CN102793670A - PS (Phosphatidylserine)-liposome and synthesis process thereof - Google Patents

Abstract

The invention relates to a liposome and a synthesis process thereof, in particular to a PS (Phosphatidylserine)-liposome and a synthesis process thereof, and belongs to the field of biomedicine. The PS-liposome is synthesized by a liposome, PS, phosphatidylcholine and chloroform. In the PS-liposome synthesis process, the PS-liposome comprises the following raw materials in percentage by volume: 40 percent to 55 percent of liposome, 30 percent to 48 percent of PS, 10 percent to 12 percent of phosphatidylcholine and 5 percent to 9 percent of chloroform. The PS-liposome has a small particle size, high entrapment efficiency and large surface tension, can reduce the drug toxicity, strengthen the pharmacologic action, reduce the removal rate of drug, prolong the action time of the drug and strengthen the in vivo and vitro stability of the drug, has low toxicity and has no immunosuppression effect on a human body. The process is simple to operate. No by-product is generated. The PS-liposome and the synthesis process thereof are safe and environment.

Description

A kind of PS-liposome and synthesis technique thereof

Technical field

The present invention relates to a kind of liposome and synthesis technique thereof, particularly a kind of PS-liposome and synthesis technique thereof belong to biomedicine field.

Background technology

21 century brain research will be the main flow of life science, along with the raising of China's living standards of the people and the aging of population, will become more urgent for the exploitation of central nervous system disease newtype drugs such as acute cerebrovascular disease, nervous system degeneration disease.

Liposome (liposome, or title lipoid bead, liquid crystal microcapsule) is the film material with lipoids such as phospholipid, cholesterol; Type of having membrane structure; Pretend carrier, can be engulfed, increase the anelasticity of medicine adenoid directivity and target tissue by mononuclear phagocyte system for medicine.In recent years biological study shows: bringing into play very important directional induction effect in the macrophage that Phosphatidylserine (being called for short PS) is organized as the label on film surface around, the process that vascular endothelial cell is engulfed apoptotic cell; Macrophage can produce transforming growth factor-beta anti-inflammatory media such as (Transforming growth factor-β TGF-β) after carrying out phagocytosis, the generation of restriction inflammatory reaction.Therefore, those skilled in the art are badly in need of seeking a kind of PS-liposome and synthesis technique thereof.

Summary of the invention

For solving the above-mentioned deficiency in the present technology, the purpose of this invention is to provide a kind of PS-liposome, this lipid physical ability reduces drug toxicity, strengthens pharmacological action, reduces the elimination speed of medicine, prolong drug action time, strengthens medicine inside and outside stability; Toxicity is little, and human body is not had immunosuppressive action.

Another object of the present invention is for a kind of synthesis technique of PS-liposome is provided, and this synthesis technique is simple and easy to control, technological requirement is low, and no coupling product in the production process.

For realizing above-mentioned purpose, the present invention realizes through following technical scheme:

A kind of PS-liposome, synthetic by following component: liposome, human body non essential amino acid and chloroform.

Described human body non essential amino acid is Phosphatidylserine and phosphatidylcholine.

A kind of PS-liposome synthesis technique may further comprise the steps:

(1), by volume percentage mix is even with liposome, Phosphatidylserine, phosphatidylcholine and chloroform;

(2), with the mixture dry distilling in (1);

(3), with the mixture vacuum drying of (2), add CMF-PBS then, make it to become to hang turbid appearance liquid;

(4), with the suspension in (3) with ultrasonic grinding to becoming transparent appearance liquid, centrifugal then, get supernatant;

(5), with the supernatant in (4) with Saltorius Filter (0.22 μ m) filtration sterilization, make the PS-liposome solutions.

Percent by volume in the described step (1) is:

Liposome 40%-55%

Phosphatidylserine 30%-48%

Phosphatidylcholine 10%-12%

Chloroform 5%-9%.

As preferably, a kind of synthesis technique of PS-liposome, the percent by volume in the described step (1) is:

Liposome 45%

Phosphatidylserine 38%

Phosphatidylcholine 10%

Chloroform 7%.

Centrifuging temperature in the described step (4) is 4 ℃.

PS-liposome of the present invention and synthesis technique thereof, its PS-liposome particle diameter is little, envelop rate is high, surface tension is big, can reduce drug toxicity, strengthens pharmacological action, reduces the elimination speed of medicine, prolong drug action time, strengthens medicine inside and outside stability; Toxicity is little, and human body is not had immunosuppressive action, and production technology is simple and easy to control, technological requirement is low, and no coupling product in the production process, safety and environmental protection.

The specific embodiment

Embodiment 1

45ml liposome, 38ml Phosphatidylserine, 10ml phosphatidylcholine are mixed with the 7ml chloroform; Dry distilling behind the mix homogeneously; Add CMF-PBS behind the product vacuum drying that dry distilling is come out and become outstanding turbid appearance liquid, cause into transparent appearance solution with ultrasonic grinding then, get supernatant after 4 ℃ of centrifugalize; With Saltorius Filter (0.22 μ m) filtration sterilization, make the PS-liposome solutions.

