CN102796664A - Human body ligament tissue engineering bioreactor - Google Patents
Human body ligament tissue engineering bioreactor Download PDFInfo
- Publication number
- CN102796664A CN102796664A CN2011101391460A CN201110139146A CN102796664A CN 102796664 A CN102796664 A CN 102796664A CN 2011101391460 A CN2011101391460 A CN 2011101391460A CN 201110139146 A CN201110139146 A CN 201110139146A CN 102796664 A CN102796664 A CN 102796664A
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- CN
- China
- Prior art keywords
- lower fixed
- drive motor
- fixed disk
- culturing room
- human body
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Abstract
The invention relates to a human body ligament tissue engineering bioreactor. The invention is characterized in that three upright columns (7) are symmetrically distributed and fixedly connected between a top tray (11) and a lower fixed tray (4); the lower fixed tray (4) is symmetrically distributed with six reactor culture chambers (17) in a circumferential mode; a linear drive motor (9) is installed on an upper fixed tray (8), and the output shaft of the linear drive motor (9) is connected to the center of a mobile tray (10); a linear displacement sensor (12) is installed on the upper fixed tray (8); a tensile/compressive stress and torque sensor (14) is connected with an anchor rod (16) through a coupling (15); a bracket is installed between the anchor rod (16) and an anchor bed (19); and a rotary drive motor (6) is installed at the center of the lower fixed tray (4). The invention can perform tension/compression and two-way torsional multidimensional stress loading, can automatically detect the loosening condition of the bracket and perform bracket loosening on-line compensation, and has the characteristics of simple and compact structure and high use convenience.
Description
Technical field:
The present invention relates to a kind of human body ligament tissue engineering bio-reactor, belong to organizational project bioreactor construction design field.
Background technology:
Bio-reactor is to be used for the key equipment that human body ligament etc. is organized vitro culture in the Tissue Engineering Study.In ligament vitro culture process, the mechanical stress of appropriateness stimulates the formation of tissue and the functionalization of tissue is played a driving role, characteristic that can the betterment works tissue.But intensity size, effect frequency, persistence or characteristics such as discontinuity and working cycle for required stress are still known little about it.
At present, the bio-reactor of the type mainly partly is made up of reactor drum culturing room, straight line and rotational motion mechanism, nutrient solution circulating control device, parameter measuring apparatus, temperature-control device etc.With the three-dimensional rack clamping of having planted stem cell in the reactor drum culturing room up and down on two anchor axles; Running gear makes support produce straight line stretchings/compression and circumference torsional displacement with the anchor axle, thus to support and on the cell or tissue generation draw/press and the torsional stress stimulation.Translational motion is realized by stepper-motor, gear pair and lead screw pair of nut, rotatablely moves and is realized by another stepper-motor and gear pair.
The rotating moving part of existing these organizational project bio-reactors has increased straight-line inertia along with stretching anchor axle is done straight-line displacement together, thereby the speed control of straight-line displacement is had a negative impact; The transmission efficiency of the transmission chain of realization straight-line displacement is lower, and is prone to because of insufficient lubrication produces vibration and noise, and possible cell growth is unfavorable; Many culturing room structure arranged side by side needs bigger fitting operation space, and is unfavorable for the cell growing state in the visual inspection culturing room; When adopting a plurality of culturing room to carry out cell or tissue simultaneously when cultivating, cause owing to the relax level of support is inconsistent to draw/when stress is inconsistent, be not easy to adjust; Lack the necessary straight-line displacement and the detection and the display unit of torsional displacement.
Summary of the invention:
In order to overcome the deficiency of prior art; The object of the present invention is to provide a kind of human body ligament tissue engineering bio-reactor; Can stretch/compress and the loading of bidirectional torsion multidimensional stress; Can detect the support relaxation cases automatically and can carry out the lax online compensation of support, have characteristics simple in structure, compact, easy to use.
The present invention realizes above-mentioned purpose through following technical scheme.
