CN102746176B - Method for removing thalli in lysine fermentation liquor - Google Patents
Method for removing thalli in lysine fermentation liquor Download PDFInfo
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- CN102746176B CN102746176B CN201210251846.3A CN201210251846A CN102746176B CN 102746176 B CN102746176 B CN 102746176B CN 201210251846 A CN201210251846 A CN 201210251846A CN 102746176 B CN102746176 B CN 102746176B
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- fermentation liquor
- lysine fermentation
- temperature
- thalline
- turbidity
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Abstract
The invention discloses a method for removing thalli in lysine fermentation liquor. The method comprises the steps of carrying out heat preservation to the lysine fermentation liquor for 100-140min under the condition that the potential of hydrogen (pH) value is 3-4 and the temperature is 90-120 DEG C and then conducting solid-liquid separation. The method can achieve the purpose of obviously reducing turbidity of the thalli-removed lysine fermentation liquor, the turbidity of the thalli-removed lysine fermentation liquor basically does not change along prolonging of time and dropping of temperature, and the effect for reducing the turbidity of the thalli-removed lysine fermentation liquor is obvious particularly when the lysine fermentation liquor is subjected to heat preservation for 110-130min under the condition that the pH value is 3.3-3.8 and the temperature is 95-105 DEG C. In addition, the method is simple in operation and low in energy consumption, and can be widely used in industrial production due to no need for adding flocculating agents.
Description
Technical field
The present invention relates to a kind of method of removing thalline in lysine fermentation liquor.
Background technology
The lysine fermentation liquor complicated component obtaining after fermentation ends, the Methionin that contains 100-140g in general every liter of lysine fermentation liquor, also contains other a small amount of amino acid.In addition, in the lysine fermentation liquor obtaining after fermentation ends, the content of thalline is generally 15-30g/L, and exists with colloidal.The pH value of lysine fermentation liquor is generally 6.6-7.0.
From fermented liquid, extract Methionin, need first the thalline in fermented liquid to be removed, in prior art, the method for removing thalline in lysine fermentation liquor generally comprises: membrane filtration, centrifugal separation, interpolation flocculation agent settling process etc.Wherein, centrifugal separation investment is large, and energy consumption is high, and the filter cake that compression forms very easily stops up filter cloth, and filtration velocity and filtrate quality are affected; Adding flocculation agent method needs supernatant liquor quality to meet the requirements, and the use of flocculation agent simultaneously need to take into full account the relevant institutions that whether meets food safety.Therefore, the method generally adopting is now membrane filtration, but this method also has the drawback of himself: membrane filtration liquid is with the prolongation of storage period and the reduction of temperature, and turbidity raises, and affects the extraction effect of postorder Methionin.
Summary of the invention
To the object of the invention is the defect of removing the method for thalline in lysine fermentation liquor in prior art in order overcoming, to provide a kind of and can take into account the lysine fermentation liquor that makes to remove after thalline and have that low turbidity, removal method energy consumption are low, method simple, without the method for adding flocculation agent and removing thalline in the new removal lysine fermentation liquor that the turbidity of the lysine fermentation liquor of thalline do not change substantially along with the prolongation of storage period and the reduction of temperature.
The present inventor surprisingly finds under study for action, be that the turbidity that carries out the lysine fermentation liquor of removing thalline that solid-liquid separation obtains after being incubated 100-140min under the condition of 90-120 DEG C reduces greatly for 3-4 and temperature in pH value by lysine fermentation liquor, and along with the prolongation of storage period and the reduction of temperature, the turbidity of the lysine fermentation liquor of removing thalline obtaining does not change substantially.
Therefore, to achieve these goals, the invention provides a kind of method of removing thalline in lysine fermentation liquor, wherein, described method comprises by lysine fermentation liquor being to carry out solid-liquid separation after being incubated 100-140min under the condition of 90-120 DEG C for 3-4 and temperature in pH value.
Preferably, pH value is 3.3-3.8, and temperature is 95-105 DEG C, and soaking time is 110-130min.
Preferably, the temperature of solid-liquid separation is 50-85 DEG C, and pH value is 3-4.
