CN102757895A - Diatom growth variable research system based on circular diatom cultivation shelf - Google Patents
Diatom growth variable research system based on circular diatom cultivation shelf Download PDFInfo
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- CN102757895A CN102757895A CN201210282067XA CN201210282067A CN102757895A CN 102757895 A CN102757895 A CN 102757895A CN 201210282067X A CN201210282067X A CN 201210282067XA CN 201210282067 A CN201210282067 A CN 201210282067A CN 102757895 A CN102757895 A CN 102757895A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The invention discloses a diatom growth variable research system based on a circular diatom cultivation shelf. The research system is characterized by comprising a cultivation chamber. An air conditioner and a circular diatom cultivation shelf are arranged in the cultivation chamber. A plurality of incandescent lamp sources are vertically distributed on the outer surface of the circular diatom cultivation shelf, and a supporting plate is horizontally arranged on the bottom portion on the outer side of the circular diatom cultivation shelf. A plurality of container locating grooves are arranged on the supporting plate, and the plurality of the container locating grooves are arranged into multiple lines in the radial direction along the diameter direction of the horizontal section circle of the circular diatom cultivation shelf where the plurality of the incandescent lamp sources are placed, and multiple circles having a same center with the horizontal section circle of the circular diatom cultivation shelf in the circumferential direction. The research system further comprises a color control mechanism. According to the diatom growth variable research system based on the circular diatom cultivation shelf, variables affecting the diatom growth can be adjusted and controlled, so that researches of influences of variables on the diatom growth are facilitated, and the research system has the advantages of being simple in structure, and convenient and rapid to adjust and control variables.
Description
Technical field
The invention belongs to diatom and culture studying technological domain, be specifically related to a kind of growth of diatom algae Variable Study system based on circular diatom culturing rack.
Background technology
In the failure mode of part, surface disturbance lost efficacy and to occupy very big proportion, and people attempt all respects such as the design, processing, selection from part and start with and reduce the generation of this fatigue, but all can not obtain good effect.At present; Bionical manufacturing technology has been widely used in mechanical industry; Function through research and mimic biology body forms mechanism; Design and manufacturing and biological calligraphy or painting model have material, structure, device and the equipment of similar characteristics, and biological motion executive system, sensory perceptual system, system, breaking system and specific function structure etc. are duplicated.Natural biology is through the evolution of For hundreds of millions of years; The excellent performance that forms in motor system (mechanism), perception, driving, control and part specific function structure aspects is that most Prosthesises are incomparable, and this bionical manufacturing research to high-performance mechanism, device and equipment provides condition.
Diatom is one type of small unicellular algae, has the sclereid wall of being made up of amorphous silicon oxide (silicon shell), and diatom test has exquisite form and structure.Find through measuring; Diatom test can bear the order of magnitude of pressure at
Pa; Therefore can under rugged environment, keep vigorous vitality, never eliminated by the Nature.Copy the substruction of diatom, simulate its living environment, the research mechanical property is explored best basic resulting structure and critical dimension thus, and is applied to go in the manufacturing and designing of the little processing of piece surface or little apparatus.This research is for the performance of improving part or apparatus, reduce because part failure and the loss that brings to people, and to future mechanical industry development wide prospect and profound significance are arranged.
Common diatom is cultured its purpose and is to obtain food, medicine, makeup, polymer materials, nutrition-fortifying agent, microbial inoculum etc.; Breeding environment does not add special restriction yet; This breeding way can't learn that obviously the microstructure of diatom which kind of has taken place changed in the process of cultivating, also can't simulate the influence of varying environment variable to the growth of diatom algae situation.
Summary of the invention
To the problems referred to above and deficiency; Technical problem to be solved by this invention is: how a kind of growth of diatom algae Variable Study system based on circular diatom culturing rack that can simulate and regulate the various variable situation of growth of diatom algae is provided, with the influence performance of convenient each variable of research to growth of diatom algae.
In order to address the above problem, the technical scheme below the present invention has adopted.
