CN102805106B - A kind of composition pesticide containing bacillus thuringiensis,Bt and metaflumizone - Google Patents
A kind of composition pesticide containing bacillus thuringiensis,Bt and metaflumizone Download PDFInfo
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- CN102805106B CN102805106B CN201210253278.0A CN201210253278A CN102805106B CN 102805106 B CN102805106 B CN 102805106B CN 201210253278 A CN201210253278 A CN 201210253278A CN 102805106 B CN102805106 B CN 102805106B
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Abstract
The invention discloses a kind of composition pesticide containing bacillus thuringiensis,Bt and metaflumizone, comprise usual auxiliaries, bacillus thuringiensis,Bt and metaflumizone, the ratio of weight and number of bacillus thuringiensis,Bt and metaflumizone is 20<b>:</bGreatT. GreaT.GT1-1<b>:</b>20, and bacillus thuringiensis,Bt and the percentage by weight of metaflumizone weight summation in composition pesticide are 1-60%.Described auxiliary agent is one or more in solvent, emulsifier, wetting agent, stabilizing agent, dispersant, thickener, pH adjusting agent, defoamer, antifreezing agent, filler.This composition pesticide for preventing and treating crops Lepidoptera class pest.This composition pesticide is all better than single dose in quick-acting and lasting effect, and reduces the using dosage of agricultural chemicals, reduces drug cost, is conducive to the comprehensive regulation of environmental protection and pest.
Description
Technical field
The composition pesticide that the present invention relates to is with bacillus thuringiensis,Bt and metaflumizone for active ingredient, for preventing and treating crops Lepidoptera class pest.
Background technology
The resistance problem of pest is global problems, is the emphasis problem that agricultural science and technology worker pays close attention to always.Along with the continuity year in year out of Chemical Control of Harmful Insects, the increase of Pesticide use amount and not scientifical use agricultural chemicals, pest resistance is day by day serious, and the pest species producing resistance is on the increase.Particularly to the Lepidoptera class pest that agricultural production is caused harm serious, resistance level is higher, and resistance development speed is relatively very fast.Meanwhile, the use agricultural chemicals of high strength, causes Practice for Pesticide Residue in Agricultural Products to exceed standard, environmental pollution and the increase of peasant's drug cost etc., is unfavorable for agricultural sustainable development.Therefore, the pesticide of efficient, the low toxicity of research and development, environmental protection has positive effect to agricultural sustainable development.
Bacillus thuringiensis,Bt (Bacillusthuringiensis is called for short Bt) is the biological insecticides that current production rate is maximum, use is the widest.Its main active is one or several insecticidal crystal proteins (insecticidalcrystalproteins, ICPs), also known as delta-endotoxin, to Lepidoptera, coleoptera, diptera, Hymenoptera, the insects such as Homoptera, and animals and plants nematode, the arthropodss such as acarian have specific cytotoxicity, and to nontarget organism safety therefore, Bt insecticide has single-minded, efficient and kind more than 100 has been reached to the current bacillus thuringiensis,Bt commercial preparation of the advantages such as person poultry safety, be most widely used in the world, consumption is maximum, the microorganism insecticide that effect is best, thus extremely people pay close attention to.But, commodity Bt preparation production control in also demonstrate some limitation, as poor in quick-acting, insensitive on high instar larvae, the field lasting period is short and recombinant strain genetic character instability etc. all become the restraining factors affecting Bt and successfully promote the use of further.
Metaflumizone (metaflumizone) is a kind of insecticide of brand-new mechanism of action, and this medicine is mainly entered by pests and in its body, stomach toxicity occurs and kill off the insect pests, and action of contace poison is less, without systemic action.This medicine has good control efficiency for the target pest in each length of time, larva, after insect's food-taking, this medicine enters polypide, the sodium-ion channel on insect neuron axon film is blocked by the mechanism of action of uniqueness, make sodium ion not by axolemma, and then suppress nerve impulse to make excessive the loosening of polypide, paralysis, after several hours, namely insect stops taking food, dead in 1-3d, effectively can prevent and treat larva, the adult of each Species of Lepidopterous Insect Pests and some coleoptera, can also be used for preventing and treating the insects such as ant, termite, fly class, cicada dung beetle.With the compound of chrysanthemum ester class or other kinds without cross resistance.To mammal impassivity toxicity, low to the acute toxicity of birds, to the low danger of honeybee, owing to can promptly be hydrolyzed and photodissociation in water, aquatile is endangered without reality.
