CN102818755A - Method for actual measurement of microcystis density and population size by using laser particle analyzer - Google Patents
Method for actual measurement of microcystis density and population size by using laser particle analyzer Download PDFInfo
- Publication number
- CN102818755A CN102818755A CN2012102542532A CN201210254253A CN102818755A CN 102818755 A CN102818755 A CN 102818755A CN 2012102542532 A CN2012102542532 A CN 2012102542532A CN 201210254253 A CN201210254253 A CN 201210254253A CN 102818755 A CN102818755 A CN 102818755A
- Authority
- CN
- China
- Prior art keywords
- sample
- obscurity
- density
- laser particle
- particle analyzer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a method for actual measurement of microcystis density and population size by using a laser particle analyzer. Through pretreatment of an on-site sample, rapid and simultaneous determination of microcystis density and population particle size distribution can be realized by using the laser particle analyzer. The method comprises the following concrete steps: 1, standing the sample for 12 h, collecting supernatant and supplementing distilled water with an amount equal to the amount of mixed liquid at bottom; 2, setting absorptivity of the laser particle analyzer to be 0.1, a refractive index to be 1.40 and a rotating speed of a circulating pump to be 1500 rad/min; and 3, adding clear water with a volume of V1 (600 to 700 mL) for scanning of a background value, adding the sample to be detected step by step, recording the volume V2 of the added sample and a corresponding shading degree OV when a shading degree reaches 10 to 20%, determining particle size distribution of microcystis and calculating microcystis density.
Description
Technical field
The present invention relates to a kind of method of using laser particle analyzer actual measurement Microcystis aeruginosa density and group size.Use by this method can be led to and also measured Microcystis aeruginosa density and colony's size distribution fast simultaneously.With in the past some measure the method for Microcystis aeruginosa density and group size different be; The method can be measured Microcystis aeruginosa density and group size simultaneously; Do not need broken microcapsule algae colony; Also need not spend the plenty of time with microscope measurement Microcystis aeruginosa group size, the method not only can draw Microcystis aeruginosa density and group size rapidly, and can obtain the size distribution of Microcystis aeruginosa colony.The invention belongs to the resource environment field.
Background technology
Blue-green alga bloom is generally comparatively general with a large amount of breedings, the enrichment of Microcystis aeruginosa.The Microcystis aeruginosa cell density is the basic data of wawter bloom research, prediction, control in the lake.Generally,, all be commonly used to estimate the biomass of Microcystis aeruginosa like microscopic counting, AAS, chlorophyll content to the many mensuration of research of the biomass of Microcystis aeruginosa based on cell density.Certain limitation appears in the microscopic examination meeting when research number of groups, group size, colony cell number; Though the quantity of colony is easy to obtain through counting, the cell number that each colony constitutes then since the spatial structure of colony's cell be difficult to count with blocking each other.With microscopic Microcystis aeruginosa colony the time, because sampling amount (0.1 μ L-0.1mL) is less, and often will carry out 1 times ~ 10 times dilutions, there is bigger contingency in the colony's quantity that occurs in the visual field with size, and this can cause the representative variation of evaluation result.Simultaneously, each sample all will be counted to 400 ~ 600 algae unit when carrying out colony's evaluation, and workload is also very huge.Microscopic plays a significant role for the biomass actual measurement aspect of phytoplankton classification and unicellular algae, but when the population characteristic of the Microcystis aeruginosa that occurs with colony's form is estimated, has the problem aspect operability and the accuracy.Therefore, seek a kind of easy, field and indoor sample all are suitable for, can to estimate the total cell density of Microcystis aeruginosa can be again necessity very to the method that group size and colony's size distribution are estimated.
Summary of the invention
The objective of the invention is to set up a kind of method that can lead to fast and measure Microcystis aeruginosa density and group size and size distribution simultaneously.Through adopting the assay method of patent of the present invention, can fast measuring Microcystis aeruginosa density and group size related data.
Technical scheme of the present invention is characterized in that the Microcystis aeruginosa sample that obtains in the actual lake is carried out simple pre-service, directly measures through laser particle analyzer, just can calculate the data that obtain Microcystis aeruginosa density and group size and size distribution.
Fish for 65 μ m animal plankton nets at the floating comparatively significantly water surface of Microcystis aeruginosa, preserve through taking back the laboratory after the formalin fixed.
The sample that take in the field need carry out pre-service earlier because suspension bed sediment is more.Utilize the foreign material characteristics faster such as suspension bed sediment, sample is carried out leaving standstill in 12 hours in 500mL water sample bottle than great sedimentation.After treating that silt, foreign material are deposited to water sample bottle bottom, extract the clean algae liquid in top.Replenish distilled water with the assorted liquid same amount in bottom simultaneously to guarantee the original density of algae liquid.The algae appearance of indoor pure culture can directly be tested.
