CN102749321A - Biological particle inspection device - Google Patents
Biological particle inspection device Download PDFInfo
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- CN102749321A CN102749321A CN2011101003234A CN201110100323A CN102749321A CN 102749321 A CN102749321 A CN 102749321A CN 2011101003234 A CN2011101003234 A CN 2011101003234A CN 201110100323 A CN201110100323 A CN 201110100323A CN 102749321 A CN102749321 A CN 102749321A
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- flow measurement
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Abstract
The invention discloses a biological particle inspection device, which comprises: a sample liquid reaction cup used for accommodating a sample liquid; a duct, which has one end inserted into the sample liquid reaction cup and the other end connected with a waste liquid bottle, and passes through a flow measurement chamber; the flow measurement chamber, two sides of which are respectively provided with a positive electrode and a negative electrode; a magnet, which is arranged below the negative electrode and adsorbs magnetic microspheres attached with biological particles in the sample liquid to the electrodes; a peristaltic pump that is connected to the duct and used for providing power; a electron donor, which is disposed in the flow measurement chamber and can make the biological particles attached to the magnetic microspheres generate sustained optical signals when voltage is applied to the positive electrode and the negative electrode ends; a photomultiplier tube, which conducts AD conversion on the optical signals and transmits them to a micro-control circuit; and the micro-control circuit, which compares a result with the data of a database and judges characteristics of the biological particles.
Description
Technical field
The present invention relates to field of biological detection, in particular to a kind of biomone verifying attachment.
Background technology
The research of magnetic micro-beads starts from the seventies in 20th century; It is except the character with general magnetic micro-beads; Can also give magnetic micro-beads surface various active functional group through chemical reactions such as copolymerization and surface modifications; As-COOH ,-COH ,-NH2 ,-OH etc., also can combine bioactivators such as bio-hormone, cell, antibody through covalent bond, and can conveniently under the effect of externally-applied magnetic field promptly locate, lead and separate.Compare with traditional isolation technics, this method will be separated with enrichment and will be incorporated into one, and its bigger surface area has improved in the detachment process interactional dynamics speed between the reactant greatly, have efficient, quick, non-advantage such as stain.
In recent years, radioimmunology (RIA) detection of biological particle cost is lower, but long because of this method report time, unstable result, and have the isotopic contamination problem has been tending towards superseded abroad.That colloid gold label immune chromatography test paper detection technique has is simple and convenient, reaction fast, characteristics such as identification are widely used in fields such as medical science detection, biological and chemical check easily.
Colloid gold label immune chromatography test paper detection technique both had been applicable to the naked eyes visual determination, but also instrument identification, but have can not quantitative measurment for visual determination, shortcoming that can only observational measurement, and the objectivity of measurement result is relatively poor.
Summary of the invention
The present invention provides a kind of biomone verifying attachment, is which kind of pathogeny microorganism in order to judge biomone rapidly and accurately.
For achieving the above object; The invention provides a kind of biomone verifying attachment; It comprises: appearance liquid reaction cup, conduit, peristaltic pump, waste liquid bottle, flow measurement chamber, positive electrode, negative electrode, magnet, photomultiplier and micro-control circuit; Wherein, appearance liquid reaction cup is used to hold a kind liquid, and biomone and magnetic microballon in the appearance liquid react in appearance liquid reaction cup; One end of conduit inserts in the appearance liquid reaction cup; Its other end connects waste liquid bottle; Conduit passes the flow measurement chamber; Flow measurement chamber both sides are separately installed with positive electrode and negative electrode, and magnet is arranged on the negative electrode below, and the magnetic microballon of settled organism particle is adsorbed onto on the electrode by the magnet under the negative electrode in the appearance liquid; Peristaltic pump is connected on the conduit, is used to provide power, makes reacted appearance liquid get into the flow measurement chamber through conduit, and the magnetic microballon of settled organism particle is adsorbed onto on the electrode by the magnet under the negative electrode in the appearance liquid; Be placed with electron donor in the flow measurement chamber, when positive electrode and negative electrode two ends making alive, electron donor makes the biomone of the magnetic bead attachment that is adsorbed on the electrode produce the light signal that continues; Photomultiplier carries out the AD conversion and the result is sent to micro-control circuit light signal; Micro-control circuit compares result and database data, judges the characteristic of biomone, to determine that it is which kind of pathogeny microorganism.
The foregoing description utilizes the enrichment and the separating property of magnetic microballon; The biomone that will adhere on the magnetic microballon through magnet is adsorbed onto on the electrode; The light signal that biomone is produced under the electron donor effect converts digital signal into; And with its with in advance the storage database data compare, thereby can accurately detect the biomone characteristic more quickly, overcome the problem that exists in the prior art.
