CN102827823A - Preparation method of high-strength and high-activity microorganism-embedding carrier - Google Patents
Preparation method of high-strength and high-activity microorganism-embedding carrier Download PDFInfo
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- CN102827823A CN102827823A CN2012103567236A CN201210356723A CN102827823A CN 102827823 A CN102827823 A CN 102827823A CN 2012103567236 A CN2012103567236 A CN 2012103567236A CN 201210356723 A CN201210356723 A CN 201210356723A CN 102827823 A CN102827823 A CN 102827823A
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Abstract
The invention relates to a simple and feasible method for preparing a high-activity and long-life microorganism-embedding carrier at room temperature under the aerobic condition and a formula of the carrier. The microorganism-embedding carrier prepared by the method has high activity and strength and can be used to efficiently remove high-concentration ammonia nitrogen and the pollutants in phenol wastewater; and the service life of the carrier in a fluidized reactor is up to 1.5 years. As the photochemical synthesis method has the characteristics of being performed at room temperature under the aerobic condition and the embedding material has the characteristics of biocompatibility, the method and formula can be widely used in the embeddings of various microorganisms, enzymes, proteins and amicrobic materials and in large-scale industrialization preparation.
Description
Technical field
The present invention relates under the normal temperature aerobic conditions a kind of preparation and corresponding prescription of simple and easy to do HS high reactivity imbedded microbe.The imbedded microbe of this method and formulation is active high, can efficiently remove the pollutent in ammonia nitrogen in high density and the phenolic waste water, and the operation life in fluidized reactor can surpass more than 1.5 years.Because Actinochemical synthesis has under the normal temperature and carries out, aerobic is the characteristics of operation down, and embedded material has the characteristics of biocompatibility, so method and prescription can be widely used in the embedding of multiple mikrobe, enzyme, protein and non-microorganism kind material.But and heavy industrialization preparation.
Background technology
The imbedded microbe technology is owing to have the microbe density height, and anti-external environment impact capacity is strong, and solid-liquid separation is simple, and therefore simple operation and other advantages has very application prospects in fields such as industry such as wastewater treatments.Therefore the shortcoming that but present embedding bacterium ubiquity activity is low, the life-span is short, is difficult to realize industrial applications at present.How in guaranteeing the embedding carrier, on the basis of microorganism active, improve the life-span of carrier, making it in the medium-term and long-term steady running of reactor drum is a present difficult problem.
Summary of the invention
It is not high to the objective of the invention is to overcome existing imbedded microbe activity, the shortcoming that support strength is lower.Through the proportioning between adjustment acrylic amide and the Z 150PH, under the normal temperature aerobic conditions, through photochemistry mikrobe is carried out embedding and fix.Because mikrobe near being embedded under the physiological condition in the carrier, has been kept microbic activity; This carrier can surpass more than 1.5 years the working time in fluidized reactor.This method is simple, convenient, easy handling.
Prescription of the present invention and preparation process are following:
Preparation process one:
1. an amount of acrylic amide is dissolved in the water of a ml, adds a certain amount of methylene-bisacrylamide, be stirred to dissolving, get A solution; 2. in the 60-a of 80-90 ° of C ml deionized water, add a certain amount of Z 150PH, be stirred to dissolving, get B solution;
3. with A and B mixing, add the PBS damping fluid then to wherein being neutrality to it; Add the mikrobe of treating embedding then, stir, get mixed solution C;
4. in mixed solution C, add st-yrax dme solution 0.4ml, Tetramethyl Ethylene Diamine 1.6ml, Potassium Persulphate saturated solution 4ml stirs fast;
5. the reaction solution in 4 was placed under the radiation source irradiation 2-8 minute, be cured;
6. the embedding bacterium after will solidifying as required cuts into corresponding size and size.
