1) take second place.
Claims (2)
1. a preparation method of general formula compound I, comprises the following steps:
And in general formula compound I
X is methylene radical;
N is 1;
R is selected from C
1-C
8alkyl, phenyl, and on one or more carbon atoms of above-mentioned group, be substituted and substituting group is chosen from C
1-C
8alkyl, C
1-C
8alkoxyl group;
(1) take Boc-glycine and proline methyl ester as raw material, take DCC and HOBT as dewatering agent, the sweet dried meat methyl esters of preparation formula IIBoc-; Formula II solves the sweet dried meat acid amides of formula III Boc-with the saturated methanol solution ammonia of ammonia, and formula III is through the phosphorus oxychloride formula IV that dewaters to obtain, and formula IV obtains intermediate formula V through trifluoroacetic acid Deprotection
(2) with formula XI aldehyde and reacting ethylenediamine, use again the monosubstituted quadrol of sodium borohydride reduction production XII; formula XII is cyclisation production X III substituted imidazole alkane ketone under the effect of phosphinylidyne imidazoles; methyl bromoacetate hydrocarbonylation production X IV for formula X III; formula X IV reduces to obtain formula XV, after formula XV methylsulfonyl, obtains general formula compound I again with the coupling of the sweet dried meat dipeptidase derivant of formula V
2. preparation method according to claim 1, is characterized in that in general formula compound I, n is that 1, R is 4-methoxyphenyl.
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| CN1385421A (en) * | 2001-05-15 | 2002-12-18 | 瑟维尔实验室 | Novel alpha-amino acid compound, its preparation process and medicinal composition contaiing same |
| CN1890228A (en) * | 2003-12-11 | 2007-01-03 | 三菱制药株式会社 | Alpha-amino acid derivatives and use thereof as medicines |
| CN1905868A (en) * | 2003-12-09 | 2007-01-31 | 桑塞拉瑞士制药有限公司 | DPP-IV inhibitors |
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| CN1385421A (en) * | 2001-05-15 | 2002-12-18 | 瑟维尔实验室 | Novel alpha-amino acid compound, its preparation process and medicinal composition contaiing same |
| CN1905868A (en) * | 2003-12-09 | 2007-01-31 | 桑塞拉瑞士制药有限公司 | DPP-IV inhibitors |
| CN1890228A (en) * | 2003-12-11 | 2007-01-03 | 三菱制药株式会社 | Alpha-amino acid derivatives and use thereof as medicines |
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