In removal lysine fermentation liquor provided by the invention, the method for thalline need be only after being incubated 100-140min under the condition of 90-120 DEG C, to carry out the object that solid-liquid separation can reach remarkable reduction and removes the lysine fermentation liquor turbidity of thalline for 3-4 and temperature in pH value by lysine fermentation liquor, and along with the prolongation of storage period and the reduction of temperature, the turbidity of removing the lysine fermentation liquor of thalline does not change substantially, be to be particularly incubated after 110-130min under the condition of 95-105 DEG C for 3.3-3.8 and temperature in pH value, the effect of turbidity that reduces the lysine fermentation liquor of removing thalline is more remarkable.In addition, method of the present invention is simple to operate, energy consumption is low, and without adding flocculation agent, therefore can be widely used in industrial production.
Other features and advantages of the present invention are described in detail the embodiment part subsequently.
Embodiment
Below the specific embodiment of the present invention is elaborated.Should be understood that, embodiment described herein only, for description and interpretation the present invention, is not limited to the present invention.
The invention provides a kind of method of removing thalline in lysine fermentation liquor, the method comprises by lysine fermentation liquor being to carry out solid-liquid separation after being incubated 100-140min under the condition of 90-120 DEG C for 3-4 and temperature in pH value.
In the present invention, lysine fermentation liquor refers to adopt fermentation method to prepare Methionin, the tunning obtaining after fermentation certain hour, and in every liter of lysine fermentation liquor, the content of thalline is generally 15-30g.
According to the present invention, after being incubated 100-140min under the condition of 90-120 DEG C, carry out solid-liquid separation although by lysine fermentation liquor in pH value for 3-4 and temperature be, can realize object of the present invention, significantly reduce the turbidity of the lysine fermentation liquor of removing thalline, and along with the prolongation of storage period and the reduction of temperature, the turbidity of removing the lysine fermentation liquor of thalline does not change substantially.But under preferable case, pH value is 3.3-3.8, and temperature is 95-105 DEG C, and soaking time is 110-130min, can further significantly reduce the turbidity of the lysine fermentation liquor of removing thalline.
In the present invention, without particular requirement, can adopt the conventional the whole bag of tricks in this area for the control method of lysine fermentation liquor pH value, for example, in lysine fermentation liquor, add acidic solution.Without particular requirement, can adopt the conventional various acidic solutions in this area, such as sulfuric acid, hydrochloric acid etc., preferably sulfuric acid for the kind of acidic solution.For the concentration of acidic solution without particular requirement, as long as pH value is adjusted in claimed range, but from reducing acidic solution consumption, make to regulate pH value to be considered without the aspect of considerable influence lysine concentration in lysine fermentation liquor, further preferably adopt the sulfuric acid of 98 % by weight to regulate the pH value of lysine fermentation liquor.
In the present invention, for the control method of removing temperature in the method for thalline in lysine fermentation liquor without particular requirement, can adopt the conventional the whole bag of tricks in this area, for example can adopt steam heating, water-bath or oil bath heating, or electrically heated etc., easy to operate from actual production, cost-effective aspect is considered, preferably adopt steam heating, steam-heated embodiment can be for arranging pipeline in the fermentor tank inside that holds lysine fermentation liquor, lysine fermentation liquor is heated and be incubated to passing into steam in pipeline, or can be for holding fermentor tank periphery sandwich establishment or the pipeline of described lysine fermentation liquor, lysine fermentation liquor is heated and be incubated to passing into steam in interlayer or pipeline.
The present inventor surprisingly finds under study for action, when fermented liquid after insulation is carried out to solid-liquid separation, be 50-85 DEG C in temperature, under the condition that pH value is 3-4, carry out solid-liquid separation, can make the effect of solid-liquid separation more abundant, further after reduction solid-liquid separation, remove the turbidity of the lysine fermentation liquor of thalline, be 70-80 DEG C in temperature, under the condition that pH value is 3.3-3.8, carry out solid-liquid separation, can further reduce the turbidity of removing the lysine fermentation liquor of thalline after solid-liquid separation, therefore, in the present invention, preferably, the temperature of solid-liquid separation is 50-85 DEG C, pH value is 3-4, more preferably, the temperature of solid-liquid separation is 70-80 DEG C, and pH value is 3.3-3.8.PH value while insulation due to the inventive method and the pH value of solid-liquid separation are suitable, and those skilled in the art should understand that be, in insulating process, the pH value of lysine fermentation liquor does not change substantially, therefore, after insulation, only need to regulate the temperature of lysine fermentation liquor, without regulating pH value, carry out solid-liquid separation.