Preparation process two:
1. in 80-90 ℃ 60 ml deionized waters, add a certain amount of Z 150PH, be stirred to dissolving, get A solution;
2. an amount of acrylic amide is dissolved in the A solution, adds a certain amount of methylene-bisacrylamide, be stirred to dissolving, get B solution; 3. in B solution, adding the PBS damping fluid is neutral to it; Add the mikrobe of treating embedding then, stir, get mixed solution C;
4. in mixed solution C, add st-yrax dme solution 0.4ml, Tetramethyl Ethylene Diamine 1.6ml, Potassium Persulphate saturated solution 4ml stirs fast;
5. the reaction solution in 4 was placed under the radiation source irradiation 2-8 minute, be cured;
6. the embedding bacterium after will solidifying as required cuts into corresponding size and size.
This kind embedding bacterium preparation method can through light source irradiation, reach transparent system under the normal temperature aerobic conditions, and opaque system and embedding system are carried out photochemistry and efficiently caused, and makes it to solidify, and obtains high reactivity and long-life embedding bacterium.Be specially adapted to embedded immobilization microbial or other abiotic fixed packet embedding system.
This embedding is carried out the photochemistry initiation to transparent, opaque embedding system and is cured embedding, generally comprises following steps:
Step 1 adds 1.8 g-9g Z 150PH in the 60ml of 80-90 ° of C or a ml deionized water, be stirred to dissolving, A solution;
Step 2 adds the acrylamide monomer of 4.8 g-8.4 g and the linking agent of 0.12 g-0.6 g at the A solution of 60 ml of step 1, is stirred to dissolving, solution B; Perhaps
In the deionized water of b ml, add the acrylic amide of 4.8 g-8.4 g and the N of 0.12 g-, 0.6 g, N '-methylene-bisacrylamide is stirred to dissolving, solution A ', a+b=60 wherein;
Step 3 with the A solution of a ml of step 1 and the A ' solution blending of b ml, adds the PBS damping fluid then, to its pH to neutral, solution C; Perhaps
In the B solution of the 60ml of step 2, add the PBS damping fluid, to its to neutral, solution C;
Step 4 adds 1-6 g and treats the embedding thing in solution C, stir, and gets mixed solution D;
Step 5 adds st-yrax dme solution 0.2-0.6ml in mixed solution D, Tetramethyl Ethylene Diamine 0.8-2.8ml, and Potassium Persulphate saturated solution 2-8ml stirs fast;
Step 6 placed the reaction solution that obtains in the step 5 under the radiation source irradiation 2-8 minute, was cured;
Step 7, the embedding bacterium after will solidifying as required cuts into corresponding size and size.
Wherein, in a preferred embodiment, said linking agent is N, N '-methylene-bisacrylamide.
Wherein, in a preferred embodiment, in step 1, the concentration of Z 150PH in solution is: the content of the Z 150PH that contains in per 100 ml solution is 3-15 g.
In a preferred embodiment, in step 2, the concentration of described monomer in solution is: the content of the acrylamide monomer that contains in per 100 ml solution is 8～14 g; N, the N '-methylene-bisacrylamide mass and size percentage ratio in solution be 0.2～1% (m/v, g/ml).
In a preferred embodiment, in step 3, in being dissolved with the reaction solution of reactant, go into the PBS damping fluid, extremely neutral to its pH.
In a preferred embodiment, in step 4, the embedding thing of treating that is added in the reaction solution is active sludge, mikrobe or other embedding object, and the said mass and size percentage ratio of embedding thing in solution of treating is 3.2～20% (m/v).
In a preferred embodiment, in step 5, described Potassium Persulphate, st-yrax dme and the content of Tetramethyl Ethylene Diamine in the solution of step 5 gained are respectively 0.03-0.67% (m/v; G/ml), and 0.1-1.2% (m/v, g/ml); 1-5% (v/v, ml/ml).
In a preferred embodiment, in step 6, said irradiation is 2-8 minute.
In the present invention, said term " transparent system " is meant optically transparent system, includes but not limited to transparent, as can to absorb the photon that light source launches system, like clear solution etc.
In the present invention, said term " opaque system " is meant the opaque system of optics, includes but not limited to, opaque, the system that can not absorb the photon energy that light source launches in a large number.
In the present invention, said term " linking agent " is meant the chemical cross-linking agent that contains unsaturated double-bond, includes but not limited to N, N '-methylene-bisacrylamide.