A kind of growth of diatom algae Variable Study system based on circular diatom culturing rack; It is characterized in that; Comprise culturing room; Culturing room has the air-conditioning that is used to control room temp, also is provided with circular diatom culturing rack in the culturing room, and said circular diatom culturing rack is the vertical round shape that is provided with; Circular diatom culturing rack outside surface also vertically is uniformly distributed with and is provided with many incandescent light source; Also be provided with the energising duration trip switch that is used for controlling simultaneously said many light sources, said circular diatom culturing rack exterior bottom has been horizontally disposed with supporting plate, and supporting plate is provided with some vessel detents; Said some vessel detents are arranged as along the multiple row of the circular diatom culturing rack horizontal section circular diameter direction at every incandescent light source place diametrically, are arranged as the many circles that have together circle center with circular diatom culturing rack horizontal section circle simultaneously in a circumferential direction; Also include the color member; Said color member comprises a plurality of rollers that vertically are arranged at the circular diatom culturing rack outside; Roller to the radial distance in the center of circle, circular diatom culturing rack horizontal section greater than light source to the center of circle, circular diatom culturing rack horizontal section radial distance; Roller comprises two the main roller and the some secondary rollers that on end face, have handle; Two adjacent settings of main roller also are provided with colorful film, and said colorful film one end is wrapped on the main roller; The other end passes through said secondary roller successively and curls up a Zhou Houzai along circular diatom culturing rack circumferential direction and is wrapped on another root master roller, and said colorful film has the color section of several different colours.
When this research system uses; The diatom that diatom is housed is cultivated vessel to be placed in each vessel detent; Can the analog temperature variable and temperature variable carried out regulating and controlling through air-conditioning; Through incandescent light source simulated light photograph, make the illumination color that appears film can regulate control through the control colorful film simultaneously, can regulate control to light application time through energising duration trip switch; The structure setting of vessel detent; It is identical to have guaranteed that diatom on each circumference is cultivated the suffered intensity of illumination of vessel, and to cultivate the suffered intensity of illumination of vessel from circular diatom culturing rack diatom far away more low more and radially go up, so can simulate the situation of different illumination intensity.When so native system uses, can be to temperature, these several growth of diatom algae variablees of intensity of illumination and illumination color are regulated control, compare test, just can study the influence of each variable to the growth of diatom algae situation.Native system structure simultaneously, possess simple in structure, the very convenient advantage efficiently of adjusting control of each variable.
In sum, system of the present invention can regulate control to the various variablees that influence growth of diatom algae, with the influence situation of convenient each variable of research to growth of diatom algae, possess simultaneously simple in structure, to the adjusting of variable with control very convenient advantage efficiently.
Figure of description
The structural representation of the circular diatom culturing rack that adopts when Fig. 1 implements for native system.
Fig. 2 is the vertical view of Fig. 1.
Embodiment
During practical implementation; A kind of growth of diatom algae Variable Study system based on circular diatom culturing rack wherein, comprises culturing room; Culturing room has the air-conditioning that is used to control room temp; Also be provided with circular diatom culturing rack in the culturing room, referring to Fig. 1 and Fig. 2, said circular diatom culturing rack is the vertical round shape that is provided with; The outside surface of circular diatom culturing rack also vertically is uniformly distributed with and is provided with many incandescent light source 2; Also be provided with the energising duration trip switch (place not shown in the figures) that is used for controlling simultaneously said many light sources, said circular diatom culturing rack exterior bottom has been horizontally disposed with supporting plate 4, and supporting plate 4 is provided with some vessel detents 3; Said some vessel detents 3 are arranged as along the multiple row of the circular diatom culturing rack horizontal section circular diameter direction at every incandescent light source place diametrically, are arranged as the many circles that have together circle center with circular diatom culturing rack horizontal section circle simultaneously in a circumferential direction; Also include the color member; Said color member comprises a plurality of rollers that vertically are arranged at the circular diatom culturing rack outside; Roller comprises the main roller 6 and some secondary rollers 1 of band rotating handles on two end faces; Roller to the radial distance in the center of circle, circular diatom culturing rack horizontal section greater than light source to the center of circle, circular diatom culturing rack horizontal section radial distance; Wherein two adjacent settings of main roller are twined on it and are provided with the colorful film (not shown), and colorful film one end is wrapped on the main roller; The other end passes through said secondary roller successively and curls up a Zhou Houzai along circular diatom culturing rack circumferential direction and is wrapped on another root master roller, and said colorful film has the color section of several different colours.