In the present invention, without particular requirement, the conventional the whole bag of tricks in this area can be adopted for the method for solid-liquid separation, for example, one or more in sedimentation, filtration and centrifugation can be selected from.Low and good effect of separating solid from liquid from the viewpoint of energy consumption, the method for solid-liquid separation is preferably sedimentation.In the present invention, sedimentation refers to natural subsidence.Carry out under above-mentioned preferred condition in order to make solid-liquid separation, be 50-85 DEG C in temperature, pH value is 3-4, be preferably 70-80 DEG C in temperature, under the condition that pH value is 3.3-3.8, carry out sedimentation, so that the better effects if of sedimentation, while adopting steam heating, after insulation, can pass through steam regulation temperature and intake, make the temperature of the lysine fermentation liquor after insulation meet the above-mentioned optimum condition of sedimentation.The time of sedimentation is preferably 20-40min.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characterictic described in above-mentioned embodiment, in reconcilable situation, can combine by any suitable mode, for fear of unnecessary repetition, the present invention is to the explanation no longer separately of various possible array modes.
In addition, also can carry out arbitrary combination between various embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Embodiment
The present invention is further illustrated for following embodiment, but therefore do not limit the present invention.
In following embodiment and comparative example:
Adopt the thalline content in gravimetric determination lysine fermentation liquor: get lysine fermentation liquor 100mL, be placed in the sample bottle of whizzer, select centrifugal 5 minutes of 4000RPM; After centrifugal, supernatant liquor is gone, the 100mL that adds water, mixes thalline with glass stick with water, and with 4000RPM centrifugal 5 minutes, supernatant liquor is gone after centrifugal, then the 100mL that adds water, repeat aforesaid operations totally three times, i.e. water repeated washing three times.Thalline is transferred to the glass surface ware of constant weight, and (weight is m
0) in, be placed in baking oven, dry to constant weight (m at 105 DEG C
1).Thalline content %=((m
1-m
0)/0.1) × 100%, unit is g/L.
Adopt WGZ-200 turbidimeter (Shanghai Precision Scientific Apparatus Co., Ltd) to measure the turbidity of the lysine fermentation liquor of removing thalline.
Embodiment 1
The present embodiment is for illustrating the method for removal lysine fermentation liquor thalline provided by the invention.
Regulating the pH value of lysine fermentation liquor (thalline content 20g/L) with the sulfuric acid of 98 % by weight is 3.5, in the fermentor tank that holds described lysine fermentation liquor, pipeline is set, in pipeline, pass into steam, making the temperature of lysine fermentation liquor by steam regulation temperature and intake is 100 DEG C, and be incubated 120min at this temperature, then to make the temperature of lysine fermentation liquor be 75 DEG C for steam regulation temperature and intake, at this temperature, after sedimentation 30min, get supernatant liquor (removing the lysine fermentation liquor of thalline after being solid-liquid separation, lower same).Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Embodiment 2
The present embodiment is for illustrating the method for removal lysine fermentation liquor thalline provided by the invention.
Regulating the pH value of lysine fermentation liquor (thalline content 15g/L) with the sulfuric acid of 98 % by weight is 3.3, in the fermentor tank that holds described lysine fermentation liquor, pipeline is set, in pipeline, pass into steam, making the temperature of lysine fermentation liquor by steam regulation temperature and intake is 95 DEG C, and be incubated 130min at this temperature, then to make the temperature of lysine fermentation liquor be 70 DEG C for steam regulation temperature and intake, at this temperature, after sedimentation 20min, gets supernatant liquor.Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Embodiment 3
The present embodiment is for illustrating the method for removal lysine fermentation liquor thalline provided by the invention.
Regulating the pH value of lysine fermentation liquor (thalline content 30g/L) with the sulfuric acid of 98 % by weight is 3.8, in the fermentor tank that holds described lysine fermentation liquor, pipeline is set, in pipeline, pass into steam, making the temperature of lysine fermentation liquor by steam regulation temperature and intake is 105 DEG C, and be incubated 110min at this temperature, then to make the temperature of lysine fermentation liquor be 80 DEG C for steam regulation temperature and intake, at this temperature, after sedimentation 40min, gets supernatant liquor.Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Embodiment 4
The present embodiment is for illustrating the method for removal lysine fermentation liquor thalline provided by the invention.
