CN102763660B - Water aqua for growth of bean sprout vegetable and preparation method thereof - Google Patents
Water aqua for growth of bean sprout vegetable and preparation method thereof Download PDFInfo
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Abstract
The invention provides a water aqua for growth of bean sprout vegetable and a preparation method thereof. The water aqua is composed of active ingredient gibberellin, 6-benayl aminopurine, surfactant polysorbate, organic solvent and water. The surfactant polysorbate is added so as to slow down resolving of the active ingredient gibberellin and the 6-benayl aminopurine in water, and water aqua stability is guaranteed. The invention further provides the preparation method of the water aqua and a detecting method of active ingredients. Experimental results show that by using the water aqua, growth of bean sprout fibrous roots can be effectively restrained, the number of the bean sprout fibrous roots is reduced, root length is reduced, bean sprout is thick, quality of the bean sprout is improved, and useful effects are obtained. By means of product residue research and risk estimation, the water aqua is safe to be used for the growth of the bean sprout vegetable.
Description
Technical field
The present invention relates to a kind of inhibition plant growth regulator, be specifically related to a kind of aqua its preparation method for the growth of beans tooth dish.
Background technology
In the production process of bean sprouts, have golden yellow cotyledon, shorter root system does not have the bean sprouts of fibrous root to receive that the consumer favors.And in actual production process, the bean sprouts of producing often root system is longer, and fibrous root is more; Therefore, the bean sprout growth conditioning agent of making good use of is regulated and control, the bean sprouts of high-quality is produced very important meaning.
At present, domestic have multiple bean sprout growth conditioning agent, but recommendable be growth regulator by the preparation of gibberellin, 6-benzyl aminopurine bi-component.
Gibberellin belongs to the antibiotics agricultural chemicals, is a kind of broad-spectrum plant growth regulator, is one of five large plant hormones.It can promote the elongation of cell, stem, increases plant height, can promote the growth of hereditary dwarfed plant; Break the organ dormancy such as seed, stem tuber and bulb of some vegetables, improve germination rate, play low temperature vernalization and long-day to act on, promote and induce long day vegetable to bloom then; Promote that vegetables bear fruit, the growing of fruit retention and fruit.Gibberellin effect under the adverse environmental factors such as low temperature, arid, the low light level or short-day is more remarkable.
6-benzyladenine (6-benzylaminopurine) claims again N-benzyl adenosine, is called for short 6-BA, is a kind of artificial synthetic auxin, to the growth of plant, grow to have and promote and regulating action.
The growth regulator of gibberellin, the preparation of 6-benzyl aminopurine bi-component is used for bean sprout growth, can grow by establishment bean sprouts fibrous root, reduces bean sprouts fibrous root quantity, shortens root long, under the suitable concentration, significantly improves bean sprouts output, improves quality.
Chinese patent 01128492.7 discloses the growth regulator that gibberellin, 6-benzyl aminopurine bi-component are prepared, and the mass ratio of gibberellin, 6-benzyl aminopurine is 1:2-5, and this bi-component is the pulvis that packs respectively, is formulated as aqua during use.Compound method is: the compound concentration of 6-benzyl aminopurine is 20ppm in solution, gibberellin 40-100ppm; Method is that the former mixes fixed molten with hydrochloric acid or edible white vinegar hydrotropy, the latter with ethanol or liquor hydrotropy again after both dissolve respectively.
Gibberellin, 6-benzyl aminopurine are the raw materials of less stable in water, therefore, Chinese patent 01128492.7 is made pulvis with gibberellin, 6-benzyl aminopurine, but bring very large trouble to the user, particularly, loaded down with trivial details preparation very easily causes mistake because of makers-up's difference, and preparing excessive conditioning agent can work the mischief to human body.
Simultaneously, the mass ratio of gibberellin, 6-benzyl aminopurine is that 1:2-5 does not take in risk assessment through benayl aminopurine in the bean sprouts and gibberellic acid meals.
Therefore, develop a kind of stablely, the gibberellin of edible safety, 6-benzyl aminopurine bi-component aqua are our problems anxious to be resolved.