Laser particle analyzer is analyzed: adopt the Britain Mastersize2000 of Ma Erwen company laser particle analyzer.Compare through microscopic examination and with reference to the absorptivity atlas, setting laser particle size analyzer absorptivity is 0.1, is 1.40 through attempting setting refractive index, and the ebullator rotating speed is that 1500rad/min guarantees that abundant mixing does not have obvious bubble again.
At first, add V
1(600-700mL) the clear water scanning background value of volume progressively adds sample again, observes the variation of obscurity simultaneously, and record adds the amount V of sample when obscurity reaches 10～20%
2Obscurity O with correspondence
V, then through measuring the size distribution that can obtain sample, through V
1, V
2And O
VCan calculate Microcystis aeruginosa density, laser particle analyzer obtains Microcystis aeruginosa group size and size distribution when remove obtaining the total obscurity of suspended particle in liquid.
Description of drawings
Fig. 1 is the relation of cell density and obscurity;
Fig. 2 is cell density and microscopic counting result contrast;
Fig. 3 is the measured result of the shared total volume percent of different-grain diameter in 4 kinds of test specimens: (a, 11; B, 5; C, 4; D, 4).
Embodiment
Case study on implementation:
(1) instrument and sample
The Britain Mastersize2000 of Ma Erwen company laser particle analyzer is adopted in test.
Microcystis aeruginosa algae kind is taken at aquatic institute of the Chinese Academy of Sciences, uses the BG-11 medium culture in the 250ml conical flask, intensity of illumination 5 ~ 50 μ Em
-2S-1,25 ℃ of cultivation temperature, Light To Dark Ratio 12:12 hour.Under different light intensity, disturbed conditions, cultivate; Obtained 3 kinds of samples that population characteristic has nothing in common with each other: naked eyes and microscopic can both clearly be differentiated: a class sample with unicellular be main; Amount to 24; The b sample is main with colony, amounts to 13, the c sample is unicellular, microcommunity with mix 9 altogether than large group.
In June, 2010, the sample position is a Taihu Lake tribute arm of lake, fishes for 63 μ m animal plankton nets at the floating comparatively significantly water surface of Microcystis aeruginosa, preserves through taking back the laboratory after the formalin fixed.Naked eyes and microscopic can both clearly be differentiated Microcystis aeruginosa and exist with the large group form, accidental unicellular and microcommunity (d, sample amounts to 13).
(2) typical curve is drawn
4 kinds of samples microscopic examination and laser particle analyzer analysis have been carried out respectively.
Microscopic examination: sample a directly obtains the algae density D through microscopic counting
m, carry out cell count after b, c, d sample disperse Microcystis aeruginosa colony through the method that adds soda boiling and boil and obtain the algae density D
m
Laser particle analyzer is analyzed: adopt the Britain Mastersize2000 of Ma Erwen company laser particle analyzer.Compare through microscopic examination and with reference to the absorptivity atlas, setting laser particle size analyzer absorptivity is 0.01, is 1.50 through attempting setting refractive index, and the ebullator rotating speed is that 1500rad/min guarantees that abundant mixing does not have obvious bubble again.
At first, add V
1(600-700mL) the clear water scanning background value of volume progressively adds sample again, observes the variation of obscurity simultaneously, and record adds the amount V of sample when obscurity reaches 10～20%
2Obscurity O with correspondence
V, the size distribution of working sample then.
Utilize the principle of suspended particle obscurity, use laser particle analyzer that the Microcystis aeruginosa cell number is surveyed.
Volume is V
2Sample obscurity O
M, can be according to the obscurity O that records after the dilution
VVolume V with dilution
1, calculate according to formula (2)
O
M=O
V(V
1+V
2)/V
2 ?(2)
With this method to 4 kinds, totally 35 samples (a, 13; B8; C, 5; D, 9) obscurity survey, survey through microscope pair cell density simultaneously, cell density and obscurity concerned drafting pattern 1.Can find out very significantly linear dependence relation of existence between the two from measured data, obtain the reduction formula (3) between cell density and the obscurity
D
c=kO
M (3)
In the formula: D
cBe the cell density through the laser particle analyzer indirect determination, k is the obscurity coefficient: obtain indoor cultivation sample k=22.98 through this experiment; Field sample k=9.03.
(3) the algae density of working sample
In order to prove the accuracy of this method, to untapped 24 samples (a, 11 in 4 kinds of samples; B, 5; C, 4; D, 4) used laser particle analyzer to measure, cell density that obtains and microscopic counting result contrast, the result sees Fig. 2.By the result of Fig. 2 visible laser particle size analyzer acquisition and the basically identical as a result of microscope acquisition.
