CN102784401A - Method for combining low temperature quick freezing and superhigh pressure virus inactivation of blood plasma - Google Patents
Method for combining low temperature quick freezing and superhigh pressure virus inactivation of blood plasma Download PDFInfo
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- CN102784401A CN102784401A CN2011101288474A CN201110128847A CN102784401A CN 102784401 A CN102784401 A CN 102784401A CN 2011101288474 A CN2011101288474 A CN 2011101288474A CN 201110128847 A CN201110128847 A CN 201110128847A CN 102784401 A CN102784401 A CN 102784401A
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- blood plasma
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Abstract
The invention belongs to a technology for treating a blood plasma through a physical method, and especially provides a method for combining low temperature quick freezing and superhigh pressure virus inactivation of the blood plasma to improve the superhigh pressure virus inactivation quality of the apheresis blood plasma. The method comprises the following processes: cooling the blood plasma before the superhigh pressure treatment of the blood plasma to -23DEG C to -25DEG C to make the blood plasma not be frozen, maintaining the temperature for 20-30min, putting the blood plasma in a critical freezing state in a superhigh pressure container, applying a superhigh pressure 200-400MPA and simultaneously cooling to -35DEG C to -40DEG C, maintaining the temperature for 30-40min, and releasing the pressure to complete the instant freezing and the virus inactivation of the blood plasma. Test results prove that the virus inactivation and freezing quality of the blood plasma treated through the method is very high, the used superhigh pressure is low, and an obvious economic effect is possessed.
Description
Technical field: the processing blood plasma technology that the invention belongs to physical method.It is specifically related to the method that a kind of low-temperature quick-freezing of blood plasma combines with the supertension inactivation of virus.
Background technology: in recent years, delivered patent and article both at home and abroad, the ultrahigh-pressure cold freeze techniques is also arranged simultaneously about supertension deactivation plasma viral.Find that in experiment water applies 200MPa in the time of-20 ℃ pressure can not freeze, but can produce icing phenomenon of moment after the pressure release suddenly.The quick-freezing of present blood plasma blood bag needs 40 minutes blood bag centers just can reach-30 ℃, and ice crystal is grown up in freezing process, often plasma fraction is damaged to some extent, and cooling time is long more, and the degree of damage is big more.And under the pressure of supertension 200-400MPa; Even blood plasma reaches-30 ℃ and also can not freeze, but the moment of pressure release blood plasma can accomplish freezing process and become solid-stately, and the crystal grain that forms is very fine; Can protect the composition of blood plasma not to be destroyed effectively, the effect that improves the blood plasma quality is arranged.Under-30 to-40 ℃ of temperature conditions, apply the pressure of 200-400MPa, the virus in can deactivation blood plasma has guaranteed the safety of blood transfusion; After pressure discharged, blood plasma moment freezed, and has improved the quality of freezing of blood plasma.
The method that the low-temperature quick-freezing of summary of the invention: propose new method for the quality that improves the plasma treatment inactivation of virus is special---blood plasma combines with the supertension inactivation of virus; Cooling simultaneously in the process of exerting pressure; Can reach the quality of better inactivation of viruses, tangible economic effect is arranged.
By as above design, the low-temperature quick-freezing of the blood plasma that this programme provided with the characteristic of the method that the supertension inactivation of virus combines is: its technical process is following:
1. Plasma Pheresis/Apheresis Plasma is packed in the blood bag, air is sealed in the dehematize bag side by side;
2. the blood bag is placed on pre-cooling under-22～-25 ℃ the condition, cooled off 20～30 minutes;
3. the plasma bags after the pre-cooling is put into the ultrahigh pressure vessel of the ultra high pressure treatment equipment that has quick refrigerator;
4. apply supertension 200～400MPA, be cooled to-35～-40 ℃ simultaneously and kept 30～40 minutes;
5. after accomplishing the processing of quick-freezing and inactivation of viruses of blood plasma, take out the plasma bags warehouse-in and preserve.