Summary of the invention
The invention provides a kind of stable, the gibberellin of edible safety, 6-benzyl aminopurine bi-component aqua and new prescription, new prescription is by active component gibberellin, 6-benzyl aminopurine, and surfactant, organic solvent, water form.
The characteristics of new prescription are:
1. gibberellin: 6-benzyl aminopurine=4:2.9-3.1 w/w.
2. added the surfactant tween in the aqua.The adding of surfactant tween, so that aqua is stable, upper without oil slick, lower to precipitation, limpid, transparent.Its beneficial effect is seen embodiment 4.
3. mainly be: the adding of surfactant tween, so that active component gibberellin, 6-benzyl aminopurine decompose in water and slow down, through optimization formula being carried out the mensuration of the former medicine resolution ratio in heat storage front and back, the resolution ratio of two kinds of active ingredients all＜5% before and after the storage of aqua heat, meet the requirement of production, its beneficial effect is seen embodiment 5.
The invention provides a kind of stablely, the prescription of the gibberellin of edible safety, 6-benzyl aminopurine bi-component aqua: each amounts of components (mass fraction %) is in the aqua prescription:
Raw material form consumption (mass fraction %)
Gibberellin 1.6
6-benzyladenine 1.16-1.24
Acetic acid 25.0
Ethanol 15.0
Tween 4.8-5.2
Running water polishing to 100.
The invention provides a kind of stable, the gibberellin of edible safety, 6-benzyl aminopurine bi-component aqua, its preparation method is as follows:
Prescription: each amounts of components (mass fraction %) is in the aqua prescription:
Raw material form consumption (mass fraction %)
Gibberellin 1.6
6-benzyladenine 1.16-1.24
Acetic acid 25.0
Ethanol 15.0
Tween 4.8-5.2
Running water polishing to 100.
Preparation technology:
(1) in stainless steel cask, add 1.6 parts of gibberellin (quality), 15.0 parts of ethanol (quality), stirring and dissolving, solution enters material-compound tank;
(2) in stainless steel cask, add 6-benzyladenine 1.16-1.24 part (quality), 25.0 parts of acetic acid (quality), stirring and dissolving, solution enters material-compound tank;
(3) in material-compound tank, add tween 4.8-5.2 part (quality), the running water polishing stirred 60 minutes to 100 parts of solution total amounts (quality);
(4) filtering solution;
(5) test solution, filtrate are filled in infusion bottle or the bag;
(6) filled with solution is entered in the container;
(7) packing.
The present invention also provides the detection of aqua active component, and its active component detects with high performance liquid chromatography, and operating condition is as follows:
Mobile phase: acetonitrile: 0.1% glacial acetic acid aqueous solution (V:V)=40:60;
Flow: 1.0ml/min;
Column temperature: room temperature;
Detect wavelength: gibberellin 207nm, 6-benzyl aminopurine 267nm;
Sampling volume: 5 μ L;
Retention time (min): gibberellin 2.9min, 6-benzyl aminopurine 9.7min.
The invention provides a kind of stablely, the gibberellin of edible safety, 6-benzyl aminopurine bi-component aqua, aqua of the present invention are used for bean sprout growth, can grow by establishment bean sprouts fibrous root, reduce bean sprouts fibrous root quantity, shorten root long, under the suitable concentration, significantly improve bean sprouts output, improve quality.Its beneficial effect is seen embodiment 6.
The invention provides a kind of stablely, the gibberellin of edible safety, 6-benzyl aminopurine bi-component aqua are used for bean sprout growth to aqua of the present invention and have carried out safety evaluation, proves that aqua of the present invention is safe, test and see embodiment 7.
Indication beans tooth dish of the present invention comprises the tooth dish that the beans such as mung bean, soya bean, red bean, black soya bean generate.
Embodiment
Following embodiment is used for further narration the present invention, but does not impose any restrictions.
