CN102776254A - Preparation method of high-concentration acrylamide aqueous solution by microbiology - Google Patents
Preparation method of high-concentration acrylamide aqueous solution by microbiology Download PDFInfo
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- CN102776254A CN102776254A CN2012102516294A CN201210251629A CN102776254A CN 102776254 A CN102776254 A CN 102776254A CN 2012102516294 A CN2012102516294 A CN 2012102516294A CN 201210251629 A CN201210251629 A CN 201210251629A CN 102776254 A CN102776254 A CN 102776254A
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Abstract
The invention discloses a preparation method of high-concentration acrylamide aqueous solution by microbiology. The preparation method includes strain domestication and screening, free cell preparation, and multistage continuous serial reaction. The preparation method has the advantages that the common one-step intermittent reaction is replaced by the multistage continuous serial reaction, acrylamide is produced from the free cells by microbiological hydration, processes of reactors are controlled, bacteria are separated, utilization of the strain is increased, 60% high-concentration acrylamide can be obtained, the requirements of market for acrylamide are met, and market competitiveness of products is improved.
Description
Technical field
The present invention relates to a kind of preparation method of high concentration microorganism method acrylamide solution.
Background technology
Acrylic amide production has chemical method and biological process, and chemical method is generally produced content 16%～20%, and the microbial method hydration goes out still concentration generally 25～30%; Development along with the acrylic amide industry; Market is increasing to the demand of high density acrylic amide, and the acrylic amide demand of general concentration 40～50% is bigger, and some particular client maximum concentration demands reach 55～60%; Common customer is to dissolve configuration again with acrylic amide dry powder water when demand high density acrylic amide basically; Or concentrate with the heating of 30% acrylic amide and to obtain, the cost overall situation is polluted greatly like this, and the easy autohemagglutination of concentration process.
Summary of the invention
The objective of the invention is to overcome the deficiency of above-mentioned prior art; Produce acrylic amide through mikrobe free cell hydration method; Changing a step intermittent reaction commonly used is the plural serial stage successive reaction; Improve the bacterial classification utilization ratio, a kind of preparation method of high concentration microorganism method acrylamide solution is provided.
In order to solve the problems of the technologies described above, technical scheme of the present invention is: a kind of preparation method of high concentration microorganism method acrylamide solution, comprise strain domestication screening, the preparation of free cell, multistage continuous cascade reaction, and it is characterized in that:
1) strain domestication screening: at first the Nocardia bacteria bacterial classification is carried out the inducing and acclimating screening and obtain a kind of good bacterial classification; Through the TE that batch feeding is cultivated and anti-substrate product suppresses; Improve the tolerance of bacterial classification; Obtain a kind of tolerance and improve 30%, enzymic activity is up to the bacterial strain of 2,500 ten thousand U/ml;
2) preparation of free cell: the strain fermentation cultivation to screening obtains fermented liquid; With the centrifugal removal impurity of fermented liquid; The solid thalline adds dehydration salt wash cycles through microfiltration membrane and filters, and finally obtains cleaning qualified mycelium, specific conductivity≤50Us/cm, colourity≤10 Hazen;
3) multistage continuous cascade reaction: in the plural serial stage reactor drum; Adopt the reaction of free cell direct hydration; Keep certain concentration in each stage reactor, calculate vinyl cyanide and the water yield and the every stage reactor load hourly that every order reaction per hour need drop into, control reaction temperature 18-20 ℃ according to the concentration gradient between load and every grade; Adopt ultra-filtration membrane to carry out separating of material and thalline between each stage reactor; And send into the next stage reactor drum to material continuously, whole process adopts DCS to control automatically, guarantees the continous-stable operation between each stage reactor.
Preferably, described plural serial stage reactor drum is 8 5m
3Stainless steel cauldron.
Preferably; Keep certain concentration in described each stage reactor, its specific requirement is: first step reactor drum concentration stabilize 30%, second stage concentration 40%, third stage concentration 45%, the fourth stage 50%, level V 53%, the 6th grade of 56%, the 7th grade of concentration 58%, the 8th grade of concentration 60%.
Preferably, the load of described the 8th grade of still per hour is 4 ton 60% a acrylic amide.
Preferably, every reaction kettle is equipped with 40 ultra-filtration membranes.
Beneficial effect of the present invention: adopt the plural serial stage successive reaction, improve the utilization ratio of bacterial classification, obtain 60% high density acrylic amide, satisfy the concentration demand of market, improve this competitiveness of product in market to acrylic amide.
Embodiment
Practical implementation step of the present invention is following:
1, bacterial classification Cycle Screening: at first the Nocardia bacteria bacterial classification is carried out the inducing and acclimating screening and obtain a kind of good bacterial classification; Through the TE that batch feeding is cultivated and anti-substrate product suppresses; Improve the tolerance of bacterial classification; Obtain a kind of tolerance and improve 30%, enzymic activity is up to the bacterial strain of 2,500 ten thousand U/ml.
2, the preparation of free cell:, under aseptic condition, bacterial classification inoculation to fermention medium is cultivated for the bacterial classification of step 1 screening; Temperature is 28 ± 2 ℃; Mixing speed is set at 150 ~ 200r/min, and air flow is 40L/min,, fermentation obtains fermented liquid behind the 40-50h; Fermented liquid is adopted water-soluble substances and impurity in the centrifugal removal fermented liquid of high rotating speed disk plate centrifuge; Solid thalline after the separation adds dehydration salt wash cycles through microfiltration membrane and filters, and finally obtains cleaning qualified mycelium, specific conductivity≤50Us/cm, colourity≤10 Hazen.