The product price of metaflumizone is higher, and during single use, drug cost is high, limits it to a certain extent and applies, and long-term single use will cause pest resistance degree constantly to increase.There is the shortcomings such as quick-acting difference, insensitive to high instar larvae, the lasting period is short in bacillus thuringiensis,Bt when single use.
The mixture of different pesticide species is the common methods of preventing and treating agriculture resistant insect.Screened by formulation of pesticide, filter out rational formula, can effectively improve actual control efficiency, reduce dosage, reduce costs, delay the generation of pest resistance to insecticide, extending the service life of medicament, is the important means of integrated pest management.The present inventor, on the basis of a large amount of indoor bioassay and field medicine effect test, finds that bacillus thuringiensis,Bt and metaflumizone mixture have obvious synergistic effect to Lepidoptera class pest, completes the present invention after further study.
The mixture control Lepidoptera class pest of bacillus thuringiensis,Bt and metaflumizone there is no report and application at present.
Summary of the invention
The technical problem that the present invention solves be to provide a kind of efficient, low toxicity and the lasting period long, effectively can prevent and treat crops Lepidoptera class pest, be conducive to the composition pesticide containing bacillus thuringiensis,Bt and metaflumizone of integrated pest management.
For solving the problem, the technical solution adopted in the present invention is: a kind of composition pesticide containing bacillus thuringiensis,Bt and metaflumizone, comprise usual auxiliaries, bacillus thuringiensis,Bt and metaflumizone, the ratio of weight and number of bacillus thuringiensis,Bt and metaflumizone is 20
:1-1
:20, bacillus thuringiensis,Bt and the percentage by weight of metaflumizone weight summation in composition pesticide are 1-60%.
Preferably, the ratio of weight and number of bacillus thuringiensis,Bt and metaflumizone is 10
:1-1
:1.
Described composition pesticide according to well known to a person skilled in the art method, can be mixed with the application forms of the applicable agricultural use such as suspending agent or wetting powder.
Described composition pesticide is also containing the usual auxiliaries of preparation needed for pesticidal preparations, usual auxiliaries is the mixing of one or more in solvent, emulsifier, wetting agent, stabilizing agent, dispersant, thickener, pH adjusting agent, defoamer, antifreezing agent, packing agent etc., be known substance, various auxiliary agents conventional in pesticidal preparations, can change to some extent according to different situations, be not particularly limited.
Described composition pesticide, for preventing and treating crops Lepidoptera class pest.
Composition pesticide provided by the invention, usually adopts the method for spraying to use, also can adopt other operation techniques agriculturally applied as required.
Composition pesticide provided by the invention has following beneficial effect:
1, composition pesticide provided by the invention, shows obvious synergistic effect in certain ratio range, and the control efficiency of composition is significantly improved than single dose, reduces the using dosage of agricultural chemicals, decreases the impact of agricultural chemicals on environment, reduces drug cost.
2, the active ingredient mechanism of action of composition pesticide provided by the invention is different, and the application of composition can delay or overcome the generation of disease drug resistance, extends the service life of medicament, is conducive to the comprehensive regulation of insect.
Technology origin of the present invention: for understanding bacillus thuringiensis,Bt and metaflumizone mixture further to the co-toxicity of Lepidoptera class pest, the present inventor has carried out Toxicity Determination test to bacillus thuringiensis,Bt and metaflumizone mixture.
The eggplant diamondback moth larvae that field gathers is put into off-the-shelf Chinese cabbage seedling, after larvae pupation, pupa is collected in the culture dish being lined with moisturizing filter paper, put into cage of laying eggs to sprout wings by it, then the Chinese cabbage seedling of whole dish being put into cage of laying eggs allows diamondback moth larvae lay eggs, often coil Chinese cabbage seedling and fall ovum amount when reaching about 500, take out Chinese cabbage seedling dish, be placed on climatic cabinate [temperature: 24-26 DEG C; Relative moisture 70%-80%; Illumination: 14 (L): 10 (D)] in allow egg hatching, raise to 3 instar larvaes.
