CN102758289A - Woven fabric carrier for cell immobilization and preparation method thereof - Google Patents
Woven fabric carrier for cell immobilization and preparation method thereof Download PDFInfo
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- CN102758289A CN102758289A CN2012102762138A CN201210276213A CN102758289A CN 102758289 A CN102758289 A CN 102758289A CN 2012102762138 A CN2012102762138 A CN 2012102762138A CN 201210276213 A CN201210276213 A CN 201210276213A CN 102758289 A CN102758289 A CN 102758289A
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Abstract
The invention discloses a woven fabric carrier for cell immobilization and a preparation method thereof and belongs to the field of biological engineering. The preparation method of the woven fabric carrier comprises selecting a fiber from one of dacron, nylon, cotton and hemp; designing a fabric weave to enable the fabric weave to be a compound double-layer weave of random two of a basket weave, a honeycomb weave, a through hole weave and a crepe weave; selecting weaving parameters; and performing postprocessing, using a sewing or bundling method to process a woven fabric discharged out of a machine into a cylinder with the diameter of 2-7cm, and reserving after sterilization. The woven fabric carrier is used for immobilization of terephthalic acid (TA) degradation bacterium testosterone comamonas, and the degradation rate of the TA is greatly improved in comparison with a control sample which is not immobilized.
Description
Technical field
The invention belongs to bioengineering field, relate to a kind of preparation method of woven fabric carrier, be specifically related to a kind of preparation method who is used for the woven fabric carrier of cell fixation.
Background technology
Immobilized cell technique is meant and utilizes physics or chemical means free cell to be positioned specific area of space and to make it keep active a kind of technology.At present, immobilized cell technique develops rapidly becomes one of research focus in the bioengineering, and it uses a plurality of fields such as food, medicine, chemical industry, the energy, environment that related to.
The selection of carrier is the key of immobilized cell technique.The deficiency of carrier at aspects such as biocompatibility, mass-transfer performance, mechanical strength, processing and moulding, cost and service lifes that is used for cell fixation at present restricting further developing of immobilized cell technique, the bottleneck that becomes the immobilized cell technique development and use.Just need to seek and design novel carrier and method for immobilizing cell for addressing this problem.
The space of textiles can make Premeabilisation of cells go into, and forms very big cell colony therein to realize cell fixation.Textiles has that specific area is big, mass-transfer performance is good, be easy to process and cheap advantage, aspect cell immobilization carrier, has great potentiality.But the cell immobilization carrier material that adopts at present mainly is three types of organic polymer carrier, inorganic carrier and complex carriers, and used for textiles is also rare in the research of cell fixation.
Compare with other carrier, have following advantage with textiles as the immobilization that carrier carries out cell: (1) textiles has variform, comprises bulk fibre, yarn, fabric (woven fabric, knitted fabric, non-weaving cloth), to satisfy the needs of different situations; (2) textile carrier exists as aggregate of fibers, because the draw ratio of fiber is big, thereby textile carrier has bigger surface area, is beneficial to the carrying out of catalytic reaction; (3) compare with pelleted substrate, the textile carrier structure is more loose, more helps the transmission of material and separating of product; (4) textile carrier has excellent mechanical intensity, chemical stability and biocompatibility; (5) textile carrier has good processing properties, can carry out the from the beginning design that begins from fiber, and textile carrier can carry out suitability for industrialized production as a kind of of textiles; (6) textile fabric suitability for industrialized production is easy to obtain low price.
Summary of the invention
The object of the present invention is to provide a kind of woven fabric carrier that is used for cell fixation, this woven fabric carrier specific area is big, mass-transfer performance good, be easy to processing and cheap, is suitable in biological product preparation and pollutant biodegradation, promoting the use of.
Technical scheme provided by the invention is following:
The described woven fabric carrier that is used for cell fixation can be prepared by following method:
(1) selection of fiber
According to mechanical strength, chemical stability and the biocompatibility characteristics of fiber, select suitable fiber in order to the woven textiles carrier;
Said fiber is selected a kind of in terylene, polyamide fibre, acrylic fibers, cotton, the fiber crops;
(2) design of fabric tissue
Said Dobby Weave Design is any two kinds of compound double-layered structures in basket weave, honeycomb weave, mock leno weave, the crape tissue;
(3) weave parameters of choice
Said Fabrication parameter is: warp count is that 280～460/10cm, weft count are that 150～360/10cm, every reed penetrate that number is 1～4, porter is 60～200;
(4) back processing
It is the cylindric of 2～7cm that woven fabric behind the following machine uses the method for making or tying up to be processed into diameter, after the sterilization, subsequent use.