Description of drawings
In order to be illustrated more clearly in the embodiment of the invention or technical scheme of the prior art; To do to introduce simply to the accompanying drawing of required use in embodiment or the description of the Prior Art below; Obviously, the accompanying drawing in describing below only is some embodiments of the present invention, for those of ordinary skills; Under the prerequisite of not paying creative work property, can also obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is biomone verifying attachment synoptic diagram according to an embodiment of the invention.
Embodiment
To combine the accompanying drawing in the embodiment of the invention below, the technical scheme in the embodiment of the invention is carried out clear, intactly description, obviously, described embodiment only is the present invention's part embodiment, rather than whole embodiment.Based on the embodiment among the present invention, those of ordinary skills are not paying the every other embodiment that is obtained under the creative work prerequisite, all belong to the scope of the present invention's protection.
Fig. 1 is biomone verifying attachment synoptic diagram according to an embodiment of the invention.As shown in Figure 1; It comprises: appearance liquid reaction cup 1, conduit 7, peristaltic pump 5, waste liquid bottle 6, flow measurement chamber 4, positive electrode 2, negative electrode 9, magnet 3, photomultiplier 8 and micro-control circuit (not shown); Wherein appearance liquid reaction cup 1 is used to hold a kind liquid, and biomone and magnetic microballon in the appearance liquid react in appearance liquid reaction cup 1;
One end of conduit 7 inserts in the appearance liquid reaction cup 1; Its other end connects waste liquid bottle 6; Conduit 7 passes flow measurement chamber 4; Flow measurement chamber 4 both sides are separately installed with positive electrode 2 and negative electrode 9, and magnet 3 is arranged on negative electrode 9 belows, and the magnetic microballon of settled organism particle is adsorbed onto on the electrode by the magnet under the negative electrode 93 in the appearance liquid;
Peristaltic pump 5 is connected on the conduit 7, is positioned at after the flow measurement chamber 4, is used to provide power, makes reacted appearance liquid get into flow measurement chamber 4 through conduit 7, and the magnetic microballon of settled organism particle is adsorbed onto on the electrode by the magnet under the negative electrode 93 in the appearance liquid;
Be placed with electron donor in the flow measurement chamber 4, when positive electrode 2 and negative electrode 9 two ends making alives, electron donor makes the biomone of the magnetic bead attachment that is adsorbed on the electrode produce the light signal that continues;
8 pairs of light signals of photomultiplier carry out the AD conversion and the result are sent to micro-control circuit;
Micro-control circuit compares result and database data, judges the characteristic of biomone, to determine that it is which kind of pathogeny microorganism.
Present embodiment utilizes the enrichment and the separating property of magnetic microballon; The biomone that will adhere on the magnetic microballon through magnet is adsorbed onto on the electrode; The light signal that biomone is produced under the electron donor effect converts digital signal into, and its database result with storage is in advance compared, and is any pathogeny microorganism thereby can accurately detect biomone more quickly; Having overcome in the prior art can not quantitative measurment; Can only observational measurement, the shortcoming that the objectivity of measurement result is relatively poor, and the dynamic process that present embodiment can the continuous monitoring biological respinse.
In addition, present embodiment utilizes the initialized data base of micro-control circuit, can detect the biomone composition more accurately; Simultaneously, owing to adopt the detection method based on magnetic microballon technology, environmentally safe.
In the above-described embodiments, peristaltic pump 5 is positioned at after the flow measurement chamber 4.
In the above-described embodiments, be not discharged in the waste liquid bottle 6 by other materials of magnet absorption.After obtaining testing result, remove the voltage at electrode two ends, remove the magnetic microballon, add the mobile measuring chamber of cleaning fluid flushing, can prepare the mensuration of next sample.
Can find out that from foregoing description the above embodiment of the present invention realizes following beneficial effect:
The appearance liquid check and analysis system of the embodiment of the invention can separate automatically, the enriched biological particle, and detects automatically and data processing, realizes the real-time online analysis to appearance liquid, thereby detects toxic ingredient more accurately, more quickly and environment is not had pollution.
One of ordinary skill in the art will appreciate that: accompanying drawing is the synoptic diagram of an embodiment, and module in the accompanying drawing or flow process might not be that embodiment of the present invention is necessary.
One of ordinary skill in the art will appreciate that: the module in the device among the embodiment can be described according to embodiment and be distributed in the device of embodiment, also can carry out respective change and be arranged in the one or more devices that are different from present embodiment.The module of the foregoing description can be merged into a module, also can further split into a plurality of submodules.
The invention described above embodiment sequence number is not represented the quality of embodiment just to description.
