CN102824409A - Preparation method of liver protection tablets - Google Patents
Preparation method of liver protection tablets Download PDFInfo
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- CN102824409A CN102824409A CN2012103594318A CN201210359431A CN102824409A CN 102824409 A CN102824409 A CN 102824409A CN 2012103594318 A CN2012103594318 A CN 2012103594318A CN 201210359431 A CN201210359431 A CN 201210359431A CN 102824409 A CN102824409 A CN 102824409A
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Abstract
The invention provides a preparation method of liver protection tablets. The liver protection tablets are prepared from the raw material medicines of 250g of radix bupleuri, 250g of herba artemisiae scopariae, 250g of radix isatidis, 300g of fructus schisandrae chinensis, 20g of pig bile powder and 128g of mung bean by microwave extraction. Through the invention, the content of schisandrin b is greatly increased.
Description
Technical field
The present invention relates to the Chinese medicine preparation technical field, be specifically related to a kind of method for preparing of HUGAN PIAN.
Background technology
HUGAN PIAN is recorded in standards of pharmacopoeia, is processed ability depressed liver-energy dispersing and QI regulating, strengthening the spleen to promote digestion as crude drug by Radix Bupleuri 250g, Herba Artemisiae Scopariae 250g, Radix Isatidis 250g, Fructus Schisandrae Chinensis 300g, Pulvis Fellis Suis 20g, Semen phaseoli radiati 128g.Has the transaminase lowering effect.Be used for chronic hepatitis and early stage liver cirrhosis etc.
In the prior art, HUGAN PIAN is not arranged as yet at the report that adopts microwave technology aspect the extraction preparation, and the method that adopts decocting to boil, technology is coarse, backward, and impurity is many, causes patient's consumption excessive, is inconvenient to take, and has had a strong impact on these article and has used clinically.
Summary of the invention
Goal of the invention:, the object of the present invention is to provide a kind of method for preparing of HUGAN PIAN in order to address the above problem.
Technical scheme: the objective of the invention is to realize through following scheme:
A kind of method for preparing of HUGAN PIAN is processed as crude drug by Radix Bupleuri 250g, Herba Artemisiae Scopariae 250g, Radix Isatidis 250g, Fructus Schisandrae Chinensis 300g, Pulvis Fellis Suis 20g, Semen phaseoli radiati 128g, and described method is made up of the following step: get Radix Bupleuri, Herba Artemisiae Scopariae, Radix Isatidis, Fructus Schisandrae Chinensis, Pulvis Fellis Suis, Semen phaseoli radiati, pulverize; 70% ethanol that adds 2L drops in the microwave extracting apparatus and carries out microwave extracting, and extraction power 400-600W extracts 2 times; Each 4-8 minute, combining extraction liquid concentrated, and is added on the D101 macroporous adsorptive resins; 50% ethanol elution is collected 5 times of amount column volume eluents, and decompression recycling ethanol concentrates and drying; Get the microwave extraction thing, add starch, 70% ethanol system granule, drying; Tabletting is processed 1000, every heavy 0.4g.
The method for preparing of above-mentioned a kind of HUGAN PIAN, said microwave extracting power 500W extracted 6 minutes at every turn.
In the prior art, every 0.5g of HUGAN PIAN, each 4,3 times on the one, the every 0.4g of HUGAN PIAN that adopts the present invention to be prepared into only needs 2 at every turn, takes 3 times in 1st, has significantly reduced dose having under the condition of more active component.This conclusion can be through following evidence.
The comparison of schisandrin B (C23H28O6) content in the HUGAN PIAN of test one, distinct methods preparation
1, instrument and reagent HUGAN PIAN of the present invention: press the preparation of embodiment 3 methods, use the 1098g crude drug, process 1000 through extracting, every heavy 0.4g, every contains the crude drug amount and is about 1g.Former HUGAN PIAN by the preparation of ministry standard method, uses the 1098g crude drug, processes 1000 through extracting, every heavy 0.5g, and every contains the crude drug amount and is about 1g.The Agilent1200 high performance liquid chromatograph; METTLER AE240 electronic analytical balance; Schisandrin B reference substance (Nat'l Pharmaceutical & Biological Products Control Institute).
