CN102818800A - Human bloody urine protein detection method based on chip-level test paper - Google Patents
Human bloody urine protein detection method based on chip-level test paper Download PDFInfo
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- CN102818800A CN102818800A CN2012103304974A CN201210330497A CN102818800A CN 102818800 A CN102818800 A CN 102818800A CN 2012103304974 A CN2012103304974 A CN 2012103304974A CN 201210330497 A CN201210330497 A CN 201210330497A CN 102818800 A CN102818800 A CN 102818800A
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Abstract
The invention belongs to the field of detection of biological and medical information and relates to a human bloody urine protein detection method based on chip-level test paper. The method comprises the following steps of: (1) spraying and plating metal with excellent electro-conductibility on a nano-array to prepare a nano-array chip with a Raman enhanced substrate; (2) dripping human bloody urine protein solution standard samples with different concentrations onto the nano-array chip, reacting for a certain period of time, respectively performing Raman spectral testing, finding information of a certain characteristic peak, comparing with address information and strength information, performing data statistics and constructing and forming a database; and (3) dripping a human bloody urine protein solution to be detected onto the nano-array chip with a certain structure, reacting for the certain period of time, performing the Raman spectral testing, and mutually comparing an obtained Raman spectrum with the information stored in the database to get the concentration of the human bloody urine protein solution to be detected. According to the method disclosed by the invention, the human bloody urine protein can be detected in a fast, trace and portable manner.
Description
Affiliated technical field
The invention belongs to the biomedical information detection range, relate to a kind of human blood urine protein detection method
Background technology
There is the quantitative indispensable albumen of human life activity is existed in regular meeting in the blood.The albumen of a part can filter in the glomerule of kidney and get in the urine, but can be absorbed and get back in the blood at renal tubule.Therefore, if the function of kidney is normal, the protein content little or nothing that in urine, occurs, but the albumen that when obstacle appears in kidney and urinary catheter, will spill volume becomes albuminuria.In normal person's urine trace of albumin is arranged, qualitative negative in the normal range, be designated as (-).Protein content reaches 0.15g/24h when above in the urine, claims albuminuria, the qualitative positive that occurs of routine urinalysis.Urine protein is that urine is through the muddy protein that detects in acidifying heating back.Scope Wei ≦ the 0.15g of normal person's twenty-four-hour urine albumen, routine test detects negative.As detect urine protein＞150 milligram/day, and promptly during urine protein positive, the urine albumen amount showed increased that human body is discharged is described, belong to unusual urine protein.On behalf of kidney, the urine protein lasting masculin often pathology has taken place, thus clinical can be according to the degree of how much judging ephrosis damage of urine protein positive and the effect of treatment of kidney disease.Therefore, unusual urine protein occurs, must effectively control and eliminate, the progress that prevents that sb.'s illness took a turn for the worse.
Qualitative test is the method in order to screening and guestimate urine protein content.The method of test has three kinds: test paper method, sulfosalicylic acid method and heating method of acetic acid.The sulfosalicylic acid method all is will not to have muddy or do not have to precipitate according to turbidity reaction to be decided to be feminine gender () with the heating method of acetic acid, with become turbid or precipitate be decided to be the positive (+).The sulfosalicylic acid method is easy and simple to handle, and is highly sensitive, can be widely used in generaI investigation, but it is to albuminous highly sensitive in globulin, and influence factor is more, is prone to cause false negative or false positive.The heating method of acetic acid is to the sensitivity basically identical of albumin and globulin, and influence factor is few, and accuracy is higher.
As everyone knows; Because urine protein content is trace relatively; Therefore need to concentrate in the testing process of purifying; Therefore detect now in the biomedical processing procedure of urine protein content, need checked patient to stay urine quite a certain amount of, all be undoubtedly very difficulty and painful for weak sufferer, young child and old old man like this.Under such engineering in medicine background, continue to seek a kind of fast, trace, can be portable detection means, as the detection system of urine protein content.
