CN102836182A - Extraction process of Ganoderma lucidum acidic components - Google Patents
Extraction process of Ganoderma lucidum acidic components Download PDFInfo
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- CN102836182A CN102836182A CN 201110167994 CN201110167994A CN102836182A CN 102836182 A CN102836182 A CN 102836182A CN 201110167994 CN201110167994 CN 201110167994 CN 201110167994 A CN201110167994 A CN 201110167994A CN 102836182 A CN102836182 A CN 102836182A
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- ganoderma
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- ganoderma lucidum
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Abstract
The invention relates to an extraction process of Ganoderma lucidum acidic components. The process employs an ultrasound-assisted alkaline method to extract acidic components in Ganoderma lucidum. According to the process, researches on different processes for separating and extracting Ganoderma lucidum acidic components are carried out, with amount of the obtained Ganoderma lucidum acidic substances and content of Ganoderma lucidum acid A as test indexes, so as to provide an ideal extraction process of Ganoderma lucidum acid A. Comparison on three extraction methods, including a chloroform-ethanol extraction method, an ultrasonic assisted alkali extraction method and a water bath auxiliary alkaline extraction method, is carried out.
Description
Technical field:
Divide invention to relate to a kind of extraction process of Ganoderma acidic components, the ultrasonic auxiliary alkaline process of this process using extracts the acidic components in the Ganoderma.
Background technology:
Ganoderma is a Basidiomycetes Polyporaceae Ganoderma fungus; In China long medicinal history is arranged; Its acidic components (being mainly the triterpenes Ganodenic acid) have pain relieving, calmness, inhibition histamine release, detoxify, protect the liver, kill functions such as tumor cell, are one of main effective ingredient of Ganoderma.Since nineteen eighty-twos such as Kutoba, separation obtained triterpenes Ganodenic acid A, B from Ganoderma first, separation, purification and the pharmacology activity research of relevant Ganodenic acid was the important content of Ganoderma research always.
The traditional extraction process of ganoderma lucidum triterpene compounds composition generally is to carry out according to its deliquescent difference, and this method need consume a large amount of organic solvents, is prone to cause environmental pollution, and the selectivity that extracts is not high.
Summary of the invention:
The present invention is exactly to the problems referred to above; A kind of extraction process of Ganoderma acidic components is provided; This technology serves as the examination index with the content of the Ganoderma acids amount of substance that obtains and Ganodenic acid A; Studied to the Ganoderma acidic components extract, isolating different process, provide the extraction process of more satisfactory Ganodenic acid A.
1. chloroform-alcohol extracting method
A certain amount of Ganoderma sporophore powder of weighing, three parts, put into the 1000ml triangular flask respectively, in 1: 10-1: 15 (w: v) ratio adds chloroform: dehydrated alcohol (volume ratio 95: 5, down together) extractant, soak 20-25h.Triangular flask is put into ultrasonic cleaner, ultrasonic 30min, stop about 10min after, ultrasonic once more 30min clock.Must filtrate 1 after the filtration.In the filtering residue again in 1: 5-1: 10 (w: v) ratio adds chloroform: the dehydrated alcohol extraction agent, put into ultrasonic cleaner, handle equally, cross and filter filtrating 2.Merging filtrate 1 and 2 is crossed and is filtered supernatant, evaporated under reduced pressure.Use a certain amount of dissolved in chloroform then respectively, with saturated NaHCO
3Solution extraction, with extract under 4 ℃ of condition of ice bath, with the hcl acidifying of 6mol/L to pH3.With the chloroform extraction twice of acidifying solution with the equal-volume ratio, collect chloroform layer, filter, get light yellow clear liquid.This clear liquid is evaporated under reduced pressure under 45 ℃ of conditions, drag visible yellow color thick liquid.Place 60 ℃ of baking ovens to continue dried in flask to constant weight.
2. ultrasonic auxiliary alkali lifting manipulation
A certain amount of Ganoderma sporophore powder of weighing, three parts, put into the 1000ml triangular flask respectively, in 1: 10-1: 15 (w: v) ratio adds 5% (w/v) NaHCO
3Solution soaks 24h.Triangular flask is put into ultrasonic cleaner, ultrasonic 30min, stop about 10min after, ultrasonic once more 30min.Must filtrate 3 after the filtration.In the filtering residue again in 1: 5-1: 10 (w: v) ratio adds 5% (w/v) NaHCO
3Solution is put into ultrasonic cleaner, handles equally, crosses to filter filtrating 4.Merging filtrate 3 and 4, in 5000r/min, 20min gets supernatant.Supernatant is acidified to pH=3 with the hydrochloric acid of 6mol/L under 4 ℃ of condition of ice bath.With the chloroform extraction twice of acidifying solution with the equal-volume ratio, collect chloroform layer, filter, get yellow clear liquid.This clear liquid is evaporated under reduced pressure under 45 ℃ of conditions, drag visible yellow color thick liquid.Place 60 ℃ of baking ovens to continue dried in flask to constant weight.