One of ordinary skill in the art will appreciate that: all or part of step that realizes said method embodiment can be accomplished through the relevant hardware of programmed instruction; Aforesaid program can be stored in the computer read/write memory medium; This program the step that comprises said method embodiment when carrying out; And aforesaid storage medium comprises: various media that can be program code stored such as ROM, RAM, magnetic disc or CD.
What should explain at last is: above embodiment is only in order to explaining technical scheme of the present invention, but not to its restriction; Although with reference to previous embodiment the present invention has been carried out detailed explanation, those of ordinary skill in the art is to be understood that: it still can be made amendment to the technical scheme that previous embodiment is put down in writing, and perhaps part technical characterictic wherein is equal to replacement; And these are revised or replacement, do not make the spirit and the scope of the essence disengaging embodiment of the invention technical scheme of relevant art scheme.
Claims (2)
1. biomone verifying attachment; It is characterized in that; Comprise: appearance liquid reaction cup (1), conduit (7), peristaltic pump (5), waste liquid bottle (6), flow measurement chamber (4), positive electrode (2), negative electrode (9), magnet (3), photomultiplier (8) and micro-control circuit, wherein
Said appearance liquid reaction cup (1) is used to hold a kind liquid, and biomone and magnetic microballon in the appearance liquid react in said appearance liquid reaction cup (1);
One end of said conduit (7) inserts in the said appearance liquid reaction cup (1); Its other end connects said waste liquid bottle (6); Said conduit (7) passes said flow measurement chamber (4); Said flow measurement chamber (4) both sides are separately installed with said positive electrode (2) and said negative electrode (9), and said magnet (3) is arranged on said negative electrode (9) below, and the magnetic microballon of settled organism particle is adsorbed onto on the electrode by the magnet (3) under the negative electrode (9) in the appearance liquid;
Said peristaltic pump (5) is connected on the said conduit (7); Be used to provide power; Make reacted appearance liquid get into said flow measurement chamber (4) through said conduit (7), the magnetic microballon of settled organism particle is adsorbed onto on the electrode by the said magnet (3) under the said negative electrode (9) in the appearance liquid;
Be placed with electron donor in the said flow measurement chamber (4), when said positive electrode (2) and said negative electrode (9) two ends making alive, electron donor makes the biomone of the magnetic bead attachment that is adsorbed on the electrode produce the light signal that continues;
Said photomultiplier (8) carries out the AD conversion and the result is sent to said micro-control circuit light signal;
Said micro-control circuit compares result and database data, judges the biomone characteristic.
2. biomone verifying attachment according to claim 1 is characterized in that, said peristaltic pump (5) is positioned at said flow measurement chamber (4) afterwards.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101003234A CN102749321A (en) | 2011-04-20 | 2011-04-20 | Biological particle inspection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101003234A CN102749321A (en) | 2011-04-20 | 2011-04-20 | Biological particle inspection device |
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| Publication Number | Publication Date |
|---|---|
| CN102749321A true CN102749321A (en) | 2012-10-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101003234A Pending CN102749321A (en) | 2011-04-20 | 2011-04-20 | Biological particle inspection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022033395A1 (en) * | 2020-08-14 | 2022-02-17 | 南京原码科技合伙企业(有限合伙) | Rapid concentration apparatus and method for pathogenic microorganisms |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006034525A1 (en) * | 2004-09-28 | 2006-04-06 | Cleveland Biosensors Pty Ltd | Microfluidic device |
| CN101545902A (en) * | 2008-03-24 | 2009-09-30 | 江苏省肿瘤医院 | Automatic sampling distinguishing chemiluminescent multi-component immunological detection system and analysis method of same |
| CN101672841A (en) * | 2008-09-09 | 2010-03-17 | 北京朔望科技有限公司 | Detection instrument and detection method for biological sample |
-
2011
- 2011-04-20 CN CN2011101003234A patent/CN102749321A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006034525A1 (en) * | 2004-09-28 | 2006-04-06 | Cleveland Biosensors Pty Ltd | Microfluidic device |
| CN101545902A (en) * | 2008-03-24 | 2009-09-30 | 江苏省肿瘤医院 | Automatic sampling distinguishing chemiluminescent multi-component immunological detection system and analysis method of same |
| CN101672841A (en) * | 2008-09-09 | 2010-03-17 | 北京朔望科技有限公司 | Detection instrument and detection method for biological sample |
Non-Patent Citations (2)
| Title |
|---|
| 付礼霞等: "电化学发光免疫分析与仪器的解析及临床应用", 《中国医学装备》 * |
| 冷川等: "微流控芯片上的免疫分析", 《化学进展》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022033395A1 (en) * | 2020-08-14 | 2022-02-17 | 南京原码科技合伙企业(有限合伙) | Rapid concentration apparatus and method for pathogenic microorganisms |
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Application publication date: 20121024 |