Adopt this programme can embody following superiority: blood plasma does not freeze when 1. being pre-chilled to-25 ℃, but near solidification point, therefore can improve the speed of quick-freezing, and ultra high pressure treatment can obtain better effect; 2. because temperature is lower, compare, reach the treatment effect of same inactivation of viruses, can reduce the pressure value of supertension, help protecting the effective ingredient in the blood plasma, particularly thrombin with room temperature; 3. owing to be to adopt the physical method inactivation of viruses, avoided the drug residue negative effect of chemical method inactivation of viruses, the safety that has improved blood plasma; 4. adopt this new method not only to simplify the technology of inactivation of viruses, reduced the processing cost of blood plasma, and quick-freezing and the virus of going out accomplishes simultaneously, tangible economic effect is arranged.
The specific embodiment: the characteristics of this technical process are that blood plasma is cooled to-23～-25 ℃ earlier, keep 20～30 minutes, and this moment, blood plasma did not freeze as yet; Add supertension 200～400MPA; Simultaneously, temperature drops to-35～-40 ℃ and kept 30～40 minutes, accomplishes the entire process process.The plasma bags of taking-up in ultrahigh pressure vessel, warehouse-in is preserved.The blood plasma quality of handling through this kind of certificate of analysis method is high, and used supertension numerical value is low, and tangible economic effect is arranged.
Claims (1)
1. the low-temperature quick-freezing of a blood plasma and the method that the supertension inactivation of virus combines is characterized in that its technical process is following:
1. Plasma Pheresis/Apheresis Plasma is packed in the blood bag, air is sealed in the dehematize bag side by side;
2. the blood bag is placed on pre-cooling under-22～-25 ℃ the condition, cooled off 20～30 minutes;
3. the plasma bags after the pre-cooling is put into the ultrahigh pressure vessel of the ultra high pressure treatment equipment that has quick refrigerator;
4. apply supertension 200～400MPA, be cooled to-35～-40 ℃ simultaneously and kept 30～40 minutes;
5. after accomplishing the processing of quick-freezing and inactivation of viruses of blood plasma, take out the plasma bags warehouse-in and preserve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101288474A CN102784401A (en) | 2011-05-18 | 2011-05-18 | Method for combining low temperature quick freezing and superhigh pressure virus inactivation of blood plasma |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101288474A CN102784401A (en) | 2011-05-18 | 2011-05-18 | Method for combining low temperature quick freezing and superhigh pressure virus inactivation of blood plasma |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102784401A true CN102784401A (en) | 2012-11-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101288474A Pending CN102784401A (en) | 2011-05-18 | 2011-05-18 | Method for combining low temperature quick freezing and superhigh pressure virus inactivation of blood plasma |
Country Status (1)
| Country | Link |
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| CN (1) | CN102784401A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105854039A (en) * | 2016-05-21 | 2016-08-17 | 郑州飞龙医疗设备有限公司 | Ultrahigh pressure inactivation method and equipment for pathogens in liquid biological products |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6270723B1 (en) * | 1998-06-15 | 2001-08-07 | Bbi Bioseq, Inc. | Rapid cryobaric sterilization and vaccine preparation |
-
2011
- 2011-05-18 CN CN2011101288474A patent/CN102784401A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6270723B1 (en) * | 1998-06-15 | 2001-08-07 | Bbi Bioseq, Inc. | Rapid cryobaric sterilization and vaccine preparation |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105854039A (en) * | 2016-05-21 | 2016-08-17 | 郑州飞龙医疗设备有限公司 | Ultrahigh pressure inactivation method and equipment for pathogens in liquid biological products |
| CN105854039B (en) * | 2016-05-21 | 2019-01-25 | 郑州飞龙医疗设备有限公司 | To the super-pressure ablation method and equipment of pathogen in liquid bio product |
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Application publication date: 20121121 |