3. water-bath auxiliary alkali lifting manipulation
Undertaken by above-mentioned ultrasonic auxiliary alkali lifting manipulation, just supplementary means changes 80 ℃ of heating in water bath 1h into by ultrasonic method.
4. Ganodenic acid A content analysis
C
18Reversed phase chromatographic column, mobile phase: 5% acetic acid: methanol=50: 50 (v/v), detect wavelength: 253nm; Sample size: 10 μ l, flow velocity: 0.8ml/min.The preparation of test sample: precision takes by weighing the pure article of Ganodenic acid A, traditional alcohol extracting method and alkali and carries the sample that ultrasonic method is extracted, and is made into the solution of 1 μ g/ μ L respectively with methanol, and 0.45 μ m filtering with microporous membrane is subsequent use.
Respectively with the pure article of Ganodenic acid A, alcohol extracting method and ultrasonic auxiliary alkali lifting manipulation is extracted and behind silica gel column chromatography the sample of gained analyze with the HPLC method.
Beneficial effect of the present invention:
Extraction process of the present invention adopts ultrasonic auxiliary alkaline process that the acidic components in the Ganoderma are extracted, and its Ganodenic acid category-A content of material is very high, and the extraction and separation process of Ganoderma acidic components is easy and simple to handle, cost economy.
The specific embodiment:
The present invention adopts ultrasonic auxiliary alkaline process that the acidic components in the Ganoderma are extracted.This technology serves as the examination index with the content of the Ganoderma acids amount of substance that obtains and Ganodenic acid A, compares with traditional extraction process.
The present invention mainly compares three kinds of extraction methods, is respectively chloroform-alcohol extracting method, ultrasonic auxiliary alkali lifting manipulation and water-bath auxiliary alkali lifting manipulation.
Ultrasonic auxiliary alkali lifting manipulation is extracted triterpenoid compound in the Ganoderma; The target product kind of not only extracting is identical with conventional method; And can extract target product to greatest extent; Wherein the amount with Ganodenic acid A serves as to investigate target, compares with other method for distilling, especially shows the high advantage of extraction efficiency.Simultaneously the process route of ultrasonic auxiliary alkali lifting manipulation has reasonable in design, cost economy, characteristics easy and simple to handle, and the research and development of Ganoderma new product are also had certain facilitation.
Claims (3)
1. the extraction process of Ganoderma acidic components is characterized in that the present invention adopts ultrasonic auxiliary alkaline process that the acidic components in the Ganoderma are extracted.This technology serves as the examination index with the content of the Ganoderma acids amount of substance that obtains and Ganodenic acid A, compares with traditional extraction process;
2. the extraction process of a kind of Ganoderma acidic components according to claim 1 is characterized in that the present invention mainly compares three kinds of extraction methods, is respectively chloroform-alcohol extracting method, ultrasonic auxiliary alkali lifting manipulation and water-bath auxiliary alkali lifting manipulation.
3. the extraction process of a kind of Ganoderma acidic components according to claim 1; It is characterized in that triterpenoid compound in the ultrasonic auxiliary alkali lifting manipulation extraction Ganoderma; The target product kind of not only extracting is identical with conventional method, and can extract target product to greatest extent, and wherein the amount with Ganodenic acid A serves as to investigate target; Compare with other method for distilling, especially show the high advantage of extraction efficiency.Simultaneously the process route of ultrasonic auxiliary alkali lifting manipulation has reasonable in design, cost economy, characteristics easy and simple to handle, and the research and development of Ganoderma new product are also had certain facilitation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110167994 CN102836182A (en) | 2011-06-22 | 2011-06-22 | Extraction process of Ganoderma lucidum acidic components |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110167994 CN102836182A (en) | 2011-06-22 | 2011-06-22 | Extraction process of Ganoderma lucidum acidic components |
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| CN102836182A true CN102836182A (en) | 2012-12-26 |
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| CN 201110167994 Pending CN102836182A (en) | 2011-06-22 | 2011-06-22 | Extraction process of Ganoderma lucidum acidic components |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103724389A (en) * | 2013-12-23 | 2014-04-16 | 福建仙芝楼生物科技有限公司 | Method for separating and preparing anti-tumor components ganoderic acid C1 and ganoderic acid F |
-
2011
- 2011-06-22 CN CN 201110167994 patent/CN102836182A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103724389A (en) * | 2013-12-23 | 2014-04-16 | 福建仙芝楼生物科技有限公司 | Method for separating and preparing anti-tumor components ganoderic acid C1 and ganoderic acid F |
| CN103724389B (en) * | 2013-12-23 | 2015-09-02 | 福建仙芝楼生物科技有限公司 | The method of antineoplastic component ganoderic acid C 1 and Ganodenic acid F is prepared in a kind of separation |
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Application publication date: 20121226 |