Remove the thalline in lysine fermentation liquor according to the method for embodiment 1, different, regulating the pH value of lysine fermentation liquor (thalline content 20g/L) with the sulfuric acid of 98 % by weight is 3.Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Embodiment 5
The present embodiment is for illustrating the method for removal lysine fermentation liquor thalline provided by the invention.
Remove the thalline in lysine fermentation liquor according to the method for embodiment 1, different, regulating the pH value of lysine fermentation liquor (thalline content 20g/L) with the sulfuric acid of 98 % by weight is 4.Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Embodiment 6
The present embodiment is for illustrating the method for removal lysine fermentation liquor thalline provided by the invention.
Remove the thalline in lysine fermentation liquor according to the method for embodiment 1, different, making the temperature of lysine fermentation liquor by steam regulation temperature and intake is 90 DEG C, and is incubated 140min at this temperature.Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Embodiment 7
The present embodiment is for illustrating the method for removal lysine fermentation liquor thalline provided by the invention.
Remove the thalline in lysine fermentation liquor according to the method for embodiment 1, different, making the temperature of lysine fermentation liquor by steam regulation temperature and intake is 120 DEG C, and is incubated 100min at this temperature.Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Embodiment 8
The present embodiment is for illustrating the method for removal lysine fermentation liquor thalline provided by the invention.
Remove the thalline in lysine fermentation liquor according to the method for embodiment 1, different, it is 50 DEG C that the rear steam regulation temperature of insulation and intake make the temperature of lysine fermentation liquor.Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Embodiment 9
The present embodiment is for illustrating the method for removal lysine fermentation liquor thalline provided by the invention.
Remove the thalline in lysine fermentation liquor according to the method for embodiment 1, different, it is 85 DEG C that the rear steam regulation temperature of insulation and intake make the temperature of lysine fermentation liquor.Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Comparative example 1
This comparative example is removed the thalline of lysine fermentation liquor for membrane filtration is described.
Regulating the pH value of lysine fermentation liquor (thalline content 20g/L) with the sulfuric acid of 98 % by weight is 2.5, then make lysine fermentation liquor (be purchased from Hefei Shi Jiemo engineering limited liability company by ceramic membrane at 80 DEG C, membrane pore size: m), obtain Methionin membrane filtration liquid is Methionin clear liquid to 0.1 μ.Temperature and the turbidity of measuring membrane filtration liquid every 10min, the results are shown in Table 1.
Comparative example 2
This comparative example is for illustrating the thalline that adds flocculation agent settling process removal lysine fermentation liquor.
At the lysine fermentation liquors of 50 DEG C (thalline content 20g/L, pH value is 3.5) in add polyacrylamide (Anhui Tianrun Chemical Industry Co., Ltd., number-average molecular weight 1,200 ten thousand), with respect to the 1g thalline in fermented liquid, the add-on of flocculation agent is 10mg, stir while adding evenly, then insulation is got supernatant liquor after leaving standstill 150min.Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Comparative example 3
Remove the thalline in lysine fermentation liquor according to the method for embodiment 1, different, regulating the pH value of lysine fermentation liquor (thalline content 20g/L) with the sulfuric acid of 98 % by weight is 2; Making the temperature of lysine fermentation liquor by steam regulation temperature and intake is 80 DEG C, and is incubated 120min at this temperature.Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Comparative example 4
Remove the thalline in lysine fermentation liquor according to the method for embodiment 1, different, regulating the pH value of lysine fermentation liquor (thalline content 20g/L) with the sulfuric acid of 98 % by weight is 5; Making the temperature of lysine fermentation liquor by steam regulation temperature and intake is 130 DEG C, and is incubated 120min at this temperature.Temperature and the turbidity of after taking-up supernatant liquor, measuring supernatant liquor every 10min, the results are shown in Table 1.
Table 1
Note: in table, 0min, 10min, 20min, 30min, 40min, 50min and 60min represent to take out the time after supernatant liquor, 0min measures after representing to take out supernatant liquor immediately; In table, the unit of temperature is DEG C, and the unit of turbidity is NTU.