The preparation method of the described woven fabric carrier that is used for cell fixation is following:
(1) selection of fiber
According to mechanical strength, chemical stability and the biocompatibility characteristics of fiber, select suitable fiber in order to the woven textiles carrier;
Said fiber is selected a kind of in terylene, polyamide fibre, acrylic fibers, cotton, the fiber crops;
(2) design of fabric tissue
Said Dobby Weave Design is any two kinds of compound double-layered structures in basket weave, honeycomb weave, mock leno weave, the crape tissue;
(3) weave parameters of choice
Said Fabrication parameter is: warp count is that 280～460/10cm, weft count are that 150～360/10cm, every reed penetrate that number is 1～4, porter is 60～200;
(4) back processing
It is the cylindric of 2～7cm that woven fabric behind the following machine uses the method for making or tying up to be processed into diameter, after the sterilization, subsequent use.
Beneficial effect
Woven fabric carrier of the present invention is used for the immobilization of terephthalic acid (TPA) (TA) degradation bacteria Comamonas testosteroni, and does not carry out immobilized control samples relatively, the degradation rate of TA improves a lot.After cultivating 4h; The degradation rate of TA is than the raising 27% of not carrying out the cell fixation sample in the cell fixation sample; After cultivating 8h; The degradation rate of TA is than the raising 45% of not carrying out the cell fixation sample in the cell fixation sample, and after cultivating 12h, the degradation rate of TA is than the raising 42% of not carrying out the cell fixation sample in the cell fixation sample.
The specific embodiment
Below through concrete embodiment narration the inventive method.In addition, embodiment is interpreted as illustrative, and unrestricted scope of the present invention, essence of the present invention and scope are only limited claims.To those skilled in the art, under the prerequisite that does not deviate from essence of the present invention and scope, various changes or change that the crystallization condition in these embodiments is carried out also belong to protection scope of the present invention.
Embodiment 1
Fiber is selected polyester filament for use; The yarn model is that 40/2 English props up; Dobby Weave Design is mock leno weave and the compound double-layered structure of honeycomb weave, and warp count is that 400/10cm, weft count are that 350/10cm, every reed penetrate that number is 3, porter is 100, and it is the cylindric of 3cm that the woven fabric behind the following machine is sewn into diameter; After the sterilization, subsequent use.
Embodiment 2
Fiber is selected the cotton yarn of 14 * 2tex for use; Dobby Weave Design is basket weave and the compound double-layered structure of honeycomb weave; Warp count is that 432/10cm, weft count are that 250/10cm, every reed penetrate that number is 4, porter is 108; It is the cylindric of 3cm that woven fabric behind the following machine is sewn into diameter, after the sterilization, subsequent use.
Embodiment 3
The polyester staple fiber yarn that fiber selects for use 40/2 English to prop up; Dobby Weave Design is that mock leno weave and crape are organized compound double-layered structure; Warp count is that 400/10cm, weft count are that 300/10cm, every reed penetrate that number is 3, porter is 100, and it is the cylindric of 5cm that the woven fabric behind the following machine is rolled into diameter, ties up; After the sterilization, subsequent use.
Claims (5)
1. a woven fabric carrier that is used for cell fixation is characterized in that, can be prepared by following method:
(1) selection of fiber
According to mechanical strength, chemical stability and the biocompatibility characteristics of fiber, select suitable fiber in order to the woven textiles carrier; Said fiber is selected a kind of in terylene, polyamide fibre, acrylic fibers, cotton, the fiber crops;
(2) design of fabric tissue
Said Dobby Weave Design is any two kinds of compound double-layered structures in basket weave, honeycomb weave, mock leno weave, the crape tissue;
(3) weave parameters of choice
Said Fabrication parameter is: warp count is that 280～460/10cm, weft count are that 150～360/10cm, every reed penetrate that number is 1～4, porter is 60～200;
(4) back processing
It is the cylindric of 2～7cm that woven fabric behind the following machine uses the method for making or tying up to be processed into diameter, after the sterilization, subsequent use.
2. preparation method who is used for the woven fabric carrier of cell fixation according to claim 1, step is following:
(1) selection of fiber
According to mechanical strength, chemical stability and the biocompatibility characteristics of fiber, select suitable fiber in order to the woven textiles carrier; Said fiber is selected a kind of in terylene, polyamide fibre, acrylic fibers, cotton, the fiber crops;
(2) design of fabric tissue
Said Dobby Weave Design is any two kinds of compound double-layered structures in basket weave, honeycomb weave, mock leno weave, the crape tissue;
(3) weave parameters of choice
Said Fabrication parameter is: warp count is that 280～460/10cm, weft count are that 150～360/10cm, every reed penetrate that number is 1～4, porter is 60～200;
(4) back processing
It is the cylindric of 2～7cm that woven fabric behind the following machine uses the method for making or tying up to be processed into diameter, after the sterilization, subsequent use.
3. a kind of woven fabric carrier that is used for cell fixation according to claim 1 is characterized in that said preparation method is following: fiber is selected polyester filament for use; The yarn model is that 40/2 English props up; Dobby Weave Design is mock leno weave and the compound double-layered structure of honeycomb weave, and warp count is that 400/10cm, weft count are that 350/10cm, every reed penetrate that number is 3, porter is 100, and it is the cylindric of 3cm that the woven fabric behind the following machine is sewn into diameter; After the sterilization, subsequent use.