One of embodiment 1 aqua preparation technology
Prescription: each amounts of components is in the aqua prescription:
Raw material form consumption
1.6 kilograms of gibberellin
1.2 kilograms of 6-benzyladenines
25 kilograms of acetic acid
15 kilograms of ethanol
5. kilograms of polysorbate40s
Running water polishing to 100 kilogram.
Preparation technology:
(1) in stainless steel cask, add 1.6 kilograms of gibberellin, 15 kilograms of ethanol, stirring and dissolving, solution enters material-compound tank;
(2) in stainless steel cask, add 1.2 kilograms of 6-benzyladenines, 25 kilograms of acetic acid, stirring and dissolving, solution enters material-compound tank;
(3) in material-compound tank, add 5 kilograms of polysorbate40s, the running water polishing stirred 60 minutes to 100 kilograms of solution total amounts;
(4) filtering solution;
(5) test solution, filtrate are filled in infusion bottle or the bag;
(6) filled with solution is entered in the container;
(7) packing.
Embodiment 2 aqua preparation technologies' two
Prescription: each amounts of components is in the aqua prescription:
Raw material form consumption
1.6 kilograms of gibberellin
1.24 kilograms of 6-benzyladenines
25 kilograms of acetic acid
15 kilograms of ethanol
5.2 kilograms of polysorbate60s
Running water polishing to 100 kilogram.
Preparation technology:
(1) in stainless steel cask, add 1.6 kilograms of gibberellin, 15 kilograms of ethanol, stirring and dissolving, solution enters material-compound tank;
(2) in stainless steel cask, add 1.24 kilograms of 6-benzyladenines, 25 kilograms of acetic acid, stirring and dissolving, solution enters material-compound tank;
(3) in material-compound tank, add 5.2 kilograms of polysorbate60s, the running water polishing stirred 60 minutes to 100 kilograms of solution total amounts;
(4) filtering solution;
(5) test solution, filtrate are filled in infusion bottle or the bag;
(6) filled with solution is entered in the container;
(7) packing.
Embodiment 3 aqua preparation technologies' three
Prescription: each amounts of components is in the aqua prescription:
Raw material form consumption
1.6 kilograms of gibberellin
1.16 kilograms of 6-benzyladenines
25 kilograms of acetic acid
15 kilograms of ethanol
4.8 kilograms of Tween 80s
Running water polishing to 100 kilogram.
Preparation technology:
(1) in stainless steel cask, add 1.6 kilograms of gibberellin, 15 kilograms of ethanol, stirring and dissolving, solution enters material-compound tank;
(2) in stainless steel cask, add 1.16 kilograms of 6-benzyladenines, 25 kilograms of acetic acid, stirring and dissolving, solution enters material-compound tank;
(3) in material-compound tank, add 4.8 kilograms of Tween 80s, the running water polishing stirred 60 minutes to 100 kilograms of solution total amounts;
(4) filtering solution;
(5) test solution, filtrate are filled in infusion bottle or the bag;
(6) filled with solution is entered in the container;
(7) packing.
Embodiment 4 surfactants affect aqua
According to the preparation technology of aqua of the present invention, the amount of surfactant adopts amount in the table 1 in the technique, and Surfactant screens.
The domestic food emulsifier mainly comprises fatty acid glyceride, sucrose fatty ester, soybean lecithin, stearoyl lactate (or calcium) and this dish (Span) and tween (Tween) series at present, because the production cost of front four kinds of emulsifier is higher, this experiment selects span and tween series emulsifier to screen, and the surfactant screening sees Table 1:
| Surface-active | Consumption (%) | Outward appearance |
| Blank | Nothing | Muddy |
| This dish 40 | 4～8 | Muddy |
| This dish 60 | 4～8 | Muddy |
| Span 80 | 4～8 | Muddy |
| Polysorbate40 | 4～8 | Limpid, transparent |
| Polysorbate60 | 4～8 | Limpid, transparent |
| Tween 80 | 4～8 | Limpid, transparent |
Conclusion: the adding of surfactant tween, so that aqua is stable, upper without oil slick, lower to precipitation, limpid, transparent.
