CN102749457A

CN102749457A - Beta2-microglobulin detection kit and preparing method thereof

Info

Publication number: CN102749457A
Application number: CN2012102618404A
Authority: CN
Inventors: 张雷
Original assignee: BEIJING ENJIHE BIOTECHNOLOGY Co Ltd
Current assignee: BEIJING ENJIHE BIOTECHNOLOGY Co Ltd
Priority date: 2012-07-27
Filing date: 2012-07-27
Publication date: 2012-10-24

Abstract

A beta2-microglobulin detection kit contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared by buffer solution, polyethylene glycol, sodium azide, edetic acid and bovine serum albumin, and the reagent R2 is prepared by buffer solution, emulsion particles combined with beta2-microglobulin monoclonal antibodies, polysorbate-20, sodium azide, edetic acid and bovine serum albumin. Samples and the reagents are mixed according to a certain volume ratio and led to have a series of reactions, then reactants are placed under a semi-automatic/full-automatic biochemical analyzer, absorbance change speed of the reactants at the position with dominant wavelength as 550nm is detected, and accordingly concentration of beta2-microglobulin is measured and calculated. The kit has the advantages of being accurate, stable and convenient.

Description

A kind of B2M detection kit and preparation method thereof

Technical field

The invention belongs to the biological medicine technology field, relate in particular to a kind of detection kit of the people's of detection B2M.

Background technology

The single chain polypeptide low molecular protein that B2M is made up of 100 amino acid residues.Electrophoresis is in β 2 districts, so claim B2M.Originate from people's plastid stroma, the normal cell of epithelial cell and hemopoietic system and malignant cell all can be synthesized B2M.

β 2-microglobulin relative molecular weight is little; Can freely pass through glomerular filtration; And, do not receive what the influence of age, sex, body musculature, so serum 2-microglobulin is the early stage index of renal damage only by producing constant rate in RE and the body.

The rising of serum 2-microglobulin can reflect the situation that the glomerular filtration function is impaired or whether filtered load increases, and increases and discharge β 2-microglobulin in the urine, then points out tubular injury or filtered load to increase.The blood B2M raises and the urine B2M is normal, mainly due to the glomerular filtration function reduction, is common in acute and chronic ephritis, renal failure etc.

Serum 2-microglobulin increases, and except that reducing because of glomerular filtration rate(GFR, Another reason is synthetic increasing; Being shown in the neoplasm venereal disease becomes and the connective tissue pathology; Aspect tumour, in the pathology that the dissemination pathology is limited to, more be prone to produce serum 2-microglobulin and raise.Some malignant tumour (like primary carcinoma of liver, lung cancer, myeloma etc.), during autoimmune disease (like systemic loupus erythematosus, hemolytic anemia), blood, urine B2M all raise.

Therefore, B2M is determined at and estimates renal function, assists diagnosis and differential diagnosis malignant tumour aspect that remarkable effect is arranged.

The method of at present known mensuration β 2-microglobulin has SRID, IE, radioimmunology, ELISA.These methods have the shortcoming of complex operation, length consuming time, excess waste resource, are inappropriate for routine inspection.

Summary of the invention

The detection kit that the purpose of this invention is to provide a kind of B2M.This kit can directly apply on the automatic clinical chemistry analyzer, accuracy is strong as a result, reagent stability good, easy to use, be convenient to large-scale promotion.

Another object of the present invention provides the production preparation and the method for application of above-mentioned detection kit.

For realizing above-mentioned purpose, kit provided by the invention consists of:

Reagent R1: damping fluid, polyglycol, Sodium azide, sodium ethylene diamine tetracetate, bovine serum albumin(BSA);

Reagent R2: damping fluid, the latex particle that is combined with the B2M monoclonal antibody, Tween-20, Sodium azide, sodium ethylene diamine tetracetate, bovine serum albumin(BSA).

It is following that the preparation method of mentioned reagent box provided by the invention and behaviour do step:

(a) prepare reagent in following ratio:

Reagent R1:

Tris damping fluid 200-220mmol/L

Polyglycol 5-10 mmol/L

Sodium azide 1-5 mmol/L

Sodium ethylene diamine tetracetate 1-5mmol/L

Bovine serum albumin(BSA) 1-5mmol/L;

Reagent R2:

Tris damping fluid 200-220mmol/L

Be combined with the latex particle 3.5-5% (v/v) of B2M monoclonal antibody

Tween-20 1-5mmol/L

Sodium azide 0.5-3 mmol/L

Sodium ethylene diamine tetracetate 1-5mmol/L

Bovine serum albumin(BSA) 1-5mmol/L.

(b) reagent is mixed with sample to be tested by a certain percentage, make its complete reaction;

(c) measure the absorbance changing value with half/automatic clinical chemistry analyzer;

(d) calculate the concentration of B2M in the sample according to the absorbance changing value.