Embodiment 1 is compared and can be found out with comparative example 1 and comparative example 2 respectively, the inventive method has greatly reduced the turbidity of the lysine fermentation liquor of removing thalline, and along with the prolongation of storage period and the reduction of temperature, the turbidity of the lysine fermentation liquor of removing thalline obtaining does not change substantially, and the interpolation flocculation agent settling process of the membrane filtration of comparative example 1 and comparative example 2, the turbidity of the lysine fermentation liquor of removing thalline not only obtaining is higher, and along with the prolongation of storage period and the reduction of temperature, the turbidity of removing the lysine fermentation liquor of thalline raises gradually, even if removing the temperature of the lysine fermentation liquor of thalline has not changed, the turbidity of removing the lysine fermentation liquor of thalline still continues to raise, and add flocculation agent settling process and exist the problem of the food safety hidden danger of the Methionin that subsequent extracted goes out, embodiment 1 is compared and can be found out with comparative example 3, pH value when insulation and temperature are lower than scope of the present invention, can not significantly reduce the turbidity of the lysine fermentation liquor of removing thalline, and along with the prolongation of storage period and the reduction of temperature, the turbidity of removing the lysine fermentation liquor of thalline raises gradually, do not changed even if remove the temperature of the lysine fermentation liquor of thalline, the turbidity of removing the lysine fermentation liquor of thalline still continues to raise, and turbidity is unstable.Embodiment 1 is compared and can be found out with comparative example 4, pH value when insulation and temperature are higher than scope of the present invention, although along with the prolongation of storage period and the reduction of temperature, the turbidity of the lysine fermentation liquor of removing thalline obtaining does not change substantially, but the turbidity of the lysine fermentation liquor of removing thalline obtaining is higher, measured value when 0min is apparently higher than membrane filtration and add flocculation agent settling process.
Embodiment 1 is compared and can be found out with embodiment 4 and embodiment 5 respectively, and when the pH value during by insulation is controlled in preferable range of the present invention, the removal effect of thalline is more remarkable; Embodiment 1 is compared and can be found out with embodiment 6 and embodiment 7 respectively, and when the temperature during by insulation is controlled in preferable range of the present invention, the removal effect of thalline is more remarkable; Embodiment 1 is compared and can be found out with embodiment 8 and embodiment 9 respectively, and when the temperature of solid-liquid separation is controlled in preferable range of the present invention, the removal effect of thalline is more remarkable.
The object that in removal lysine fermentation liquor provided by the invention, the method for thalline can reach remarkable reduction and removes the turbidity of the lysine fermentation liquor of thalline, and along with the prolongation of storage period and the reduction of temperature, the turbidity of removing the lysine fermentation liquor of thalline does not change substantially, be to be particularly incubated after 110-130min under the condition of 95-105 DEG C for 3.3-3.8 and temperature in pH value, the effect of turbidity that reduces the lysine fermentation liquor of removing thalline is more remarkable.In addition, method of the present invention is simple to operate, energy consumption is low, and without adding flocculation agent, therefore can be widely used in industrial production.
Claims (4)
1. remove the method for thalline in lysine fermentation liquor for one kind, it is characterized in that, described method comprises by lysine fermentation liquor being to carry out solid-liquid separation after being incubated 100-140min under the condition of 90-120 DEG C for 3-4 and temperature in pH value, the temperature of described solid-liquid separation is 50-85 DEG C, pH value is 3-4, and in every liter of described lysine fermentation liquor, the content of thalline is 15-30g.
2. method according to claim 1 wherein, is to carry out solid-liquid separation after being incubated 110-130min under the condition of 95-105 DEG C for 3.3-3.8 and temperature in pH value by lysine fermentation liquor.
3. method according to claim 1 and 2, wherein, the control method of pH value for to add acidic solution in lysine fermentation liquor.
4. method according to claim 1 and 2, wherein, the method for described solid-liquid separation is selected from one or more in sedimentation, filtration and centrifugation.
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| CN102746176B true CN102746176B (en) | 2014-06-18 |
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| CN104276963B (en) * | 2013-07-01 | 2016-03-23 | 中粮生物化学（安徽）股份有限公司 | A kind for the treatment of process of amino acid fermentation mash |
| CN104276967B (en) * | 2013-07-01 | 2017-04-12 | 中粮生物化学（安徽）股份有限公司 | Method for producing low-lysine -content product |
| CN103450041A (en) * | 2013-08-20 | 2013-12-18 | 长治市金泽生物工程有限公司 | Extraction process of lysine fermentation liquor |
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|---|---|---|---|---|
| US20040137571A1 (en) * | 2002-11-26 | 2004-07-15 | Jan Markussen | Method for purifying a fermentation-derived product |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040137571A1 (en) * | 2002-11-26 | 2004-07-15 | Jan Markussen | Method for purifying a fermentation-derived product |
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