Embodiment 5The surfactant tween affects aqua active component resolution ratio
To carry out stability test by the aqua of embodiment 1 preparation, and be made as the A group, control group is not for adding the aqua of surfactant, and other compositions and embodiment 1 are same, establish the B group.
2 groups of aquas are carried out heat storage stability [(54 ± 2) ℃, 14 d] test simultaneously, be about to A group, B group under the condition of (54 ± 2) ℃, heat 14 days, the resolution ratio of heat storage front and back active component gibberellin, 6-benzyl aminopurine sees Table 2:
| The A group | Content (%) before the heat storage | Content (%) after the heat storage | Resolution ratio % |
| Gibberellin | 1.61 | 1.57 | 2.48 |
| 6-benzyl aminopurine | 1.21 | 1.17 | 3.31 |
| The B group | Content (%) before the heat storage | Content (%) after the heat storage | Resolution ratio % |
| Gibberellin | 1.60 | 1.43 | 10.63 |
| 6-benzyl aminopurine | 1.19 | 10.30 | 13.45 |
Conclusion:
As can be seen from the above table: the adding of surfactant tween, so that active component gibberellin, 6-benzyl aminopurine decompose in water and slow down, through the optimization formula that has added tween being carried out the mensuration of the former medicine resolution ratio in heat storage front and back, the resolution ratio of two kinds of active ingredients all＜5%, meet the requirement of production, and the control group resolution ratio is much higher than the A group that has added tween.
The assay method of former medicine resolution ratio:
1. sample dissolves with mobile phase, take acetonitrile+0.1% glacial acetic acid aqueous solution as mobile phase, separates through silicagel column, adopts external standard method to carry out ultraviolet and quantitatively detects.
2. high performance liquid chromatography operating condition
Mobile phase: acetonitrile: 0.1% glacial acetic acid aqueous solution (V:V)=40:60;
Flow: 1.0ml/min;
Column temperature: room temperature;
Detect wavelength: gibberellic acid 207nm, 6-benzyl aminopurine 267nm;
Sampling volume: 5 μ L;
Retention time (min): gibberellic acid 2.9min, 6-benzyl aminopurine 9.7min.
3. the preparation of standard specimen solution
Take by weighing standard specimen 0.05g (being accurate to 0.0001g) in 100mL volumetric flask bottle, add mobile phase dissolving and be diluted to scale, place ultrasonic water bath to vibrate, with guarantee standard specimen dissolve fully, degassed, be cooled to room temperature, as mother liquor.To dilute successively with above-mentioned mother liquor the standard liquid of 5,10,25,50,100 times of preparations 100mg/L, 50mg/L, 20mg/L, 10mg/L, 5mg/L with mobile phase, shake up, cross 0.45 μ m film, sample introduction is set up respectively the calibration curve of gibberellic acid and 6-benzyl aminopurine.
4. the preparation of sample solution
Take by weighing sample 0.05g (being accurate to 0.0001g) in the 100mL volumetric flask, add mobile phase to scale, place ultrasonic water bath to vibrate, with guarantee sample dissolve fully, degassed, be cooled to room temperature, therefrom pipette 1mL in the 50mL volumetric flask with pipette, be diluted to scale with mobile phase, shake up, cross 0.45 μ m film, treat sample introduction.
5. measure
Under the aforesaid operations condition, after the instrument baseline stability, inject continuously number pin mark sample solution, calculate each pin relative response, the relative response for the treatment of adjacent two pins changes less than 1.5%, order according to standard specimen solution, sample solution, sample solution, standard specimen solution is measured, and each concentration is parallel advances three pins.
6. calculate
After the peak area of 6 concentration of standard specimen averaged respectively, take peak area as abscissa, concentration was that ordinate is mapped in the Excel form, sets up respectively the calibration curve regression equation of gibberellin and 6-benzyl aminopurine:
C1=0.107
A1-10.79?R2=0.9980…………………………………(1)
C2=0.0137
A2+0.6386?