2, method
Chromatographic condition and system suitability test: use octadecylsilane chemically bonded silica to be filler; Methanol-water (72:28) is a mobile phase; The detection wavelength is 254nm.Number of theoretical plate is pressed the schisandrin B peak and is calculated, and should be not less than 3000.
The preparation of reference substance solution: precision takes by weighing at 4 hours schisandrin B reference substance of 60 ℃ of drying under reduced pressure an amount of, adds methanol and processes the solution that every 1ml contains 18 μ g, promptly gets.
The preparation of need testing solution: get HUGAN PIAN of the present invention and former HUGAN PIAN, porphyrize, mixing is got 1g, and accurate the title, decide, the accurate 70% ethanol 20ml that adds, close plug, supersound process 10 minutes, centrifugal, get supernatant, promptly get.
Accurate respectively reference substance solution and each the 20 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, measure, and promptly get.
3, result
The result shows that the content of schisandrin B is the 0.65mg/ sheet in the HUGAN PIAN of the present invention; And the content of schisandrin B is the 0.18mg/ sheet in the former HUGAN PIAN, and under the situation that dose reduces, schisandrin B content improves a lot.
Above-mentioned research shows, adopts the HUGAN PIAN of the present invention's preparation, and active constituent content is higher than the HUGAN PIAN of pharmacopeia method preparation.
The specific embodiment
Below through the embodiment form; Foregoing of the present invention is remake further detailed description; But should this be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following instance, all technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Radix Bupleuri 250g, Herba Artemisiae Scopariae 250g, Radix Isatidis 250g, Fructus Schisandrae Chinensis 300g, Pulvis Fellis Suis 20g, Semen phaseoli radiati 128g, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 400W extracts 2 times; Each 4 minutes, combining extraction liquid concentrated, and is added on the D101 macroporous adsorptive resins; 50% ethanol elution is collected 5 times of amount column volume eluents, and decompression recycling ethanol concentrates and drying; Get the microwave extraction thing, add starch, 70% ethanol system granule, drying; Tabletting is processed 1000, every heavy 0.4g, and every contains the crude drug amount and is about 1g.
Through detecting, the content of schisandrin B is the 0.53mg/ sheet in the finished product.
Embodiment 2
Get Radix Bupleuri 250g, Herba Artemisiae Scopariae 250g, Radix Isatidis 250g, Fructus Schisandrae Chinensis 300g, Pulvis Fellis Suis 20g, Semen phaseoli radiati 128g, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 600W extracts 2 times; Each 8 minutes, combining extraction liquid concentrated, and is added on the D101 macroporous adsorptive resins; 50% ethanol elution is collected 5 times of amount column volume eluents, and decompression recycling ethanol concentrates and drying; Get the microwave extraction thing, add starch, 70% ethanol system granule, drying; Tabletting is processed 1000, every heavy 0.4g, and every contains the crude drug amount and is about 1g.
Through detecting, the content of schisandrin B is the 0.68mg/ sheet in the finished product.
Embodiment 3
Get Radix Bupleuri 250g, Herba Artemisiae Scopariae 250g, Radix Isatidis 250g, Fructus Schisandrae Chinensis 300g, Pulvis Fellis Suis 20g, Semen phaseoli radiati 128g, add 70% ethanol of 2L, drop in the microwave extracting apparatus and carry out microwave extracting, extraction power 500W extracts 2 times; Each 6 minutes, combining extraction liquid concentrated, and is added on the D101 macroporous adsorptive resins; 50% ethanol elution is collected 5 times of amount column volume eluents, and decompression recycling ethanol concentrates and drying; Get the microwave extraction thing, add starch, 70% ethanol system granule, drying; Tabletting is processed 1000, every heavy 0.4g, and every contains the crude drug amount and is about 1g.
Through detecting, the content of schisandrin B is the 0.65mg/ sheet in the finished product.