Embodiment

Following embodiment is for the more detailed the present invention of explanation, is confined to this but should not be construed as the present invention.

Embodiment 1:

Kit of the present invention is double reagent for example, wherein:

Reagent R1:

Tris damping fluid 200mmol/L

Polyglycol 8 mmol/L

Sodium azide 5 mmol/L

Sodium ethylene diamine tetracetate 5mmol/L

Bovine serum albumin(BSA) 5mmol/L;

Reagent R2:

Tris damping fluid 200mmol/L

Be combined with the latex particle 5% (v/v) of B2M monoclonal antibody

Tween-20 5mmol/L

Sodium azide 3 mmol/L

Sodium ethylene diamine tetracetate 5mmol/L

Bovine serum albumin(BSA) 5mmol/L.

Embodiment 2: the kit method of application

1. reagent is prepared, and reagent is liquid double reagent, and uncork i.e. usefulness, wherein:

Reagent R1:

Tris damping fluid 200mmol/L

Polyglycol 8 mmol/L

Sodium azide 5 mmol/L

Sodium ethylene diamine tetracetate 5mmol/L

Bovine serum albumin(BSA) 5mmol/L;

Reagent R2:

Tris damping fluid 200mmol/L

Be combined with the latex particle 5% (v/v) of B2M monoclonal antibody

Tween-20 5mmol/L

Sodium azide 3 mmol/L

Sodium ethylene diamine tetracetate 5mmol/L

Bovine serum albumin(BSA) 5mmol/L.

2. the full-automatic biochemical instrument parameter is provided with

(a) detected temperatures: 37 ℃

(b) detect wavelength: predominant wavelength is 550nm; Commplementary wave length is 700 nm;

(c) reaction time: 8 minutes, wherein, 5 minutes incubation time, 0.5 minute reaction time, 2.5 minutes detection times.

3. detect step (all in automatic clinical chemistry analyzer, accomplishing)

(a) get 240 μ L reagent 1 and 3 μ L serum sample mixings.

(b) with the solution behind the mixing 37 ℃ of incubations 5 minutes.

(c) add 60 μ L reagent 2, react after 0.5 minute, the absorbance that under the 550nm condition, detects 2.5 minutes changes.

4. the concentration that calculates B2M that changes through absorbance.

The advantage that the present invention has:

Accuracy is strong as a result, reagent stability good for this kit, easy to use, be convenient to large-scale promotion.Required detecting instrument (Biochemical Analyzer) generally uses at various big hospital and inspection center.

Claims

1. B2M detection kit, it consists of: reagent R1, reagent R2, wherein:

A, reagent R1 comprise: 200-220mmol/L Tris damping fluid, 5-10 mmol/L polyglycol, 1-5 mmol/L Sodium azide, 1-5mmol/L sodium ethylene diamine tetracetate, 1-5mmol/L bovine serum albumin(BSA);

B, reagent R2:200-220mmol/L Tris damping fluid, the latex particle that is combined with the B2M monoclonal antibody, 1-5mmol/L Tween-20,0.5-3 mmol/L Sodium azide, 1-5mmol/L sodium ethylene diamine tetracetate, 1-5mmol/L bovine serum albumin(BSA).

2. the kit of claim 1; It is characterized in that: the preparation method of the latex particle of the B2M monoclonal antibody among the reagent R2 is: the B2M monoclonal antibody of 10.5mg/ml and 10% latex particle (diameter 50-100nm); Join in the 200-220mmol/L Tris damping fluid concussion reaction 4 hours, the centrifugal supernatant that goes; Be diluted to 0.5% with 200-220mmol/L Tris damping fluid again, and add 0.1-0.3 mmol/L Sodium azide.

3. the preparation of mentioned reagent box and method of operating, key step is:

(a) prepare reagent in following ratio:

Reagent R1:

Tris damping fluid 200-220mmol/L

Polyglycol 5-10 mmol/L

Sodium azide 1-5 mmol/L

Sodium ethylene diamine tetracetate 1-5mmol/L

Bovine serum albumin(BSA) 1-5mmol/L;

Reagent R2:

Tris damping fluid 200-220mmol/L

Be combined with the latex particle 3.5-5% (v/v) of B2M monoclonal antibody

Tween-20 1-5mmol/L

Sodium azide 0.5-3 mmol/L

Sodium ethylene diamine tetracetate 1-5mmol/L

Bovine serum albumin(BSA) 1-5mmol/L

4. the method for claim 3 is characterized in that, sample and ratio of reagents should be controlled at 1:90 between the 1:110.

5. the method for claim 3 is characterized in that, temperature of reaction is 37 (± 1) ℃.

6. the method for claim 3 is characterized in that, the reaction time is 8-10 minute.

7. the method for claim 3 is characterized in that, the predominant wavelength of using half/automatic clinical chemistry analyzer to detect is 550nm, and commplementary wave length is 700 nm.