Test medicine adopts 50000IU/ milligram Bacillus thuringiensis, and the former medicine of 96% metaflumizone, is mixed with the preparation of desired concn by research and development centre of Guangdong Zhongxun Agricultural Technology Co., Ltd..
The malicious blade method of folder is adopted to measure: to beat with the card punch of diameter 1cm and get leaf butterfly, put into culture dish, moisturizing.With capillary dropper from low concentration, every leaf butterfly drop 1ul liquid, involutory the making of leaf butterfly scribbling gelatinized corn starch after solvent volatilization with another sheet presss from both sides malicious leaf butterfly, makes in the complete hole being put in 12 hole tissue culturing plates.Often process and repeat for 4 times, often repeat to be no less than 12 malicious leaf butterflies of folder, and establish not containing the corresponding organic solvent of medicament process in contrast.Connect 1 examination worm in each hole of tissue culturing plate, be placed in normal condition and cultivate.After meeting worm 2-4h, after examination worm has taken food pastille leaf butterfly, in cultivation plate hole, add clean feeds continue to raise to investigation, eliminated the examination worm not eaten a complete leaf butterfly.Each dosage process examination borer population 80.After process, 48h investigation examination worm death condition, records total borer population and dead borer population.
According to survey data, calculate the corrected mortality of each process.And calculate co-toxicity coefficient (the CTC value of mixture in abundant method according to grandson.If) contrast lethality < 5%, do not correct; Contrast lethality, between 5%-20%, corrects; Contrast lethality > 20%, test need be reformed.Be independent variable x according to the logarithm value of drug concentration (mg/L), with the probit value of corrected mortality for dependent variable y, set up virulence regression equation formula respectively, adopt DPS software to calculate the LC of single dose and each proportioning mixture
50and mixture co-toxicity coefficient, compare synergy situation.
According to the NY/T11547.7-2006 insecticide combination effect criteria for classifying: co-toxicity coefficient (CTC value) >=120 shows as synergistic effect; Co-toxicity coefficient (CTC value)≤120 shows as antagonism; 80 < co-toxicity coefficients (CTC value) < 80 shows as summation action.As can be seen from result of the test table 1, bacillus thuringiensis,Bt and metaflumizone, 20
:1-1
:20(by weight) scope in, co-toxicity coefficient is all greater than 120, shows as synergistic effect to eggplant diamond-back moth.Bacillus thuringiensis,Bt and metaflumizone mixture have reasonability and feasibility.
Table 1, bacillus thuringiensis,Bt and metaflumizone and different ratio are to eggplant diamond-back moth toxicity test result.
Embodiment
Be described in further detail content of the present invention below in conjunction with embodiment, content mentioned in embodiment is not limitation of the invention, and material prescription is selected to suit measures to local conditions and on result without substantial impact.In these embodiments, unless otherwise indicated, all percentage is all weight percentage.
embodiment 1: 20% bacillus thuringiensis,Bt metaflumizone suspending agent.
By bacillus thuringiensis,Bt 2%, metaflumizone 18%, mineral oil (solvent) 10%, naphthalene (bleeding agent) 2%, methyl naphthalene sulfonate formaldehyde condensate (dispersant) 5%, agriculture breast 0203B(surfactant) 6%, white carbon (filler) 4%, aluminium-magnesium silicate (stabilizing agent) 0.5%, ethylene glycol (antifreezing agent) 3%, tributyl phosphate (defoamer) 1.5%, deionized water complements to 100%.
Above-mentioned formula is pulverized in proportion in advance, then adds in sand mill and grind, the obtained 20% bacillus thuringiensis,Bt metaflumizone suspending agent of allotment after high shear mixing.
embodiment 2: 20% bacillus thuringiensis,Bt metaflumizone wetting powder.
By bacillus thuringiensis,Bt 5%, metaflumizone 15%, fatty alcohol-polyoxyethylene ether (bleeding agent) 2%, lauryl sodium sulfate (surfactant) 2%, white carbon (filler) 8%, sodium lignin sulfonate (dispersant) 5%, bentonite (filler) complements to 100%.