In the present invention, said term " is treated the embedding thing " and is meant mikrobe, bacterium, enzyme etc., includes but not limited to active sludge, mikrobe (for example nitrifier), enzyme etc.These treat that embedding substance is from wastewater treatment or other chemical biochemical industry field production process.
In the present invention, said term " radiating light source " includes but not limited to ultraviolet source (for example 500W high voltage mercury lamp or other similar irradiation source) etc.
Compared with prior art, the present invention has following beneficial effect:
Embedding bacterium prescription of the present invention not only can be used for the mikrobe or the non-microorganism embedding of transparent system, also applicable to the mikrobe of nontransparent system or the embedding of non-microorganism system.And the embedding bacterium carrier of gained is active high, and the life-span is long, and surpass more than 1.5 years the work-ing life in fluidized reactor.And the photochemical initiators among the present invention is efficient, and photochemical solidification is simply quick, and the time is generally in 4 minutes, and polymerization system is simple, and cost is lower, is suitable for large-scale embedding bacterium preparation.
Specific embodiments
Present embodiment provided detailed embodiment and process, but protection scope of the present invention is not limited to following embodiment being to implement under the prerequisite with technical scheme of the present invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.
Embodiment 1
Step 1, the Z 150PH of adding 3g is stirred to dissolving in 80-90 ℃ 60 ml deionized waters, gets A solution;
Step 2, A solution is cooled to room temperature, adds the 6.5g acrylic amide therein, 0.36g N, N '-methylene-bisacrylamide is stirred to dissolving, gets solution B;
Step 3 joins the PBS damping fluid in the solution B, adjusts its pH for neutral; Add 4g active sludge (Minhang, Shanghai City sewage work provides) then, stir;
Step 4 adds st-yrax dme solution 0.4 ml in the mixed solution that in step 3, obtains, Tetramethyl Ethylene Diamine 1.6ml, and, Potassium Persulphate saturated solution 4ml stirs fast;
Step 5 placed under the radiation source irradiation 4 minutes with the reaction solution that obtains in the step 4, was cured;
Step 6, the embedding bacterium after will solidifying as required cuts into corresponding size and size.
Embodiment 2
Step 1, acrylic amide 6g is dissolved in the 20ml water, adds N, and N '-methylene-bisacrylamide 0.24 g is stirred to dissolving, gets solution A;
Step 2 adds the Z 150PH of 4g in the 40 ml deionized waters of 80-90 ° of C, be stirred to dissolving, B solution;
Step 3 with A and B blend, stirs;
Step 4 joins the PBS damping fluid in the blend solution that step 3 obtains, and adjusts its pH for neutral, adds the 6g active sludge again, stirs;
Step 4 adds st-yrax dme solution 0.4 ml in the mixed solution that in step 3, obtains, Tetramethyl Ethylene Diamine 1.6ml, and Potassium Persulphate saturated solution 4ml stirs fast;
Step 5 placed under the radiation source irradiation 5 minutes with the reaction solution that obtains in the step 4, was cured;
Step 6, the embedding bacterium after will solidifying as required cuts into corresponding size and size.
Embodiment 3
Step 1, the Z 150PH of adding 3g is stirred to dissolving in the 60 ml deionized waters of 80-90 ° of C, gets A solution;
Step 2, A solution is cooled to room temperature, adds the 4.5g acrylic amide therein, 0.36g N, N '-methylene-bisacrylamide is stirred to dissolving, gets solution B;
Step 3 joins the PBS damping fluid in the solution B, adjusts its pH for neutral; (nitrifier and heterotrophic bacteria are with the mixed of 1:1 to add the 4g mixed bacterium then.Wherein, nitrifier is that the thickened sludge of Minhang, Shanghai sewage work is tamed in the 40mg/l ammonia nitrogen waste water and got; Heterotrophic bacteria is the Aerobic Pond thickened sludge of Minhang, Shanghai sewage work), stir;
Step 4 adds st-yrax dme solution 0.5 ml in the mixed solution that in step 3, obtains, Tetramethyl Ethylene Diamine 1.2ml, and Potassium Persulphate saturated solution 4ml stirs fast;
Step 5 placed under the radiation source irradiation 3 minutes with the reaction solution that obtains in the step 4, was cured;
Step 6, the embedding bacterium after will solidifying as required cuts into corresponding size and size.