Summary of the invention
The purpose of this invention is to provide a kind of can be fast, trace, can detect the method for human blood urine protein portablely.Technical scheme of the present invention is following:
A kind of human blood urine protein detection method based on the chip-scale test paper comprises the following steps:
(1) the high conductive metal of spraying plating on nano-array is processed the nano-array chip with Raman enhancing substrat structure;
(2) the human blood urine protein solution standard model with variable concentrations drips on the nano-array chip, behind the reaction certain hour, carries out the Raman spectrum test respectively; Find a certain characteristic peak information; Compare address information and strength information, carry out data statistics, structure forms database;
(3) human blood urine protein drips of solution to be measured is added on the nano-array chip of certain structure; Behind the reaction certain hour; Carry out the Raman spectrum test, the Raman spectrum and the lane database canned data that obtain are compared each other, obtain the concentration of human blood urine protein solution to be measured.
As preferred implementation, described nano-array is the quantum line structure of the quantum dot particle and the one dimension of zero dimension; Described high conductive metal is one or more among Ag, Au, Pt or the Cu; Utilize magnetron sputtering technique to realize the spraying plating of high conductive metal.
The method based on the detection human blood urine protein of chip-scale test paper that the present invention proposes can be medical worker and researcher and explores micro-biomedical information new laboratory facilities are provided, and range of application is also quite extensive.Can make the detection that detects urine protein content become more convenient, more easy operating.Realize truly " chip-scale laboratory " simultaneously, and commercialization.
Embodiment
SERS (SERS): caused electromagnetism strengthens (being that physics strengthens) because surperficial local plasmon excimer is excited to be adsorbed on the organism of roughening metal surface; And the cluster on the rough surface and the molecule that adsorbs on it constitute the active site (i.e. chemistry enhancing) that Raman strengthens, and the two effect makes the Raman scattering of determinand produce great enhancement effect.Its enhancer can reach 10
3～10
7Power has found that the metal that can produce SERS has few metals such as Ag, Au, Cu and Pt, is the best with the enhancement effect of Ag, and is the most commonly used.This technology has good He the highly sensitive advantage of selectivity, and the actual detected limit can reach 10
-12The gram level.
Be elaborated in the face of technical scheme of the present invention down
(1) assemble nanometer array, spraying plating noble metal realize that Raman strengthens substrat structure
(the nano-array pattern comprises the low dimension nano-array of various patterns such as nano particle, nano wire, nanometer rods, nano belt at nano-array; Especially the quantum line structure of the quantum dot particle of zero dimension and one dimension) go up (wherein the material of nano material can be organism, inorganics or compound substance); Utilize magnetron sputtering technique to realize that the film figure layer of high conductive metal (one or more among Ag, Au, Pt, the Cu etc.) covers, and processes the nano-array chip.
(2) solution standard model to be measured titration and training program
Variable concentrations (from the lean solution to the strong solution) solution standard model to be measured is dripped on the nano-array chip, and the reaction certain hour (after 5～600s), is made the Raman spectrum test respectively.Find a certain characteristic peak information, compare address information and strength information, carry out the database data statistics.Form the database information network between differential responses time, different array structure, the different solutions concentration.
(3) testing sample is measured and the discriminating data output system
The database that step (2) trains; Can print becomes the product data handbook, can form electronic edition database information table; Also can be used as embedded code and enroll the data processing chip of hand-held Raman spectrometer, so only need to use the array chip test paper of particular nanostructure to do test, the raman spectral information that official hour appeared; Just can let information as a comparison; Be introduced in the database information and search, find corresponding database node, urine protein content what just can judge very easily.