In a kind of structure of human body ligament tissue engineering bio-reactor, be provided with base, chassis, union lever, lower fixed disk, driving toothed gear, rotary drive motor, column, go up shaft collar, linear drive motor, displacement disc, take over a business, linear displacement transducer, driving stem, draw/stress and torque transducer, shaft coupling, anchor pole, reactor drum culturing room, support, anchoring part, rotating disk, follower gear, needle thrust bearing; Take over a business and lower fixed disk between, be symmetrically distributed and be fixedly connected with three root posts; On lower fixed disk, be the circumference symmetrical distribution and be provided with six reactor drum culturing room; On column, take over a business and lower fixed disk between, be disposed with displacement disc and last shaft collar; Be connected through rolling bearing between displacement disc and the column; Last shaft collar is fixedly connected on the column; Linear drive motor is fixedly mounted on the shaft collar, its output shaft be fixed on through being threaded displacement disc in the heart; Linear displacement transducer is installed on the shaft collar, and its measuring sonde is connected on the displacement disc; The top of driving stem is provided with screw thread, is fixed on the displacement disc through being threaded; The bottom of driving stem is equipped with and draws/stress and torque transducer, and is connected with anchor pole through shaft coupling; The bottom that reactor drum culturing room is installed on the rotating disk, anchoring part is installed in reactor drum culturing room; The lower end of anchor pole is inserted in the reactor drum culturing room; The support of having planted cell is installed between anchor pole and the anchoring part; Rotary drive motor be installed in lower fixed disk in the heart, its output shaft is passed down through lower fixed disk, driving toothed gear is installed on the output shaft of rotary drive motor; The hub of rotating disk is passed down through lower fixed disk, and follower gear is installed on the hub of rotating disk; Driving toothed gear is meshed with follower gear; The chassis is installed on the base through needle thrust bearing.
The present invention has following beneficial effect compared with prior art:
1. the present invention can provide multidimensional stress such as stretching/compression and bidirectional torsion to load, and can realize the translational motion and the bidirectional torsion motion of independent control and interlock control, can realize automatic measurement, control and the demonstration of stress, displacement, and is simple in structure, easy to use.
2. 6 reactor drum culturing room of the present invention are circumference and are symmetrically distributed, reactor monolith can ± 180 ° of transpositions, be convenient to install and observe each culturing room.
3. the present invention can detect each support relaxation cases automatically and independently carry out the lax online compensation of support.
Description of drawings
Fig. 1 is an overall structure synoptic diagram of the present invention.
Fig. 2 is the distribution schematic diagram of culturing room of the present invention position.
In the drawings: 1. base, 2. the chassis, 3. union lever, 4. lower fixed disk, 5. driving toothed gear, 6. rotary drive motor, 7. column, 8. go up shaft collar, 9. linear drive motor, 10. displacement disc, 11. is taken over a business, 12. linear displacement transducers, 13. driving stems, 14. draw/stress and torque transducer, 15. shaft couplings, 16. anchor poles, 17. reactor drum culturing room, 18. supports, 19. anchoring parts, 20. rotating disks, 21. follower gears, 22. needle thrust bearings.