Embodiment 4
Step 1, the Z 150PH of adding 9g is stirred to dissolving in the 60 ml deionized waters of 80-90 ° of C, gets A solution;
Step 2, A solution is cooled to room temperature, adds the 2.5g acrylic amide therein, 0.18g N, N '-methylene-bisacrylamide is stirred to dissolving, gets solution B;
Step 3 joins the PBS damping fluid in the solution B, adjusts its pH for neutral; Add 4g nitrifier (Minhang, Shanghai sewage work thickened sludge gets through the domestication of 40gm/l ammonia nitrogen waste water) then, stir;
Step 4 adds st-yrax dme solution 0.5 ml in the mixed solution that in step 3, obtains, Tetramethyl Ethylene Diamine 1.2ml, and Potassium Persulphate saturated solution 4ml stirs fast;
Step 5 placed under the radiation source irradiation 3 minutes with the reaction solution that obtains in the step 4, was cured;
Step 6, the embedding bacterium after will solidifying as required cuts into corresponding size and size.
Embodiment 5
Step 1, the Z 150PH of adding 6g is stirred to dissolving in the 60 ml deionized waters of 80-90 ° of C, gets A solution;
Step 2, A solution is cooled to room temperature, adds the 3.5g acrylic amide therein, 0.28g N, N '-methylene-bisacrylamide is stirred to dissolving, gets solution B;
Step 3 joins the PBS damping fluid in the solution B, adjusts its pH for neutral; Add the 4g nitrifier then, stir;
Step 4 adds st-yrax dme solution 0.5 ml in the mixed solution that in step 3, obtains, Tetramethyl Ethylene Diamine 1.2ml, and Potassium Persulphate saturated solution 4ml stirs fast;
Step 5 placed under the radiation source irradiation 3 minutes with the reaction solution that obtains in the step 4, was cured;
Step 6, the embedding bacterium after will solidifying as required cuts into corresponding size and size.
The embodiment of the invention 1,2, the embedding bacterium of preparation has higher activity in 3,4,5.After of short duration domestication (2 week was superior to the embedding bacterium of other prior art, and the domestication of general embedding bacterium is 4-6 week), in the processing of high-concentration ammonia nitrogenous wastewater and micro-polluted water performance good, and have long work-ing life, generally can reach more than 1 year.Wherein the life-span of embodiment 5 and 3 prepared embedding bacterium can reach 1.5 years (the embedding bacterium life-span of other preparation at present generally is no more than 6 months).They are (filling ratio is 10%) in aerobic fluidized reactor, in 7 hours, can reach 96%, 94%, 98%, 93% and 91% respectively to the ammonia nitrogen removal frank of 500mg/l concentration and following ammonia nitrogen waste water.Clearance to COD in the micro-polluted water can reach 75%, 68%, 72%, 65% and 74% respectively, meets or exceeds other embedding bacterium at present.
Although reference example property embodiment is described the present invention, skilled person will appreciate that under the situation that does not depart from scope of the present invention, can carry out various variations and available equivalents substitutes and its corresponding elements.In addition, under the situation that does not depart from base region of the present invention, multiple modification can be carried out so that specific situation or material adapt to instruction of the present invention.
Although through invention has been described with reference to various specific embodiments, will be appreciated that in the spirit of the inventive concept could of describing and scope and can carry out multiple variation.The intent of the present invention is not to be subject to described embodiment, but will have by the defined four corner of the language of appending claims.