Wherein, fermentation culture based component: glucose 20-25g/l, K
2HPO
40.6-0.8g/l, yeast extract 7.0-9.0g/l, MgSO
47H
20 1.2-1.3 g/l, potassium hydrogenphosphate 0.6-0.8g/l, monosodium glutamate 1.4-1.5 g/l, urea 7.0-8.0 g/l, skimmer 100ppm.
3, multistage continuous cascade reaction: at 8 5m
3Stainless steel cauldron carry out the free cell hydration reaction, control reaction temperature 18-20 ℃, in first step reaction kettle; With 4t water, the mycelium of 300-400kg drops in the reaction kettle, opens and stirs; Stream adds vinyl cyanide and carries out the enzyme catalysis hydration reaction; When acrylamide concentration is accumulated to 30%, adopts ultra-filtration membrane to carry out separating of material and thalline, and send into second reactor to material continuously; In second reactor, drop into the 300-400kg mycelium, add vinyl cyanide and water generation hydration reaction again; When the concentration of acrylic amide can be accumulated to 40%, material is sent into the next stage reactor drum, following six stage reactors through ultrafiltration membrance filter; Repeat above reinforced process; Keep simultaneously certain concentration gradient in each stage reactor, control third stage reactor drum concentration stabilize 45%, the fourth stage is 50%, level V 53%, the 6th grade of 56%, the 7th grade of concentration 58%, the 8th grade of concentration 60% discharging, realizes continuous cascade reaction like this; Finally obtaining load per hour is 4 tons high density 60% acrylic amide; This technology ability continuous and stable production is more than 48 hours, and whole process adopts DCS to control automatically, guarantees the continous-stable operation between each stage reactor.
Claims (5)
1. the preparation method of a high concentration microorganism method acrylamide solution comprises strain domestication screening, the preparation of free cell, multistage continuous cascade reaction, it is characterized in that:
1) strain domestication screening: at first the Nocardia bacteria bacterial classification is carried out the inducing and acclimating screening and obtain a kind of good bacterial classification; Through the TE that batch feeding is cultivated and anti-substrate product suppresses; Improve the tolerance of bacterial classification; Obtain a kind of tolerance and improve 30%, enzymic activity is up to the bacterial strain of 2,500 ten thousand Ug/ml;
2) preparation of free cell: the strain fermentation cultivation to screening obtains fermented liquid; With the centrifugal removal impurity of fermented liquid; The solid thalline adds dehydration salt wash cycles through microfiltration membrane and filters, and finally obtains cleaning qualified mycelium, specific conductivity≤50Us/cm, colourity≤10Hazen;
3) multistage continuous cascade reaction: in the plural serial stage reactor drum; Adopt the reaction of free cell direct hydration, keep certain concentration in each stage reactor, calculate vinyl cyanide and the water yield and the every stage reactor load hourly that every order reaction per hour need drop into according to the concentration gradient between load and every grade; Control reaction temperature 18-20 ℃; Each stage reactor keeps certain concentration gradient, adopts ultra-filtration membrane to carry out separating of material and thalline between each stage reactor, and sends into the next stage reactor drum to material continuously; Whole process adopts DCS to control automatically, guarantees the continous-stable operation between each stage reactor.
2. the preparation method of a kind of high concentration microorganism method acrylamide solution according to claim 1 is characterized in that: described plural serial stage reactor drum is 8 5m
3Stainless steel cauldron.
3. the preparation method of a kind of high concentration microorganism method acrylamide solution according to claim 1; It is characterized in that: the concentration gradient of described each stage reactor, its specific requirement is: first step reactor drum concentration stabilize 30%, second stage concentration 40%, third stage concentration 45%, the fourth stage 50%, level V 53%, the 6th grade of 56%, the 7th grade of concentration 58%, the 8th grade of concentration 60%.
4. the preparation method of a kind of high concentration microorganism method acrylamide solution according to claim 1 is characterized in that: the load of described the 8th grade of still per hour is 4 ton 60% a acrylic amide.
5. the preparation method of a kind of high concentration microorganism method acrylamide solution according to claim 1 is characterized in that: every reaction kettle is equipped with 40 ultra-filtration membranes.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107779482A (en) * | 2017-12-05 | 2018-03-09 | 山东宝莫生物化工股份有限公司 | A kind of production technology of high concentration acrylamide |
| CN109652474A (en) * | 2018-12-26 | 2019-04-19 | 安徽巨成精细化工有限公司 | The method of biocatalysis acrylonitrile hydration reaction, the ablation method for producing nitrile bacterial strain |
| CN115678930A (en) * | 2023-01-03 | 2023-02-03 | 济宁南天农科化工有限公司 | Preparation method of acrylamide |
-
2012
- 2012-07-20 CN CN2012102516294A patent/CN102776254A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107779482A (en) * | 2017-12-05 | 2018-03-09 | 山东宝莫生物化工股份有限公司 | A kind of production technology of high concentration acrylamide |
| CN109652474A (en) * | 2018-12-26 | 2019-04-19 | 安徽巨成精细化工有限公司 | The method of biocatalysis acrylonitrile hydration reaction, the ablation method for producing nitrile bacterial strain |
| CN115678930A (en) * | 2023-01-03 | 2023-02-03 | 济宁南天农科化工有限公司 | Preparation method of acrylamide |
| CN115678930B (en) * | 2023-01-03 | 2023-04-07 | 济宁南天农科化工有限公司 | Preparation method of acrylamide |
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Application publication date: 20121114 |