CN102838581A - Method for extracting rhinacanthin from Rhinacanthus nasutus (L.) Lindau - Google Patents
Method for extracting rhinacanthin from Rhinacanthus nasutus (L.) Lindau Download PDFInfo
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- CN102838581A CN102838581A CN2012103448491A CN201210344849A CN102838581A CN 102838581 A CN102838581 A CN 102838581A CN 2012103448491 A CN2012103448491 A CN 2012103448491A CN 201210344849 A CN201210344849 A CN 201210344849A CN 102838581 A CN102838581 A CN 102838581A
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- twig
- leaf
- extraction
- bignose rhinacanthus
- rhinacanthus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
The invention discloses a method for extracting rhinacanthin from Rhinacanthus nasutus (L.) Lindau, which comprises the following steps of: using Rhinacanthus nasutus (L.) Lindau as raw materials, drying and crushing the Rhinacanthus nasutus (L.) Lindau, passing the powder through a 20-mesh to 80-mesh sieve, putting the screened powder into an extraction kettle, feeding liquid CO2 into the extraction kettle for supercritical extraction, dissolving the obtained extract by using ethanol solution, conducting separation by using an alumina resin column, collecting lower column liquid, extracting by using petroleum ether, collecting an ethanol solution layer and recovering reagent to obtain the rhinacanthin. The method for extracting rhinacanthin from Rhinacanthus nasutus (L.) Lindau has the advantages that the extraction efficiency is high, the operation steps are simple, the environmental protection is facilitated and the method is suitable for industrial production.
Description
Technical field
The invention belongs to the Natural Medicine Chemistry field, particularly a kind of plain method of Twig and leaf of Bignose Rhinacanthus that from Twig and leaf of Bignose Rhinacanthus, extracts.
Background technology
Supercritical CO
2The extracting and separating principle is under supercritical state, with supercritical CO
2Contact with treating isolating material, it is extracted the composition of polarity size, boiling point height and molecular weight size selectively successively.Because whole process is without organic solvent, so the exhausted noresidue solvent of extract, also prevented that leaching process to the murder by poisoning of human body with to the pollution of environment, being 100% pure natural simultaneously, and CO
2Low price, purity is high, obtains easily, and recycles in process of production.
Twig and leaf of Bignose Rhinacanthus
Rhinacanthus nasutus(L.) Lindau is an acanthaceous plant, and another name tinea grass has the function that fire, killing parasites for relieving itching fall in moistening lung, cures mainly diseases such as pulmonary tuberculosis, oedema due to nephritis, ringworm of the body, eczema.Modern study is found, contains multiple naphthoquinone compound in the Twig and leaf of Bignose Rhinacanthus, and like the plain A of Twig and leaf of Bignose Rhinacanthus, the plain B of Twig and leaf of Bignose Rhinacanthus, the plain C of Twig and leaf of Bignose Rhinacanthus, Twig and leaf of Bignose Rhinacanthus D etc., this compounds has than the powerful antitumor effect.
Through literature search, find to have the industrial method of the method for extraction Twig and leaf of Bignose Rhinacanthus element from Twig and leaf of Bignose Rhinacanthus.
Summary of the invention
The purpose of this invention is to provide a kind of plain method of Twig and leaf of Bignose Rhinacanthus of from Twig and leaf of Bignose Rhinacanthus, extracting, this method is efficient, easy, is fit to industriallization.
To achieve these goals, the present invention adopts following technical scheme:
A kind of plain method of Twig and leaf of Bignose Rhinacanthus that from Twig and leaf of Bignose Rhinacanthus, extracts, it is characterized in that: be raw material with the Twig and leaf of Bignose Rhinacanthus, crushed after being dried is crossed 20 ~ 80 mesh sieves and is dropped into extraction kettle, feeds liquid CO
2Carry out supercritical extraction, the gained extract is used ethanolic soln solution, and peroxo-aluminium resin column is collected lower column liquid, uses petroleum ether extraction, collects the ethanolic soln layer, reclaims reagent and promptly gets.
Described supercritical CO
2Extraction conditions is: extraction temperature is that 40 ~ 50 ℃, pressure are that 15 ~ 25MPa, time are 3 ~ 4h, CO
2Flow is 20 ~ 25L/h, 50 ~ 60 ℃ of resolution temperatures, pressure 5 ~ 10MPa.
Described aluminum oxide is a neutral alumina, granularity 120 ~ 300 orders.
Positively effect of the present invention is: method is that to utilize the Twig and leaf of Bignose Rhinacanthus element all be volatile character, adopts supercritical CO
2Extraction, method is efficient, quick, and does not produce solvent contamination and waste liquid, is beneficial to environmental protection.