Claims (6)
1. the preparation method of a high reactivity HS imbedded microbe carrier is characterized in that:
Step 1 adds 1.8 g-9 g Z 150PH in 60 ml of 80-90 ° of C or a ml deionized water, be stirred to dissolving, A solution;
Step 2 after the A solution cooling room temperature of 60 ml of step 1, adds acrylamide monomer and the 0.1 g-0.7 g linking agent of 4.5 g-9 g therein, is stirred to dissolving, solution B; Perhaps
In the deionized water of b ml, add 4.5 g-9 g acrylamide monomers and 0.1 g-0.7 g linking agent, stirring and dissolving, solution A ', a+b=60 wherein;
Step 3 with the A solution of a ml of step 1 and the A ' solution blending of b ml, adds the PBS damping fluid then, to its pH to neutral, solution C; Perhaps
In the B solution of the 60ml of step 2, add the PBS damping fluid, to its pH to neutral, solution C;
Step 4, what in solution C, add 1-8g treats the embedding thing, stir, mixed solution D;
Step 5, the st-yrax dme solution of adding respective amount in mixed solution D, Tetramethyl Ethylene Diamine, and Potassium Persulphate saturated solution stir rapidly; Wherein the per-cent of Potassium Persulphate in gained solution be 0.03-0.67% (m/v, g/ml), the per-cent of st-yrax dme in solution be 0.1-1.2% (m/v, g/ml), the per-cent of Tetramethyl Ethylene Diamine in solution be 1-5% (v/v, ml/ml);
Step 6 placed the reaction solution that obtains in the step 5 under the radiation source irradiation 2-8 minute, was cured;
Step 7, the embedding bacterium after will solidifying as required cuts into corresponding size and size.
2. the method for claim 1 is characterized in that, said linking agent is N, N '-methylene-bisacrylamide.
3. according to claim 1 or claim 2 method, it is characterized in that the concentration of Z 150PH in end reaction liquid is: the content of the Z 150PH that contains in per 100 ml solution is 3-15 g; The concentration of acrylic amide in end reaction liquid is: the content of the acrylamide monomer that contains in per 100 ml solution is 8～14 g; The mass and size percentage ratio of linking agent in end reaction liquid be 0.2～1% (m/v, g/ml).
4. according to claim 1 or claim 2 method is characterized in that, in step 5; Described Potassium Persulphate, st-yrax dme and the content of Tetramethyl Ethylene Diamine in the solution of step 5 gained are respectively 0.03-0.67% (m/v; G/ml), and 0.1-1.2% (m/v, g/ml); 1-5% (v/v, ml/ml).
5. according to claim 1 or claim 2 method is characterized in that in step 6, said irradiation time is 2-5 minute.
6. the imbedded microbe carrier for preparing according to each described method of claim 1-5.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103539892A (en) * | 2013-10-11 | 2014-01-29 | 上海交通大学 | Hydrogel for embedding treatment and its preparation method and use |
| CN108726667A (en) * | 2018-06-05 | 2018-11-02 | 佛山皖阳生物科技有限公司 | A kind of preparation method of hydrogel Microorganism-embeddcarrier carrier |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103539892A (en) * | 2013-10-11 | 2014-01-29 | 上海交通大学 | Hydrogel for embedding treatment and its preparation method and use |
| CN108726667A (en) * | 2018-06-05 | 2018-11-02 | 佛山皖阳生物科技有限公司 | A kind of preparation method of hydrogel Microorganism-embeddcarrier carrier |
| CN108726667B (en) * | 2018-06-05 | 2021-07-16 | 上海持美生物环保科技有限公司 | Preparation method of hydrogel-embedded microorganism carrier |
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Effective date of registration: 20170607 Address after: 210000 No. 69, flying Avenue, Jiangning airport hub Economic Zone, Jiangsu, Nanjing Province Patentee after: NANJING LAM HENG HE WATER TRETMENT TECHNOLOGY CO.,LTD. Address before: 200240, 41 floor, building 1, 398 Heqing Road, Minhang District, Shanghai, Patentee before: LVCHENG MATERIAL TECHNOLOGY SHANGHAI Co.,Ltd. Effective date of registration: 20170607 Address after: 200240, 41 floor, building 1, 398 Heqing Road, Minhang District, Shanghai, Patentee after: LVCHENG MATERIAL TECHNOLOGY SHANGHAI Co.,Ltd. Address before: 200240 Dongchuan Road, Shanghai, No. 800, No. Patentee before: Shanghai Jiao Tong University |
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