R2=1.0000………………………………(2)
Peak area with gibberellin and 6-benzyl aminopurine
A1,
A2Substitution formula (1), formula (2) are calculated the concentration of the two respectively.In the formula:
A1---in the sample solution, the mean value of the former medicine peak area of gibberellin;
A2---in the sample solution, the mean value of the former medicine peak area of 6-benzyl aminopurine;
C1---in the sample solution, the concentration of the former medicine of gibberellin;
C2---in the sample solution, the concentration of the former medicine of 6-benzyl aminopurine.
The beneficial effect of embodiment 6 gibberellin, 6-benzyl aminopurine bi-component aqua
In order to obtain the effect of gibberellin, 6-benzyl aminopurine bi-component aqua, carry out following test:
To be used for bean sprout growth by the aqua of embodiment 1 preparation, and be made as the A group, control group does not add aqua, is made as the B group.
A organizes test method:
1. test material: press the aqua of embodiment 1 preparation, the bright mung bean of Tuquan
2. test method
(1) disinfecting utensils
The germination appliance of using in the test is sterilized with 84 thimerosals.
(2) medicament preparation
Aqua stoste is diluted to 11.1 mg/L, stand-by.
(3) mung bean preliminary treatment
Select full grains and glossiness beans, with choose bean or pea carry out weighing, take by weighing 15g.
(4) boiling hot kind and germination
Washed mung bean is scalded kind, soak 6h in the clear water.
To pour in the germination box through the kind beans that soak, then carry out trickle, every 6h drenches a water, and the trickle amount all is 100ml when noting guaranteeing each trickle.
(5) chemicals treatment
The 18h after mung bean seed soaking respectively, 36h, after spray the each 30ml of aqua after the dilution.
(6) data statistics
The data such as mung bean was cultivated five days after being transferred to the insulating box cultivation, in the incubation, some apparent indexs of bean sprouts was observed, and after cultivation finishes, added up root long, and bud is long, and hypocotyl is thick.
B organizes test method:
Do not add gibberellin, 6-benzyl aminopurine bi-component aqua in the test, other are organized with A.
Result of the test:
Organize respectively from A group, B, obtain at random 20 bean sprouts, the statistics root is long, canopy length, total length, and the data such as hypocotyl is thick, lateral root number are got its mean value, get table 3:
Conclusion:
As can be seen from the above table: aqua of the present invention is used for bean sprout growth, can grow by establishment bean sprouts fibrous root, reduces bean sprouts fibrous root quantity, shortens root long, and the bean sprouts is sturdy, improves quality.Obtain beneficial effect.
Embodiment 7 contains the bi-component aqua of 1.6% gibberellin and 1.2% 6-benzyl aminopurine-product residue research and risk assessment
One, experimental scheme
1. growth regulator: the bi-component aqua that contains 1.6% gibberellin and 1.2% 6-benzyl aminopurine
2. trial crops: moyashi, mung bean sprouts
3. test method: the production process of moyashi, mung bean sprouts is pressed embodiment 6, but has increased the aqua (54h after the seed soaking) that once sprays after the dilution.
Clear up dynamic experiment after last dispenser 0,0.5d, 1d, 1.5d, 2d, 3d gather the bean sprouts sample, measures respectively the wherein residual quantity of benayl aminopurine and gibberellic acid.
Final residue was tested bean sprouts harvest time, and bean sprout growth the 5th, 6,7 days be collecting sample respectively, measured respectively the wherein residual quantity of 6-benzyl aminopurine benzyl and gibberellin.
Two, detection method
1. instrument and equipment
Agilent 6410QQQ (Agilent Technologies, the U.S.).
2. the preparation of sample
The bean sprouts sample: every residential quarter random acquisition sample is stored in-20 ℃ of refrigerators to be analyzed after smashing to pieces.