Mix entering in blender after above-mentioned formula in proportion coarse crushing, more namely obtain 20% bacillus thuringiensis,Bt metaflumizone wetting powder after air-flow crushing.
embodiment 3: the test of pesticide effectiveness of control wild cabbage diamond-back moth.
Adopt 20% bacillus thuringiensis,Bt metaflumizone suspending agent (embodiment 1) control wild cabbage diamond-back moth, contain in diamondback moth larvae and send out phase dispenser, be watered even spraying.Adopt 32000IU/ milligram bacillus thuringiensis,Bt wetting powder and 24% metaflumizone suspending agent medicament in contrast.4 chemicals treatment are established in test altogether, and each process repeats for 4 times, and before dispenser, investigate insect population radix, 3 days, 5 days, 10 days investigation prevention effects after dispenser, result of the test is as shown in table 2.
Table 2, control wild cabbage diamond-back moth field control effectiveness test result.
embodiment 4: the test of pesticide effectiveness of control paddy rice rice leaf roller.
Adopt 20% bacillus thuringiensis,Bt metaflumizone suspending agent (embodiment 1) control paddy rice rice leaf roller, in the dispenser of egg hatching peak period, be watered even spraying.Adopt 32000IU/ milligram bacillus thuringiensis,Bt wetting powder and 24% metaflumizone suspending agent medicament in contrast.4 chemicals treatment are established in test altogether, and each process repeats for 4 times, and before dispenser, investigate insect population radix, 1 day, 7 days, 14 days investigation prevention effects after dispenser, result of the test is as shown in table 3.
Table 3, control paddy rice rice leaf roller field control effectiveness test result.
embodiment 5: the test of pesticide effectiveness of control smaller apple leafrol-ler.
Adopt 20% bacillus thuringiensis,Bt metaflumizone wetting powder (embodiment 2) control smaller apple leafrol-ler, in the dispenser of low instar larvae occurrence in peak period, be watered even spraying.Adopt 32000IU/ milligram bacillus thuringiensis,Bt wetting powder and 24% metaflumizone suspending agent medicament in contrast.4 chemicals treatment are established in test altogether, and each process repeats for 4 times, and before dispenser, investigate insect population radix, 1 day, 7 days, 14 days investigation prevention effects after dispenser, result of the test is as shown in table 3.
Table 4, control smaller apple leafrol-ler field control effectiveness test result.
As can be seen from embodiment 3-5, bacillus thuringiensis,Bt of the present invention and the control efficiency of metaflumizone mixture composition to Lepidoptera class pests such as wild cabbage diamond-back moth, paddy rice rice leaf roller, smaller apple leafrol-ler are all better than contrasting single dose in quick-acting and lasting effect, and dosage reduces than single dose, after bacillus thuringiensis,Bt and metaflumizone mixture are described, synergistic effect is obvious.
Claims (1)
1. contain a composition pesticide for bacillus thuringiensis,Bt and metaflumizone, it is characterized in that weight ratio is: bacillus thuringiensis,Bt 2%, metaflumizone 18%, mineral oil 10%, naphthalene 2%, methyl naphthalene sulfonate formaldehyde condensate 5%, agriculture breast 0203B6%, white carbon 4%, aluminium-magnesium silicate 0.5%, ethylene glycol 3%, tributyl phosphate 1.5%, deionized water complements to 100%;
Above-mentioned formula is pulverized in proportion in advance, then adds in sand mill and grind, the obtained 20% bacillus thuringiensis,Bt metaflumizone suspending agent of allotment after high shear mixing.
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| CN104255759B (en) * | 2014-09-19 | 2016-03-30 | 青岛瀚生生物科技股份有限公司 | Containing the Pesticidal combination of matrine and metaflumizone |
| CN106070333A (en) * | 2016-08-31 | 2016-11-09 | 湖南泽丰农化有限公司 | A kind of viral insecticide containing metaflumizone |
| CN114246190B (en) * | 2022-01-19 | 2023-07-18 | 安徽农业大学 | Insecticidal composition containing metaflumizone and Etabacillus brevis and application thereof |
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| Bt工程菌WG-001与化学杀虫剂复配最佳配方筛选及田间防效研究;张聪冲等;《应用昆虫学报》;20111231;第48卷(第2期);313-318 * |
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