CN102768242A - Method for determining content of oral liquid of Chinese wolfberry and rehmanniae - Google Patents
Method for determining content of oral liquid of Chinese wolfberry and rehmanniae Download PDFInfo
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- CN102768242A CN102768242A CN2011101115507A CN201110111550A CN102768242A CN 102768242 A CN102768242 A CN 102768242A CN 2011101115507 A CN2011101115507 A CN 2011101115507A CN 201110111550 A CN201110111550 A CN 201110111550A CN 102768242 A CN102768242 A CN 102768242A
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- paeoniflorin
- reference substance
- oral liquid
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Abstract
An oral liquid of Chinese wolfberry and rehmanniae has an effect of nourishing the kidney and liver and is used to treat deficiency of liver-yin and kidney-yin, vertigo, tinnitus, photophobia and dim sight. According to the invention, the content of the oral liquid is determined by high performance liquid chromatography (HPLC); octadecylsilane chemically bonded silica is used as a filler, acetonitrile-water (30:80) is used as a mobile phase; measurement wavelength is 245 nm, and the number of theoretical plates is calculated according to paeoniflorin peak and should not be lower than 1500. The method for determining the content of the oral liquid of Chinese wolfberry and rehmanniae is simple to operate, has good stability, and is in favor of controlling the quality of the product.
Description
Technical field
The present invention relates to the field of Chinese medicines, especially a kind of content assaying method of QIJU DIHUANG KOUFUYE.
Background technology
QIJU DIHUANG KOUFUYE has the effect of nourishing the kidney and the liver, is used for the deficiency of liver-yin and kidney-yin, dizzy tinnitus, photophobia photophobia, blurring of vision.The content assaying method step of traditional QIJU DIHUANG KOUFUYE is comparatively loaded down with trivial details, and the result of test is accurate inadequately, is unfavorable for the quality of product is controlled.
Summary of the invention
The technical matters that the present invention will solve is: provide a kind of science the easy content assaying method for QIJU DIHUANG KOUFUYE.
The technical solution adopted for the present invention to solve the technical problems is: the assay of described QIJU DIHUANG KOUFUYE adopts according to high performance liquid chromatography (VI D of Chinese Pharmacopoeia version in 2005) and measures; Step is following: the system suitability test uses octadecylsilane chemically bonded silica to be filling agent, and acetonitrile-water (30: 80) is a moving phase; The detection wavelength is 245nm, and number of theoretical plate is pressed the Paeoniflorin peak and calculated, and should be not less than 1500;
It is an amount of that the preparation precision of reference substance solution takes by weighing the Paeoniflorin reference substance, adds moving phase solution and process the solution that every 1ml contains 30 μ g;
The preparation precision of need testing solution is measured these article 2ml, puts in the separating funnel, extracts four times with water saturated normal butyl alcohol, each 2ml; Obtain upper solution, water bath method, residue add moving phase solution makes dissolving in right amount, in the dislocation 10ml measuring bottle; And be diluted to scale, and measure, promptly get;
Determination method is accurate draws reference substance solution 5 μ l and need testing solution an amount of (looking content just decides), injects liquid chromatograph, measures, and promptly gets.
The every 1ml of these article contains moutan bark with Paeoniflorin (C
23H
28O
11) meter, must not be less than 0.05mg.
The invention has the beneficial effects as follows that relatively science is easy again for the content assaying method of QIJU DIHUANG KOUFUYE of the present invention, good stability helps product quality is controlled.
Embodiment
Now the present invention is done further detailed explanation.
The assay of QIJU DIHUANG KOUFUYE adopts according to high performance liquid chromatography (VI D of Chinese Pharmacopoeia version in 2005) and measures, and step is following:
The system suitability test uses octadecylsilane chemically bonded silica to be filling agent, and acetonitrile-water (30: 80) is a moving phase; The detection wavelength is 245nm, and number of theoretical plate is pressed the Paeoniflorin peak and calculated, and should be not less than 1500;
It is an amount of that the preparation precision of reference substance solution takes by weighing the Paeoniflorin reference substance, adds moving phase solution and process the solution that every 1ml contains 30 μ g;
The preparation precision of need testing solution is measured these article 2ml, puts in the separating funnel, extracts four times with water saturated normal butyl alcohol, each 2ml; Obtain upper solution, water bath method, residue add moving phase solution makes dissolving in right amount, in the dislocation 10ml measuring bottle; And be diluted to scale, and measure, promptly get;
Determination method is accurate draws reference substance solution 5 μ l and need testing solution an amount of (looking content just decides), injects liquid chromatograph, measures, and promptly gets.
The every 1ml of these article contains moutan bark with Paeoniflorin (C
23H
28O
11) meter, must not be less than 0.05mg.
Claims (2)
1. the content assaying method of a QIJU DIHUANG KOUFUYE is characterized in that: assay employing photograph high performance liquid chromatography (VI D of Chinese Pharmacopoeia version in the 2005) mensuration of described QIJU DIHUANG KOUFUYE.
2. the content assaying method of QIJU DIHUANG KOUFUYE according to claim 1, it is characterized in that: step is following:
The system suitability test uses octadecylsilane chemically bonded silica to be filling agent, and acetonitrile-water (30: 80) is a moving phase; The detection wavelength is 245nm, and number of theoretical plate is pressed the Paeoniflorin peak and calculated, and should be not less than 1500;
It is an amount of that the preparation precision of reference substance solution takes by weighing the Paeoniflorin reference substance, adds moving phase solution and process the solution that every 1ml contains 30 μ g;
The preparation precision of need testing solution is measured these article 2ml, puts in the separating funnel, extracts four times with water saturated normal butyl alcohol, each 2ml; Obtain upper solution, water bath method, residue add moving phase solution makes dissolving in right amount, in the dislocation 10ml measuring bottle; And be diluted to scale, and measure, promptly get;
Determination method is accurate draws reference substance solution 5 μ l and need testing solution an amount of (looking content just decides), injects liquid chromatograph, measures, and promptly gets.
The every 1ml of these article contains moutan bark with Paeoniflorin (C
23H
28O
11) meter, must not be less than 0.05mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101115507A CN102768242A (en) | 2011-04-30 | 2011-04-30 | Method for determining content of oral liquid of Chinese wolfberry and rehmanniae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101115507A CN102768242A (en) | 2011-04-30 | 2011-04-30 | Method for determining content of oral liquid of Chinese wolfberry and rehmanniae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102768242A true CN102768242A (en) | 2012-11-07 |
Family
ID=47095715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101115507A Pending CN102768242A (en) | 2011-04-30 | 2011-04-30 | Method for determining content of oral liquid of Chinese wolfberry and rehmanniae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102768242A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108562681A (en) * | 2018-06-28 | 2018-09-21 | 江苏海昇药业有限公司 | The quantitative detection method of QIJU DIHUANG KOUFUYE multicomponent |
-
2011
- 2011-04-30 CN CN2011101115507A patent/CN102768242A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108562681A (en) * | 2018-06-28 | 2018-09-21 | 江苏海昇药业有限公司 | The quantitative detection method of QIJU DIHUANG KOUFUYE multicomponent |
| CN108562681B (en) * | 2018-06-28 | 2019-04-05 | 江苏海昇药业有限公司 | The quantitative detection method of QIJU DIHUANG KOUFUYE multicomponent |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20121107 |