3. analytical procedure
(1) extracts
Accurately take by weighing 10g sample (being accurate to 0.01g) in 50mL tool plug centrifuge tube, add the 5mL acetonitrile, vortex 1min extracts behind the jolting 30s, then extracting once with 5 mL acetonitriles, merge supernatant, add the anhydrous MgSO4 of 4g and 1g NaCl(and be accurate to 0.01g), violent jolting 10s is placed on and cools off 1～2min in the ice-water bath immediately, and then vortex 1min.Put into centrifuge, with the centrifugal 5min of 3800r/min rotating speed.Cross 0.22 μ m filter membrane to be measured in chromatogram auto injection bottle.
(2) analyze mensuration
A) instrument condition
Agilent 6410QQQ (Agilent Technologies, the U.S.).
Chromatographic column: zorbax-C18; Mobile phase: methyl alcohol: water (containing 0.1% formic acid)=70:30; Flow velocity: 0.3mL/min; Column temperature: 25 ℃; Sample size: 5 μ L.
The feature fragment ion:
6-benzyl aminopurine: m/z226.1, m/z 91(is quantitative), m/z 148;
Gibberellin: m/z345.1, m/z 143(is quantitative), m/z 239.
B) calibration curve
Accurately take by weighing benayl aminopurine, the gibberellic acid standard items are mixed with 1000mg/L acetonitrile stock solution, draw quantitative stock solution and join to get 5mg/L and 0.5mg/L standard operation solution with the dilution in acetonitrile constant volume, again with the blank extract series dilution of moyashi and mung bean sprouts join 0.005,0.01,0.05,0.1,0.5mg/L serial matrix standard liquid (benayl aminopurine), 0.05,0.1,0.5,1,2mg/L series matrix standard liquid (gibberellin) sample introduction 5uL, measure benayl aminopurine with above-mentioned chromatography-mass spectroscopy condition sample introduction, gibberellin concentration of standard solution and peak area are linear.Make calibration curve with benayl aminopurine, gibberellin concentration of standard solution and peak area.Linear equation sees Table 4:
C) limit of identification: under above-mentioned chromatography-mass spectroscopy condition, the limit of identification of benayl aminopurine, gibberellin is 0.25ng.
D) minimal detectable concentration: under above-mentioned chromatography-mass spectroscopy condition, the benayl aminopurine of method, gibberellic acid minimal detectable concentration: 0.05mg/kg.
E) relative retention time: under above-mentioned chromatography-mass spectroscopy condition, the relative retention time of benayl aminopurine, gibberellic acid is respectively 0.82, about 0.85min.
F) add the rate of recovery
Add benayl aminopurine, gibberellic acid standard specimen in blank sample, carry out respectively the interpolation recovery test of three concentration of 0.05,0.1,1mg/kg, each concentration repeats 5 times, extracts as stated above, purification and LC-MS-MS analyze.The average recovery rate of sample is 74.9～106.3%.The coefficient of variation is 0.8～11.8%, meets the Detecting Pesticide requirement.
(3) chart
Table 5 benayl aminopurine and gibberellic acid add the rate of recovery at moyashi and mung bean sprouts:
Table 6 benayl aminopurine and gibberellic acid clearing up dynamically in moyashi and mung bean sprouts:
Table 7: benayl aminopurine and the gibberellin residue test result in moyashi and mung bean sprouts:
(4) benayl aminopurine and gibberellic acid meals are taken in risk assessment in the bean sprouts
A) meals are taken in assessment
Long-term meals are taken in assessment according to the diet structure data in Ministry of Public Health's issue " Chinese different crowd consumption meals grouping recipe " or the coherent reference data, standard residue test intermediate value or established MRL (Maximum Residue Limit in conjunction with residual chemistry assessment recommendation, MRL), calculate estimation daily intake (the National Estimated Daily Intake of country, NEDI), the acceptable daily intake with toxicology assessment recommendation compares.
The short-term meals are taken in assessment according to the diet structure data in Ministry of Public Health's issue " Chinese different crowd consumption meals grouping recipe " or the coherent reference data, high residue value or the established MRL (MRLs) recommended in conjunction with residual chemistry assessment, calculate estimation short-term intake (the National Estimated ShortTerm Intake of country, NESTI), the acute reference dose with toxicology assessment recommendation compares.