Embodiment:
Embodiment 1:
The Twig and leaf of Bignose Rhinacanthus medicinal material, crushed after being dried is crossed 40 mesh sieves, gets 2kg and drops in the extraction kettle, feeds the CO that is in supercritical state
2Extract, set the extraction kettle parameter: 45 ℃ of extraction temperature, pressure are 20MPa, feed CO
2Flow is 20L/h, and dynamic extraction 4h resolves extract at 60 ℃ of temperature, pressure 8MPa from separating still.The gained extract is used 70% dissolve with ethanol solution, crosses neutral alumina (granularity 180-200 order) short column, collects lower column liquid; Change adding equal-volume sherwood oil in the separating funnel over to, thorough mixing discards petroleum ether layer after the layering; Extract 3 times; Change in the Rotary Evaporators, concentrating under reduced pressure gets the plain 3.1g of oily matter Twig and leaf of Bignose Rhinacanthus.
Embodiment 2:
The Twig and leaf of Bignose Rhinacanthus medicinal material, crushed after being dried is crossed 80 mesh sieves, gets 2kg and drops in the extraction kettle, feeds the CO that is in supercritical state
2Extract, set the extraction kettle parameter: 40 ℃ of extraction temperature, pressure are 25MPa, feed CO
2Flow is 25L/h, and dynamic extraction 3h resolves extract at 50 ℃ of temperature, pressure 7MPa from separating still.The gained extract is used 80% dissolve with ethanol solution, crosses neutral alumina (granularity 120-200 order) short column, collects lower column liquid; Change adding equal-volume sherwood oil in the separating funnel over to, thorough mixing discards petroleum ether layer after the layering; Extract 2 times; Change in the Rotary Evaporators, concentrating under reduced pressure gets the plain 3.5g of oily matter Twig and leaf of Bignose Rhinacanthus.
Embodiment 3:
The Twig and leaf of Bignose Rhinacanthus medicinal material, crushed after being dried is crossed 20 mesh sieves, gets 2kg and drops in the extraction kettle, feeds the CO that is in supercritical state
2Extract, set the extraction kettle parameter: 45 ℃ of extraction temperature, pressure are 15MPa, feed CO
2Flow is 20L/h, and dynamic extraction 3h resolves extract at 55 ℃ of temperature, pressure 7MPa from separating still.The gained extract is used 75% dissolve with ethanol solution, crosses neutral alumina (granularity 180-200 order) short column, collects lower column liquid; Change adding equal-volume sherwood oil in the separating funnel over to, thorough mixing discards petroleum ether layer after the layering; Extract 3 times; Change in the Rotary Evaporators, concentrating under reduced pressure gets the plain 4.2g of oily matter Twig and leaf of Bignose Rhinacanthus.
Claims (3)
1. one kind extracts the plain method of Twig and leaf of Bignose Rhinacanthus from Twig and leaf of Bignose Rhinacanthus, it is characterized in that: be raw material with the Twig and leaf of Bignose Rhinacanthus, crushed after being dried is crossed 20 ~ 80 mesh sieves and is dropped into extraction kettle, feeds liquid CO
2Carry out supercritical extraction, the gained extract is used ethanolic soln solution, and peroxo-aluminium resin column is collected lower column liquid, uses petroleum ether extraction, collects the ethanolic soln layer, reclaims reagent and promptly gets.
2. the plain method of Twig and leaf of Bignose Rhinacanthus that from Twig and leaf of Bignose Rhinacanthus, extracts according to claim 1 is characterized in that described supercritical CO
2Extraction conditions is: extraction temperature is that 40 ~ 50 ℃, pressure are that 15 ~ 25MPa, time are 3 ~ 4h, CO
2Flow is 20 ~ 25L/h, 50 ~ 60 ℃ of resolution temperatures, pressure 5 ~ 10MPa.
3. the plain method of Twig and leaf of Bignose Rhinacanthus that from Twig and leaf of Bignose Rhinacanthus, extracts according to claim 1 is characterized in that described aluminum oxide is a neutral alumina, granularity 120 ~ 300 orders.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103448491A CN102838581A (en) | 2012-09-18 | 2012-09-18 | Method for extracting rhinacanthin from Rhinacanthus nasutus (L.) Lindau |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103448491A CN102838581A (en) | 2012-09-18 | 2012-09-18 | Method for extracting rhinacanthin from Rhinacanthus nasutus (L.) Lindau |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102838581A true CN102838581A (en) | 2012-12-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012103448491A Pending CN102838581A (en) | 2012-09-18 | 2012-09-18 | Method for extracting rhinacanthin from Rhinacanthus nasutus (L.) Lindau |
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| CN (1) | CN102838581A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115385795A (en) * | 2022-10-11 | 2022-11-25 | 广西中医药大学赛恩斯新医药学院 | Preparation method and application of rhinacanthus nasutus and ganoderma naphthoquinone monomer rhinacanthin-C |
-
2012
- 2012-09-18 CN CN2012103448491A patent/CN102838581A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115385795A (en) * | 2022-10-11 | 2022-11-25 | 广西中医药大学赛恩斯新医药学院 | Preparation method and application of rhinacanthus nasutus and ganoderma naphthoquinone monomer rhinacanthin-C |
| CN115385795B (en) * | 2022-10-11 | 2023-11-17 | 广西中医药大学赛恩斯新医药学院 | Preparation method and application of Rhinacanthus nasuta (L.) kurz naphthoquinone monomer Rhinacanthin-C |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20121226 |