4. a kind of woven fabric carrier that is used for cell fixation according to claim 1; It is characterized in that said preparation method is following: fiber is selected the cotton yarn of 14 * 2tex for use, and Dobby Weave Design is basket weave and the compound double-layered structure of honeycomb weave; Warp count is that 432/10cm, weft count are that 250/10cm, every reed penetrate that number is 4, porter is 108; It is the cylindric of 3cm that woven fabric behind the following machine is sewn into diameter, after the sterilization, subsequent use.
5. a kind of woven fabric carrier that is used for cell fixation according to claim 1 is characterized in that said preparation method is following: the polyester staple fiber yarn that fiber selects for use 40/2 English to prop up; Dobby Weave Design is that mock leno weave and crape are organized compound double-layered structure; Warp count is that 400/10cm, weft count are that 300/10cm, every reed penetrate that number is 3, porter is 100, and it is the cylindric of 5cm that the woven fabric behind the following machine is rolled into diameter, ties up; After the sterilization, subsequent use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102762138A CN102758289A (en) | 2012-08-06 | 2012-08-06 | Woven fabric carrier for cell immobilization and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102762138A CN102758289A (en) | 2012-08-06 | 2012-08-06 | Woven fabric carrier for cell immobilization and preparation method thereof |
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| CN102758289A true CN102758289A (en) | 2012-10-31 |
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| CN2012102762138A Pending CN102758289A (en) | 2012-08-06 | 2012-08-06 | Woven fabric carrier for cell immobilization and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021188405A1 (en) * | 2020-03-18 | 2021-09-23 | Edwards Lifesciences Corporation | Textiles, implantable medical devices using such textiles, and processes for making the same |
Citations (7)
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|---|---|---|---|---|
| WO1989000195A1 (en) * | 1987-07-01 | 1989-01-12 | Novo Industri A/S | Process for immobilization of biological material by cross-linking with polyazetidine |
| CN2132750Y (en) * | 1992-09-02 | 1993-05-12 | 江苏省食品发酵研究所 | Film carrier of fixed microbial cell and enzyme |
| JP3219305B2 (en) * | 1991-05-17 | 2001-10-15 | 日本バイリーン株式会社 | Carrier for immobilizing animal cells and cell culture method using the same |
| EP1188821A1 (en) * | 2000-03-31 | 2002-03-20 | National Agricultural Research Organisation | Carrier for animal cell culture comprising organ tissue slice and animal cell culture method and transplantation method with the use of the carrier |
| US20020064875A1 (en) * | 2000-10-06 | 2002-05-30 | Industrial Technology Research Institute | Carrier for cell attachment or fixation and its process for preparation |
| CN101613690A (en) * | 2008-06-27 | 2009-12-30 | 北京化工大学 | A kind of lipase immobilization method |
| US20100184183A1 (en) * | 2007-06-13 | 2010-07-22 | Olivier Schussler | Collagen scaffold modified by covalent grafting of adhesion molecules, associated methods and use thereof for cardiovascular and thoracic cell therapy and contractile tissue engineering |
-
2012
- 2012-08-06 CN CN2012102762138A patent/CN102758289A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989000195A1 (en) * | 1987-07-01 | 1989-01-12 | Novo Industri A/S | Process for immobilization of biological material by cross-linking with polyazetidine |
| JP3219305B2 (en) * | 1991-05-17 | 2001-10-15 | 日本バイリーン株式会社 | Carrier for immobilizing animal cells and cell culture method using the same |
| CN2132750Y (en) * | 1992-09-02 | 1993-05-12 | 江苏省食品发酵研究所 | Film carrier of fixed microbial cell and enzyme |
| EP1188821A1 (en) * | 2000-03-31 | 2002-03-20 | National Agricultural Research Organisation | Carrier for animal cell culture comprising organ tissue slice and animal cell culture method and transplantation method with the use of the carrier |
| US20020064875A1 (en) * | 2000-10-06 | 2002-05-30 | Industrial Technology Research Institute | Carrier for cell attachment or fixation and its process for preparation |
| US20100184183A1 (en) * | 2007-06-13 | 2010-07-22 | Olivier Schussler | Collagen scaffold modified by covalent grafting of adhesion molecules, associated methods and use thereof for cardiovascular and thoracic cell therapy and contractile tissue engineering |
| CN101613690A (en) * | 2008-06-27 | 2009-12-30 | 北京化工大学 | A kind of lipase immobilization method |
Non-Patent Citations (1)
| Title |
|---|
| 蔡陛霞: "《织物结构与设计》", 31 May 2008, article "第4章", pages: 196-198 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021188405A1 (en) * | 2020-03-18 | 2021-09-23 | Edwards Lifesciences Corporation | Textiles, implantable medical devices using such textiles, and processes for making the same |
| CN115485426A (en) * | 2020-03-18 | 2022-12-16 | 爱德华兹生命科学公司 | Textile product, implantable medical device using such a textile product and process for manufacturing such a textile product |
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Application publication date: 20121031 |