Take in assessment result according to toxicology, residual chemistry and meals, determine acceptable daily intake (ADI), acute reference dose (ARfD) and residue test intermediate value (STMR) and high residue value (HR), and recommend maximum residue limit value or risk management suggestion to risk management function.
B) population of China is 15g/ (dayperson) for the intake investigation result of bean sprouts.According to above standard residue test final residue quantitative statistics.Benayl aminopurine and gibberellic acid are that the residue test intermediate value (STMR) of the low concentration dispenser 2 times of experimental scheme is respectively 0.117 and 0.142 according to GAP on moyashi; High residue value (HR) is respectively 0.137 and 0.177; Residue test intermediate value (STMR) on mung bean sprouts is respectively 0.092 and 0.029; High residue value (HR) is respectively 0.121 and 0.041.Benayl aminopurine and gibberellic acid are that the residue test intermediate value (STMR) of the low concentration of experimental scheme and high concentration dispenser 2 times and 3 times is respectively 0.123 and 0.169 according to critical GAP on moyashi; High residue value (HR) is respectively 0.146 and 0.281; Residue test intermediate value (STMR) on mung bean sprouts is respectively 0.083 and 0.043; High residue value (HR) is respectively 0.121 and 0.046.
C) the ADI value of benayl aminopurine and gibberellin is respectively 0.05mg (kgbwd) and 3 mg/ (kgbwd).The MRL value of China's benayl aminopurine and gibberellin is respectively 0.2mg/kg and 0.5mg/kg.The residual chronic risk assessment of benayl aminopurine and gibberellin sees Table 8 and table 9 in the bean sprouts.Different chronic risk merchant RQc and acute risk merchant RQa are all＜100%.Show that it is acceptable that meals are taken in risk.
The residual chronic risk assessment of benayl aminopurine and gibberellic acid in table 8 bean sprouts:
Table 9
The residual acute risk assessment of benayl aminopurine and gibberellic acid in the bean sprouts:
Conclusion: carry out risk assessment with the Cmax 11.1mg/L that uses in the aqua of the present invention, prove that with production of the present invention bean sprouts method and aqua be very safe.
Claims (3)
1. aqua that is used for the growth of beans bud dish is characterized in that: in the aqua prescription each component by percentage to the quality, consumption is respectively:
Raw material form consumption
Gibberellin 1.6
6-benzyladenine 1.16-1.24
Acetic acid 25.0
Ethanol 15.0
Tween 4.8-5.2
Running water polishing to 100.
2. described aqua for beans bud dish growth according to claim 1, its preparation method is as follows:
Prescription: in the aqua prescription each component by percentage to the quality, consumption is respectively:
Raw material form consumption
Gibberellin 1.6
6-benzyladenine 1.16～1.24
Acetic acid 25.0
Ethanol 15.0
Tween 4.8～5.2
Running water polishing to 100;
Preparation technology:
(1) in stainless steel cask, add 1.6 parts of gibberellin, 15.0 parts of ethanol, stirring and dissolving, solution enters material-compound tank;
(2) in stainless steel cask, add 1.16～1.24 parts of 6-benzyladenines, 25.0 parts of acetic acid, stirring and dissolving, solution enters material-compound tank;
(3) in material-compound tank, add 4.8～5.2 parts of tweens, the running water polishing stirred 60 minutes to 100 parts of solution total amounts;
(4) filtering solution;
(5) check filtrate is filled into filtrate in infusion bottle or the bag;
(6) packing.
3. the aqua for beans bud dish growth according to claim 1, its active component detects with high performance liquid chromatography, and operating condition is as follows:
Mobile phase: in volume ratio, acetonitrile: 0.1% glacial acetic acid aqueous solution=40:60;
Flow: 1.0ml/min;
Column temperature: room temperature;
Detect wavelength: gibberellin 207nm, 6-benzyl aminopurine 267nm;
Sampling volume: 5 μ L;
Retention time: gibberellin 2.9min, 6-benzyl aminopurine 9.7min.
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