In addition, during practical implementation, in circular diatom culturing rack bottom wiring can also be set and place chamber 5, be used for wiring and place circuit module, make wiring be unlikely in disorder.
When native system uses; Lean on air-conditioning that temperature variable is regulated control; Lean on the different positions far and near of vessel detent 3 to control to looking after intensity apart from light source; By energising duration trip switch light irradiation time is regulated control, by rotating main roller and then rotating colorful film the illumination color that appears film is regulated control.So native system can be regulated control to four variablees simultaneously,, just can study the influence that respectively diatom is produced under the situation of one or more variablees changes easily through simultaneous test.
Adopt concrete verification experimental verification feasibility of the present invention below.
For making things convenient for the contrast experiment; With the diatom note work group A that cultivates in the circular diatom culturing rack; The diatom note work group B (the B group is reference group) that cultivates in the natural light; Every group has 5 kinds of diatoms (being respectively chlorella, the big flat algae in Qingdao, box-like algae, rotary strainer algae A, rotary strainer algae B); Wherein the temperature-controlled process of reference group group B is identical with experimental group A, and intensity of illumination then is to control through the distance (supposition is 0mm near glass) of regulate cultivating the natural light that beaker and window inject, and the illumination color then can realize through the light transmission film of stickup different colours on window-glass.
During practical implementation, can formulate following culture scheme:
(1) basic setup
1), nutrient solution preparation
The diatom nutrient solution is in strict accordance with " ' f/2 ' nutrient solution original formulation " (this prescription can obtain from open resource) preparation.
2), protect kind of a condition
Test exhaustless diatom germplasm and should be placed on guarantor's kind under the following condition: 20 ℃, intensity of illumination 2500-3000lux, periodicity of illumination 12h/d, medium pH 7.8-8.0.
3), the diatom germplasm distributes
The diatom germplasm is buied from silicon ocean institute of the Chinese Academy of Sciences; Each diatom germplasm volume is 100ml, and " f/2 " nutrient solution original formulation solution and diatom germplasm volume proportion 1000ml:100ml place top 2 with 5 kinds of diatom germplasms after the dilution) cultivate under the said condition after 30 days; Continue to add " f/2 " nutrient solution original formulation solution dilution to satisfy the required diatom of a large amount of experiment contrasts; When diatom is cultivated,, and refresh the water periodically its unified being put in the 250ml beaker.
4), light control
Control light irradiation time through energising duration trip switch, the light application time of switching on every day section is 10:00-22:00, and the carton overspread time period is 22:00-10:00.
5), change water with take pictures, the microscopic examination cycle
Exchange water cycle is 10 days, takes pictures and the microscopic examination cycle is 20 days, the content that will note when taking pictures: the outside drawing of taking each diatom; Notice that camera is parallel with horizontal plane; The vertical shooting lets beaker at utmost get into the camera image display, and on picture, indicates shooting time; Specific to every day, form: " Time:2012-07-15 ".
(2) contrast solution formulation and embodiment thereof
The variable controlled variable of native system is temperature, illumination color, intensity of illumination, when considering the influencing of a certain variable, needs strict its dependent variable of control to be consistent, below in the form data recorded be the specific growth rate (d of unit of various diatoms
-1).