(4) Microcystis aeruginosa group size and size distribution
When laser particle analyzer remove to obtain the total obscurity of suspended particle in liquid, can obtain the size and the grading curve of sample.The measured result of the shared total volume percent of different-grain diameter is seen Fig. 3 in 4 kinds of test specimens.
Claims (1)
- , a kind of method of using laser particle analyzer actual measurement Microcystis aeruginosa density and group size, it is characterized in that realizing as follows:The method of A, on-the-spot sample pretreatment is: sample is carried out leaving standstill in 12 hours in 500mL water sample bottle; After treating that silt, foreign material are deposited to water sample bottle bottom, extract the clean algae liquid in top; Replenish distilled water with the assorted liquid same amount in bottom simultaneously to guarantee the original density of algae liquid;B, employing laser particle analyzer: setting laser particle size analyzer absorptivity is 0.1, and setting refractive index is 1.40, and the ebullator rotating speed is 1500rad/min;The basic step that C, laser particle analyzer are measured is: add V 1Volume is the clear water scanning background value of 600-700mL, progressively adds sample again, observes the variation of obscurity simultaneously, and record adds the amount V of sample when obscurity reaches 10～20% 2Obscurity O with correspondence V, then through measuring the size distribution that can obtain sample, through V 1, V 2And O VCan calculate Microcystis aeruginosa density;Reduction formula between D, cell density and the obscurity is: D c=kO M, wherein, D cBe the cell density through the laser particle analyzer indirect determination, k is the obscurity coefficient, O MFor volume is V 2The sample obscurity; In the above parameter, indoor sample k=22.98; Field sample k=9.03;Wherein volume is V 2Sample obscurity O MCan be according to the obscurity O that records after the dilution VVolume V with dilution 1Calculate through publicity, it is characterized by O M=O V(V 1+ V 2)/V 2
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210254253.2A CN102818755B (en) | 2012-07-23 | 2012-07-23 | Method for actual measurement of microcystis density and population size by using laser particle analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210254253.2A CN102818755B (en) | 2012-07-23 | 2012-07-23 | Method for actual measurement of microcystis density and population size by using laser particle analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102818755A true CN102818755A (en) | 2012-12-12 |
| CN102818755B CN102818755B (en) | 2014-10-22 |
Family
ID=47303011
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210254253.2A Expired - Fee Related CN102818755B (en) | 2012-07-23 | 2012-07-23 | Method for actual measurement of microcystis density and population size by using laser particle analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102818755B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018207A (en) * | 2016-08-08 | 2016-10-12 | 湖南盟合投资管理有限公司 | Method for detecting particle dissolution rate |
| CN106018245A (en) * | 2016-05-20 | 2016-10-12 | 河海大学 | Method for enriching and detecting microcystis single cell and colony biomass |
| CN107976405A (en) * | 2017-12-25 | 2018-05-01 | 四川大学 | A kind of device that on-line monitoring river pollution is realized by light reaction |
| CN113984599A (en) * | 2021-10-21 | 2022-01-28 | 南通大学 | Detection method for determining size of micro plastic by using laser particle analyzer |
| CN114323162A (en) * | 2022-03-14 | 2022-04-12 | 青岛清万水技术有限公司 | Shell vegetable growth data online monitoring method and device and electronic equipment |
| CN116297027A (en) * | 2023-05-10 | 2023-06-23 | 长江三峡集团实业发展(北京)有限公司 | Method and device for measuring migration characteristics of microcystis population |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3064911B2 (en) * | 1996-06-28 | 2000-07-12 | 株式会社島津製作所 | Particle size distribution analyzer |
| CN101699286A (en) * | 2009-10-16 | 2010-04-28 | 江南大学 | Method for quantitatively detecting ampicillin by marking antibody with laterally assembled gold nanorod |
| US20110226964A1 (en) * | 2000-09-06 | 2011-09-22 | Millipore Corporation | Particle or cell analyzer and method |
| CN202175170U (en) * | 2011-06-30 | 2012-03-28 | 上海泽泉科技有限公司 | Buoy for monitoring and pre-warning algae in source area of drinking water |
-
2012
- 2012-07-23 CN CN201210254253.2A patent/CN102818755B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3064911B2 (en) * | 1996-06-28 | 2000-07-12 | 株式会社島津製作所 | Particle size distribution analyzer |
| US20110226964A1 (en) * | 2000-09-06 | 2011-09-22 | Millipore Corporation | Particle or cell analyzer and method |