Embodiment:
A kind of human body ligament tissue of the present invention engineering bio-reactor by base 1, chassis 2, union lever 3, lower fixed disk 4, driving toothed gear 5, rotary drive motor 6, column 7, go up shaft collar 8, linear drive motor 9, displacement disc 10, take over a business 11, linear displacement transducer 12, driving stem 13, draw/stress and torque transducer 14, shaft coupling 15, anchor pole 16, reactor drum culturing room 17, support 18, anchoring part 19, rotating disk 20, follower gear 21, needle thrust bearing 22 form; Take over a business 11 and lower fixed disk 4 between, be symmetrically distributed and be fixedly connected with three root posts 7; On lower fixed disk 4, be the circumference symmetrical distribution and be provided with six reactor drum culturing room 17; On column 7, take over a business 11 and lower fixed disk 4 between, be disposed with displacement disc 10 and last shaft collar 8; Be connected through rolling bearing between displacement disc 10 and the column 7; Last shaft collar 8 is fixedly connected on the column 7; Linear drive motor 9 is fixedly mounted on the shaft collar 8, its output shaft be fixed on through being threaded displacement disc 10 in the heart; Displacement disc 10 can be made translational motion up and down along column 7; Linear displacement transducer 12 is installed on the shaft collar 8, and its measuring sonde is connected on the displacement disc 10; Thereby obtain the stretching/compression displacement amount of support 18 through the size of measuring displacement disc 10 straight-line displacements; The top of driving stem 13 is provided with screw thread, is fixed on through being threaded on the displacement disc 10, and the bottom of driving stem 13 is equipped with and draws/stress and torque transducer 14, and is connected with anchor pole 16 through shaft coupling 15; Draw/stress and torque transducer 14 be integrated sensor, can record the stretching/stress under compression of support 18 and the size of torque; The bottom that columniform reactor drum culturing room 17 is installed on the rotating disk 20, anchoring part 19 is installed in reactor drum culturing room 17 of adopting that transparent engineering plastics process; The lower end of anchor pole 16 is inserted in the reactor drum culturing room 17; The support 18 of having planted cell is installed between anchor pole 16 and the anchoring part 19; Rotary drive motor 6 be installed in lower fixed disk 4 in the heart, its output shaft is passed down through lower fixed disk 4, driving toothed gear 5 is installed on the output shaft of rotary drive motor 6; The hub of rotating disk 20 is passed down through lower fixed disk 4, and follower gear 21 is installed on the hub of rotating disk 20; Driving toothed gear 5 is meshed with follower gear 21; Chassis 2 is installed on the base 1 through needle thrust bearing 22, makes bio-reactor can realize ± 180 ° transposition.
Claims (3)
1. human body ligament tissue engineering bio-reactor; By base (1), union lever (3), lower fixed disk (4), column (7), go up shaft collar (8), take over a business (11), driving stem (13), shaft coupling (15), anchor pole (16), reactor drum culturing room (17), support (18), anchoring part (19) form, and it is characterized in that in its structure, being provided with chassis (2), driving toothed gear (5), rotary drive motor (6), linear drive motor (9), displacement disc (10), linear displacement transducer (12), draw/stress and torque transducer (14), rotating disk (20), follower gear (21), needle thrust bearing (22); Taking over a business between (11) and the lower fixed disk (4), be symmetrically distributed and be fixedly connected with three root posts (7); On lower fixed disk (4), be the circumference symmetrical distribution and be provided with six reactor drum culturing room (17); On column (7), taking over a business between (11) and the lower fixed disk (4), be disposed with displacement disc (10) and last shaft collar (8); Last shaft collar (8) is fixedly connected on the column (7); Linear drive motor (9) is fixedly mounted on the shaft collar (8), its output shaft be fixed on through being threaded displacement disc (10) in the heart; Linear displacement transducer (12) is installed on the shaft collar (8), and its measuring sonde is connected on the displacement disc (10); The top of driving stem (13) is provided with screw thread, is fixed on through being threaded on the displacement disc (10), and the bottom of driving stem (13) is equipped with and draws/stress and torque transducer (14), and is connected with anchor pole (16) through shaft coupling (15); Reactor drum culturing room (17) is installed in the bottom that rotating disk (20) is gone up, anchoring part (19) is installed in reactor drum culturing room (17); The lower end of anchor pole (16) is inserted in the reactor drum culturing room (17); Support (18) is installed between anchor pole (16) and the anchoring part (19); Rotary drive motor (6) be installed in lower fixed disk (4) in the heart, its output shaft is passed down through lower fixed disk (4), driving toothed gear (5) is installed on the output shaft of rotary drive motor (6); The hub of rotating disk (20) is passed down through lower fixed disk (4), and follower gear (21) is installed on the hub of rotating disk (20); Driving toothed gear (5) is meshed with follower gear (21).
2. a kind of human body ligament tissue engineering bio-reactor according to claim 1 is characterized in that being connected through rolling bearing between displacement disc (10) and the column (7).