One embodiment of the present of invention are: on the ZnO nanometer stick array, utilize magnetron sputtering technique to realize that the film figure layer of precious metals ag covers.Variable concentrations (from the lean solution to the strong solution) solution standard model to be measured is dripped on the nano-array chip, and the reaction certain hour (after 5～600s), is made the Raman spectrum test respectively.Find a certain characteristic peak information, compare address information and strength information, carry out the database data statistics.Form the database information network between differential responses time, different array structure, the different solutions concentration.) testing sample measurement and discriminating data output system. the database that step (2) trains, can print becomes the product data handbook.So only need to use the array chip test paper of particular nanostructure to do test; The raman spectral information that official hour appeared; Just can let information as a comparison; Be introduced in the database information and search, find corresponding database node, urine protein content what just can judge very easily.
Claims (4)
1. the human blood urine protein detection method based on the chip-scale test paper comprises the following steps:
(1) the high conductive metal of spraying plating on nano-array is processed the nano-array chip with Raman enhancing substrat structure;
(2) the human blood urine protein solution standard model with variable concentrations drips on the nano-array chip, behind the reaction certain hour, carries out the Raman spectrum test respectively; Find a certain characteristic peak information; Compare address information and strength information, carry out data statistics, structure forms database;
(3) human blood urine protein drips of solution to be measured is added on the nano-array chip of certain structure; Behind the reaction certain hour; Carry out the Raman spectrum test, the Raman spectrum and the lane database canned data that obtain are compared each other, obtain the concentration of human blood urine protein solution to be measured.
2. human blood urine protein detection method according to claim 1 is characterized in that, described nano-array is the quantum line structure of the quantum dot particle and the one dimension of zero dimension.
3. human blood urine protein detection method according to claim 1 and 2 is characterized in that, described high conductive metal is one or more among Ag, Au, Pt or the Cu.
4. according to any described human blood urine protein detection method of claim 1 to 3, it is characterized in that, utilize magnetron sputtering technique to realize the spraying plating of high conductive metal.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105300971A (en) * | 2015-09-21 | 2016-02-03 | 济南大学 | Preparation method of urine protein detection test paper |
| CN109406480A (en) * | 2018-09-10 | 2019-03-01 | 天津大学 | Chip-scale test paper blood sugar detecting method based on SERS technology |
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| CN1659425A (en) * | 2002-06-12 | 2005-08-24 | 英特尔公司 | Metal coated nanocrystalline silicon as an active surface enhanced raman spectroscopy (sers) substrate |
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| EP2405257A1 (en) * | 2009-03-04 | 2012-01-11 | Hasegawa, Yuki | Assaying substrate with surface-enhanced raman scattering activity |
| WO2012043028A1 (en) * | 2010-09-29 | 2012-04-05 | 株式会社日立ハイテクノロジーズ | Biopolymer optical analysis device and method |
| CN102507530A (en) * | 2011-10-26 | 2012-06-20 | 黑龙江省科学院技术物理研究所 | Method using gamma radiation for preparing nano-silver surface-enhanced Raman spectrum substrate |
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| CN1659425A (en) * | 2002-06-12 | 2005-08-24 | 英特尔公司 | Metal coated nanocrystalline silicon as an active surface enhanced raman spectroscopy (sers) substrate |
| CN101042348A (en) * | 2006-03-24 | 2007-09-26 | 中国科学院长春光学精密机械与物理研究所 | Device for nondestructively detecting carotenoid concentration in human body |
| EP2405257A1 (en) * | 2009-03-04 | 2012-01-11 | Hasegawa, Yuki | Assaying substrate with surface-enhanced raman scattering activity |
| WO2012043028A1 (en) * | 2010-09-29 | 2012-04-05 | 株式会社日立ハイテクノロジーズ | Biopolymer optical analysis device and method |
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| CN102507530A (en) * | 2011-10-26 | 2012-06-20 | 黑龙江省科学院技术物理研究所 | Method using gamma radiation for preparing nano-silver surface-enhanced Raman spectrum substrate |
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| CN105300971A (en) * | 2015-09-21 | 2016-02-03 | 济南大学 | Preparation method of urine protein detection test paper |
| CN109406480A (en) * | 2018-09-10 | 2019-03-01 | 天津大学 | Chip-scale test paper blood sugar detecting method based on SERS technology |
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