| CN101699286A (en) * | 2009-10-16 | 2010-04-28 | 江南大学 | Method for quantitatively detecting ampicillin by marking antibody with laterally assembled gold nanorod |
| CN202175170U (en) * | 2011-06-30 | 2012-03-28 | 上海泽泉科技有限公司 | Buoy for monitoring and pre-warning algae in source area of drinking water |
Non-Patent Citations (2)
| Title |
|---|
| 李红晖 等: "氧化铁纳米颗粒固定化溶藻菌的除藻作用", 《东南大学学报（自然科学版）》, vol. 36, no. 6, 30 November 2006 (2006-11-30), pages 986 - 1000 * |
| 陈君 等: "江苏灌河口海域现代沉积特征研究", 《资源调查与环境》, vol. 27, no. 1, 31 December 2006 (2006-12-31), pages 39 - 45 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018245A (en) * | 2016-05-20 | 2016-10-12 | 河海大学 | Method for enriching and detecting microcystis single cell and colony biomass |
| CN106018207A (en) * | 2016-08-08 | 2016-10-12 | 湖南盟合投资管理有限公司 | Method for detecting particle dissolution rate |
| CN107976405A (en) * | 2017-12-25 | 2018-05-01 | 四川大学 | A kind of device that on-line monitoring river pollution is realized by light reaction |
| CN113984599A (en) * | 2021-10-21 | 2022-01-28 | 南通大学 | Detection method for determining size of micro plastic by using laser particle analyzer |
| CN114323162A (en) * | 2022-03-14 | 2022-04-12 | 青岛清万水技术有限公司 | Shell vegetable growth data online monitoring method and device and electronic equipment |
| CN114323162B (en) * | 2022-03-14 | 2022-06-28 | 青岛清万水技术有限公司 | Method and device for on-line monitoring of shell vegetable growth data and electronic equipment |
| CN116297027A (en) * | 2023-05-10 | 2023-06-23 | 长江三峡集团实业发展(北京)有限公司 | Method and device for measuring migration characteristics of microcystis population |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102818755B (en) | 2014-10-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102818755B (en) | Method for actual measurement of microcystis density and population size by using laser particle analyzer | |
| Giering et al. | Sinking organic particles in the ocean—flux estimates from in situ optical devices | |
| Graff et al. | Analytical phytoplankton carbon measurements spanning diverse ecosystems | |
| Hötzel et al. | A phytoplankton methods manual for Australian freshwaters | |
| Vadrucci et al. | Biovolume determination of phytoplankton guilds in transitional water ecosystems of Mediterranean Ecoregion | |
| Li et al. | Analysis of cell concentration, volume concentration, and colony size of Microcystis via laser particle analyzer | |
| Hrycik et al. | Comparison of FlowCAM and microscope biovolume measurements for a diverse freshwater phytoplankton community | |
| Foladori et al. | Use of silica microspheres having refractive index similar to bacteria for conversion of flow cytometric forward light scatter into biovolume | |
| Moncheva et al. | Manual for phytoplankton sampling and analysis in the black sea | |
| Yacobi et al. | Carbon: chlorophyll a ratio, assimilation numbers and turnover times of Lake Kinneret phytoplankton | |
| Prieto et al. | Scales and processes in the aggregation of diatom blooms: high time resolution and wide size range records in a mesocosm study | |
| Walsby et al. | Sinking velocities of phytoplankton measured on a stable density gradient by laser scanning | |
| Dromph et al. | Sources of uncertainty in assessment of marine phytoplankton communities | |
| Barone et al. | Particle distributions and dynamics in the euphotic zone of the N orth P acific S ubtropical G yre | |
| Serra et al. | Evaluation of laser in situ scattering instrument for measuring concentration of phytoplankton, purple sulfur bacteria, and suspended inorganic sediments in lakes | |
| Merz et al. | Underwater dual-magnification imaging for automated lake plankton monitoring | |
| Brunelle et al. | Variability of phytoplankton light absorption in Canadian Arctic seas | |
| Priyadarshi et al. | Micro-scale patchiness enhances trophic transfer efficiency and potential plankton biodiversity | |
| Hitchcock et al. | Zooplankton responses to freshwater inflows and organic-matter pulses in a wave-dominated estuary | |
| Whyte et al. | Tracking changes to a microplankton community in a North Atlantic sea loch using the microplankton index PI (mp) | |
| Sacchi et al. | Leukocyte differential counts and morphology from twelve European lizards | |
| Davies et al. | The hidden influence of large particles on ocean colour | |
| Wang et al. | The distributions of autumn picoplankton in relation to environmental factors in the reservoirs along the Wujiang River in Guizhou Province, SW China | |
| Mahlmann et al. | Rapid determination of the dry weight of single, living cyanobacterial cells using the Mach-Zehnder double-beam interference microscope | |
| Rzadkowolski et al. | Using laser scattering to identify diatoms and conduct aggregation experiments |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141022 Termination date: 20210723 |