1), observe the influence of differing temps to growth of diatom algae, control illumination color is a white light, intensity of illumination is 100mm, draws record form such as following table 1
2), observe of the influence of different light color to growth of diatom algae, controlled temperature is 20 ℃, intensity of illumination is 100mm, draws record form such as following table 2
3), observe the influence of different illumination intensity to growth of diatom algae, controlled temperature is 20 ℃, the illumination color be a white, drafting record form such as following table 3
In order to verify effect of the present invention; The applicant has carried out microscopic examination according to the predetermined microscopic examination cycle to various diatoms; As time goes on the result finds and the variation of environmental variance that the specific growth rate of various diatoms all has different the variation, and delta data is listed in above table; Prove that thus the present invention can find the influence performance of various variablees to the growth of diatom algae situation really, cultivating for diatom from now on provides empirical suggestion.
Claims (1)
1. growth of diatom algae Variable Study system based on circular diatom culturing rack; It is characterized in that: comprise culturing room; Culturing room has the air-conditioning that is used to control room temp; Also be provided with circular diatom culturing rack in the culturing room; Said circular diatom culturing rack is the vertical round shape that is provided with, and circular diatom culturing rack outside surface also vertically is uniformly distributed with and is provided with many incandescent light source, also is provided with the energising duration trip switch that is used for controlling simultaneously said many light sources; Said circular diatom culturing rack exterior bottom has been horizontally disposed with supporting plate; Supporting plate is provided with some vessel detents, and said some vessel detents are arranged as along the multiple row of the circular diatom culturing rack horizontal section circular diameter direction at every incandescent light source place diametrically, are arranged as the many circles that have together circle center with circular diatom culturing rack horizontal section circle simultaneously in a circumferential direction; Also include the color member; Said color member comprises a plurality of rollers that vertically are arranged at the circular diatom culturing rack outside; Roller to the radial distance in the center of circle, circular diatom culturing rack horizontal section greater than light source to the center of circle, circular diatom culturing rack horizontal section radial distance; Roller comprises two the main roller and the some secondary rollers that on end face, have handle; Two adjacent settings of main roller also are provided with colorful film, and said colorful film one end is wrapped on the main roller; The other end passes through said secondary roller successively and curls up a Zhou Houzai along circular diatom culturing rack circumferential direction and is wrapped on another root master roller, and said colorful film has the color section of several different colours.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210282067.XA CN102757895B (en) | 2012-08-09 | 2012-08-09 | Diatom growth variable research system based on circular diatom cultivation shelf |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210282067.XA CN102757895B (en) | 2012-08-09 | 2012-08-09 | Diatom growth variable research system based on circular diatom cultivation shelf |
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| CN102757895A true CN102757895A (en) | 2012-10-31 |
| CN102757895B CN102757895B (en) | 2014-03-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838585A (en) * | 2016-06-01 | 2016-08-10 | 海南大学 | Device and method for cultivating diatom |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2053760U (en) * | 1989-04-19 | 1990-02-28 | 天津师范大学 | Hf-1 model color-light transparent membrane products |
| JP2003010697A (en) * | 2000-12-28 | 2003-01-14 | Nok Corp | Photocatalyst structure and photocatalyst reactor |
| CN101613664A (en) * | 2009-07-16 | 2009-12-30 | 青岛生物能源与过程研究所 | A kind of airlift photobioreactor |
| CN102325868A (en) * | 2008-12-23 | 2012-01-18 | Xtu公司 | Device for cultivating algae and/or microorganisms for treating an effluent, and biological frontage |
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2012
- 2012-08-09 CN CN201210282067.XA patent/CN102757895B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2053760U (en) * | 1989-04-19 | 1990-02-28 | 天津师范大学 | Hf-1 model color-light transparent membrane products |
| JP2003010697A (en) * | 2000-12-28 | 2003-01-14 | Nok Corp | Photocatalyst structure and photocatalyst reactor |
| CN102325868A (en) * | 2008-12-23 | 2012-01-18 | Xtu公司 | Device for cultivating algae and/or microorganisms for treating an effluent, and biological frontage |
| CN101613664A (en) * | 2009-07-16 | 2009-12-30 | 青岛生物能源与过程研究所 | A kind of airlift photobioreactor |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838585A (en) * | 2016-06-01 | 2016-08-10 | 海南大学 | Device and method for cultivating diatom |
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