3. a kind of human body ligament tissue engineering bio-reactor according to claim 2 is characterized in that chassis (2) is installed on the base (1) through needle thrust bearing (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101391460A CN102796664A (en) | 2011-05-27 | 2011-05-27 | Human body ligament tissue engineering bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101391460A CN102796664A (en) | 2011-05-27 | 2011-05-27 | Human body ligament tissue engineering bioreactor |
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| Publication Number | Publication Date |
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| CN102796664A true CN102796664A (en) | 2012-11-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101391460A Pending CN102796664A (en) | 2011-05-27 | 2011-05-27 | Human body ligament tissue engineering bioreactor |
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| CN (1) | CN102796664A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104046564A (en) * | 2014-06-06 | 2014-09-17 | 上海交通大学 | Physiological environment-imitating mechanical stimulation type biological reactor system |
| WO2014141136A1 (en) | 2013-03-13 | 2014-09-18 | Association For The Advancement Of Tissue Engineering And Cell Based Technologies And Therapies - A4Tec | Rotational dual chamber bioreactor: methods and uses thereof |
| CN110484426A (en) * | 2019-09-16 | 2019-11-22 | 常州市第一人民医院 | A kind of power stimulation loading device and its working method |
| CN111748472A (en) * | 2020-07-10 | 2020-10-09 | 深圳市第二人民医院(深圳市转化医学研究院) | Tendon tissue bionic culture ware |
| CN115369037A (en) * | 2022-10-24 | 2022-11-22 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Full circulation device, method and loading system for spinal culture solution |
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| CN101245314A (en) * | 2007-12-28 | 2008-08-20 | 北京航空航天大学 | Arterial vessel tissue engineering reactor simulating physiological pulsating flow surrounding |
| CN101250501A (en) * | 2008-04-11 | 2008-08-27 | 北京航空航天大学 | Method for cultivating cartilage by simulating knee joint physical environment and biological reactor |
| CN101372664A (en) * | 2008-05-16 | 2009-02-25 | 北京航空航天大学 | Tissue engineering reactor having tissue cultures tension-compression and rotation functions |
| CN201704334U (en) * | 2010-05-27 | 2011-01-12 | 长江大学 | Looseness compensation device for tissue engineering bioreactor support |
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2011
- 2011-05-27 CN CN2011101391460A patent/CN102796664A/en active Pending
Patent Citations (4)
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| CN101245314A (en) * | 2007-12-28 | 2008-08-20 | 北京航空航天大学 | Arterial vessel tissue engineering reactor simulating physiological pulsating flow surrounding |
| CN101250501A (en) * | 2008-04-11 | 2008-08-27 | 北京航空航天大学 | Method for cultivating cartilage by simulating knee joint physical environment and biological reactor |
| CN101372664A (en) * | 2008-05-16 | 2009-02-25 | 北京航空航天大学 | Tissue engineering reactor having tissue cultures tension-compression and rotation functions |
| CN201704334U (en) * | 2010-05-27 | 2011-01-12 | 长江大学 | Looseness compensation device for tissue engineering bioreactor support |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014141136A1 (en) | 2013-03-13 | 2014-09-18 | Association For The Advancement Of Tissue Engineering And Cell Based Technologies And Therapies - A4Tec | Rotational dual chamber bioreactor: methods and uses thereof |
| US10125343B2 (en) | 2013-03-13 | 2018-11-13 | Association For The Advancement Of Tissue Engineering And Cell Based Technologies And Therapies—A4Tec | Rotational dual chamber bioreactor: methods and uses thereof |
| CN104046564A (en) * | 2014-06-06 | 2014-09-17 | 上海交通大学 | Physiological environment-imitating mechanical stimulation type biological reactor system |
| CN104046564B (en) * | 2014-06-06 | 2016-01-20 | 上海交通大学 | A kind of physiology environmental mechanics stimulates formula bioreactor system |
| CN110484426A (en) * | 2019-09-16 | 2019-11-22 | 常州市第一人民医院 | A kind of power stimulation loading device and its working method |
| CN111748472A (en) * | 2020-07-10 | 2020-10-09 | 深圳市第二人民医院(深圳市转化医学研究院) | Tendon tissue bionic culture ware |
| CN115369037A (en) * | 2022-10-24 | 2022-11-22 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Full circulation device, method and loading system for spinal culture solution |
| CN115369037B (en) * | 2022-10-24 | 2023-01-31 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Full circulation device, method and loading system for spinal culture solution |
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Application publication date: 20121128 |