Claims (2)
1. the method for preparing of a HUGAN PIAN is processed as crude drug by Radix Bupleuri 250g, Herba Artemisiae Scopariae 250g, Radix Isatidis 250g, Fructus Schisandrae Chinensis 300g, Pulvis Fellis Suis 20g, Semen phaseoli radiati 128g, it is characterized in that described method is made up of the following step: get Radix Bupleuri, Herba Artemisiae Scopariae, Radix Isatidis, Fructus Schisandrae Chinensis, Pulvis Fellis Suis, Semen phaseoli radiati, pulverize; 70% ethanol that adds 2L drops in the microwave extracting apparatus and carries out microwave extracting, and extraction power 400-600W extracts 2 times; Each 4-8 minute, combining extraction liquid concentrated, and is added on the D101 macroporous adsorptive resins; 50% ethanol elution is collected 5 times of amount column volume eluents, and decompression recycling ethanol concentrates and drying; Get the microwave extraction thing, add starch, 70% ethanol system granule, drying; Tabletting is processed 1000, every heavy 0.4g.
2. according to the method for preparing of the said a kind of HUGAN PIAN of claim 1, it is characterized in that said microwave extracting power 500W, extracted 6 minutes at every turn.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103594318A CN102824409A (en) | 2012-09-24 | 2012-09-24 | Preparation method of liver protection tablets |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012103594318A CN102824409A (en) | 2012-09-24 | 2012-09-24 | Preparation method of liver protection tablets |
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| CN102824409A true CN102824409A (en) | 2012-12-19 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655743A (en) * | 2013-12-06 | 2014-03-26 | 山东中大药业有限公司 | Preparation method for Fukean tablets and application of Fukean tablets to prepare medicaments for inhibiting mice lymphoma cell BL4 cell proliferation |
| CN103655744A (en) * | 2013-12-06 | 2014-03-26 | 山东中大药业有限公司 | Preparation method for Fukean tablet and application of Fukean tablet in preparation of drug used for inhibiting proliferation of mouse lymphoma cell YAC-1 |
| CN103655742A (en) * | 2013-12-06 | 2014-03-26 | 山东中大药业有限公司 | Preparation method of fukean tablet and application of fukean tablet in preparation of drugs for inhibition of cell proliferation of mouse embryonic carcinoma cell P19 |
| CN104116822A (en) * | 2014-08-16 | 2014-10-29 | 黑龙江江恒医药科技有限公司 | Liver-protecting tablet and preparation method thereof |
-
2012
- 2012-09-24 CN CN2012103594318A patent/CN102824409A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655743A (en) * | 2013-12-06 | 2014-03-26 | 山东中大药业有限公司 | Preparation method for Fukean tablets and application of Fukean tablets to prepare medicaments for inhibiting mice lymphoma cell BL4 cell proliferation |
| CN103655744A (en) * | 2013-12-06 | 2014-03-26 | 山东中大药业有限公司 | Preparation method for Fukean tablet and application of Fukean tablet in preparation of drug used for inhibiting proliferation of mouse lymphoma cell YAC-1 |
| CN103655742A (en) * | 2013-12-06 | 2014-03-26 | 山东中大药业有限公司 | Preparation method of fukean tablet and application of fukean tablet in preparation of drugs for inhibition of cell proliferation of mouse embryonic carcinoma cell P19 |
| CN103655744B (en) * | 2013-12-06 | 2015-06-10 | 丛宁 | Preparation method for Fukean tablet and application of Fukean tablet in preparation of drug used for inhibiting proliferation of mouse lymphoma cell YAC-1 |
| CN103655742B (en) * | 2013-12-06 | 2015-07-15 | 刘梅 | Preparation method of fukean tablet and application of fukean tablet in preparation of drugs for inhibition of cell proliferation of mouse embryonic carcinoma cell P19 |
| CN103655743B (en) * | 2013-12-06 | 2015-07-29 | 刘梅 | A kind of preparation method of fukean tablet and the application in preparation suppression mouse lymphoma cell BL4 cell proliferation thereof |
| CN104116822A (en) * | 2014-08-16 | 2014-10-29 | 黑龙江江恒医药科技有限公司 | Liver-protecting tablet and preparation method thereof |
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Application publication date: 20121219 |