Embodiment 2

55ml liposome, 30ml Phosphatidylserine, 10ml phosphatidylcholine are mixed with the 5ml chloroform; Dry distilling behind the mix homogeneously; Add CMF-PBS behind the product vacuum drying that dry distilling is come out and become outstanding turbid appearance liquid, cause into transparent appearance solution with ultrasonic grinding then, get supernatant after 4 ℃ of centrifugalize; With Saltorius Filter (0.22 μ m) filtration sterilization, make the PS-liposome solutions.

Embodiment 3

40ml liposome, 39ml Phosphatidylserine, 12ml phosphatidylcholine are mixed with the 9ml chloroform; Dry distilling behind the mix homogeneously; Add CMF-PBS behind the product vacuum drying that dry distilling is come out and become outstanding turbid appearance liquid, cause into transparent appearance solution with ultrasonic grinding then, get supernatant after 4 ℃ of centrifugalize; With Saltorius Filter (0.22 μ m) filtration sterilization, make the PS-liposome solutions.

Embodiment 4

52ml liposome, 31ml Phosphatidylserine, 11ml phosphatidylcholine are mixed with the 6ml chloroform; Dry distilling behind the mix homogeneously; Add CMF-PBS behind the product vacuum drying that dry distilling is come out and become outstanding turbid appearance liquid, cause into transparent appearance solution with ultrasonic grinding then, get supernatant after 4 ℃ of centrifugalize; With Saltorius Filter (0.22 μ m) filtration sterilization, make the PS-liposome solutions.

Performance test

The PS-liposome that the foregoing description 1-4 is made carries out particle diameter, envelop rate and capillary detection.

The PS-liposome solutions is allocated to 0.3-0.6g/L with 10mmol/L ammonium acetate, 50mmol/L Tris solution (PH=7.5), dropped on the copper mesh of supporting film, filter paper blots; Drip ammonium molybdate again, filter paper blots, natural drying 30min; The structure and the size of electron microscopic observation PS-liposome; On electromicroscopic photograph, get 10 different visuals field at random, measure the diameter of PS-liposome respectively, this diameter multiply by corrective system 0.707 be PS-liposome particle diameter.

Measure PS-liposome solutions 0.5ml, be diluted to 5.0ml, under 4 ℃, with the centrifugal 30min of 37000r/min with PBS.Abandon supernatant to remove the PS of parcel, what be deposited on the bottom is liposome folliculus, and liposome folliculus is diluted to 1.0ml with PBS.Get 200uL liposome folliculus liquid and 100uL PS-liposome solutions respectively, add 100g/L Triton 100 dissolvings, measure wherein Protein content with Folin-phenol reagent method.In the liposome folliculus liquid in Protein content and the liposome solutions ratio of Protein content be the envelop rate of PS-liposome.

Get the PS-liposome and measure 5min with surface tension instrument.

Test result sees the following form.

Table

Embodiment	Particle diameter (um, n=30)	Envelop rate (%)	Surface tension (mN/m, n=4)
				Embodiment 1	0.463	60	13.25
Embodiment 2	0.451	62	13.55
				Embodiment 3	0.461	60	13.25
Embodiment 4	0.468	65	13.80

Know that by last table PS-liposome particle diameter of the present invention is little, envelop rate is high, surface tension is big, and production technology is simple and easy to control, technological requirement is low, and no coupling product in the production process, safety and environmental protection.

The foregoing description only is used to the inventive concept of the present invention of explaining, but not to the qualification of rights protection of the present invention, allly utilizes this design that the present invention is carried out the change of unsubstantiality, all should fall into protection scope of the present invention.

Claims (6)

1. PS-liposome is characterized in that: synthetic by following component:

Liposome, human body non essential amino acid and chloroform.

2. PS-liposome as claimed in claim 1 is characterized in that: described human body non essential amino acid is Phosphatidylserine and phosphatidylcholine.

3. PS-liposome synthesis technique is characterized in that: may further comprise the steps:

(1), by volume percentage mix is even with liposome, Phosphatidylserine, phosphatidylcholine and chloroform;

(2), with the mixture dry distilling in (1);

(3), with the mixture vacuum drying of (2), add CMF-PBS then, make it to become to hang turbid appearance liquid;

(4), with the suspension in (3) with ultrasonic grinding to becoming transparent appearance liquid, centrifugal then, get supernatant;

(5), with the supernatant in (4) with Saltorius Filter (0.22 μ m) filtration sterilization, make the PS-liposome solutions.

4. PS-liposome synthesis technique as claimed in claim 3 is characterized in that: the percent by volume in the described step (1) is:

Liposome 40%-55%

Phosphatidylserine 30%-48%

Phosphatidylcholine 10%-12%

Chloroform 5%-9%.

5. PS-liposome synthesis technique as claimed in claim 3 is characterized in that: the percent by volume in the described step (1) is:

Liposome 45%

Phosphatidylserine 38%

Phosphatidylcholine 10%

Chloroform 7%.

6. PS-liposome synthesis technique as claimed in claim 3 is characterized in that: the centrifuging temperature in the described step (4) is 4 ℃.